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Reports

Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues

ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon
Pages 153-160 | Received 12 Sep 2023, Accepted 22 Dec 2023, Published online: 09 Feb 2024

References

  • Chomczynski P , SacchiN. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat. Protoc.1(2), 581–585 (2006).
  • Foss RD , Guha-ThakurtaN , ConranRM , GutmanP. Effects of fixative and fixation time on the extraction and polymerase chain reaction amplification of RNA from paraffin-embedded tissue. Comparison of two housekeeping gene mRNA controls. Diagn. Mol. Pathol.3(3), 148–155 (1994).
  • Hoffman EA , FreyBL , SmithLM , AubleDT. Formaldehyde crosslinking: a tool for the study of chromatin complexes. J. Biol. Chem.290(44), 26404–26411 (2015).
  • Masuda N , OhnishiT , KawamotoS , MondenM , OkuboK. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res.27(22), 4436–4443 (1999).
  • Soles A , SelimovicA , SbroccoKet al. Extracellular matrix regulation in physiology and in brain disease. Int. J. Mol. Sci.24(8), 7049 (2023).
  • Tasaki M , LavatelliF , ObiciLet al. Age-related amyloidosis outside the brain: a state-of-the-art review. Ageing Res. Rev.70, 101388 (2021).
  • Brack AS , ConboyMJ , RoySet al. Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis. Science317(5839), 807–810 (2007).
  • Edstrom E , AltunM , BergmanEet al. Factors contributing to neuromuscular impairment and sarcopenia during aging. Physiol. Behav.92(1–2), 129–135 (2007).
  • Thompson SD , PichikaR , LieberRL , LavasaniM. Systemic transplantation of adult multipotent stem cells prevents articular cartilage degeneration in a mouse model of accelerated ageing. Immun. Ageing18(1), 27 (2021).
  • Evers DL , FowlerCB , CunninghamBR , MasonJT , O’LearyTJ. The effect of formaldehyde fixation on RNA: optimization of formaldehyde adduct removal. J. Mol. Diagn.13(3), 282–288 (2011).
  • Xin X , JiangX , LichtlerA , KronenbergM , RoweD , PachterJS. Laser-capture microdissection and RNA extraction from perfusion-fixed cartilage and bone tissue from mice implanted with human iPSC-derived MSCs in a calvarial defect model. Methods Mol. Biol.1723, 385–396 (2018).
  • Jager SB , PallesenLT , VaegterCB. Isolation of satellite glial cells for high-quality RNA purification. J. Neurosci. Methods297, 1–8 (2018).
  • Preusser M , PlumerS , DirnbergerE , HainfellnerJA , MannhalterC. Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids. Brain Pathol.20(6), 1010–1020 (2010).
  • Groelz D , SobinL , BrantonP , ComptonC , WyrichR , RainenL. Non-formalin fixative versus formalin-fixed tissue: a comparison of histology and RNA quality. Exp. Mol. Pathol.94(1), 188–194 (2013).
  • Boos GS , NobachD , FailingK , EickmannM , HerdenC. Optimization of RNA extraction protocol for long-term archived formalin-fixed paraffin-embedded tissues of horses. Exp. Mol. Pathol.110, 104289 (2019).
  • Klopfleisch R , von DeetzenM , WeissATet al. Weigners fixative – an alternative to formalin fixation for histology with improved preservation of nucleic acids. Vet. Pathol.50(1), 191–199 (2013).
  • Wimmer I , TroscherAR , BrunnerFet al. Systematic evaluation of RNA quality, microarray data reliability and pathway analysis in fresh, fresh frozen and formalin-fixed paraffin-embedded tissue samples. Sci. Rep.8(1), 6351 (2018).
  • Medhurst AD , HarrisonDC , ReadSJ , CampbellCA , RobbinsMJ , PangalosMN. The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models. J. Neurosci. Methods98(1), 9–20 (2000).
  • Gutala RV , ReddyPH. The use of real-time PCR analysis in a gene expression study of Alzheimer’s disease post-mortem brains. J. Neurosci. Methods132(1), 101–107 (2004).
  • Bernard PS , WittwerCT. Real-time PCR technology for cancer diagnostics. Clin. Chem.48(8), 1178–1185 (2002).
  • Okamoto T , OkabeS. Ultraviolet absorbance at 260 and 280 nm in RNA measurement is dependent on measurement solution. Int. J. Mol. Med.5(6), 657–659 (2000).
  • Thompson SD , PichikaR , LieberRL , BudingerGRS , LavasaniM. Systemic transplantation of adult multipotent stem cells functionally rejuvenates aged articular cartilage. Aging Dis.12(3), 726–731 (2021).