308
Views
9
CrossRef citations to date
0
Altmetric
Original Research

Identification of Hub Genes as Potential Prognostic Biomarkers in Cervical Cancer Using Comprehensive Bioinformatics Analysis and Validation Studies

, , , &
Pages 117-131 | Published online: 08 Jan 2021

References

  • Cohen PA, Jhingran A, Oaknin A, Denny L. Cervical cancer. Lancet. 2019;393(10167):169–182. doi:10.1016/S0140-6736(18)32470-X30638582
  • Abbas KM, van Zandvoort K, Brisson M, Jit M. Effects of updated demography, disability weights, and cervical cancer burden on estimates of human papillomavirus vaccination impact at the global, regional, and national levels: a PRIME modelling study. Lancet Global Health. 2020;8(4):e536–e544. doi:10.1016/S2214-109X(20)30022-X32105613
  • Canfell K, Kim JJ, Brisson M, et al. Mortality impact of achieving WHO cervical cancer elimination targets: a comparative modelling analysis in 78 low-income and lower-middle-income countries. Lancet. 2020;395(10224):591–603. doi:10.1016/S0140-6736(20)30157-432007142
  • The L. Eliminating cervical cancer. Lancet. 2020;395(10221):312. doi:10.1016/S0140-6736(20)30247-6
  • Ward ZJ, Grover S, Scott AM, et al. The role and contribution of treatment and imaging modalities in global cervical cancer management: survival estimates from a simulation-based analysis. Lancet Oncol. 2020;21(8):1089–1098. doi:10.1016/S1470-2045(20)30316-832758463
  • Huang X, Liu S, Wu L, Jiang M, Hou Y. High throughput single cell RNA sequencing, bioinformatics analysis and applications. Adv Exp Med Biol. 2018;1068:33–43.29943294
  • Merrick BA, London RE, Bushel PR, Grissom SF, Paules RS. Platforms for biomarker analysis using high-throughput approaches in genomics, transcriptomics, proteomics, metabolomics, and bioinformatics. IARC Sci Publ. 2011;163:121–142.
  • Tomczak K, Czerwińska P, Wiznerowicz M. The Cancer Genome Atlas (TCGA): an immeasurable source of knowledge. Contemporary Oncol. 2015;19(1a):A68–77.
  • Wang Z, Lachmann A, Ma’ayan A. Mining data and metadata from the gene expression omnibus. Biophys Rev. 2019;11(1):103–110. doi:10.1007/s12551-018-0490-830594974
  • Dai F, Chen G, Wang Y, et al. Identification of candidate biomarkers correlated with the diagnosis and prognosis of cervical cancer via integrated bioinformatics analysis. Onco Targets Ther. 2019;12:4517–4532. doi:10.2147/OTT.S19961531354287
  • Liang B, Li Y, Wang T. A three miRNAs signature predicts survival in cervical cancer using bioinformatics analysis. Sci Rep. 2017;7(1):5624. doi:10.1038/s41598-017-06032-228717180
  • Lin H, Ma Y, Wei Y, Shang H. Genome-wide analysis of aberrant gene expression and methylation profiles reveals susceptibility genes and underlying mechanism of cervical cancer. Eur J Obstet Gynecol Reprod Biol. 2016;207:147–152. doi:10.1016/j.ejogrb.2016.10.01727863272
  • Choi K, Ratner N. iGEAK: an interactive gene expression analysis kit for seamless workflow using the R/shiny platform. BMC Genomics. 2019;20(1):177. doi:10.1186/s12864-019-5548-x30841853
  • Raudvere U, Kolberg L, Kuzmin I, et al. g: Profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update). Nucleic Acids Res. 2019;47(W1):W191–w198. doi:10.1093/nar/gkz36931066453
  • Szklarczyk D, Gable AL, Lyon D, et al. STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res. 2019;47(D1):D607–D613. doi:10.1093/nar/gky113130476243
  • Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z. GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017;45(W1):W98–w102. doi:10.1093/nar/gkx24728407145
  • Nagy Á, Lánczky A, Menyhárt O, Győrffy B. Validation of miRNA prognostic power in hepatocellular carcinoma using expression data of independent datasets. Sci Rep. 2018;8(1):9227. doi:10.1038/s41598-018-27521-y29907753
  • Fei L, Xu H. Role of MCM2-7 protein phosphorylation in human cancer cells. Cell Biosci. 2018;8:43. doi:10.1186/s13578-018-0242-230062004
  • Ishimi Y. Regulation of MCM2-7 function. Genes Genet Syst. 2018;93(4):125–133. doi:10.1266/ggs.18-0002630369561
  • Pollok S, Bauerschmidt C, Sänger J, Nasheuer HP, Grosse F. Human CDC45 is a proliferation-associated antigen. FEBS J. 2007;274(14):3669–3684. doi:10.1111/j.1742-4658.2007.05900.x17608804
  • Ke Y, Guo W, Huang S, et al. RYBP inhibits esophageal squamous cell carcinoma proliferation through downregulating CDC6 and CDC45 in G1-S phase transition process. Life Sci. 2020;250:117578. doi:10.1016/j.lfs.2020.11757832209426
  • Sun J, Shi R, Zhao S, et al. Cell division cycle 45 promotes papillary thyroid cancer progression via regulating cell cycle. Tumour Biology. 2017;39(5):1010428317705342. doi:10.1177/101042831770534228474999
  • Huang J, Li Y, Lu Z, et al. Analysis of functional hub genes identifies CDC45 as an oncogene in non-small cell lung cancer - a short report. Cellular Oncol. 2019;42(4):571–578. doi:10.1007/s13402-019-00438-y
  • Yang S, Ren X, Liang Y, et al. KNK437 restricts the growth and metastasis of colorectal cancer via targeting DNAJA1/CDC45 axis. Oncogene. 2020;39(2):249–261. doi:10.1038/s41388-019-0978-031477839
  • Hu Y, Wang L, Li Z, et al. Potential prognostic and diagnostic values of CDC6, CDC45, ORC6 and SNHG7 in colorectal cancer. Onco Targets Ther. 2019;12:11609–11621. doi:10.2147/OTT.S23194132021241
  • Sang L, Wang XM, Xu DY, Zhao WJ. Bioinformatics analysis of aberrantly methylated-differentially expressed genes and pathways in hepatocellular carcinoma. World J Gastroenterol. 2018;24(24):2605–2616. doi:10.3748/wjg.v24.i24.260529962817
  • Wu J, Lv Q, Huang H, Zhu M, Meng D. Screening and identification of key biomarkers in inflammatory breast cancer through integrated bioinformatic analyses. Genet Test Mol Biomarkers. 2020;24(8):484–491. doi:10.1089/gtmb.2020.004732598242
  • Liu J, Nie S, Gao M, et al. Identification of EPHX2 and RMI2 as two novel key genes in cervical squamous cell carcinoma by an integrated bioinformatic analysis. J Cell Physiol. 2019;234(11):21260–21273. doi:10.1002/jcp.2873131041817
  • Wen X, Liu S, Cui M. Effect of BRCA1 on the concurrent chemoradiotherapy resistance of cervical squamous cell carcinoma based on transcriptome sequencing analysis. Biomed Res Int. 2020;2020:3598417. doi:10.1155/2020/359841732685473
  • Zheng M, Zhou Y, Yang X, et al. High GINS2 transcript level predicts poor prognosis and correlates with high histological grade and endocrine therapy resistance through mammary cancer stem cells in breast cancer patients. Breast Cancer Res Treat. 2014;148(2):423–436. doi:10.1007/s10549-014-3172-725348432
  • Peng L, Song Z, Chen D, et al. GINS2 regulates matrix metallopeptidase 9 expression and cancer stem cell property in human triple negative Breast cancer. Biomed Pharmacother. 2016;84:1568–1574. doi:10.1016/j.biopha.2016.10.03227829549
  • Ye Y, Song YN, He SF, Zhuang JH, Wang GY, Xia W. GINS2 promotes cell proliferation and inhibits cell apoptosis in thyroid cancer by regulating CITED2 and LOXL2. Cancer Gene Ther. 2019;26(3–4):103–113. doi:10.1038/s41417-018-0045-y30177819
  • Shen YL, Li HZ, Hu YW, Zheng L, Wang Q. Loss of GINS2 inhibits cell proliferation and tumorigenesis in human gliomas. CNS Neurosci Ther. 2019;25(2):273–287. doi:10.1111/cns.1306430338650
  • Huang L, Chen S, Fan H, Ji D, Chen C, Sheng W. GINS2 promotes EMT in pancreatic cancer via specifically stimulating ERK/MAPK signaling. Cancer Gene Ther. 2020. doi:10.1038/s41417-020-0206-7
  • Chen T, Yang S, Xu J, Lu W, Xie X. Transcriptome sequencing profiles of cervical cancer tissues and SiHa cells. Funct Integr Genomics. 2020;20(2):211–221. doi:10.1007/s10142-019-00706-y31456134
  • Branca M, Ciotti M, Giorgi C, et al. Up-regulation of proliferating cell nuclear antigen (PCNA) is closely associated with high-risk human papillomavirus (HPV) and progression of cervical intraepithelial neoplasia (CIN), but does not predict disease outcome in cervical cancer. Eur J Obstet Gynecol Reprod Biol. 2007;130(2):223–231. doi:10.1016/j.ejogrb.2006.10.00717098349
  • Kim TH, Han JH, Shin E, Noh JH, Kim HS, Song YS. Clinical implication of p16, ki-67, and proliferating cell nuclear antigen expression in cervical neoplasia: improvement of diagnostic accuracy for high-grade squamous intraepithelial lesion and prediction of resection margin involvement on conization specimen. J Cancer Prevention. 2015;20(1):70–77.
  • Madhumati G, Kavita S, Anju M, Uma S, Raj M. Immunohistochemical expression of cell proliferating nuclear antigen (PCNA) and p53 protein in cervical cancer. J Obstet Gynaecol India. 2012;62(5):557–561. doi:10.1007/s13224-012-0180-624082558
  • Issac MSM, Yousef E, Tahir MR, Gaboury LA. MCM2, MCM4, and MCM6 in breast cancer: clinical utility in diagnosis and prognosis. Neoplasia. 2019;21(10):1015–1035. doi:10.1016/j.neo.2019.07.01131476594
  • Giaginis C, Georgiadou M, Dimakopoulou K, et al. Clinical significance of MCM-2 and MCM-5 expression in colon cancer: association with clinicopathological parameters and tumor proliferative capacity. Dig Dis Sci. 2009;54(2):282–291. doi:10.1007/s10620-008-0305-z18465232
  • Kuku S, Proctor I, Loddo M, et al. Do cell-cycle phase-specific markers predict disease grade, stage, and outcome in cervical carcinoma? Int J Gynecological Cancer. 2015;25(6):1066–1072. doi:10.1097/IGC.0000000000000356
  • Amaro Filho SM, Nuovo GJ, Cunha CB, et al. Correlation of MCM2 detection with stage and virology of cervical cancer. Int J Biol Markers. 2014;29(4):e363–371. doi:10.5301/jbm.500008124706378
  • Das M, Prasad SB, Yadav SS, et al. Over expression of minichromosome maintenance genes is clinically correlated to cervical carcinogenesis. PLoS One. 2013;8(7):e69607. doi:10.1371/journal.pone.006960723874974
  • Kaur G, Balasubramaniam SD, Lee YJ, Balakrishnan V, Oon CE. Minichromosome maintenance complex (MCM) genes profiling and MCM2 protein expression in cervical cancer development. Asian Pacific J Cancer Prevention. 2019;20(10):3043–3049. doi:10.31557/APJCP.2019.20.10.3043
  • Ishimi Y, Okayasu I, Kato C, et al. Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix. European J Biochem. 2003;270(6):1089–1101. doi:10.1046/j.1432-1033.2003.03440.x12631269
  • Deng M, Sun J, Xie S, et al. Inhibition of MCM2 enhances the sensitivity of ovarian cancer cell to carboplatin. Mol Med Rep. 2019;20(3):2258–2266.31322224
  • Wu W, Wang X, Shan C, Li Y, Li F. Minichromosome maintenance protein 2 correlates with the malignant status and regulates proliferation and cell cycle in lung squamous cell carcinoma. Onco Targets Ther. 2018;11:5025–5034. doi:10.2147/OTT.S16900230174440