814
Views
19
CrossRef citations to date
0
Altmetric
Original Research

Analysis of Antibiotic Resistance and Virulence Traits (Genetic and Phenotypic) in Klebsiella pneumoniae Clinical Isolates from Pakistan: Identification of Significant Levels of Carbapenem and Colistin Resistance

, , ORCID Icon, & ORCID Icon
Pages 227-236 | Published online: 25 Jan 2021

References

  • Xu L, Sun X, Ma X. Systematic review and meta-analysis of mortality of patients infected with carbapenem-resistant Klebsiella pneumoniae. Ann Clin Microbiol Antimicrob. 2017;16(1):18. doi:10.1186/s12941-017-0191-328356109
  • Center for Disease Dynamics E, Policy. State of the World’s Antibiotics, 2015. Washington, DC: CDDEP; 2015.
  • Paczosa MK, Mecsas J. Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev. 2016;80:629–661.27307579
  • Brolund A, Lagerqvist N, Byfors S, et al. Worsening epidemiological situation of carbapenemase-producing Enterobacteriaceae in Europe, assessment by national experts from 37 countries, July 2018. Eurosurveillance. 2019;24(9):1900123. doi:10.2807/1560-7917.ES.2019.24.9.1900123
  • Gupta N, Limbago BM, Patel JB, Kallen AJ. Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin Infect Dis. 2011;53:60–67. doi:10.1093/cid/cir20221653305
  • Shon AS, Bajwa RP, Russo TA. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence. 2013;4:107–118. doi:10.4161/viru.2271823302790
  • Khan E, Ejaz M, Zafar A, et al. Increased isolation of ESBL producing Klebsiella pneumoniae with emergence of carbapenem resistant isolates in Pakistan: report from a tertiary care hospital. J Pak Med Assoc. 2010;60:186.20225774
  • Kamarasamy K, Toleman M, Walsh T. Emergence of a new antibiotic resistance in India, Pakistan, and the UK: a prospective survey. Lancet Infect Dis. 2010;10:597–602. doi:10.1016/S1473-3099(10)70143-220705517
  • Qamar S, Shaheen N, Shakoor S, Farooqi J, Jabeen K, Hasan R. Frequency of colistin and fosfomycin resistance in carbapenem-resistant Enterobacteriaceae from a tertiary care hospital in Karachi. Infect Drug Resist. 2017;10:231. doi:10.2147/IDR.S13677728814888
  • Aslam B, Chaudhry TH, Arshad MI, et al. The first bla KPC harboring Klebsiella pneumoniae ST258 strain isolated in Pakistan. Microb Drug Resist. 2020;26(7):783–786. doi:10.1089/mdr.2019.042032109182
  • Gharrah MM, Mostafa El-Mahdy A, Barwa RF. Association between virulence factors and extended spectrum beta-lactamase producing Klebsiella pneumoniae compared to nonproducing isolates. Interdiscip Perspect Infect Dis. 2017;2017:1–14. doi:10.1155/2017/7279830
  • Wayne P. Clinical and Laboratory Standards Institute: performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI Doc M100-S20. 2010.
  • McKiernan H, Danielson P. Molecular diagnostic applications in forensic science In: Molecular Diagnostics. Third ed. Elsevier; 2017:371–394.
  • El Fertas-Aissani R, Messai Y, Alouache S, Bakour R. Virulence profiles and antibiotic susceptibility patterns of Klebsiella pneumoniae strains isolated from different clinical specimens. Pathol Biol. 2013;61(5):209–216. doi:10.1016/j.patbio.2012.10.00423218835
  • England PH. English Surveillance Programme for Antimicrobial Utilisation and Resistance (ESPAUR) Report 2018–2019. London, UK: PHE; 2019.
  • Hawser SP, Bouchillon SK, Lascols C, et al. Susceptibility of Klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates. Antimicrob Agents Chemother. 2011;55(8):3917–3921. doi:10.1128/AAC.00070-1121670192
  • Behera B, Mathur P, Das A, Kapil A. Ertapenem susceptibility of extended spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary care centre in India. Singapore Med J. 2009;50(6):628.19551319
  • Russo TA, Marr CM. Hypervirulent Klebsiella pneumoniae. Clin Microbiol Rev. 2019;32(3):32. doi:10.1128/CMR.00001-19
  • Wang B, Pan F, Wang C, et al. Molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae in a paediatric hospital in China. Int J Infect Dis. 2020.
  • Kim D, Park BY, Choi MH, et al. Antimicrobial resistance and virulence factors of Klebsiella pneumoniae affecting 30 day mortality in patients with bloodstream infection. J Antimicrob Chemother. 2019;74(1):190–199. doi:10.1093/jac/dky39730295771
  • Mansury D, Motamedifar M, Sarvari J, Shirazi B, Khaledi A. Antibiotic susceptibility pattern and identification of extended spectrum β-lactamases (ESBLs) in clinical isolates of Klebsiella pneumoniae from Shiraz, Iran. Iran J Microbiol. 2016;8(1):55.27092225
  • Livermore D. Advice on Carbapenemase Producers: Recognition, Infection Control and Treatment. London: Health Protection Agency; 2011.
  • Gautam V, Thakur A, Sharma M, et al. Molecular characterization of extended-spectrum β-lactamases among clinical isolates of Escherichia coli & Klebsiella pneumoniae: a multi-centric study from tertiary care hospitals in India. Indian J Med Res. 2019;149(2):208. doi:10.4103/ijmr.IJMR_172_1831219085
  • Effah CY, Sun T, Liu S, Wu Y. Klebsiella pneumoniae: an increasing threat to public health. Ann Clin Microbiol Antimicrob. 2020;19(1):1–9. doi:10.1186/s12941-019-0343-831918737
  • Tumbarello M, Trecarichi EM, De Rosa FG, et al. Infections caused by KPC-producing Klebsiella pneumoniae: differences in therapy and mortality in a multicentre study. J Antimicrob Chemother. 2015;70(7):2133–2143. doi:10.1093/jac/dkv08625900159
  • Jayol A, Nordmann P, Brink A, Poirel L. Heteroresistance to colistin in Klebsiella pneumoniae associated with alterations in the PhoPQ regulatory system. Antimicrob Agents Chemother. 2015;AAC:05055.
  • Pragasam AK, Shankar C, Veeraraghavan B, et al. Molecular mechanisms of colistin resistance in Klebsiella pneumoniae causing bacteremia from India—a first report. Front Microbiol. 2017;7:2135. doi:10.3389/fmicb.2016.0213528119670
  • Mitra S, Mukherjee S, Naha S, Chattopadhyay P, Dutta S, Basu S. Evaluation of co-transfer of plasmid-mediated fluoroquinolone resistance genes and blaNDM gene in Enterobacteriaceae causing neonatal septicaemia. Antimicrob Resist Infect Control. 2019;8(1):46. doi:10.1186/s13756-019-0477-730858970
  • Liu -Y-Y, Wang Y, Walsh TR, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161–168. doi:10.1016/S1473-3099(15)00424-726603172
  • Testing ECoAS. Recommendations for MIC Determination of Colistin (Polymyxin E) as Recommended by the Joint CLSI-EUCAST Polymyxin Breakpoints Working Group. Växjö, Sweden: European Committee on Antimicrobial Susceptibility Testing; 2016 Available from:: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/General_documents/Recommendations_for_MIC_determination_of_colistin_March_2016.pdf. Accessed 112, 2021.
  • Xu Y, Lin J, Cui T, Srinivas S, Feng Y. Mechanistic insights into transferable polymyxin resistance among gut bacteria. J Biol Chem. 2018;jbc. RA117:000924.
  • Dalmolin TV, Bianchini BV, Rossi GG, et al. Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae. Braz J Microbiol. 2017;48(3):493–498. doi:10.1016/j.bjm.2017.01.00328291698
  • Khan FA, Hellmark B, Ehricht R, Söderquist B, Jass J. Related carbapenemase-producing Klebsiella isolates detected in both a hospital and associated aquatic environment in Sweden. Eur J Clin Microbiol Infect Dis. 2018;37(12):2241–2251. doi:10.1007/s10096-018-3365-930171482
  • Sultan BA, Irfan S. Detection of metallo-beta lactamases (IMP, VIM, NDM) and KPC carbapenemases in carbapenem resistant Enterobacteriaceae: report from Clinical Laboratory Aga Khan University Karachi, Pakistan. Infect Dis J Pak. 2014;584.
  • Lomonaco S, Crawford MA, Lascols C, et al. Resistome of carbapenem- and colistin-resistant Klebsiella pneumoniae clinical isolates. PLoS One. 2018;13(6):e0198526. doi:10.1371/journal.pone.019852629883490
  • Siu LK, Yeh K-M, Lin J-C, Fung C-P, Chang F-Y. Klebsiella pneumoniae liver abscess: a new invasive syndrome. Lancet Infect Dis. 2012;12(11):881–887. doi:10.1016/S1473-3099(12)70205-023099082
  • Sayeed MA, Latif N, Mahmood SF. Hypermucoviscous Klebsiella syndrome it’s in the community! J Pak Med Assoc. 2017;67:1930–1932.29256546
  • Jung S, Chae H, Park Y, et al. Microbiological and clinical characteristics of bacteraemia caused by the hypermucoviscosity phenotype of Klebsiella pneumoniae in Korea. Epidemiol Infect. 2013;141(2):334–340. doi:10.1017/S095026881200093322578630
  • Qu -T-T, Zhou J-C, Jiang Y, et al. Clinical and microbiological characteristics of Klebsiella pneumoniae liver abscess in East China. BMC Infect Dis. 2015;15(1):161. doi:10.1186/s12879-015-0899-725886859
  • Hennequin C, Robin F. Correlation between antimicrobial resistance and virulence in Klebsiella pneumoniae. Eur J Clin Microbiol Infect Dis. 2016;35(3):333–341. doi:10.1007/s10096-015-2559-726718943
  • Wyres KL, Nguyen TNT, Lam MMC, et al. Genomic surveillance for hypervirulence and multi-drug resistance in invasive Klebsiella pneumoniae from South and Southeast Asia. Genome Med. 2020;12(1):1–16. doi:10.1186/s13073-019-0706-y