Identification of Three Potential circRNA Biomarkers of Polycystic Ovary Syndrome by Bioinformatics Analysis and Validation

References

• Huang X, Wu B, Chen M, et al. Depletion of exosomal circLDLR in follicle fluid derepresses miR-1294 function and inhibits estradiol production via CYP19A1 in polycystic ovary syndrome. Aging. 2020;12(15):15414–15435. doi:10.18632/aging.103602
   PubMedGoogle Scholar
• Christakou C, Diamanti-Kandarakis E. Polycystic ovary syndrome–phenotypes and diagnosis. Scand J Clin Lab Invest Suppl. 2014;244:18–22; discussion 21. doi:10.3109/00365513.2014.936675
   PubMedGoogle Scholar
• Kandaraki E, Christakou C, Diamanti-Kandarakis E. Metabolic syndrome and polycystic ovary syndrome … and vice versa. Arq Bras Endocrinol Metabol. 2009;53(2):227–237. doi:10.1590/S0004-27302009000200014
   PubMedGoogle Scholar
• Carmina E, Bucchieri S, Mansueto P, Rini G, Ferin M, Lobo RA. Circulating levels of adipose products and differences in fat distribution in the ovulatory and anovulatory phenotypes of polycystic ovary syndrome. Fertil Steril. 2009;91(4 Suppl):1332–1335. doi:10.1016/j.fertnstert.2008.03.007
   PubMed Web of Science ®Google Scholar
• Matzuk MM, Burns KH, Viveiros MM, Eppig JJ. Intercellular communication in the mammalian ovary: oocytes carry the conversation. Science. 2002;296(5576):2178–2180. doi:10.1126/science.1071965
   PubMed Web of Science ®Google Scholar
• Rodgers RJ, Irving-Rodgers HF. Formation of the ovarian follicular antrum and follicular fluid. Biol Reprod. 2010;82(6):1021–1029. doi:10.1095/biolreprod.109.082941
   PubMed Web of Science ®Google Scholar
• Ambekar AS, Nirujogi RS, Srikanth SM, et al. Proteomic analysis of human follicular fluid: a new perspective towards understanding folliculogenesis. J Proteomics. 2013;87:68–77. doi:10.1016/j.jprot.2013.05.017
   PubMed Web of Science ®Google Scholar
• Tu M, Wu Y, Mu L, Zhang D. Long non-coding RNAs: novel players in the pathogenesis of polycystic ovary syndrome. Ann Transl Med. 2021;9(2):173. doi:10.21037/atm-20-5044
   PubMed Web of Science ®Google Scholar
• Lefevre PLC, Nardelli TC, Son WY, et al. Polybrominated diphenyl ethers in human follicular fluid dysregulate mural and cumulus granulosa cell gene expression. Endocrinology. 2021;162(3). doi:10.1210/endocr/bqab003
   PubMed Web of Science ®Google Scholar
• Zhao H, Wang L, Wang Y. Circulating microRNAs as candidate biomarkers for the ovarian response during in vitro fertilization. Medicine. 2021;100(6):e24612. doi:10.1097/MD.0000000000024612
   PubMed Web of Science ®Google Scholar
• Wang LP, Peng XY, Lv XQ, et al. High throughput circRNAs sequencing profile of follicle fluid exosomes of polycystic ovary syndrome patients. J Cell Physiol. 2019;234(9):15537–15547.
   PubMed Web of Science ®Google Scholar
• Cai H, Chang T, Li Y, et al. Circular DDX10 is associated with ovarian function and assisted reproductive technology outcomes through modulating the proliferation and steroidogenesis of granulosa cells. Aging. 2021;13(7):9592–9612. doi:10.18632/aging.202699
   PubMedGoogle Scholar
• Zhou WY, Cai ZR, Liu J, Wang DS, Ju HQ, Xu RH. Circular RNA: metabolism, functions and interactions with proteins. Mol Cancer. 2020;19(1):172. doi:10.1186/s12943-020-01286-3
   PubMed Web of Science ®Google Scholar
• Verduci L, Tarcitano E, Strano S, Yarden Y, Blandino G. CircRNAs: role in human diseases and potential use as biomarkers. Cell Death Dis. 2021;12(5):468. doi:10.1038/s41419-021-03743-3
   PubMed Web of Science ®Google Scholar
• Huang S, Yang B, Chen BJ, et al. The emerging role of circular RNAs in transcriptome regulation. Genomics. 2017;109(5–6):401–407. doi:10.1016/j.ygeno.2017.06.005
   PubMed Web of Science ®Google Scholar
• Sheng JQ, Liu L, Wang MR, Li PY. Circular RNAs in digestive system cancer: potential biomarkers and therapeutic targets. Am J Cancer Res. 2018;8(7):1142–1156.
   PubMed Web of Science ®Google Scholar
• Wang C, Jiang Y, Lei Q, et al. Potential diagnostic and prognostic biomarkers of circular RNAs for lung cancer in China. Biomed Res Int. 2019;2019:8023541.
   PubMed Web of Science ®Google Scholar
• Taborda MI, Ramirez S, Bernal G. Circular RNAs in colorectal cancer: possible roles in regulation of cancer cells. World J Gastrointest Oncol. 2017;9(2):62–69. doi:10.4251/wjgo.v9.i2.62
   PubMed Web of Science ®Google Scholar
• Wang S, Zhang K, Tan S, et al. Circular RNAs in body fluids as cancer biomarkers: the new frontier of liquid biopsies. Mol Cancer. 2021;20(1):13. doi:10.1186/s12943-020-01298-z
   PubMed Web of Science ®Google Scholar
• Xie L, Mao M, Xiong K, Jiang B. Circular RNAs: a novel player in development and disease of the central nervous system. Front Cell Neurosci. 2017;11:354. doi:10.3389/fncel.2017.00354
   PubMed Web of Science ®Google Scholar
• Li L, Ni Z, Si X, et al. Emerging clues of regulatory roles of circular RNAs through modulating oxidative stress: focus on neurological and vascular diseases. Oxid Med Cell Longev. 2021;2021:6659908.
   PubMed Web of Science ®Google Scholar
• Ward Z, Pearson J, Schmeier S, Cameron V, Pilbrow A. Insights into circular RNAs: their biogenesis, detection, and emerging role in cardiovascular disease. RNA Biol. 2021;1–18. doi:10.1080/15476286.2021.1891393
   Web of Science ®Google Scholar
• Kishore R, Garikipati VNS, Gonzalez C. Role of circular RNAs in cardiovascular disease. J Cardiovasc Pharmacol. 2020;76(2):128–137. doi:10.1097/FJC.0000000000000841
   PubMed Web of Science ®Google Scholar
• Zhang L, Zhang Y, Wang Y, Zhao Y, Ding H, Li P. Circular RNAs: functions and clinical significance in cardiovascular disease. Front Cell Dev Biol. 2020;8:584051. doi:10.3389/fcell.2020.584051
   PubMed Web of Science ®Google Scholar
• Liu H, Zou Y, Chen C, Tang Y, Guo J. Current understanding of circular RNAs in systemic lupus erythematosus. Front Immunol. 2021;12:628872. doi:10.3389/fimmu.2021.628872
   PubMed Web of Science ®Google Scholar
• Wu G, Xia J, Yang Z, et al. CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in polycystic ovary syndrome. J Cell Mol Med. 2020. doi:10.1111/jcmm.16231
   Web of Science ®Google Scholar
• Deng L, Chen Q, Xie J, Wei W, Hui H. circPUM1 promotes polycystic ovary syndrome progression by sponging to miR-760. Gene. 2020;754:144903. doi:10.1016/j.gene.2020.144903
   PubMed Web of Science ®Google Scholar
• Ebbesen KK, Hansen TB, Kjems J. Insights into circular RNA biology. RNA Biol. 2017;14(8):1035–1045. doi:10.1080/15476286.2016.1271524
   PubMed Web of Science ®Google Scholar
• Che Q, Liu M, Xu J, et al. Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome. Fertil Steril. 2019;111(6):1243–1251 e1241. doi:10.1016/j.fertnstert.2019.02.023
   PubMed Web of Science ®Google Scholar
• Ma Z, Zhao H, Zhang Y, Liu X, Hao C. Novel circular RNA expression in the cumulus cells of patients with polycystic ovary syndrome. Arch Gynecol Obstet. 2019;299(6):1715–1725. doi:10.1007/s00404-019-05122-y
   PubMed Web of Science ®Google Scholar
• Mao Z, Li T, Zhao H, Qin Y, Wang X, Kang Y. Identification of epigenetic interactions between microRNA and DNA methylation associated with polycystic ovarian syndrome. J Hum Genet. 2021;66(2):123–137. doi:10.1038/s10038-020-0819-6
   PubMed Web of Science ®Google Scholar
• Huang da W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4(1):44–57. doi:10.1038/nprot.2008.211
   PubMed Web of Science ®Google Scholar
• Wong N, Wang X. miRDB: an online resource for microRNA target prediction and functional annotations. Nucleic Acids Res. 2015;43(Databaseissue):D146–152. doi:10.1093/nar/gku1104
   PubMed Web of Science ®Google Scholar
• Fromm B, Billipp T, Peck LE, et al. A uniform system for the annotation of vertebrate microRNA genes and the evolution of the human microRNAome. Annu Rev Genet. 2015;49:213–242. doi:10.1146/annurev-genet-120213-092023
   PubMed Web of Science ®Google Scholar
• Costello MF, Misso ML, Balen A, et al. Evidence summaries and recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome: assessment and treatment of infertility. Hum Reprod Open. 2019;2019(1):hoy021. doi:10.1093/hropen/hoy021
   PubMedGoogle Scholar
• Gibson-Helm M, Teede H, Dunaif A, Dokras A. Delayed diagnosis and a lack of information associated with dissatisfaction in women with polycystic ovary syndrome. J Clin Endocrinol Metab. 2017;102(2):604–612.
   PubMed Web of Science ®Google Scholar
• Zhang C, Liu J, Lai M, et al. Circular RNA expression profiling of granulosa cells in women of reproductive age with polycystic ovary syndrome. Arch Gynecol Obstet. 2019;300(2):431–440. doi:10.1007/s00404-019-05129-5
   PubMed Web of Science ®Google Scholar
• Xu Y, Xu X, Ocansey DKW, et al. CircRNAs as promising biomarkers of inflammatory bowel disease and its associated-colorectal cancer. Am J Transl Res. 2021;13(3):1580–1593.
   PubMed Web of Science ®Google Scholar
• Yang T, Li Y, Zhao F, Zhou L, Jia R. Circular RNA Foxo3: a promising cancer-associated biomarker. Front Genet. 2021;12:652995. doi:10.3389/fgene.2021.652995
   PubMed Web of Science ®Google Scholar
• Liu Y, Li J, Bu H, et al. Circular RNA expression alteration identifies a novel circulating biomarker in serum exosomal for detection of alcohol dependence. Addict Biol. 2021;e13031. doi:10.1111/adb.13031
   PubMed Web of Science ®Google Scholar
• Jia C, Wang S, Yin C, Liu L, Zhou L, Ma Y. Loss of hsa_circ_0118530 inhibits human granulosa-like tumor cell line KGN cell injury by sponging miR-136. Gene. 2020;744:144591. doi:10.1016/j.gene.2020.144591
   PubMed Web of Science ®Google Scholar
• Wang J, Wu X. The effects of mitochondrial dysfunction on energy metabolism switch by HIF-1alpha signalling in granulosa cells of polycystic ovary syndrome. Endokrynol Pol. 2020;71(2):134–145. doi:10.5603/EP.a2020.0002
   PubMed Web of Science ®Google Scholar
• Zhao D, Qu Q, Dai H, et al. Effects of hypoxia-inducible factor‑1α on endometrial receptivity of women with polycystic ovary syndrome. Mol Med Rep. 2018;17(1):414–421.
   PubMed Web of Science ®Google Scholar
• Zheng R, Qing P, Han M, et al. The effect of acupuncture on glucose metabolism and lipid profiles in patients with PCOS: a systematic review and meta-analysis of randomized controlled trials. Evid Based Complement Alternat Med. 2021;2021:5555028. doi:10.1155/2021/5555028
   PubMed Web of Science ®Google Scholar
• Neubronner SA, Indran IR, Chan YH, Thu AWP, Yong EL. Effect of Body Mass Index (BMI) on phenotypic features of Polycystic Ovary Syndrome (PCOS) in Singapore women: a prospective cross-sectional study. BMC Womens Health. 2021;21(1):135. doi:10.1186/s12905-021-01277-6
   PubMed Web of Science ®Google Scholar
• Burbach BJ, O’Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021;12(1):3862. doi:10.1038/s41467-021-24132-6
   PubMed Web of Science ®Google Scholar
• Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi PP. A ceRNA hypothesis: the Rosetta stone of a hidden RNA language? Cell. 2011;146(3):353–358. doi:10.1016/j.cell.2011.07.014
   PubMed Web of Science ®Google Scholar
• Su ZH, Liao HH, Lu KE, et al. Hypoxia-responsive miR-346 promotes proliferation, migration, and invasion of renal cell carcinoma cells via targeting NDRG2. Neoplasma. 2020;67(5):1002–1011. doi:10.4149/neo_2020_190917N915
   PubMed Web of Science ®Google Scholar
• Kempinska-Podhorodecka A, Blatkiewicz M, Wunsch E, et al. Oncomir MicroRNA-346 is upregulated in colons of patients with primary sclerosing cholangitis. Clin Transl Gastroenterol. 2020;11(1):e00112. doi:10.14309/ctg.0000000000000112
   PubMed Web of Science ®Google Scholar