256
Views
3
CrossRef citations to date
0
Altmetric
ORIGINAL RESEARCH

Evaluation of the Performance of a Multiplex Real-Time PCR Assay for the Identification of Aspergillus, Cryptococcus neoformans, and Pneumocystis jirovecii Simultaneously from Sputum in Multicenter

, , , , , , , ORCID Icon & show all
Pages 6009-6017 | Received 18 Jun 2022, Accepted 28 Sep 2022, Published online: 18 Oct 2022

References

  • Donnelly JP, Chen SC, Kauffman CA, et al. Revision and update of the consensus definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium. Clin Infect Dis. 2020;71(6):1367–1376. doi:10.1093/cid/ciz1008
  • Schmiedel Y, Zimmerli S. Common invasive fungal diseases: an overview of invasive candidiasis, aspergillosis, cryptococcosis, and Pneumocystis pneumonia. Swiss Med Wkly. 2016;146:w14281. doi:10.4414/smw.2016.14281
  • Gago S, Esteban C, Valero C, et al. A multiplex real-time PCR assay for identification of Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii in samples from AIDS patients with opportunistic pneumonia. J Clin Microbiol. 2014;52:1168–1176. doi:10.1128/jcm.02895-13
  • Bateman M, Oladele R, Kolls JK. Diagnosing Pneumocystis jirovecii pneumonia: a review of current methods and novel approaches. Med Mycol. 2020;58:1015–1028. doi:10.1093/mmy/myaa024
  • Kidd SE, Chen SC, Meyer W, et al. A new age in molecular diagnostics for invasive fungal disease: Are we ready? Front Microbiol. 2019;10:2903. doi:10.3389/fmicb.2019.02903
  • Von Lilienfeld-Toal M, Wagener J, Einsele H, et al. Invasive fungal infection. Dtsch Arztebl Int. 2019;116:271–278. doi:10.3238/arztebl.2019.0271
  • Day JN, Qihui S, Thanh LT, et al. Comparative genomics of Cryptococcus neoformans var. grubii associated with meningitis in HIV infected and uninfected patients in Vietnam. PLoS Negl Trop Dis. 2017;11:e0005628. doi:10.1371/journal.pntd.0005628
  • Player R, Verratti K, Staab A, et al. Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens. BMC Genomics. 2020;21:166. doi:10.1186/s12864-020-6557-5
  • Kilic A, Elliott S, Hester L, et al. Evaluation of the performance of DiaSorin molecular Pneumocystis jirovecii-CMV multiplex real-time PCR assay from bronchoalveolar lavage samples. J Mycol Med. 2020;30:100936. doi:10.1016/j.mycmed.2020.100936
  • Gu Z, Buelow DR, Petraitiene R, et al. Quantitative multiplexed detection of common pulmonary fungal pathogens by labeled primer polymerase chain reaction. Arch Pathol Lab Med. 2014;138(11):1474–1480. doi:10.5858/arpa.2013-0592-OA
  • Takahashi Y, Osawa R, Kubota Y, et al. Early diagnosis of Cryptococcus neoformans var. grubii meningitis using multiplex PCR assay in an immunocompetent patient. J Infect Chemother. 2021;27(12):1765–1768. doi:10.1016/j.jiac.2021.08.006
  • Clinical and Laboratory Standards Institute. Interpretive Criteria for Identification of Bacteria and Fungi by Targeted DNA Sequencing. 2nd ed. Wayne PA: Clinical and Laboratory Standards Institute; 2018.
  • Ala-Houhala M, Koukila-Kähkölä P, Antikainen J, et al. Clinical use of fungal PCR from deep tissue samples in the diagnosis of invasive fungal diseases: a retrospective observational study. Clin Microbiol Infect. 2018;24(3):301–305. doi:10.1016/j.cmi.2017.08.017
  • Pathakumari B, Liang G, Liu W. Immune defence to invasive fungal infections: a comprehensive review. Biomed Pharmacother. 2020;130:110550. doi:10.1016/j.biopha.2020.110550
  • Ibanez-Martinez E, Ruiz-Gaitan A, Peman-Garcia J. Update on the diagnosis of invasive fungal infection. Rev Esp Quimioter. 2017;30(Suppl 1):16–21.
  • Sokulska M, Kicia M, Wesolowska M, et al. Pneumocystis jirovecii--from a commensal to pathogen: clinical and diagnostic review. Parasitol Res. 2015;114:3577–3585. doi:10.1007/s00436-015-4678-6
  • Alanio A, Desoubeaux G, Sarfati C, et al. Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients. Clin Microbiol Infect. 2011;17:1531–1537. doi:10.1111/j.1469-0691.2010.03400.x