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Original Research

Dependence of Xpert MTB/RIF Accuracy for Detecting Rifampin Resistance in Bronchoalveolar Lavage Fluid on Bacterial Load: A Retrospective Study in Beijing, China

, , , , , , , , & ORCID Icon show all
Pages 2429-2435 | Published online: 28 Jun 2021

References

  • World Health Organization. Global Tuberculosis Report 2020. Geneva: World Health Organization; 2020. Licence: CC BY-NC-SA 3.0 IGO.
  • Chakaya JM, Harries AD, Marks GB. Ending tuberculosis by 2030-pipe dream or reality? Int J Infect Dis. 2020;92S:S51–S4. doi:10.1016/j.ijid.2020.02.02132114202
  • Dheda K, Gumbo T, Maartens G, et al. The epidemiology, pathogenesis, transmission, diagnosis, and management of multidrug-resistant, extensively drug-resistant, and incurable tuberculosis. Lancet Respir Med. 2017.
  • van Cutsem G, Isaakidis P, Farley J, Nardell E, Volchenkov G, Cox H. Infection control for drug-resistant tuberculosis: early diagnosis and treatment is the key. Clin Infect Dis. 2016;62(Suppl 3):S238–43. doi:10.1093/cid/ciw01227118853
  • Siddiqi K, Lambert ML, Walley J. Clinical diagnosis of smear-negative pulmonary tuberculosis in low-income countries: the current evidence. Lancet Infect Dis. 2003;3(5):288–296. doi:10.1016/S1473-3099(03)00609-112726978
  • Schon T, Miotto P, Koser CU, Viveiros M, Bottger E, Cambau E. Mycobacterium tuberculosis drug-resistance testing: challenges, recent developments and perspectives. Clin Microbiol Infect. 2017;23(3):154–160. doi:10.1016/j.cmi.2016.10.02227810467
  • Pang Y, Xia H, Zhang Z, et al. Multicenter evaluation of genechip for detection of multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol. 2013;51(6):1707–1713. doi:10.1128/JCM.03436-1223515537
  • Parsons LM, Somoskovi A, Gutierrez C, et al. Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities. Clin Microbiol Rev. 2011;24(2):314–350. doi:10.1128/CMR.00059-1021482728
  • MacLean E, Kohli M, Weber SF, et al. Advances in molecular diagnosis of tuberculosis. J Clin Microbiol. 2020;58(10). doi:10.1128/JCM.01582-19
  • World Health Oragnization. Automated Real-Time Nucleic Acid Amplification Technology for Rapid and Simultaneous Detection of Tuberculosis and Rifampicin Resistance: Xpert MTB/RIF Assay for the Diagnosis of Pulmonary and Extrapulmonary TB in Adults and Children. Geneva, Switherland: World Health Organization; 2013. WHO/HTM/TB/2013.16.
  • Van Rie A, Mellet K, John MA, et al. False-positive rifampicin resistance on Xpert(R) MTB/RIF: case report and clinical implications. Int J Tuberc Lung Dis. 2012;16(2):206–208. doi:10.5588/ijtld.11.039522236921
  • Ocheretina O, Byrt E, Mabou MM, et al. False-positive rifampin resistant results with Xpert MTB/RIF version 4 assay in clinical samples with a low bacterial load. Diagn Microbiol Infect Dis. 2016;85(1):53–55. doi:10.1016/j.diagmicrobio.2016.01.00926915638
  • Xu P, Tang P, Song H, et al. The incremental value of bronchoalveolar lavage for the diagnosis of pulmonary tuberculosis in a high-burden urban setting. J Infect. 2019;79(1):24–29. doi:10.1016/j.jinf.2019.05.00931100361
  • Theron G, Peter J, Meldau R, et al. Accuracy and impact of Xpert MTB/RIF for the diagnosis of smear-negative or sputum-scarce tuberculosis using bronchoalveolar lavage fluid. Thorax. 2013;68(11):1043–1051. doi:10.1136/thoraxjnl-2013-20348523811536
  • Huo F, Ma Y, Liu R, et al. Interpretation of discordant rifampicin susceptibility test results obtained using genexpert vs phenotypic drug susceptibility testing. Open Forum Infect Dis. 2020;7(8):ofaa279. doi:10.1093/ofid/ofaa27932766385
  • Pang Y, Liu G, Wang Y, Zheng S, Zhao YL. Combining COLD-PCR and high-resolution melt analysis for rapid detection of low-level, rifampin-resistant mutations in Mycobacterium tuberculosis. J Microbiol Methods. 2013;93(1):32–36. doi:10.1016/j.mimet.2013.01.00823396215
  • Lawn SD, Mwaba P, Bates M, et al. Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test. Lancet Infect Dis. 2013;13(4):349–361. doi:10.1016/S1473-3099(13)70008-223531388
  • Mathys V, van de Vyvere M, de Droogh E, Soetaert K, Groenen G. False-positive rifampicin resistance on Xpert(R) MTB/RIF caused by a silent mutation in the rpoB gene. Int J Tuberc Lung Dis. 2014;18(10):1255–1257. doi:10.5588/ijtld.14.029725216843
  • Ngabonziza JCS, Decroo T, Migambi P, et al. Prevalence and drivers of false-positive rifampicin-resistant Xpert MTB/RIF results: a prospective observational study in Rwanda. Lancet Microbe. 2020;1(2):e74–e83. doi:10.1016/S2666-5247(20)30007-0
  • Jing W, Pang Y, Zong Z, et al. Rifabutin resistance associated with double mutations in rpoB gene in Mycobacterium tuberculosis isolates. Front Microbiol. 2017;8:1768. doi:10.3389/fmicb.2017.0176828959248
  • Zhang Z, Wang Y, Pang Y, Liu C. Comparison of different drug susceptibility test methods to detect rifampin heteroresistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2014;58(9):5632–5635. doi:10.1128/AAC.02778-1425022589
  • Qi XY, Qiu XS, Jiang JY, Chen YX, Tang LM, Shi HF. Microwaves increase the effectiveness of systemic antibiotic treatment in acute bone infection: experimental study in a rat model. J Orthop Surg Res. 2019;14(1):286. doi:10.1186/s13018-019-1342-331488167