Advances in Diagnosis of Schistosomiasis: Focus on Challenges and Future Approaches

References

• Steinmann P, Keiser J, Bos R, et al. Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. Lancet Infect Dis. 2006;6(7):411–425. doi:10.1016/S1473-3099(06)70521-7
   PubMed Web of Science ®Google Scholar
• Bangert M, Molyneux DH, Lindsay SW, et al. The cross-cutting contribution of the end of neglected tropical diseases to the sustainable development goals. Infect Dis Poverty. 2017;6(1):73. doi:10.1186/s40249-017-0288-0
   PubMed Web of Science ®Google Scholar
• Gryseels B. Schistosomiasis. Infect Dis Clin North Am. 2012;26:383–397.
   PubMed Web of Science ®Google Scholar
• World Health Organization. Schistosomiasis Fact Sheet 115. Geneva, Switzerland: World Health Organization; 2014. Available from: http://www.who.int/mediacentre/factsheets/fs115/en/.2014. Accessed March 8, 2023.
   Google Scholar
• Ross AGP, Bartley PB, Sleigh AC, et al. Schistosomiasis. N Eng J Med. 2002;346:1212–1219.
   PubMed Web of Science ®Google Scholar
• Peters PA, Warren KS, Mahmoud AA. Rapid, accurate quantification of schistosome eggs via nuclepore filters. J Parasitol. 1976;62:154–155.
   PubMed Web of Science ®Google Scholar
• Kittur N, Binder S, Campbell CH, et al. Defining persistent hotspots: areas that fail to decrease meaningfully in prevalence after multiple years of mass drug administration with praziquantel for control of schistosomiasis. Am J Trop Med Hyg. 2017;97:1810–1817.
   PubMed Web of Science ®Google Scholar
• Wiegand RE, Mwinzi PN, Montgomery SP, et al. A persistent hotspot of Schistosoma mansoni infection in a five-year randomized trial of praziquantel preventative chemotherapy strategies. J Infect Dis. 2017;216:1425–1433.
   PubMed Web of Science ®Google Scholar
• CH King, Mahmoud AA. Drugs five years later: praziquantel. Ann Intern Med. 1989;110:290–296.
   PubMed Web of Science ®Google Scholar
• Ross AGP, Olveda RM, Acosta L, et al. Road to the elimination of schistosomiasis from Asia: the journey is far from over. Microbes Infect. 2013;15:858–865. doi:10.1016/j.micinf.2013.07.010
   PubMed Web of Science ®Google Scholar
• Stothard JR, Stanton MC, Bustinduy AL, et al. Diagnostics for schistosomiasis in Africa and Arabia: a review of present options in control and future needs for elimination. Parasitol. 2014;141:1947–1961. doi:10.1017/S0031182014001152
   PubMed Web of Science ®Google Scholar
• Weerakoon KGAD, Gobert GN, Cai P, McManus DP. Advances in the diagnosis of human Schistosomiasis. Clin Microbiol Rev. 2015;28:939–967. doi:10.1128/CMR.00137-14
   PubMed Web of Science ®Google Scholar
• Yumin Z, Weifeng G, Joseph WM, et al. Advances in diagnosis of schistosomiasis. Microbiol Curr Res. 2018;2(2):14–19.
   Google Scholar
• Hudu SA, Umar S. An overview of Schistosomiasis in Africa: evolutional origin and diagnostic challenges. Asian J Med Health. 2019;17(2):1–12.
   Google Scholar
• McManus DP, Dunne DW, Sacko M, et al. Schistosomiasis. Nat Rev Dis Primers. 2018;4(1):13. doi:10.1038/s41572-018-0013-8
   PubMed Web of Science ®Google Scholar
• Utzinger J, Becker SL, van Lieshout L, et al. New diagnostic tools in schistosomiasis. Clin Microbiol Infect. 2015;21(6):529–542. doi:10.1016/j.cmi.2015.03.014
   PubMed Web of Science ®Google Scholar
• Zhou YB, Zheng HM, Jiang QW. A diagnostic challenge for Schistosomiasis japonica in China: consequences on praziquantel-based morbidity control. Parasit Vectors. 2011;4:194.
   PubMed Web of Science ®Google Scholar
• Peters PA, El Alamy M, Warren KS, et al. Quick Kato smear for field quantification of Schistosoma mansoni eggs. Am J Trop Med Hyg. 1980;29:217–219.
   PubMed Web of Science ®Google Scholar
• Gryseels B, Polman K, Clerinx J, Kestens L. Human schistosomiasis. Lancet. 2006;368(9541):1106–1118.
   PubMed Web of Science ®Google Scholar
• World Health Organization. Schistosomiasis: Progress Report 2001–2011 and Strategic Plan 2012–2020. Geneva, Switzerland: World Health Organization; 2013.
   Google Scholar
• Katz N, Chaves A, Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 1972;14:397–400.
   PubMedGoogle Scholar
• Zhou YB, Yang MX, Wang QZ, et al. Field comparison of immunodiagnostic and parasitological techniques for the detection of Schistosomiasis japonica in the People’s Republic of China. Am J Trop Med Hyg. 2007;76:1138–1143.
   PubMed Web of Science ®Google Scholar
• Yu JM, de Vlas SJ, Jiang QW, et al. Comparison of the Kato-Katz technique, hatching test and indirect hemagglutination assay (IHA) for the diagnosis of Schistosoma japonicum infection in China. Parasitol Int. 2007;56:45–49. doi:10.1016/j.parint.2006.11.002
   PubMed Web of Science ®Google Scholar
• Lier T, Johansen MV, Hjelmevoll SO, et al. Real-time PCR for detection of low intensity Schistosoma japonicum infections in a pig model. Acta Trop. 2008;105:74–80. doi:10.1016/j.actatropica.2007.10.004
   PubMed Web of Science ®Google Scholar
• Yu JM, de Vlas SJ, Yuan HC, Gryseels B. Variations in fecal Schistosoma japonicum egg counts. Am J Trop Med Hyg. 1998;59:370–375.
   PubMed Web of Science ®Google Scholar
• Kongs A, Marks G, Verlé P, et al. The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Trop Med Int Health. 2001;6:163–169. doi:10.1046/j.1365-3156.2001.00687.x
   PubMed Web of Science ®Google Scholar
• Spear RC, Seto EYW, Carlton EJ, et al. The challenge of effective surveillance in moving from low transmission to elimination of schistosomiasis in China. Int J Parasitol. 2011;41:1243–1247. doi:10.1016/j.ijpara.2011.08.002
   PubMed Web of Science ®Google Scholar
• Degarege A, Legesse M, Medhin G, et al. Day-to-day fluctuation of point-of-care circulating cathodic antigen test scores and faecal egg counts in children infected with Schistosoma mansoni in Ethiopia. BMC Infect Dis. 2014;14:210. doi:10.1186/1471-2334-14-210
   PubMed Web of Science ®Google Scholar
• Glinz D, Silué KD, Knopp S, et al. Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths. PLoS Negl Trop Dis. 2010;4:e754. doi:10.1371/journal.pntd.0000754
   PubMed Web of Science ®Google Scholar
• Zhu H, Xu J, Zhu R, et al. Comparison of the miracidium hatching test and modified Kato-Katz method for detecting Schistosoma japonicum in low prevalence areas of China. Southeast Asian J Trop Med Public Health. 2014;45:20–25.
   PubMed Web of Science ®Google Scholar
• Jurberg AD, Oliveira AA, Lenzi HL, et al. A new miracidia hatching device for diagnosing schistosomiasis. MemInst Oswaldo Cruz. 2008;103:112–114. doi:10.1590/S0074-02762008005000005
   PubMed Web of Science ®Google Scholar
• Peters PA, Mahmoud AA, Warren KS, et al. Field studies of a rapid, accurate means of quantifying Schistosoma haematobium eggs in urine samples. Bull World Health Organ. 1976;54:159–162.
   PubMed Web of Science ®Google Scholar
• Stete K, Krauth SJ, Coulibaly JT, et al. Dynamics of Schistosoma haematobium egg output and associated infection parameters following treatment with praziquantel in school-aged children. Parasit Vectors. 2012;5:298. doi:10.1186/1756-3305-5-298
   PubMed Web of Science ®Google Scholar
• Wang C, Chen L, Yin X, et al. Application of DNA-based diagnostics in detection of schistosomal DNA in early infection and after drug treatment. Parasit Vectors. 2011;4:164. doi:10.1186/1756-3305-4-164
   PubMed Web of Science ®Google Scholar
• Bogoch II, Andrews JR, Dadzie Ephraim RK, et al. Simple questionnaire and urine reagent strips compared to microscopy for the diagnosis of Schistosoma haematobium in a community in northern Ghana. Trop Med Int Health. 2012;17:1217–1221. doi:10.1111/j.1365-3156.2012.03054.x
   PubMed Web of Science ®Google Scholar
• Emukah E, Gutman J, Eguagie JM, et al. Urine heme dipsticks are useful in monitoring the impact of praziquantel treatment on Schistosoma haematobium in sentinel communities of Delta State, Nigeria. Acta Trop. 2012;122:126–131. doi:10.1016/j.actatropica.2012.01.002
   PubMed Web of Science ®Google Scholar
• Doehring E, Vester U, Ehrich JHH. Circadian variation of ova excretion, proteinuria, hematuria and leukocyturia in urinary Schistosomiasis. Kidney Int. 1985;27(4):667–671.
   PubMed Web of Science ®Google Scholar
• Kamel B, Laidemitt MR, Lu L, et al. Detecting and identifying Schistosoma infections in snails and aquatic habitats: a systematic review. PLoS Negl Trop Dis. 2021;15(3):e0009175. doi:10.1371/journal.pntd.0009175
   PubMed Web of Science ®Google Scholar
• Gurarie D, King CH, Yoon N, et al. Seasonal dynamics of snail populations in coastal Kenya: model calibration and snail control. Adv Water Resour. 2017;108:397–405.
   Web of Science ®Google Scholar
• Abbasi I, King CH, Sturrock RF, et al. Differentiation of S. haematobium by PCR amplifying an inter-repeat sequence. Am J Trop Med Hyg. 2007;76(5):950–955.
   PubMed Web of Science ®Google Scholar
• Abath FG, Gomes ALDV, Melo FL, et al. Molecular approaches for the detection of Schistosoma mansoni: possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection. Mem Inst Oswaldo Cruz. 2006;101:145–148.
   PubMed Web of Science ®Google Scholar
• Bakuza JS, Gillespie R, Nkwengulila G, et al. Assessing S. mansoni prevalence in Biomphalaria snails in the Gombe ecosystem of western Tanzania: the importance of DNA sequence data for clarifying species identification. Parasites Vectors. 2017;10:584. doi:10.1186/s13071-017-2525-6
   PubMed Web of Science ®Google Scholar
• Opisa S, Odiere MR, Jura WG, et al. Malacological survey and geographical distribution of vector snails for schistosomiasis within informal settlements of Kisumu city, western Kenya. Parasit Vectors. 2011;4:226.
   PubMed Web of Science ®Google Scholar
• Odongo-Aginya EI, Kironde FK, Kabatereine NB, et al. Effect of seasonal rainfall and other environmental changes, on snail density and infection rates with schistosoma mansoni fifteen years after the last snails’ study in Kigungu, Entebbe, Uganda. East Afr Med J. 2008;85:556–563.
   PubMedGoogle Scholar
• Studer A, Poulin R. Seasonal dynamics in an intertidal mudflat: the case of a complex trematode life cycle. Mar Ecol Prog Ser. 2012;455:79–93.
   Web of Science ®Google Scholar
• Wu Z, Liang L, Jiang Q, et al. Case of intracranial schistosomiasis granuloma was misdiagnosed as brain metastases. Chin J Clin Neurosurg. 2017;22:286.
   Google Scholar
• Alarcón de Noya B, Ruiz R, Losada S, et al. Detection of schistosomiasis cases in low-transmission areas based on coprologic and serologic criteria. The Venezuelan experience. Acta Trop. 2007;103:41–49. doi:10.1016/j.actatropica.2007.04.018
   PubMed Web of Science ®Google Scholar
• Doenhoff MJ, Chiodini PL, Hamilton JV. Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies?Trends. Parasitol. 2004;20(1):35–39.
   Web of Science ®Google Scholar
• Wang L, Utzinger J, Zhou XN. Schistosomiasis control: experiences and lessons from China. Lancet. 2008;372(9652):1793–1795.
   PubMed Web of Science ®Google Scholar
• Othman RA. Indirect haemagglutination test and ELISA as compared to Kato thick-smear in diagnosing Schistosoma mansoni. J Egypt Soc Parasitol. 2013;43:841–848.
   PubMedGoogle Scholar
• Kahama AI, Kremsner PG, van Dam GJ, et al. The dynamics of a soluble egg antigen of Schistosoma haematobium in relation to egg counts, circulating anodic and cathodic antigens and pathology markers before and after chemotherapy. Trans R Soc Trop Med Hyg. 1998;92:629–633.
   PubMed Web of Science ®Google Scholar
• Mahfouz A, Mahana N, Rabee I. Evaluation of different immunological techniques for diagnosis of Schistosomiasis haematobium in Egypt. Life Sci J. 2011;8:4.
   Google Scholar
• El-Morshedy H, Kinosien B, Barakat R, et al. Circulating anodic antigen for detection of Schistosoma mansoni infection in Egyptian patients. Am J Trop Med Hyg. 1996;54:149–153.
   PubMed Web of Science ®Google Scholar
• Grenfell R, Martins W, Silva-Moraes V, et al. The schistosomula tegument antigen as a potential candidate for the early serological diagnosis of schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 2013;55(2):75–78.
   PubMed Web of Science ®Google Scholar
• Carvalho GB, Pacífico LG, Pimenta DL, et al. Evaluation of the use of C-terminal part of the Schistosoma mansoni 200kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation. Exp Parasitol. 2014;139:24–32. doi:10.1016/j.exppara.2014.02.003
   PubMed Web of Science ®Google Scholar
• De Oliveira EJ, Kanamura HY, Correia lima DM. Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden. Mem Inst Oswaldo Cruz. 2005;100:421–425. doi:10.1590/S0074-02762005000400013
   PubMed Web of Science ®Google Scholar
• Zhou YB, Yang MX, Tao P, et al. A longitudinal study of comparison of the Kato-Katz technique and indirect hemagglutination assay (IHA) for the detection of schistosomiasis japonica in China, 2001–2006. Acta Trop. 2008;107(3):251–254.
   PubMed Web of Science ®Google Scholar
• Zhang YY, Luo JP, Liu YM, et al. Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop. 2009;112:16–22.
   PubMed Web of Science ®Google Scholar
• Gray DJ, Ross AG, Li YS, et al. Diagnosis and management of schistosomiasis. BMJ. 2011;342:d2651.
   PubMed Web of Science ®Google Scholar
• Jauréguiberry S, Paris L, Caumes E. Acute schistosomiasis, a diagnostic and therapeutic challenge. Clin Microbiol Infect Dis. 2010;16:225–231. doi:10.1111/j.14690691.2009.03131.x
   Google Scholar
• Weerakoon KG, McManus DP. Cell-Free DNA as a Diagnostic tool for human parasitic infections. Trends Parasitol. 2016;32:378–391. doi:10.1016/j.pt.2016.01.006
   PubMed Web of Science ®Google Scholar
• Montresor A, Crompton DWT, Hall A, et al. Guidelines for the Evaluation of Soil-Transmitted Helminthiasis and Schistosomiasis at Community Level: A Guide for Managers of Control Programmes. Geneva: World Health Organization; 1998.
   Google Scholar
• Xia CM, Rong R, Lu ZX, et al. Schistosoma japonicum: a PCR assay for the early detection and evaluation of treatment in a rabbit model. Exp Parasitol. 2009;121:175–179.
   PubMed Web of Science ®Google Scholar
• Tsang VC, Hillyer GV, Noh J, et al. Geographic clustering and seroprevalence of schistosomiasis in Puerto Rico (1995). Am J Trop Med Hyg. 1997;56(1):107–112.
   PubMed Web of Science ®Google Scholar
• Zhu Y, Hua W, Xu M, et al. A novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from Schistosoma japonicum. PLoS One. 2012;7:e44032. doi:10.1371/journal.pone.0044032
   PubMed Web of Science ®Google Scholar
• Shiff C. New diagnostics reform infectious parasite epidemiology. Lancet Infect Dis. 2014;14:446–448. doi:10.1016/S1473-3099(14)70707-8
   PubMed Web of Science ®Google Scholar
• Ibironke O, Koukounari A, Asaolu S, et al. Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. PLoS Negl Trop Dis. 2012;6:e1464. doi:10.1371/journal.pntd.0001464
   PubMed Web of Science ®Google Scholar
• Wichmann D, Poppert S, Von Thien H, et al. Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis. BMC Infect Dis. 2013;30(13):55.
   Google Scholar
• Allan F, Dunn AM, Emery AM, et al. Use of sentinel snails for the detection of Schistosoma haematobium transmission on Zanzibar and observations on transmission patterns. Acta Trop. 2013;128:234–240.
   PubMed Web of Science ®Google Scholar
• Aryeetey YA, Essien-Baidoo S, Larbi IA, et al. Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana. Am J Trop Med Hyg. 2013;88:1028–1031.
   PubMed Web of Science ®Google Scholar
• Ibironke OA, Phillips AE, Garba A, et al. Diagnosis of Schistosoma haematobium by detection of specific DNA fragments from filtered urine samples. Am J Trop Med Hyg. 2011;84:998–1001. doi:10.4269/ajtmh.2011.10-0691
   PubMed Web of Science ®Google Scholar
• Pai NP, Vadnais C, Denkinger C, et al. Point-of-care testing for infectious diseases: diversity, complexity, and barriers in low- and middle-income countries. PLoS Med. 2012;9:e1001306.
   PubMed Web of Science ®Google Scholar
• Vinkeles Melchers NVS, van Dam GJ, Shaproski D, et al. Diagnostic performance of Schistosoma real-time PCR in urine samples from Kenyan children infected with Schistosoma haematobium: day-to-day variation and follow-up after praziquantel treatment. PLoS Negl Trop Dis. 2014;8:e2807.
   PubMed Web of Science ®Google Scholar
• Hussein HM, El-Tonsy MM, Tawfik RA, et al. Experimental study for early diagnosis of prepatent schistosomiasis mansoni by detection of free circulating DNA in serum. Parasitol Res. 2012;111:475–478.
   PubMed Web of Science ®Google Scholar
• Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63. doi:10.1093/nar/28.12.e63
   PubMed Web of Science ®Google Scholar
• Liu F, Lu J, Hu W, et al. New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. PLoSPathog. 2006;2:e29. doi:10.1371/journal.ppat.0020029
   Web of Science ®Google Scholar
• Zhang M, Fu Z, Li C, et al. Screening diagnostic candidates for schistosomiasis from tegument proteins of adult schistosoma japonicum using an immunoproteomic approach. PLoS Negl Trop Dis. 2015;9(2):e0003454.
   PubMed Web of Science ®Google Scholar
• TchuemTchuenté LA, Rollinson D, Stothard JR, et al. Moving from control to elimination of schistosomiasis in sub-Saharan Africa: time to change and adapt strategies. Infect Dis Poverty. 2017;6:42. doi:10.1186/s40249-017-0256-8
   PubMed Web of Science ®Google Scholar
• Wu Y, Jiaqing L, Lin Y, et al. Diagnosis, monitoring, and control of schistosomiasis—an update. J Biomed Nanotechnol. 2018;14(3):430–455. doi:10.1166/jbn.2018.2517
   PubMed Web of Science ®Google Scholar
• Sun B. Diagnostic value of liver ultrasound in schistosomiasis. Health Way. 2016;15:252–253.
   Google Scholar
• Fernández-Soto P, Gandasegui J, Carranza Rodríguez C, et al. Detection of Schistosoma mansoni-derived DNA in human urine samples by loop-mediated isothermal amplification (LAMP). PLoS One. 2019;14(3):e0214125. doi:10.1371/journal.pone.0214125
   PubMed Web of Science ®Google Scholar
• Kato-Hayashi N, Leonardo LR, Arevalo NL, et al. Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines. Acta Trop. 2015;141:178–183. doi:10.1016/j.actatropica.2014.05.003
   PubMed Web of Science ®Google Scholar
• Gomes LI, Dos Santos Marques LH, Enk MJ, et al. Development and evaluation of a sensitive PCR-ELISA system for detection of Schistosoma infection in feces. PLoS Negl Trop Dis. 2010;4:e664.
   PubMed Web of Science ®Google Scholar
• Caliendo AM. Multiplex PCR and emerging technologies for the detection of respiratory pathogens. Clin Infect Dis. 2011;2:326–330.
   Google Scholar
• Thekisoe OM, Kuboki N, Nambota A, et al. Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis. Acta Trop. 2007;102:182–189.
   PubMed Web of Science ®Google Scholar
• Lodh N, Mikita K, Bosompem KM, et al. Species-specific DNA detection in urine by loop-mediated isothermal amplification (LAMP). Acta Trop. 2017;173:125–129.
   PubMed Web of Science ®Google Scholar
• Tomita N, Mori Y, Kanda H, et al. Loop‐mediated isothermal amplification (lamp) of gene sequences and simple visual detection of products. Nat Protoc. 2008;3:877–882. doi:10.1038/nprot.2008.57
   PubMed Web of Science ®Google Scholar
• Tanner NA, Zhang Y, Evans TC. Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. Biotechniques. 2012;53:81–89. doi:10.2144/0000113902
   PubMed Web of Science ®Google Scholar
• Rosser A, Rollinson D, Forrest M, et al. Isothermal recombinase polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection. Parasit Vectors. 2015;8:446. doi:10.1186/s13071-015-1055-3
   PubMed Web of Science ®Google Scholar
• Xing W, Yu X, Feng J, et al. Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China. BMC Infect Dis. 2017;17:164. doi:10.1186/s12879-017-2182-6
   PubMed Web of Science ®Google Scholar
• van Lieshout L, Roestenberg M. Clinical consequences of new diagnostic tools for intestinal parasites. Clin Microbiol Infect. 2015;21:520–528. doi:10.1016/j.cmi.2015.03.015
   PubMed Web of Science ®Google Scholar
• Ajibola O, Gulumbe BH, Eze AA, et al. Tools for detection of schistosomiasis in resource limited settings. Med Sci Basel. 2018;6(2):39. PMID: 29789498; PMCID: PMC6024580. doi:10.3390/medsci6020039
   Google Scholar
• van Grootveld R, van Dam GJ, de Dood CJ, et al. Improved diagnosis of active Schistosoma infection in travelers and migrants using the ultra-sensitive in-house lateral flow test for detection of circulating anodic antigen (CAA) in serum. Eur J Clin Microbiol Infect Dis. 2018;37(9):1709–1716.
   PubMed Web of Science ®Google Scholar
• Utzinger J, N’goran EK, Caffrey CR, et al. From innovation to application: social-ecological context, diagnostics, drugs and integrated control of schistosomiasis. Acta Trop. 2011;120:121–137.
   PubMed Web of Science ®Google Scholar
• Peeling RW, Nwaka S. Drugs and diagnostic innovations to improve global health. Infect Dis Clin North Am. 2011;25:693–705.
   PubMed Web of Science ®Google Scholar
• Mabey D, Peeling R, Ustianowski A, et al. Diagnostics for the developing world. Nat Rev Microbiol. 2004;2:231–240. doi:10.1038/nrmicro84
   PubMed Web of Science ®Google Scholar
• Worrell CM, Bartoces M, Karanja DMS, et al. Cost analysis of tests for the detection of Schistosoma mansoni infection in children in Western Kenya. Am J Trop Med Hyg. 2015;92:1233–1239. doi:10.4269/ajtmh.14-0644
   PubMed Web of Science ®Google Scholar
• Weerakoon K, Gordon C, McManus DP. DNA diagnostics for schistosomiasis control. Trop Med Infect Dis. 2018;3:81.
   PubMedGoogle Scholar
• Rollinson D, Knopp S, Levitz S, et al. Time to set the agenda for schistosomiasis elimination. Acta Trop. 2013;128:423–440.
   PubMed Web of Science ®Google Scholar
• Mbabazi PS, Vwalika B, Randrianasolo BS, et al. World Health Organisation Female Genital Schistosomiasis. A Pocket Atlas for Clinical Health-Care Professionals. Geneva: World Health Organisation; 2015:49. Available from: http://apps.who.int/iris/bitstream/10665/180863/1/9789241509299_eng.pdf. Accessed March 8, 2023.
   Google Scholar
• Sturt AS, Webb EL, Phiri CR, et al. Genital self-sampling compared with cervicovaginal lavage for the diagnosis of female genital schistosomiasis in Zambian women: the BILHIV study. PLoS Negl Trop Dis. 2020;14(7):e0008337. doi:10.1371/journal.pntd.0008337
   PubMed Web of Science ®Google Scholar