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Original Research

Rapid simultaneous detection of blaoxa-23, Ade-B, int-1, and ISCR-1 in multidrug-resistant Acinetobacter baumannii using single-tube multiplex PCR and high resolution melting assay

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Pages 1573-1581 | Published online: 07 Jun 2019

References

  • Towner KJ. Acinetobacter: an old friend, but a new enemy. J Hosp Infect. 2009;73(4):355–363. doi:10.1016/j.jhin.2009.03.03219700220
  • Poulakou G, Renieris G, Sabrakos L, et al. Daptomycin as adjunctive treatment for experimental infection by Acinetobacter baumannii with resistance to col1istin. Int J Antimicrob AG. 2019;53(2):190–194. doi:10.1016/j.ijantimicag.2018.10.024
  • Schuertz KF, Tuon FF, Palmeiro JK, et al. Bacteremia and meningitis caused by OXA-23-producing Acinetobacter baumannii – molecular characterization and susceptibility testing for alternative antibiotics. Braz J Microbiol. 2018;49:199–204. doi:10.1016/j.bjm.2018.04.00229720352
  • Chuang Y, Cheng A, Sun H, et al. Microbiological and clinical characteristics of Acinetobacter baumannii bacteremia: implications of sequence type for prognosis. J Infect. 2019;78(2):106–112. doi:10.1016/j.jinf.2018.10.00130308215
  • Rocha IV, Xavier DE, Almeida KRHD, Oliveira SRD, Leal NC. Multidrug-resistant Acinetobacter baumannii clones persist on hospital inanimate surfaces. Braz J Infect Dis. 2018;22(5):438–441. doi:10.1016/j.bjid.2018.08.00430265856
  • Jain M, Sharma A, Sen MK, Rani V, Gaind R, Suri JC. Phenotypic and molecular characterization of Acinetobacter baumannii isolates causing lower respiratory infections among ICU patients. Microb Pathog. 2019;128:75–81. doi:10.1016/j.micpath.2018.12.02330562602
  • Lee M, Chen T, Lee Y, et al. Dissemination of multidrug-resistant Acinetobacter baumannii carrying BlaOxA-23 from hospitals in central Taiwan. J Microbiol Immunol Infect. 2013;46(6):419–424. doi:10.1016/j.jmii.2012.08.00623010539
  • Fournier PE, Vallenet D, Barbe V, et al. Comparative genomics of multidrug resistance in Acinetobacter baumannii. PLoS Genet. 2006;2(1):e7. doi:10.1371/journal.pgen.002000716415984
  • Jamal S, Al Atrouni A, Rafei R, Dabboussi F, Hamze M, Osman M. Molecular mechanisms of antimicrobial resistance in acinetobacter baumannii, with a special focus on its epidemiology in Lebanon. J Glob Antimicrob Re. 2018;15:154–163. doi:10.1016/j.jgar.2018.05.022
  • Charfi-Kessis K, Mansour W, Ben Haj Khalifa A, et al. Multidrug-resistant Acinetobacter baumannii strains carrying the blaOxA-23 and the blaGES-11 genes in a neonatology center in Tunisia. Microb Pathog. 2014;74:20–24. doi:10.1016/j.micpath.2014.07.00325057763
  • Da Silva KE, Maciel WG, Croda J, et al. A high mortality rate associated with multidrug-resistant Acinetobacter baumannii ST79 and ST25 carrying OXA-23 in a Brazilian intensive care unit. PLoS One. 2018;13(12):e209367. doi:10.1371/journal.pone.0209367
  • Petrović T, Uzunović S, Barišić I, et al. Arrival of carbapenem-hydrolyzing-oxacillinases in Acinetobacter baumannii in Bosnia and Herzegovina. Infect Genet Evol. 2018;58:192–198. doi:10.1016/j.meegid.2017.12.02129277554
  • Costa EM, Silva S, Vicente S, Veiga M, Tavaria F, Pintado MM. Chitosan as an effective inhibitor of multidrug resistant Acinetobacter baumannii. Carbohydr Polym. 2017;178:347–351. doi:10.1016/j.carbpol.2017.09.05529050604
  • Lee S, Oh MH, Yun SH, et al. Genomic characterization of extensively drug-resistant Acinetobacter baumannii strain, KAB03 belonging to ST451 from Korea. Infect Genet Evol. 2018;65:150–158. doi:10.1016/j.meegid.2018.07.03030053642
  • Almasaudi SB. Acinetobacter spp. as nosocomial pathogens: epidemiology and resistance features. Saudi J Biol Sci. 2018;25(3):586–596. doi:10.1016/j.sjbs.2016.02.00929686523
  • Poey ME, Laviña M. Horizontal transfer of class 1 integrons from uropathogenic Escherichia coli to E. coli K12. Microb Pathog. 2018;117:16–22. doi:10.1016/j.micpath.2018.02.00629428427
  • Liu C, Tang CY, Chang K, Kuo H, Liou M. A comparative study of class 1 integrons in acinetobacter baumannii. Gene. 2014;544(1):75–82. doi:10.1016/j.gene.2014.04.04724768721
  • Rui Y, Lu W, Li S, Cheng C, Sun J, Yang Q. Integrons and insertion sequence common region 1 (ISCR1) of carbapenem-non-susceptible gram-negative bacilli in fecal specimens from 5000 patients in southern China. Int J Antimicrob AG. 2018;52(5):571–576. doi:10.1016/j.ijantimicag.2018.06.015
  • Zheng F, Sun J, Cheng C, Rui Y. Molecular characteristics of carbapenem-resistant gram-negative bacteria in Southern China. Microb Drug Resist. 2015;21(2):178–185. doi:10.1089/mdr.2014.008525469995
  • Yong TB, Hashim R, Noor AM, Hamzah SH, Ahmad N. Identification of brucella spp. isolated from human brucellosis in Malaysia using high-resolution melt (HRM) analysis. Diagn Micr Infect Dis. 2015;81(4):227–233. doi:10.1016/j.diagmicrobio.2014.12.012
  • Pasanen T, Koskela S, Mero S, et al. Rapid molecular characterization of Acinetobacter baumannii clones with rep-PCR and evaluation of carbapenemase genes by new multiplex PCR in hospital district of helsinki and uusimaa. PLoS One. 2014;9(1):e85854. doi:10.1371/journal.pone.008585424465749
  • Lee Y, Yum JH, Kim C, et al. Role of OXA-23 and AdeABC efflux pump for acquiring carbapenem resistance in an Acinetobacter baumannii strain carrying the blaOXA-66 gene. Ann Clin Lab Sci. 2010;40(1):43–48.20124329
  • Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268–281. doi:10.1111/j.1469-0691.2011.03570.x21793988
  • Guan X, He L, Hu B, et al. Laboratory diagnosis, clinical management and infection control of the infections caused by extensively drug-resistant gram-negative bacilli: a Chinese consensus statement. Clin Microbiol Infect. 2016;22:S15–25. doi:10.1016/j.cmi.2015.11.00426627340
  • Roth AL, Hanson ND. Rapid detection and statistical differentiation of KPC gene variants in Gram-negative pathogens by use of high-resolution melting and ScreenClust analyses. J Clin Microbiol. 2013;51(1):61–65. doi:10.1128/JCM.02193-1223077125
  • Lin M, Lin Y, Tu C, Lan C. Distribution of different efflux pump genes in clinical isolates of multidrug-resistant Acinetobacter baumannii and their correlation with antimicrobial resistance. J Microbiol Immunol Infect. 2017;50(2):224–231. doi:10.1016/j.jmii.2015.04.00426055688
  • Pournaras S, Dafopoulou K, Del Franco M, et al. Predominance of international clone 2 OXA-23-producing-Acinetobacter baumannii clinical isolates in Greece, 2015: results of a nationwide study. Int J Antimicrob AG. 2017;49(6):749–753. doi:10.1016/j.ijantimicag.2017.01.028
  • Ramírez MS, Vilacoba E, Stietz MS, et al. Spreading of AbaR-type genomic islands in multidrug resistance acinetobacter baumannii strains belonging to different clonal complexes. Curr Microbiol. 2013;67(1):9–14. doi:10.1007/s00284-013-0326-523397241