1,171
Views
23
CrossRef citations to date
0
Altmetric
Short Technical Reports

“Lumen digestion” technique for isolation of aortic endothelial cells from heme oxygenase-1 knockout mice

, &
Pages 84-89 | Received 20 Feb 2004, Accepted 12 Apr 2004, Published online: 06 Jun 2018

References

  • Dong, Q.G., S.Bernasconi, S.Lostaglio, R.W.De Calmanovici, I.Martin-Padura, F.Breviario, C.Garlanda, S.Ramponi, et al.. 1997. A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants. Arterioscler. Thromb. Vasc. Biol.17:1599–1604.
  • Gerritsen, M.E., C.P.Shen, W.J.Atkinson, R.C.Padgett, M.A.Gimbrone, Jr., and D.S.Milstone. 1996. Microvascular endothelial cells from E-selectin-deficient mice form tubes in vitro. Lab. Invest.75:175–184.
  • Gerritsen, M.E., C.P.Shen, M.C.McHugh, W.J.Atkinson, J.M.Kiely, D.S.Milstone, F.W.Luscinskas, and M.A.Gimbrone, Jr. 1995. Activation-dependent isolation and culture of murine pulmonary microvascular endothelium. Microcirculation2:151–163.
  • Kevil, C.G. and D.C.Bullard. 2001. In vitro culture and characterization of gene targeted mouse endothelium. Acta Physiol. Scand.173:151–157.
  • Magid, R., D.Martinson, J.Hwang, H.Jo, and Z.S.Galis. 2003. Optimization of isolation and functional characterization of primary murine aortic endothelial cells. Endothelium10:103–109.
  • Shi, W., M.E.Haberland, M.L.Jien, D.M.Shih, and A.J.Lusis. 2000. Endothelial responses to oxidized lipoproteins determine genetic susceptibility to atherosclerosis in mice. Circulation102:75–81.
  • Suh, S.H., R.Vennekens, V.G.Manolopoulos, M.Freichel, U.Schweig, J.Prenen, V.Flockerzi, G.Droogmans, and B.Nilius. 1999. Characterisation of explanted endothelial cells from mouse aorta: electrophysiology and Ca2+ signalling. Pflugers Arch.438:612–620.
  • Wu, Z., F.M.Hofman, and B.V.Zlokovic. 2003. A simple method for isolation and characterization of mouse brain microvascular endothelial cells. J. Neurosci. Methods130:53–63.
  • Poss, K.D. and S.Tonegawa. 1997. Heme oxygenase 1 is required for mammalian iron reutilization. Proc. Natl. Acad. Sci. USA94:10919–10924.
  • Feng, D., J.A.Nagy, K.Pyne, H.F.Dvorak, and A.M.Dvorak. 2004. Ultrastructural localization of platelet endothelial cell adhesion molecule (PECAM-1, CD31) in vascular endothelium. J. Histochem. Cytochem.52:87–101.
  • Agarwal, A., F.Shiraishi, G.A.Visner, and H.S.Nick. 1998. Linoleyl hydroperoxide transcriptionally upregulates heme oxygenase-1 gene expression in human renal epithelial and aortic endothelial cells. J. Am. Soc. Nephrol.9:1990–1997.
  • Barkalow, F.J., M.J.Goodman, and T.N.Mayadas. 1996. Cultured murine cerebral microvascular endothelial cells contain von Willebrand factor-positive Weibel-Palade bodies and support rapid cytokine-induced neutrophil adhesion. Microcirculation3:19–28.
  • Durante, W. and A.I.Schafer. 1998. Carbon monoxide and vascular cell function (Review). Int. J. Mol. Med.2:255–262.
  • Exner, M., M.Schillinger, E.Minar, W.Mlekusch, G.Schlerka, M.Haumer, C.Mannhalter, and O.Wagner. 2001. Heme oxygenase-1 gene promoter microsatellite polymorphism is associated with restenosis after percutaneous transluminal angioplasty. J. Endovasc. Ther.8:433–440.
  • Ishikawa, K., D.Sugawara, X.Wang, K.Suzuki, H.Itabe, Y.Maruyama, and A.J.Lusis. 2001. Heme oxygenase-1 inhibits atherosclerotic lesion formation in ldl-receptor knockout mice. Circ. Res.88:506–512.
  • Juan, S.H., T.S.Lee, K.W.Tseng, J.Y.Liou, S.K.Shyue, K.K.Wu, and L.Y.Chau. 2001. Adenovirus-mediated heme oxygenase-1 gene transfer inhibits the development of atherosclerosis in apolipoprotein E-deficient mice. Circulation104:1519–1525.
  • Kaneda, H., M.Ohno, J.Taguchi, M.Togo, H.Hashimoto, K.Ogasawara, T.Aizawa, N.Ishizaka, and R.Nagai. 2002. Heme oxygenase-1 gene promoter polymorphism is associated with coronary artery disease in Japanese patients with coronary risk factors. Arterioscler. Thromb. Vasc. Biol.22:1680–1685.
  • Otterbein, L.E., B.S.Zuckerbraun, M.Haga, F.Liu, R.Song, A.Usheva, C.Stachulak, N.Bodyak, et al.. 2003. Carbon monoxide suppresses arteriosclerotic lesions associated with chronic graft rejection and with balloon injury. Nat. Med.9:183–190.
  • Tulis, D.A., W.Durante, K.J.Peyton, A.J.Evans, and A.I.Schafer. 2001. Heme oxygenase-1 attenuates vascular remodeling following balloon injury in rat carotid arteries. Atherosclerosis155:113–122.
  • Yet, S.F., M.D.Layne, X.Liu, Y.H.Chen, B.Ith, N.E.Sibinga, and M.A.Perrella. 2003. Absence of heme oxygenase-1 exacerbates atherosclerotic lesion formation and vascular remodeling. FASEB J.17:1759–1761.