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Original Research

Characterization of SCCmec, Spa Types and Multidrug Resistant of Methicillin-Resistant Staphylococcus aureus Isolates in Ahvaz, Iran

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Pages 1033-1044 | Published online: 09 Apr 2020

References

  • Struelens MJ, Hawkey PM, French GL, Witte W, Tacconelli E. Laboratory tools and strategies for methicillin-resistant Staphylococcus aureus screening, surveillance and typing: state of the art and unmet needs. Clin Microbiol Infect. 2009;15(2):112–119. doi:10.1111/j.1469-0691.2009.02698.x
  • Goetghebeur M, Landry PA, Han D, Vicente C. Methicillin-resistant Staphylococcus aureus: a public health issue with economic consequences. Can J Infect Dis Med Microbiol. 2007;18(1):27–34. doi:10.1155/2007/25394718923684
  • Dadashi M, Nasiri MJ, Fallah F, et al. Methicillin-resistant Staphylococcus aureus (MRSA) in Iran: a systematic review and meta-analysis. J Glob Antimicrob Resist. 2018;12:96–103. doi:10.1016/j.jgar.2017.09.00628941791
  • Ghanwate N, Thakare P, Bhise PR, Gawande S. Colorimetric method for rapid detection of Oxacillin resistance in Staphylococcus aureus and its comparison with PCR for mec A gene. Sci Rep. 2016;6(1):1–5. doi:10.1038/srep2301328442746
  • Boye K, Bartels MD, Andersen IS, Moeller JA, Westh H. A new multiplex PCR for easy screening of methicillin‐resistant Staphylococcus aureus SCCmec types I–V. Clin Microbiol Infect. 2007;13(7):725–727. doi:10.1111/j.1469-0691.2007.01720.x17403127
  • Chung M, De Lencastre H, Matthews P, et al; Multilaboratory Project Collaborators. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb Drug Resist. 2000;6(3):189–198. DOI:10.1089/mdr.2000.6.189.11144419
  • David MZ, Taylor A, Lynfield R, et al. Comparing pulsed-field gel electrophoresis with multilocus sequence typing, spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and PCR for Panton-Valentine leukocidin, arcA, and opp3 in methicillin-resistant Staphylococcus aureus isolates at a US medical center. J Clin Microbiol. 2013;51(3):814–819. doi:10.1128/JCM.02429-1223269731
  • Mayerhofer B, Stöger A, Pietzka AT, et al. Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis. PLoS One. 2015;10(3):e0116713. doi:10.1371/journal.pone.011671325768007
  • Fasihi Y, Fooladi S, Mohammadi MA, Emaneini M, Kalantar-Neyestanaki D. The spa typing of methicillin-resistant Staphylococcus aureus isolates by high resolution melting (HRM) analysis. J Med Microbiol. 2017;66(9):1335–1337. doi:10.1099/jmm.0.00057428875910
  • Ririe KM, Rasmussen RP, Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 1997;245(2):154–160. doi:10.1006/abio.1996.99169056205
  • Mazi W, Sangal V, Sandstrom G, Saeed A, Yu J. Evaluation of spa-typing of methicillin-resistant Staphylococcus aureus using high-resolution melting analysis. Int J Infect Dis. 2015;38:125–128. doi:10.1016/j.ijid.2015.05.00225975652
  • Tille P. Bailey & Scott’s Diagnostic Microbiology-E-Book. St. Louis, MO: Elsevier Health Sciences; 2015:307–327.
  • Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Sixteenth Informational Supplement; 2019
  • Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268–281. doi:10.1111/j.1469-0691.2011.03570.x21793988
  • Havaei SA, Azimian A, Fazeli H, et al. Genetic characterization of methicillin resistant and sensitive, vancomycin intermediate Staphylococcus aureus strains isolated from different Iranian Hospitals. ISRN Microbiol. 2012;20:2012. doi:10.5402/2012/215275
  • Moosavian M, Shahin M, Navidifar T, Torabipour M. Typing of staphylococcal cassette chromosome mec encoding methicillin resistance in Staphylococcus aureus isolates in. New Microbes New Infect. 2018;21:90–94. DOI:10.1016/j.nmni.2017.11.006 Ahvaz, Iran.29379604
  • Oliveira DC, Crisóstomo I, Santos-Sanches I, et al. Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus. J of clin microbiolo. 2001;39(2):574–580. doi:10.1128/JCM.39.2.574-580.2001
  • MRSA, CDC. Available from: https://www.cdc.gov/mrsa/index.html. Accessed 84, 2019.
  • Abbasi-Montazeri E, Khosravi AD, Feizabadi MM, et al. The prevalence of methicillin resistant Staphylococcus aureus (MRSA) isolates with high-level mupirocin resistance from patients and personnel in a burn center. Burns. 2013;39(4):650–654. doi:10.1016/j.burns.2013.02.00523499497
  • Khosravi AD, Jenabi A, Montazeri EA. Distribution of genes encoding resistance to aminoglycoside modifying enzymes in methicillin-resistant Staphylococcus aureus (MRSA) strains. Kaohsiung J Med Sci. 2017;33(12):587–593. doi:10.1016/j.kjms.2017.08.00129132547
  • Khoshnood S, Shahi F, Jomehzadeh N, et al. Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients. Acta Microbiol Immunol Hung. 2019;17:1–2. doi:10.1556/030.66.2019.015
  • Nikfar R, Shamsizadeh A, Kajbaf TZ, Panah MK, Khaghani S, Moghddam M. Frequency of methicillin-resistant Staphylococcus aureus nasal carriage in healthy children. Iran J Microbiol. 2015;7:67.26622966
  • Turlej AG, Hryniewicz WA, Empel J. Staphylococcal cassette chromosome mec (Sccmec) classification and typing methods: an overview. Pol J Microbiol. 2011;60(2):95–103. doi:10.33073/pjm-21905625
  • Denis O, Jans B, Deplano A, et al. Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) among residents of nursing homes in Belgium. J Antimicrob Agents. 2009;64(6):1299–1306. doi:10.1093/jac/dkp345
  • Vindel A, Cuevas O, Cercenado E, et al. Methicillin-resistant Staphylococcus aureus in spain: molecular epidemiology and utility of different typing methods. J Clin Microbiol. 2009;47(6):1620–1627. doi:10.1128/JCM.01579-0819339473
  • Saba CK, Amenyona JK, Kpordze SW. Prevalence and pattern of antibiotic resistance of Staphylococcus aureus isolated from door handles and other points of contact in public hospitals in Ghana. Antimicrob Resist Infect Control. 2017;6(1):44. doi:10.1186/s13756-017-0203-228491294
  • Husain A, Rawat V, Umesh MK, Verma PK. Vancomycin, linezolid and daptomycin susceptibility pattern among clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Sub- Himalayan Center. J Lab Physicians. 2018;10(02):145. doi:10.4103/JLP.JLP_92_1729692578
  • Abdolmaleki Z, Mashak Z, Dehkordi FS. Phenotypic and genotypic characterization of antibiotic resistance in the methicillin-resistant Staphylococcus aureus strains isolated from hospital cockroaches. Antimicrob Resist Infect Control. 2019;8(1):54. doi:10.1186/s13756-019-0505-730911380
  • Huang YC, Hwang KP, Chen PY, Chen CJ, Lin TY. Prevalence of methicillin-resistant Staphylococcus aureus nasal colonization among Taiwanese children in 2005 and 2006. J Clin Microbiol. 2007;45(12):3992–3995. doi:10.1128/JCM.01202-0717942647
  • Khan SU, Mahmud MN, Chowdhury MA, Hakim MA. Prevalence of multidrug resistant Staphylococcus aureus isolates in clinical specimens collected from local patients of Chittagong, Bangladesh. Chittagong Univ J Biol Sci. 2011;61:75–85.
  • Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathog Glob Health. 2015;109(7):309–318. doi:10.1179/2047773215Y.000000003026343252
  • Ullah A, Qasim M, Rahman H, et al. High frequency of methicillin-resistant Staphylococcus aureus in Peshawar Region of Pakistan. Springerplus. 2016;5(1):600. doi:10.1186/s40064-016-2277-327247896
  • Kuhn G, Francioli P, Blanc DS. Double-locus sequence typing using clfB and spa, a fast and simple method for epidemiological typing of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2007;45(1):54–62. doi:10.1128/JCM.01457-0617093014
  • Wang FD, Wu PF, Chen SJ. Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources. J Microbiol Immunol Infect. 2019;52(3):426–432. doi:10.1016/j.jmii.2019.01.00130686615
  • Tong SY, Lilliebridge RA, Holt DC, McDonald MI, Currie BJ, Giffard PM. High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from northern Australia. Clin Microbiol Infect. 2009;15(12):1126–1131. doi:10.1111/j.1469-0691.2009.02732.x19392885
  • Bozdoğan B, Yıldız O, Oryaşın E, et al. t030 is the most common spa type among methicillin-resistant Staphylococcus aureus strains isolated from Turkish hospitals. Mikrobiyol Bul. 2013;47(4):571–581. doi:10.5578/mb.577024237426