Common molecular markers between circulating tumor cells and blood exosomes in colorectal cancer: a systematic and analytical review

References

• Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65(2):87–108. doi:10.3322/caac.2126225651787
   PubMed Web of Science ®Google Scholar
• Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015;65(1):5–29. doi:10.3322/caac.2125425559415
   PubMed Web of Science ®Google Scholar
• Punt CJ, Koopman M, Vermeulen L. From tumour heterogeneity to advances in precision treatment of colorectal cancer. Nat Rev Clin Oncol. 2017;14(4):235. doi:10.1038/nrclinonc.2016.17127922044
   PubMed Web of Science ®Google Scholar
• Zhai Z, Yu X, Yang B, et al. Colorectal cancer heterogeneity and targeted therapy: clinical implications, challenges and solutions for treatment resistance. Paper presented at:Semin Cell Dev Biol; 2017;64:107–115. doi:10.1016/j.semcdb.2016.08.033
   Google Scholar
• Bailey JR, Aggarwal A, Imperiale TF. Colorectal cancer screening: stool DNA and other noninvasive modalities. Gut Liver. 2016;10(2):204–211. doi:10.5009/gnl1542026934885
   PubMed Web of Science ®Google Scholar
• Heichman KA. Blood-based testing for colorectal cancer screening. Mol Diagn Ther. 2014;18(2):127–135. doi:10.1007/s40291-013-0074-z24307563
   PubMed Web of Science ®Google Scholar
• Hench IB, Hench J, Tolnay M. Liquid biopsy in clinical management of breast, lung, and colorectal cancer. Front Med. 2018;5:9. doi:10.3389/fmed.2018.00009
   Web of Science ®Google Scholar
• Lopez A, Harada K, Mizrak Kaya D, Dong X, Song S, Ajani JA. Liquid biopsies in gastrointestinal malignancies: when is the big day? Expert Rev Anticancer Ther. 2018;18(1):19–38. doi:10.1080/14737140.2018.1403320
   PubMed Web of Science ®Google Scholar
• Ryan E, Creagh E. Emerging methods in colorectal cancer screening. BJS. 2018;105(2):e16–e18. doi:10.1002/bjs.10650
   Google Scholar
• Hauptman N, Glavac D. Colorectal cancer blood-based biomarkers. Gastroenterol Res Pract. 2017;2017:2195361. doi:10.1155/2017/219536129147109
   PubMed Web of Science ®Google Scholar
• Shao H, Chung J, Issadore D. Diagnostic technologies for circulating tumour cells and exosomes. Biosci Rep. 2015;36(1):e00292. doi:10.1042/BSR2015018026604322
   PubMedGoogle Scholar
• Hessvik NP, Llorente A. Current knowledge on exosome biogenesis and release. Cell Mol Life Sci. 2018;75(2):193–208. doi:10.1007/s00018-017-2595-928733901
   PubMed Web of Science ®Google Scholar
• Thorsteinsson M, Jess P. The clinical significance of circulating tumor cells in non-metastatic colorectal cancer–a review. Eur J Surg Oncol. 2011;37(6):459–465. doi:10.1016/j.ejso.2011.01.02521324632
   PubMed Web of Science ®Google Scholar
• Xu L, Shamash J, Lu Y-J. Circulating tumor cells: a window to understand cancer metastasis, monitor and fight against cancers. J Can Res Updates. 2015;4(1):13–29. doi:10.6000/1929-2279.2015.04.01.2
   Google Scholar
• Satelli A, Mitra A, Brownlee Z, et al. Epithelial–mesenchymal transitioned circulating tumor cells capture for detecting tumor progression. Clin Cancer Res. 2015;21(4):899–906. doi:10.1158/1078-0432.CCR-14-089425516888
   PubMed Web of Science ®Google Scholar
• Cohen SJ, Punt CJ, Iannotti N, et al. Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer. J Clin Oncol. 2008;26(19):3213–3221. doi:10.1200/JCO.2007.15.892318591556
   PubMed Web of Science ®Google Scholar
• Riggi N, Aguet M, Stamenkovic I. Cancer metastasis: a reappraisal of its underlying mechanisms and their relevance to treatment. Annu Rev Pathol. 2018;13:117–140. doi:10.1146/annurev-pathol-020117-04412729068753
   PubMed Web of Science ®Google Scholar
• Pan BT, Johnstone RM. Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: selective externalization of the receptor. Cell. 1983;33(3):967–978. doi:10.1016/0092-8674(83)90040-56307529
   PubMed Web of Science ®Google Scholar
• Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in exosome isolation techniques. Theranostics. 2017;7(3):789–804. doi:10.7150/thno.1813328255367
   PubMed Web of Science ®Google Scholar
• Soung YH, Nguyen T, Cao H, Lee J, Chung J. Emerging roles of exosomes in cancer invasion and metastasis. BMB Rep. 2016;49(1):18–25. doi:10.5483/BMBRep.2016.49.1.23926592936
   PubMed Web of Science ®Google Scholar
• Traci Pawlowski DS, Tinder T, Kimbrough J, et al. Circulating exosomes may provide an alternate platform to monitor disease progression compared to circulating tumor cells. J Clin Oncol 2010;28:10580–10580. doi:10.1200/jco.2010.28.15_suppl.10580
   Google Scholar
• Moher D, Liberati A, Tetzlaff J, Altman DG, Group P. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009;6(7):e1000097. doi:10.1371/journal.pmed.100009719621072
   PubMed Web of Science ®Google Scholar
• Stang A. Critical evaluation of the Newcastle-Ottawa scale for the assessment of the quality of nonrandomized studies in meta-analyses. Eur J Epidemiol. 2010;25(9):603–605. doi:10.1007/s10654-010-9491-z20652370
   PubMed Web of Science ®Google Scholar
• Harris MA, Clark J, Ireland A, et al. The Gene Ontology (GO) database and informatics resource. Nucleic Acids Res. 2004;32(Databaseissue):D258–D261. doi:10.1093/nar/gkh03614681407
   PubMedGoogle Scholar
• Zhang XW, Yang HY, Fan P, Yang L, Chen GY. Detection of micrometastasis in peripheral blood by multi-sampling in patients with colorectal cancer. World J Gastroenterol. 2005;11(3):436–438. doi:10.3748/wjg.v11.i3.43615637763
   PubMed Web of Science ®Google Scholar
• Katsumata K, Sumi T, Mori Y, Hisada M, Tsuchida A, Aoki T. Detection and evaluation of epithelial cells in the blood of colon cancer patients using RT-PCR. Int J Clin Oncol. 2006;11(5):385–389. doi:10.1007/s10147-006-0590-517058136
   PubMedGoogle Scholar
• Soeth E, Vogel I, Roder C, et al. Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR. Cancer Res. 1997;57(15):3106–3110.9242433
   PubMed Web of Science ®Google Scholar
• Soeth E, Roder C, Juhl H, Kruger U, Kremer B, Kalthoff H. The detection of disseminated tumor cells in bone marrow from colorectal-cancer patients by a cytokeratin-20-specific nested reverse-transcriptase-polymerase-chain reaction is related to the stage of disease. Int J Cancer. 1996;69(4):278–282. doi:10.1002/(SICI)1097-0215(19960822)69:4<278::AID-IJC7>3.0.CO;2-U8797868
   PubMed Web of Science ®Google Scholar
• Vlems FA, Diepstra JH, Cornelissen IM, et al. Limitations of cytokeratin 20 RT-PCR to detect disseminated tumour cells in blood and bone marrow of patients with colorectal cancer: expression in controls and downregulation in tumour tissue. Mol Pathol. 2002;55(3):156–163. doi:10.1136/mp.55.3.15612032226
   PubMedGoogle Scholar
• Guo J, Xiao B, Jin Z, et al. Detection of cytokeratin 20 mRNA in the peripheral blood of patients with colorectal cancer by immunomagnetic bead enrichment and real-time reverse transcriptase-polymeras chain reaction. J Gastroenterol Hepatol. 2005;20(8):1279–1284. doi:10.1111/j.1440-1746.2005.03894.x16048578
   PubMed Web of Science ®Google Scholar
• Jonas S, Windeatt S, Fordy C, Allen-Mersh TG. Identification of carcinoembryonic antigen-producing cells circulating in the blood of patients with colorectal carcinoma by reverse transcriptase polymerase chain reaction. Gut. 1996;39(5):717–721. doi:10.1136/gut.39.5.7179014772
   PubMed Web of Science ®Google Scholar
• Castells A, Boix L, Bessa X, Gargallo L, Pique JM. Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction. Br J Cancer. 1998;78(10):1368–1372. doi:10.1038/bjc.1998.6869823981
   PubMed Web of Science ®Google Scholar
• Noh YH, Im G, Ku JH, Lee YS, Ahn MJ. Detection of tumor cell contamination in peripheral blood by RT-PCR in gastrointestinal cancer patients. J Korean Med Sci. 1999;14(6):623–628. doi:10.3346/jkms.1999.14.6.62310642939
   PubMed Web of Science ®Google Scholar
• Guadagni F, Kantor J, Aloe S, et al. Detection of blood-borne cells in colorectal cancer patients by nested reverse transcription-polymerase chain reaction for carcinoembryonic antigen messenger RNA: longitudinal analyses and demonstration of its potential importance as an adjunct to multiple serum markers. Cancer Res. 2001;61(6):2523–2532.11289125
   PubMed Web of Science ®Google Scholar
• Hampton R, Walker M, Marshall J, Juhl H. Differential expression of carcinoembryonic antigen (CEA) splice variants in whole blood of colon cancer patients and healthy volunteers: implication for the detection of circulating colon cancer cells. Oncogene. 2002;21(51):7817–7823. doi:10.1038/sj.onc.120590612420218
   PubMed Web of Science ®Google Scholar
• Guo J, Xiao B, Zhang X, et al. Combined use of positive and negative immunomagnetic isolation followed by real-time RT-PCR for detection of the circulating tumor cells in patients with colorectal cancers. J Mol Med (Berl). 2004;82(11):768–774. doi:10.1007/s00109-004-0590-815490093
   PubMed Web of Science ®Google Scholar
• Silva JM, Rodriguez R, Garcia JM, et al. Detection of epithelial tumour RNA in the plasma of colon cancer patients is associated with advanced stages and circulating tumour cells. Gut. 2002;50(4):530–534. doi:10.1136/gut.50.4.53011889075
   PubMed Web of Science ®Google Scholar
• Wong IH, Yeo W, Chan AT, Johnson PJ. Quantitative relationship of the circulating tumor burden assessed by reverse transcription-polymerase chain reaction for cytokeratin 19 mRNA in peripheral blood of colorectal cancer patients with Dukes’ stage, serum carcinoembryonic antigen level and tumor progression. Cancer Lett. 2001;162(1):65–73. doi:10.1016/s0304-3835(00)00630-311121864
   PubMed Web of Science ®Google Scholar
• Cohen SJ, Alpaugh RK, Gross S, et al. Isolation and characterization of circulating tumor cells in patients with metastatic colorectal cancer. Clin Colorectal Cancer. 2006;6(2):125–132. doi:10.3816/CCC.2006.n.02916945168
   PubMed Web of Science ®Google Scholar
• Gogoi P, Sepehri S, Zhou Y, et al. Development of an automated and sensitive microfluidic device for capturing and characterizing Circulating Tumor Cells (CTCs) from clinical blood samples. PLoS One. 2016;11(1):e0147400. doi:10.1371/journal.pone.014740026808060
   PubMed Web of Science ®Google Scholar
• Mourtzikou A, Kroupis C, Poumpouridou N, et al. Molecular detection of circulating tumor cells in peripheral blood of colon cancer patients. J Gastroenterol Hepatol Res. 2012;1(5):74–79.
   Google Scholar
• van Zijl F, Krupitza G, Mikulits W. Initial steps of metastasis: cell invasion and endothelial transmigration. Mutat Res. 2011;728(1–2):23–34. doi:10.1016/j.mrrev.2011.05.00221605699
   PubMed Web of Science ®Google Scholar
• Nagrath S, Sequist LV, Maheswaran S, et al. Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007;450(7173):1235–1239. doi:10.1038/nature0638518097410
   PubMed Web of Science ®Google Scholar
• Raeisossadati R, Farshchian M, Ganji A, et al. Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients. Int J Colorectal Dis. 2011;26(10):1265–1270. doi:10.1007/s00384-011-1230-821573768
   PubMed Web of Science ®Google Scholar
• Wong LS, Cantrill JE, Odogwu S, Morris AG, Fraser IA. Detection of circulating tumour cells and nodal metastasis by reverse transcriptase-polymerase chain reaction technique. Br J Surg. 1997;84(6):834–839.9189103
   PubMed Web of Science ®Google Scholar
• Fava TA, Desnoyers R, Schulz S, et al. Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. J Clin Oncol. 2001;19(19):3951–3959. doi:10.1200/JCO.2001.19.19.395111579116
   PubMed Web of Science ®Google Scholar
• Tien YW, Chang KJ, Jeng YM, et al. Tumor angiogenesis and its possible role in intravasation of colorectal epithelial cells. Clin Cancer Res. 2001;7(6):1627–1632.11410499
   PubMed Web of Science ®Google Scholar
• Tien YW, Jeng YM, Hu RH, Chang KJ, Hsu SM, Lee PH. Intravasation-related metastatic factors in colorectal cancer. Tumour Biol. 2004;25(1–2):48–55. doi:10.1159/00007772315192312
   PubMedGoogle Scholar
• Solmi R, De Sanctis P, Zucchini C, et al. Search for epithelial-specific mRNAs in peripheral blood of patients with colon cancer by RT-PCR. Int J Oncol. 2004;25(4):1049–1056. doi:10.3892/ijo.25.4.104915375555
   PubMed Web of Science ®Google Scholar
• Yeh CS, Wang JY, Wu CH, et al. Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel. Int J Oncol. 2006;28(2):411–420. doi:10.3892/ijo.28.2.41116391796
   PubMed Web of Science ®Google Scholar
• Solmi R, Ugolini G, Rosati G, et al. Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer. BMC Cancer. 2006;6:250. doi:10.1186/1471-2407-6-25017054783
   PubMed Web of Science ®Google Scholar
• Lauriola M, Ugolini G, Rosati G, et al. Identification by a Digital Gene Expression Displayer (DGED) and test by RT-PCR analysis of new mRNA candidate markers for colorectal cancer in peripheral blood. Int J Oncol. 2010;37(2):519–525. doi:10.3892/ijo_0000070120596680
   PubMed Web of Science ®Google Scholar
• Barbazan J, Alonso-Alconada L, Muinelo-Romay L, et al. Molecular characterization of circulating tumor cells in human metastatic colorectal cancer. PLoS One. 2012;7(7):e40476. doi:10.1371/journal.pone.004047622811761
   PubMed Web of Science ®Google Scholar
• Barbazan J, Vieito M, Abalo A, et al. A logistic model for the detection of circulating tumour cells in human metastatic colorectal cancer. J Cell Mol Med. 2012;16(10):2342–2349. doi:10.1111/j.1582-4934.2012.01544.x22304365
   PubMed Web of Science ®Google Scholar
• Alonso-Alconada L, Barbazan J, Candamio S, et al. PrediCTC, liquid biopsy in precision oncology: a technology transfer experience in the Spanish health system. Clin Transl Oncol. 2018;20(5):630–638. doi:10.1007/s12094-017-1760-929058262
   PubMed Web of Science ®Google Scholar
• Barbazan J, Muinelo-Romay L, Vieito M, et al. A multimarker panel for circulating tumor cells detection predicts patient outcome and therapy response in metastatic colorectal cancer. Int J Cancer. 2014;135(11):2633–2643. doi:10.1002/ijc.2891024752533
   PubMed Web of Science ®Google Scholar
• Findeisen P, Rockel M, Nees M, et al. Systematic identification and validation of candidate genes for detection of circulating tumor cells in peripheral blood specimens of colorectal cancer patients. Int J Oncol. 2008;33(5):1001–1010. doi:10.3892/ijo_0000008818949363
   PubMed Web of Science ®Google Scholar
• Vaiopoulos AG, Kostakis ID, Gkioka E, et al. Detection of circulating tumor cells in colorectal and gastric cancer using a multiplex PCR assay. Anticancer Res. 2014;34(6):3083–3092.24922677
   PubMed Web of Science ®Google Scholar
• Teama SH, Agwa SHA. Detection of circulating tumor cells by nested RT-PCR targeting EGFR/CEA/CK20mRNAs in colorectal carcinoma patients. Egypt J Med Human Genet. 2010;11(2):173–180. doi:10.1016/j.ejmhg.2009.10.001
   Google Scholar
• Lankiewicz S, Rother E, Zimmermann S, Hollmann C, Korangy F, Greten TF. Tumour-associated transcripts and EGFR deletion variants in colorectal cancer in primary tumour, metastases and circulating tumour cells. Cell Oncol. 2008;30(6):463–471.18936523
   PubMed Web of Science ®Google Scholar
• Chen CJ, Sung WW, Chen HC, et al. Early assessment of colorectal cancer by quantifying circulating tumor cells in peripheral blood: ECT2 in diagnosis of colorectal cancer. Int J Mol Sci. 2017;18(4):E743. doi:10.3390/ijms1804074328362321
   PubMed Web of Science ®Google Scholar
• Cui CH, Chen RH, Zhai DY, Xie L, Qi J, Yu JL. Detection of FAM172A expressed in circulating tumor cells is a feasible method to predict high-risk subgroups of colorectal cancer. Tumour Biol. 2017;39(6):1010428317699126. doi:10.1177/101042831769912628618931
   PubMedGoogle Scholar
• Gessi S, Cattabriga E, Avitabile A, et al. Elevated expression of A3 adenosine receptors in human colorectal cancer is reflected in peripheral blood cells. Clin Cancer Res. 2004;10(17):5895–5901. doi:10.1158/1078-0432.CCR-1134-0315355922
   PubMed Web of Science ®Google Scholar
• Rodia MT, Ugolini G, Mattei G, et al. Systematic large-scale meta-analysis identifies a panel of two mRNAs as blood biomarkers for colorectal cancer detection. Oncotarget. 2016;7(21):30295–30306. doi:10.18632/oncotarget.810826993598
   PubMedGoogle Scholar
• Ogata-Kawata H, Izumiya M, Kurioka D, et al. Circulating exosomal microRNAs as biomarkers of colon cancer. PLoS One. 2014;9(4). doi:10.1371/journal.pone.0092921
   PubMed Web of Science ®Google Scholar
• Zhang J, Raju GS, Chang DW, Lin SH, Chen Z, Wu X. Global and targeted circulating microRNA profiling of colorectal adenoma and colorectal cancer. Cancer. 2018;124(4):785–796. doi:10.1002/cncr.3106229112225
   PubMed Web of Science ®Google Scholar
• Ostenfeld MS, Jensen SG, Jeppesen DK, et al. miRNA profiling of circulating EpCAM+ extracellular vesicles: promising biomarkers of colorectal cancer. J Extracell Vesicles. 2016;5(1). doi:10.3402/jev.v5.31488
   PubMedGoogle Scholar
• Rapado-Gonzalez O, Alvarez-Castro A, Lopez-Lopez R, Iglesias-Canle J, Suarez-Cunqueiro MM, Muinelo-Romay L. Circulating microRNAs as promising biomarkers in colorectal cancer. Cancers. 2019;11(7). doi:10.3390/cancers11070898
   PubMed Web of Science ®Google Scholar
• Hao YX, Li YM, Ye M, et al. KRAS and BRAF mutations in serum exosomes from patients with colorectal cancer in a Chinese population. Oncol Lett. 2017;13(5):3608–3616. doi:10.3892/ol.2017.588928521461
   PubMed Web of Science ®Google Scholar
• Soldevilla B, Rodriguez M, Millán CS, et al. Tumor-derived exosomes are enriched in ΔNp73, which promotes oncogenic potential in acceptor cells and correlates with patient survival. Hum Mol Genet. 2014;23(2):467–478. doi:10.1093/hmg/ddt43724067531
   PubMed Web of Science ®Google Scholar
• Chiba M, Kimura M, Asari S. Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines. Oncol Rep. 2012;28(5):1551–1558. doi:10.3892/or.2012.196722895844
   PubMed Web of Science ®Google Scholar
• Barbagallo C, Brex D, Caponnetto A, et al. LncRNA UCA1, upregulated in CRC biopsies and downregulated in serum exosomes, controls mRNA expression by RNA-RNA interactions. Mol Ther. 2018;12:229–241. doi:10.1016/j.omtn.2018.05.009
   Web of Science ®Google Scholar
• Li Y, Zhao J, Yu S, et al. Extracellular vesicles long RNA sequencing reveals abundant mRNA, circRNA, and lncRNA in human blood as potential biomarkers for cancer diagnosis. Clin Chem. 2019;65(6):798–808. doi:10.1373/clinchem.2018.30129130914410
   PubMed Web of Science ®Google Scholar
• Wang L, Duan W, Yan S, Xie Y, Wang C. Circulating long non-coding RNA colon cancer-associated transcript 2 protected by exosome as a potential biomarker for colorectal cancer. Biomed Pharmacother. 2019;113. doi:10.1016/j.biopha.2019.108758
   Web of Science ®Google Scholar
• Liu H, Ye D, Chen A, et al. A pilot study of new promising non-coding RNA diagnostic biomarkers for early-stage colorectal cancers. Clin Chem Lab Med. 2019;57(7):1073–1083. doi:10.1515/cclm-2019-005230978169
   PubMed Web of Science ®Google Scholar
• Nakai W, Yoshida T, Diez D, et al. A novel affinity-based method for the isolation of highly purified extracellular vesicles. Sci Rep. 2016;6:33935. doi:10.1038/srep3393527659060
   PubMed Web of Science ®Google Scholar
• Manri C, Yokoi T, Nishida H. Size-selective harvesting of extracellular vesicles for strategic analyses towards tumor diagnoses. Appl Biochem Biotechnol. 2017;182(2):609–623. doi:10.1007/s12010-016-2348-527917441
   PubMed Web of Science ®Google Scholar
• Li J, Chen Y, Guo X, et al. GPC1 exosome and its regulatory miRNAs are specific markers for the detection and target therapy of colorectal cancer. J Cell Mol Med. 2017;21(5):838–847. doi:10.1111/jcmm.1294128233416
   PubMed Web of Science ®Google Scholar
• Dong L, Lin W, Qi P, et al. Circulating long RNAs in serum extracellular vesicles: their characterization and potential application as biomarkers for diagnosis of colorectal cancer. Cancer Epidemiol Biomarkers Prev. 2016;25(7):1158–1166. doi:10.1158/1055-9965.EPI-16-000627197301
   PubMed Web of Science ®Google Scholar
• Morio H, Sun Y, Harada M, et al. Cancer-Type OATP1B3 mRNA in extracellular vesicles as a promising candidate for a serum-based colorectal cancer biomarker. Biol Pharm Bull. 2018;41(3):445–449. doi:10.1248/bpb.b17-0074329491222
   PubMed Web of Science ®Google Scholar
• Huang Q, Shen Z, Zang R, Fan X, Yang L, Xue M. Identification of novel genes and pathways in colorectal cancer exosomes: a bioinformatics study. Transl Cancer Res. 2018;7(3):651–658. doi:10.21037/tcr
   Web of Science ®Google Scholar
• Wyld DK, Selby P, Perren TJ, et al. Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytokeratin 20. Int J Cancer. 1998;79(3):288–293. doi:10.1002/(sici)1097-0215(19980619)79:3<288::aid-ijc14>3.0.co;2-49645353
   PubMed Web of Science ®Google Scholar
• Hinz S, Bockhorst J, Roder C, et al. Disseminated tumor cells in the bone marrow negatively influence survival after resection of colorectal liver metastases. Ann Surg Oncol. 2012;19(8):2539–2546. doi:10.1245/s10434-012-2291-922395998
   PubMed Web of Science ®Google Scholar
• Funaki NO, Tanaka J, Itami A, et al. Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA. Life Sci. 1997;60(9):643–652. doi:10.1016/s0024-3205(96)00700-x9048967
   PubMed Web of Science ®Google Scholar
• Samija I, Lukac J, Mubrin MK, Kirac I, Kovacevic D, Kusic Z. Detection of cytokeratin-20-positive cells in preoperative and postoperative blood samples from colorectal cancer patients by real-time RT-PCR. Int J Biol Markers. 2013;28(2):174–181. doi:10.5301/jbm.500000323558939
   PubMed Web of Science ®Google Scholar
• Kust D, Samija I, Kirac I, Radic J, Kovacevic D, Kusic Z. Cytokeratin 20 positive cells in blood of colorectal cancer patients as an unfavorable prognostic marker. Acta Clin Belg. 2016;71(4):235–243. doi:10.1080/17843286.2016.117726427144776
   PubMed Web of Science ®Google Scholar
• Piva MG, Navaglia F, Basso D, et al. CEA mRNA identification in peripheral blood is feasible for colorectal, but not for gastric or pancreatic cancer staging. Oncology. 2000;59(4):323–328. doi:10.1159/00001219011096345
   PubMed Web of Science ®Google Scholar
• Ito S, Nakanishi H, Hirai T, et al. Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with real-time RT-PCR on a LightCycler. Cancer Lett. 2002;183(2):195–203. doi:10.1016/s0304-3835(02)00157-x12065095
   PubMed Web of Science ®Google Scholar
• Kanellos I, Zacharakis E, Kanellos D, et al. Prognostic significance of CEA levels and detection of CEA mRNA in draining venous blood in patients with colorectal cancer. J Surg Oncol. 2006;94(1):3–8. doi:10.1002/jso.2054916788936
   PubMed Web of Science ®Google Scholar
• Lu CY, Uen YH, Tsai HL, et al. Molecular detection of persistent postoperative circulating tumour cells in stages II and III colon cancer patients via multiple blood sampling: prognostic significance of detection for early relapse. Br J Cancer. 2011;104(7):1178–1184. doi:10.1038/bjc.2011.4021343933
   PubMed Web of Science ®Google Scholar
• Thorsteinsson M, Soletormos G, Jess P. Low number of detectable circulating tumor cells in non-metastatic colon cancer. Anticancer Res. 2011;31(2):613–617.21378346
   PubMed Web of Science ®Google Scholar
• Yamaguchi K, Takagi Y, Aoki S, Futamura M, Saji S. Significant detection of circulating cancer cells in the blood by reverse transcriptase-polymerase chain reaction during colorectal cancer resection. Ann Surg. 2000;232(1):58–65. doi:10.1097/00000658-200007000-0000910862196
   PubMed Web of Science ®Google Scholar
• Mathur P, Wharton RQ, Jonas SK, Saini S, Allen-Mersh TG. Relationship between tumour vascularity and circulating cancer cells in patients with colorectal carcinoma. Eur J Surg Oncol. 2001;27(4):354–358. doi:10.1053/ejso.2001.111811417979
   PubMedGoogle Scholar
• Guller U, Zajac P, Schnider A, et al. Disseminated single tumor cells as detected by real-time quantitative polymerase chain reaction represent a prognostic factor in patients undergoing surgery for colorectal cancer. Ann Surg. 2002;236(6):768–775; discussion 775–766. doi:10.1097/00000658-200212000-00009
   Web of Science ®Google Scholar
• Iinuma H, Okinaga K, Egami H, et al. Usefulness and clinical significance of quantitative real-time RT-PCR to detect isolated tumor cells in the peripheral blood and tumor drainage blood of patients with colorectal cancer. Int J Oncol. 2006;28(2):297–306. doi:10.3892/ijo.28.2.29716391782
   PubMed Web of Science ®Google Scholar
• Liu ZP, Li LM, Liu XL, Zhang DX. Comparative analysis of tumor markers and evaluation of their predictive value in patients with colorectal cancer. Onkologie. 2012;35(3):108–113. doi:10.1159/00033681622414974
   PubMedGoogle Scholar
• Huang P, Wang J, Guo Y, Xie W. Molecular detection of disseminated tumor cells in the peripheral blood in patients with gastrointestinal cancer. J Cancer Res Clin Oncol. 2003;129(3):192–198. doi:10.1007/s00432-003-0425-y12684893
   PubMed Web of Science ®Google Scholar
• Nakamori S, Kameyama M, Furukawa H, et al. Genetic detection of colorectal cancer cells in circulation and lymph nodes. Dis Colon Rectum. 1997;40(10 Suppl):S29–S36. doi:10.1007/bf020620179378009
   PubMed Web of Science ®Google Scholar
• Hardingham JE, Hewett PJ, Sage RE, et al. Molecular detection of blood-borne epithelial cells in colorectal cancer patients and in patients with benign bowel disease. Int J Cancer. 2000;89(1):8–13. doi:10.1002/(SICI)1097-021510719724
   PubMed Web of Science ®Google Scholar
• Shimada R, Iinuma H, Akahane T, Horiuchi A, Watanabe T. Prognostic significance of CTCs and CSCs of tumor drainage vein blood in Dukes’ stage B and C colorectal cancer patients. Oncol Rep. 2012;27(4):947–953. doi:10.3892/or.2012.164922267181
   PubMed Web of Science ®Google Scholar
• Xi L, Nicastri DG, El-Hefnawy T, Hughes SJ, Luketich JD, Godfrey TE. Optimal markers for real-time quantitative reverse transcription PCR detection of circulating tumor cells from melanoma, breast, colon, esophageal, head and neck, and lung cancers. Clin Chem. 2007;53(7):1206–1215. doi:10.1373/clinchem.2006.08182817525108
   PubMed Web of Science ®Google Scholar
• Winter M, Cai Z, Winkler K, et al. Circulating tumour cell RNA characterisation from colorectal cancer patient blood after inertial microfluidic enrichment. MethodsX. 2019;6:1512–1520. doi:10.1016/j.mex.2019.06.01231304099
   PubMed Web of Science ®Google Scholar
• Liu Y, Cheng G, Qian J, et al. Expression of guanylyl cyclase C in tissue samples and the circulation of rectal cancer patients. Oncotarget. 2017;8(24):38841–38849. doi:10.18632/oncotarget.1640628418917
   PubMedGoogle Scholar
• Liu Y, Qian J, Feng JG, et al. Detection of circulating tumor cells in peripheral blood of colorectal cancer patients without distant organ metastases. Cell Oncol. 2013;36(1):43–53. doi:10.1007/s13402-012-0112-6
   Web of Science ®Google Scholar
• Chalopin A, Tellez-Gabriel M, Brown HK, et al. Isolation of circulating tumor cells in a preclinical model of osteosarcoma: effect of chemotherapy. J Bone Oncol. 2018;12:83–90. doi:10.1016/j.jbo.2018.07.00230123735
   PubMed Web of Science ®Google Scholar
• Iinuma H, Watanabe T, Mimori K, et al. Clinical significance of circulating tumor cells, including cancer stem-like cells, in peripheral blood for recurrence and prognosis in patients with Dukes’ stage B and C colorectal cancer. J Clin Oncol. 2011;29(12):1547–1555. doi:10.1200/JCO.2010.30.515121422427
   PubMed Web of Science ®Google Scholar
• Zieglschmid V, Hollmann C, Mannel J, et al. Tumor-associated gene expression in disseminated tumor cells correlates with disease progression and tumor stage in colorectal cancer. Anticancer Res. 2007;27(4a):1823–1832.17649779
   PubMed Web of Science ®Google Scholar
• Sato N, Hayashi N, Imamura Y, et al. Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer. Ann Surg Oncol. 2012;19(6):2060–2065. doi:10.1245/s10434-011-1889-721732137
   PubMed Web of Science ®Google Scholar
• Mirzaei A, Tavoosidana G, Rad AA, et al. A new insight into cancer stem cell markers: could local and circulating cancer stem cell markers correlate in colorectal cancer? Tumor Biol. 2016;37(2):2405–2414. doi:10.1007/s13277-015-3989-7
   PubMedGoogle Scholar
• Wu S, Liu S, Liu Z, et al. Classification of circulating tumor cells by epithelial-mesenchymal transition markers. PLoS One. 2015;10(4):e0123976. doi:10.1371/journal.pone.012397625909322
   PubMed Web of Science ®Google Scholar
• Shen C, Hu L, Xia L, Li Y. Quantitative real-time RT-PCR detection for survivin, CK20 and CEA in peripheral blood of colorectal cancer patients. Jpn J Clin Oncol. 2008;38(11):770–776. doi:10.1093/jjco/hyn10518845519
   PubMed Web of Science ®Google Scholar
• Schuster R, Max N, Mann B, et al. Quantitative real-time RT-PCR for detection of disseminated tumor cells in peripheral blood of patients with colorectal cancer using different mRNA markers. Int J Cancer. 2004;108(2):219–227. doi:10.1002/ijc.1154714639606
   PubMed Web of Science ®Google Scholar
• Wang JY, Wu CH, Lu CY, et al. Molecular detection of circulating tumor cells in the peripheral blood of patients with colorectal cancer using RT-PCR: significance of the prediction of postoperative metastasis. World J Surg. 2006;30(6):1007–1013. doi:10.1007/s00268-005-0485-z16736329
   PubMed Web of Science ®Google Scholar
• Wang JY, Lin SR, Wu DC, et al. Multiple molecular markers as predictors of colorectal cancer in patients with normal perioperative serum carcinoembryonic antigen levels. Clin Cancer Res. 2007;13(8):2406–2413. doi:10.1158/1078-0432.CCR-06-205417406027
   PubMed Web of Science ®Google Scholar
• Douard R, Moutereau S, Serru V, et al. Immunobead multiplex RT-PCR detection of carcinoembryonic genes expressing cells in the blood of colorectal cancer patients. Clin Chem Lab Med. 2005;43(2):127–132. doi:10.1515/CCLM.2005.02115843204
   PubMed Web of Science ®Google Scholar
• Douard R, Le Maire V, Wind P, et al. Carcinoembryonic gene member 2 mRNA expression as a marker to detect circulating enterocytes in the blood of colorectal cancer patients. Surgery. 2001;129(5):587–594. doi:10.1067/msy.2001.11248511331451
   PubMed Web of Science ®Google Scholar
• Bessa X, Elizalde JI, Boix L, et al. Lack of prognostic influence of circulating tumor cells in peripheral blood of patients with colorectal cancer. Gastroenterology. 2001;120(5):1084–1092. doi:10.1053/gast.2001.2324511266372
   PubMed Web of Science ®Google Scholar
• De Luca A, Pignata S, Casamassimi A, et al. Detection of circulating tumor cells in carcinoma patients by a novel epidermal growth factor receptor reverse transcription-PCR assay. Clin Cancer Res. 2000;6(4):1439–1444.10778975
   PubMed Web of Science ®Google Scholar
• Clarke LE, Leitzel K, Smith J, Ali SM, Lipton A. Epidermal growth factor receptor mRNA in peripheral blood of patients with pancreatic, lung, and colon carcinomas detected by RT-PCR. Int J Oncol. 2003;22(2):425–430. doi:10.3892/ijo.22.2.42512527944
   PubMed Web of Science ®Google Scholar
• Fournier MV, Guimaraes Da Costa F, Paschoal ME, Ronco LV, Carvalho MG, Pardee AB. Identification of a gene encoding a human oxysterol-binding protein-homologue: a potential general molecular marker for blood dissemination of solid tumors. Cancer Res. 1999;59(15):3748–3753.10446991
   PubMed Web of Science ®Google Scholar
• Gazzaniga P, Nofroni I, Gandini O, et al. Tenascin C and epidermal growth factor receptor as markers of circulating tumoral cells in bladder and colon cancer. Oncol Rep. 2005;14(5):1199–1202. doi:10.3892/or.14.5.119916211285
   PubMed Web of Science ®Google Scholar
• Yokobori T, Iinuma H, Shimamura T, et al. Plastin3 is a novel marker for circulating tumor cells undergoing the epithelial-mesenchymal transition and is associated with colorectal cancer prognosis. Cancer Res. 2013;73(7):2059–2069. doi:10.1158/0008-5472.CAN-12-032623378342
   PubMed Web of Science ®Google Scholar
• Sugimachi K, Yokobori T, Iinuma H, et al. Aberrant expression of plastin-3 via copy number gain induces the epithelial-mesenchymal transition in circulating colorectal cancer cells. Ann Surg Oncol. 2014;21(11):3680–3690. doi:10.1245/s10434-013-3366-y24217791
   PubMed Web of Science ®Google Scholar
• Valladares-Ayerbes M, Díaz-Prado S, Reboredo M, et al. Bioinformatics approach to mRNA markers discovery for detection of circulating tumor cells in patients with gastrointestinal cancer. Cancer Detect Prev. 2008;32(3):236–250. doi:10.1016/j.cdp.2008.08.00218801625
   PubMedGoogle Scholar
• Mostert B, Sieuwerts AM, Bolt-de Vries J, et al. mRNA expression profiles in circulating tumor cells of metastatic colorectal cancer patients. Mol Oncol. 2015;9(4):920–932. doi:10.1016/j.molonc.2015.01.00125655581
   PubMed Web of Science ®Google Scholar
• Valladares-Ayerbes M, Blanco-Calvo M, Reboredo M, et al. Evaluation of the adenocarcinoma-associated gene AGR2 and the intestinal stem cell marker LGR5 as biomarkers in colorectal cancer. Int J Mol Sci. 2012;13(4):4367–4387. doi:10.3390/ijms1304436722605983
   PubMed Web of Science ®Google Scholar
• Mirzaei A, Tavoosidana G, Modarressi MH, et al. Upregulation of circulating cancer stem cell marker, DCLK1 but not Lgr5, in chemoradiotherapy-treated colorectal cancer patients. Tumour Biol. 2015;36(6):4801–4810. doi:10.1007/s13277-015-3132-925631749
   PubMedGoogle Scholar
• Wang W, Wan L, Wu S, et al. Mesenchymal marker and LGR5 expression levels in circulating tumor cells correlate with colorectal cancer prognosis. Cell Oncol. 2018;45:1–10. doi:10.1007/s13402-018-0386-4
   Google Scholar
• Ning Y, Zhang W, Hanna DL, et al. Clinical relevance of EMT and stem-like gene expression in circulating tumor cells of metastatic colorectal cancer patients. Pharmacogenomics J. 2018;18(1):29–34. doi:10.1038/tpj.2016.6227503579
   PubMed Web of Science ®Google Scholar
• Gradilone A, Gazzaniga P, Silvestri I, et al. Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: correlation with clinical stage of disease. Oncol Rep. 2003;10(1):217–222. doi:10.3892/or.10.1.21712469172
   PubMed Web of Science ®Google Scholar
• Shan T, Cui X, Li W, Lin W, Li Y. AQP5: a novel biomarker that predicts poor clinical outcome in colorectal cancer. Oncol Rep. 2014;32(4):1564–1570. doi:10.3892/or.2014.337725109507
   PubMed Web of Science ®Google Scholar
• Burchill SA, Perebolte L, Johnston C, Top B, Selby P. Comparison of the RNA-amplification based methods RT-PCR and NASBA for the detection of circulating tumour cells. Br J Cancer. 2002;86(1):102–109. doi:10.1038/sj.bjc.660001411857020
   PubMed Web of Science ®Google Scholar
• Miyashiro I, Kuo C, Huynh K, et al. Molecular strategy for detecting metastatic cancers with use of multiple tumor-specific MAGE-A genes. Clin Chem. 2001;47(3):505–512.11238304
   PubMed Web of Science ®Google Scholar
• Huang MY, Yen LC, Liu HC, et al. Significant overexpression of DVL1 in Taiwanese colorectal cancer patients with liver metastasis. Int J Mol Sci. 2013;14(10):20492–20507. doi:10.3390/ijms14102049224129181
   PubMed Web of Science ®Google Scholar
• Steinert G, Scholch S, Niemietz T, et al. Immune escape and survival mechanisms in circulating tumor cells of colorectal cancer. Cancer Res. 2014;74(6):1694–1704. doi:10.1158/0008-5472.CAN-13-188524599131
   PubMed Web of Science ®Google Scholar
• Katoh S, Goi T, Naruse T, et al. Cancer stem cell marker in circulating tumor cells: expression of CD44 variant exon 9 is strongly correlated to treatment refractoriness, recurrence and prognosis of human colorectal cancer. Anticancer Res. 2015;35(1):239–244.25550556
   PubMed Web of Science ®Google Scholar
• Bao H, Burke PA, Huang J, et al. Circulating tumor cells: application as a biomarker for molecular characterization and predictor of survival in an all-comer solid tumor phase i clinical study. PLoS One. 2013;8(8):e58557. doi:10.1371/journal.pone.005855723990866
   PubMed Web of Science ®Google Scholar
• Chang YT, Huang MY, Yeh YS, et al. A prospective study of comparing multi-gene biomarker chip and serum carcinoembryonic antigen in the postoperative surveillance for patients with stage I-III colorectal cancer. PLoS One. 2016;11(10):e0163264. doi:10.1371/journal.pone.016326427701415
   PubMed Web of Science ®Google Scholar
• Bahnassy AA, Salem SE, Mohanad M, et al. Prognostic significance of circulating tumor cells (CTCs) in Egyptian non-metastatic colorectal cancer patients: A comparative study for four different techniques of detection (Flowcytometry, CellSearch, Quantitative Real-time PCR and Cytomorphology). Exp Mol Pathol. 2019;106:90–101. doi:10.1016/j.yexmp.2018.12.00630578762
   PubMed Web of Science ®Google Scholar
• Valcz G, Galamb O, Krenacs T, et al. Exosomes in colorectal carcinoma formation: ALIX under the magnifying glass. Mod Pathol. 2016;29(8):928–938. doi:10.1038/modpathol.2016.7227150162
   PubMed Web of Science ®Google Scholar
• Tauro BJ, Greening DW, Mathias RA, et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods. 2012;56(2):293–304. doi:10.1016/j.ymeth.2012.01.00222285593
   PubMed Web of Science ®Google Scholar
• Silva J, Garcia V, Rodriguez M, et al. Analysis of exosome release and its prognostic value in human colorectal cancer. Genes Chromosomes Cancer. 2012;51(4):409–418. doi:10.1002/gcc.2192622420032
   PubMed Web of Science ®Google Scholar
• Campanella C, Rappa F, Sciumè C, et al. Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery. Cancer. 2015;121(18):3230–3239. doi:10.1002/cncr.2949926060090
   PubMed Web of Science ®Google Scholar
• Namba Y, Sogawa C, Okusha Y, et al. Depletion of lipid efflux pump ABCG1 triggers the intracellular accumulation of extracellular vesicles and reduces aggregation and tumorigenesis of metastatic cancer cells. Front Oncol. 2018;8:376. doi:10.3389/fonc.2018.0037630364132
   PubMed Web of Science ®Google Scholar
• Sun B, Li Y, Zhou Y, et al. Circulating exosomal CPNE3 as a diagnostic and prognostic biomarker for colorectal cancer. J Cell Physiol. 2019;234(2):1416–1425. doi:10.1002/jcp.2693630078189
   PubMed Web of Science ®Google Scholar
• Chen MS, Xu R, Ji H, et al. Transcriptome and long noncoding RNA sequencing of three extracellular vesicle subtypes released from the human colon cancer LIM1863 cell line. Sci Rep. 2016;6:38397. doi:10.1038/srep3839727917920
   PubMed Web of Science ®Google Scholar
• Galon J, Fridman WH, Pages F. The adaptive immunologic microenvironment in colorectal cancer: a novel perspective. Cancer Res. 2007;67(5):1883–1886. doi:10.1158/0008-5472.CAN-06-480617332313
   PubMed Web of Science ®Google Scholar
• Peddareddigari VG, Wang D, Dubois RN. The tumor microenvironment in colorectal carcinogenesis. Cancer Microenviron. 2010;3(1):149–166. doi:10.1007/s12307-010-0038-321209781
   PubMedGoogle Scholar
• Fu Q, Zhang Q, Lou Y, et al. Primary tumor-derived exosomes facilitate metastasis by regulating adhesion of circulating tumor cells via SMAD3 in liver cancer. Oncogene. 2018;37:6105–6118. doi:10.1038/s41388-018-0391-029991801
   PubMed Web of Science ®Google Scholar
• Crowley E, Di Nicolantonio F, Loupakis F, Bardelli A. Liquid biopsy: monitoring cancer-genetics in the blood. Nat Rev Clin Oncol. 2013;10(8):472–484. doi:10.1038/nrclinonc.2013.11023836314
   PubMed Web of Science ®Google Scholar
• Beauchemin N, Arabzadeh A. Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in cancer progression and metastasis. Cancer Metastasis Rev. 2013;32(3–4):643–671. doi:10.1007/s10555-013-9444-623903773
   PubMed Web of Science ®Google Scholar
• Gold P, Freedman SO. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965;122(3):467–481. doi:10.1084/jem.122.3.4674953873
   PubMed Web of Science ®Google Scholar
• Thomas P, Toth CA, Saini KS, Jessup JM, Steele G Jr. The structure, metabolism and function of the carcinoembryonic antigen gene family. Biochim Biophys Acta. 1990;1032(2–3):177–189. doi:10.1016/0304-419x(90)90003-j2261493
   PubMedGoogle Scholar
• Oikawa S, Inuzuka C, Kuroki M, Matsuoka Y, Kosaki G, Nakazato H. Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: homophilic and heterophilic adhesion. Biochem Biophys Res Commun. 1989;164(1):39–45. doi:10.1016/0006-291x(89)91679-32803308
   PubMed Web of Science ®Google Scholar
• Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin Cancer Biol. 1999;9(2):67–81. doi:10.1006/scbi.1998.011910202129
   PubMed Web of Science ®Google Scholar
• Blumenthal RD, Hansen HJ, Goldenberg DM. Inhibition of adhesion, invasion, and metastasis by antibodies targeting CEACAM6 (NCA-90) and CEACAM5 (Carcinoembryonic Antigen). Cancer Res. 2005;65(19):8809–8817. doi:10.1158/0008-5472.CAN-05-042016204051
   PubMed Web of Science ®Google Scholar
• Ordonez C, Screaton RA, Ilantzis C, Stanners CP. Human carcinoembryonic antigen functions as a general inhibitor of anoikis. Cancer Res. 2000;60(13):3419–3424.10910050
   PubMed Web of Science ®Google Scholar
• Duxbury MS, Ito H, Zinner MJ, Ashley SW, Whang EE. CEACAM6 gene silencing impairs anoikis resistance and in vivo metastatic ability of pancreatic adenocarcinoma cells. Oncogene. 2004;23(2):465–473. doi:10.1038/sj.onc.120703614724575
   PubMed Web of Science ®Google Scholar
• Duffy MJ. Carcinoembryonic antigen as a marker for colorectal cancer: is it clinically useful? Clin Chem. 2001;47(4):624–630.11274010
   PubMed Web of Science ®Google Scholar
• Kim KJ, Kwon HJ, Kim MC, Bae YK. CD9 expression in colorectal carcinomas and its prognostic significance. J Pathol Transl Med. 2016;50(6):459–468. doi:10.4132/jptm.2016.10.0227780340
   PubMedGoogle Scholar
• Mokhtari M, Zakerzade Z. EPCAM expression in colon adenocarcinoma and its relationship with TNM staging. Adv Biomed Res. 2017;6:56. doi:10.4103/2277-9175.20552928553629
   PubMedGoogle Scholar
• Han S, Zong S, Shi Q, et al. Is Ep-CAM expression a diagnostic and prognostic biomarker for colorectal cancer? A systematic meta-analysis. EBioMedicine. 2017;20:61–69. doi:10.1016/j.ebiom.2017.05.02528558958
   PubMed Web of Science ®Google Scholar
• Tan CR, Zhou L, El-Deiry WS. Circulating tumor cells versus circulating tumor DNA in colorectal cancer: pros and cons. Curr Colorectal Cancer Rep. 2016;12(3):151–161. doi:10.1007/s11888-016-0320-y27516729
   PubMed Web of Science ®Google Scholar
• Mathai RAVR, Reddy BS, Thomas L, Udupa K, Kolesar J, Rao M. Potential utility of liquid biopsy as a diagnostic and prognostic tool for the assessment of solid tumors: implications in the precision oncology. J Clin Med. 2019;18(8):3.  doi:10.3390/jcm8030373
   Google Scholar
• Su W, Yu H, Jiang L, Chen W, Li H, Qin J. Integrated microfluidic device for enrichment and identification of circulating tumor cells from the blood of patients with colorectal cancer. Dis Markers. 2019;2019:8945974. doi:10.1155/2019/894597431354892
   PubMed Web of Science ®Google Scholar
• Willms A, Muller C, Julich H, et al. Tumour-associated circulating microparticles: a novel liquid biopsy tool for screening and therapy monitoring of colorectal carcinoma and other epithelial neoplasia. Oncotarget. 2016;7(21):30867–30875. doi:10.18632/oncotarget.901827127176
   PubMedGoogle Scholar