3,045
Views
5
CrossRef citations to date
0
Altmetric
Reports

Single-cell Analysis for Drug Development Using Convex Lens-Induced Confinement Imaging

, , , , &
Pages 210-217 | Received 31 May 2019, Accepted 26 Jul 2019, Published online: 28 Oct 2019

References

  • Abdallah BY , HorneSD, StevensJBet al. Single cell heterogeneity: why unstable genomes are incompatible with average profiles. Cell Cycle12(23), 3640–3649 (2013).
  • Heng HH , BremerSW, StevensJBet al. Chromosomal instability (CIN): what it is and why it is crucial to cancer evolution. Cancer Metastasis Rev.32(3–4), 325–340 (2013).
  • Elowitz MB , LevineAJ, SiggiaED, SwainPS. Stochastic gene expression in a single cell. Science297(5584), 1183–1186 (2002).
  • Balaban NQ , MerrinJ, ChaitR, KowalikL, LeiblerS. Bacterial persistence as a phenotypic switch. Science305(5690), 1622–1625 (2004).
  • Baltekin Ö , BoucharinA, TanoE, AnderssonDI, ElfJ. Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging. Proc. Natl Acad. Sci. USA114(34), 9170–9175 (2017).
  • Dai J , HamonM, JambovaneS. Microfluidics for antibiotic susceptibility and toxicity testing. Bioengineering (Basel)3(4), E25 (2016).
  • Leslie SR , FieldsAP, CohenAE. Convex lens-induced confinement for imaging single molecules. Anal. Chem.82(14), 6224–6229 (2010).
  • Berard D , McFaulCM, LeithJS, ArsenaultAK, MichaudF, LeslieSR. Precision platform for convex lens-induced confinement microscopy. Rev. Sci. Instrum.84(10), 103704 (2013).
  • Berard DJ , ShayeganM, MichaudF, HenkinG, ScottS, LeslieS. Formatting and ligating biopolymers using adjustable nanoconfinement. Appl. Physics Lett.109(3), 033702 (2016).
  • Scott S , XuZM, KouzineFet al. Visualizing structure-mediated interactions in supercoiled DNA molecules. Nucleic Acids Res.46(9), 4622–4631 (2018).
  • Scott S , ShaheenC, McGuinnessBet al. Single-molecule visualization of the effects of ionic strength and crowding on structure-mediated interactions in supercoiled DNA molecules. Nucleic Acids Res.47(12), 6360–6368 (2019).
  • Shayegan M , TahvildariR, MeteraK, KisleyL, MichnickSW, LeslieSR. probing inhomogeneous diffusion in the microenvironments of phase-separated polymers under confinement. J. Am. Chem. Soc.141(19), 7751–7757 (2019).
  • Jia B , WeeTL, BoudreauCGet al. Parallelized cytoindentation using convex micropatterned surfaces. BioTechniques61(2), 73–82 (2016).
  • Simon JA , BedalovA. Yeast as a model system for anticancer drug discovery. Nat. Rev. Cancer4(6), 481–492 (2004).
  • Giaever G , NislowC. The yeast deletion collection: a decade of functional genomics. Genetics197(2), 451–465 (2014).
  • Lee AY , StOnge RP, ProctorMJet al. Mapping the cellular response to small molecules using chemogenomic fitness signatures. Science344(6180), 208–211 (2014).
  • Brachmann CB , DaviesA, CostGJet al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast14(2), 115–132 (1998).
  • Schneider CA , RasbandWS, EliceiriKW. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods9(7), 671–675 (2012).
  • Schindelin J , Arganda-CarrerasI, FriseEet al. Fiji: an open-source platform for biological-image analysis. Nat. Methods9(7), 676–682 (2012).
  • Wong LH , SinhaS, BergeronJRet al. Reverse chemical genetics: comprehensive fitness profiling reveals the spectrum of drug target interactions. PLoS Genet.12(9), e1006275 (2016).
  • Lagosky PA , TaylorGR, HaynesRH. Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1). Nucleic Acids Res.15(24), 10355–10371 (1987).
  • Altmann K , WestermannB. Role of essential genes in mitochondrial morphogenesis in Saccharomyces cerevisiae. Mol. Biol. Cell16(11), 5410–5417 (2005).
  • Zettel MF , GarzaLR, CassAMet al. The budding index of Saccharomyces cerevisiae deletion strains identifies genes important for cell cycle progression. FEMS Microbiol. Lett.223(2), 253–258 (2003).
  • Bi E , ParkHO. Cell polarization and cytokinesis in budding yeast. Genetics.191(2), 347–387 (2012).
  • Jonczyk R , KurthT, LavrentievaA, WalterJG, ScheperT, StahlF. Living cell microarrays: an overview of concepts. Microarrays (Basel).5(2), (2016).