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REVIEW

Chloride Channel and Inflammation-Mediated Pathogenesis of Osteoarthritis

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Pages 953-964 | Published online: 11 Feb 2022

References

  • Barrett-Jolley R, Lewis R, Fallman R, et al. The emerging chondrocyte channelome. Front Physiol. 2010;1:135. doi:10.3389/fphys.2010.00135
  • Oláh T, Cai X, Michaelis JC, et al. Comparative anatomy and morphology of the knee in translational models for articular cartilage disorders. Part I: large animals. Ann Anat. 2021;235:151680.
  • Yamamura H, Suzuki Y, Imaizumi Y. Physiological and pathological functions of Cl channels in chondrocytes. Biol Pharm Bull. 2018;41(8):1145–1151. doi:10.1248/bpb.b18-00152
  • Hodgkinson T, Kelly DC, Curtin CM, et al. Mechanosignalling in cartilage: an emerging target for the treatment of osteoarthritis. Nat Rev Rheumatology. 2021;3:1–18.
  • Gilbert SJ, Bonnet CS, Blain EJ. Mechanical cues: bidirectional reciprocity in the extracellular matrix drives mechano-signalling in articular cartilage. Int J Mol Sci. 2021;22(24):13595. doi:10.3390/ijms222413595
  • Liu Y, Shah KM, Luo J. Strategies for articular cartilage repair and regeneration. Front Bioeng Biotechnol. 2021;9:770655. doi:10.3389/fbioe.2021.770655
  • Deng J, Zong Z, Su Z, et al. Recent advances in pharmacological intervention of osteoarthritis: a biological aspect. Front Pharmacol. 2021;12. doi:10.3389/fphar.2021.772678
  • Hunter DJ, Bierma-Zeinstra S. Osteoarthritis. Lancet. 2019;393(10182):1745–1759. doi:10.1016/S0140-6736(19)30417-9
  • Hunter DJ, Schofield D, Callander E. The individual and socioeconomic impact of osteoarthritis. Nat Rev Rheumatol. 2014;10(7):437–441. doi:10.1038/nrrheum.2014.44
  • Vos T, Flaxman AD, Naghavi M, et al. Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet. 2012;380(9859):2163–2196.
  • Garcia G, Martínez-Rojas VA, Rocha-González HI, et al. Evidence for the participation of Ca(2+)-activated chloride channels in formalin-induced acute and chronic nociception. Brain Res. 2014;1579:35–44. doi:10.1016/j.brainres.2014.07.011
  • Ebbinghaus M, Gajda M, Holtzman MJ, et al. Does chloride channel accessory 3 have a role in arthritis pain? A study on murine antigen-induced arthritis. Neurosci Lett. 2014;576:40–44. doi:10.1016/j.neulet.2014.05.051
  • Jentsch T, Stein V, Weinreich F, et al. Molecular structure and physiological function of chloride channels. Physiol Rev. 2002;82(2):503–568. doi:10.1152/physrev.00029.2001
  • Smith KR, Kittler JT. The cell biology of synaptic inhibition in health and disease. Curr Opin Neurobiol. 2010;20(5):550–556. doi:10.1016/j.conb.2010.06.001
  • Mohler H, Malherbe P, Draguhn A, et al. GABAA-receptors: structural requirements and sites of gene expression in mammalian brain. Neurochem Res. 1990;15(2):199–207. doi:10.1007/BF00972210
  • Zhang H, Deng Z, Yang L, et al. The AQP-3 water channel is a pivotal modulator of glycerol-induced chloride channel activation in nasopharyngeal carcinoma cells. Int J Biochem Cell Biol. 2016;72:89–99. doi:10.1016/j.biocel.2016.01.009
  • Deng Z, Li W, Xu J, et al. ClC-3 chloride channels are involved in estradiol regulation of bone formation by MC3T3-E1 osteoblasts. J Cell Biochem. 2018;120:8366–8375.
  • Deng Z, Peng S, Zheng Y, et al. Estradiol activates chloride channels via estrogen receptor-α in the cell membranes of osteoblasts. Am J Physiol Cell Physiol. 2017;313(2):C162–c172. doi:10.1152/ajpcell.00014.2017
  • Bertram KL, Krawetz RJ. Osmolarity regulates chondrogenic differentiation potential of synovial fluid derived mesenchymal progenitor cells. Biochem Biophys Res Commun. 2012;422(3):455–461. doi:10.1016/j.bbrc.2012.05.015
  • Nguyen TD, Oloyede A, Singh S, et al. Investigation of the effects of extracellular osmotic pressure on morphology and mechanical properties of individual chondrocyte. Cell Biochem Biophys. 2016;74(2):229–240. doi:10.1007/s12013-016-0721-1
  • Johnson ZI, Shapiro IM, Risbud MV. Extracellular osmolarity regulates matrix homeostasis in the intervertebral disc and articular cartilage: evolving role of TonEBP. Matrix Biol. 2014;40:10–16. doi:10.1016/j.matbio.2014.08.014
  • Wang M, Yang Y, Han L, et al. Cell mechanical microenvironment for cell volume regulation. J Cell Physiol. 2020;235(5):4070–4081. doi:10.1002/jcp.29341
  • Yamada S, Suzuki Y, Bernotiene E, et al. Swelling-activated ClC-3 activity regulates prostaglandin E release in human OUMS-27 chondrocytes. Biochem Biophys Res Commun. 2021;537:29–35. doi:10.1016/j.bbrc.2020.12.068
  • Moretti J, Blander JM. Increasing complexity of NLRP3 inflammasome regulation. J Leukoc Biol. 2021;109(3):561–571.
  • Wang Z, Irianto J, Kazun S, et al. The rate of hypo-osmotic challenge influences regulatory volume decrease (RVD) and mechanical properties of articular chondrocytes. Osteoarthr Cartil. 2015;23(2):289–299. doi:10.1016/j.joca.2014.11.003
  • Komori T. Cell death in chondrocytes, osteoblasts, and osteocytes. Int J Mol Sci. 2016;17(12):2045. doi:10.3390/ijms17122045
  • Kittl M, Winklmayr M, Helm K, et al. Acid- and volume-sensitive chloride currents in human chondrocytes. Front Cell Dev Biol. 2020;8:583131. doi:10.3389/fcell.2020.583131
  • Hall A. The role of chondrocyte morphology and volume in controlling phenotype-implications for osteoarthritis, cartilage repair, and cartilage engineering. Curr Rheumatol Rep. 2019;21(8):38. doi:10.1007/s11926-019-0837-6
  • Ponce A, Jimenez-Peña L, Tejeda-Guzman C. The role of swelling-activated chloride currents (I(CL, swell)) in the regulatory volume decrease response of freshly dissociated rat articular chondrocytes. Cell Physiol Biochem. 2012;30(5):1254–1270.
  • Riemma G, Laganà AS, Schiattarella A, et al. Ion channels in the pathogenesis of endometriosis: a cutting-edge point of view. Int J Mol Sci. 2020;21(3):1114. doi:10.3390/ijms21031114
  • Kurita T, Yamamura H, Suzuki Y, et al. The ClC-7 chloride channel is downregulated by hypoosmotic stress in human chondrocytes. Mol Pharmacol. 2015;88(1):113–120. doi:10.1124/mol.115.098160
  • Voss FK, Ullrich F, Münch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science. 2014;344(6184):634–638. doi:10.1126/science.1252826
  • Chen L, König B, Liu T, et al. More than just a pressure relief valve: physiological roles of volume-regulated LRRC8 anion channels. Biol Chem. 2019;400(11):1481–1496.
  • Liu T, Stauber T. The volume-regulated anion channel LRRC8/VRAC is dispensable for cell proliferation and migration. Int J Mol Sci. 2019;20(11):453.
  • Zhang H, Deng Z, Zhang D, et al. High expression of leucine‑rich repeat‑containing 8A is indicative of a worse outcome of colon cancer patients by enhancing cancer cell growth and metastasis. Oncol Rep. 2018;40(3):1275–1286.
  • Compan V, Baroja-Mazo A, López-Castejón G, et al. Cell volume regulation modulates NLRP3 inflammasome activation. Immunity. 2012;37(3):487–500. doi:10.1016/j.immuni.2012.06.013
  • Deng Z, Lin Z, Zhong Q, et al. Interleukin 1 beta-induced chloride currents are important in osteoarthritis onset: an in vitro study. Acta Biochim Biophys Sin (Shanghai). 2021;53(4):400–409. doi:10.1093/abbs/gmab010
  • Vaca-González J, Guevara JM, Moncayo MA, et al. Biophysical stimuli: a review of electrical and mechanical stimulation in hyaline cartilage. Cartilage. 2019;10(2):157–172. doi:10.1177/1947603517730637
  • Nguyen TH, Duong CM, Nguyen X-H, et al. Mesenchymal stem cell-derived extracellular vesicles for osteoarthritis treatment: extracellular matrix protection, chondrocyte and osteocyte physiology, pain and inflammation management. Cells. 2021;10(11):2887. doi:10.3390/cells10112887
  • Park S, Bello A, Arai Y, et al. Functional duality of chondrocyte hypertrophy and biomedical application trends in osteoarthritis. Pharmaceutics. 2021;13(8):1139. doi:10.3390/pharmaceutics13081139
  • Zhang H, Wei Q-F, Wang S-J, et al. LncRNA HOTAIR alleviates rheumatoid arthritis by targeting miR-138 and inactivating NF-κB pathway. Int Immunopharmacol. 2017;50:283–290. doi:10.1016/j.intimp.2017.06.021
  • Xie Q, Wei M, Kang X, et al. Reciprocal inhibition between miR-26a and NF-κB regulates obesity-related chronic inflammation in chondrocytes. Biosci Rep. 2015;35(3). doi:10.1042/BSR20150071
  • Duan L, Liang Y, Ma B, et al. Epigenetic regulation in chondrocyte phenotype maintenance for cell-based cartilage repair. Am J Transl Res. 2015;7(11):2127–2140.
  • Duan L, Ma B, Liang Y, et al. Cytokine networking of chondrocyte dedifferentiation in vitro and its implications for cell-based cartilage therapy. Am J Transl Res. 2015;7(2):194–208.
  • Hsu KS, Otsu W, Li Y, et al. CLIC4 regulates late endosomal trafficking and matrix degradation activity of MMP14 at focal adhesions in RPE cells. Sci Rep. 2019;9(1):12247. doi:10.1038/s41598-019-48438-0
  • Guan Y, Luan Y, Xie Y, et al. Chloride channel-3 is required for efficient tumour cell migration and invasion in human cervical squamous cell carcinoma. Gynecol Oncol. 2019;153(3):661–669. doi:10.1016/j.ygyno.2019.03.006
  • Wang B, Xie J, He H-Y, et al. Suppression of CLC-3 chloride channel reduces the aggressiveness of glioma through inhibiting nuclear factor-κB pathway. Oncotarget. 2017;8(38):63788–63798. doi:10.18632/oncotarget.19093
  • Chung H, Wen Y, Chun S-H, et al. Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 mRNA expression in ectopic and eutopic endometrium in women with endometriosis: a rationale for endometriotic invasiveness. Fertil Steril. 2001;75(1):152–159. doi:10.1016/S0015-0282(00)01670-8
  • Xin L, Hou Q, Xiong QI, et al. Association between matrix metalloproteinase-2 and matrix metalloproteinase-9 polymorphisms and endometriosis: a systematic review and meta-analysis. Biomed Rep. 2015;3(4):559–565. doi:10.3892/br.2015.447
  • Sun L, Dong Y, Zhao J, et al. The CLC-2 chloride channel modulates ECM synthesis, differentiation, and migration of human conjunctival fibroblasts via the PI3K/Akt signaling pathway. Int J Mol Sci. 2016;17(6):910. doi:10.3390/ijms17060910
  • Bostanci Durmus A, Dincer Cengiz S, Yılmaz H, et al. The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosis. J Obstet Gynaecol. 2019;39(7):991–995. doi:10.1080/01443615.2019.1584889
  • Chaisson C, Zhang Y, McAlindon TE, et al. Radiographic hand osteoarthritis: incidence, patterns, and influence of pre-existing disease in a population based sample. J Rheumatol. 1997;24(7):1337–1343.
  • Xiao YP, Tian F-M, Dai M-W, et al. Are estrogen-related drugs new alternatives for the management of osteoarthritis? Arthritis Res Ther. 2016;18:151. doi:10.1186/s13075-016-1045-7
  • Wilson MG, Michet CJ, Ilstrup DM, et al. Idiopathic symptomatic osteoarthritis of the hip and knee: a population-based incidence study. Mayo Clin Proc. 1990;65(9):1214–1221. doi:10.1016/S0025-6196(12)62745-1
  • Liu H, Yang S-D, Xu Y, et al. Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells. Mol Med Rep. 2017;16(2):1093–1100. doi:10.3892/mmr.2017.6690
  • Wang Y, Liu Z, Wang Q, et al. Early detection of tibial cartilage degradation and cancellous bone loss in an ovariectomized rat model. Biomed Res Int. 2017;2017:9654056.
  • Hu Q, Ecker M. Overview of MMP-13 as a promising target for the treatment of osteoarthritis. Int J Mol Sci. 2021;22(4):e12.
  • Liang Y, Duan L, Xiong J, et al. E2 regulates MMP-13 via targeting miR-140 in IL-1β-induced extracellular matrix degradation in human chondrocytes. Arthritis Res Ther. 2016;18(1):105. doi:10.1186/s13075-016-0997-y
  • He Y, Hara H, Nunez G. Mechanism and regulation of NLRP3 inflammasome activation. Trends Biochem Sci. 2016;41(12):1012–1021.
  • Tang T, Lang X, Xu C, et al. CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nat Commun. 2017;8(1):202. doi:10.1038/s41467-017-00227-x
  • Cullen SP, Kearney C, Clancy D, et al. Diverse activators of the NLRP3 inflammasome promote IL-1beta secretion by triggering necrosis. Cell Rep. 2015;11(10):1535–1548. doi:10.1016/j.celrep.2015.05.003
  • Lee GS, Subramanian N, Kim AI, et al. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature. 2012;492(7427):123–127. doi:10.1038/nature11588
  • Munoz-Planillo R, Kuffa P. K(+) efflux is the common trigger of NLRP3 inflammasome activation by bacterial toxins and particulate matter. Immunity. 2013;38(6):1142–1153. doi:10.1016/j.immuni.2013.05.016
  • Daniels MJ, Rivers-Auty J, Schilling T, et al. Fenamate NSAIDs inhibit the NLRP3 inflammasome and protect against Alzheimer’s disease in rodent models. Nat Commun. 2016;7:12504. doi:10.1038/ncomms12504
  • Son YO, Park S, Kwak J-S, et al. Estrogen-related receptor gamma causes osteoarthritis by upregulating extracellular matrix-degrading enzymes. Nat Commun. 2017;8(1):2133. doi:10.1038/s41467-017-01868-8
  • Chen C, Smith AD, Cheung L, et al. Potentiation of IL-4 signaling by retinoic acid in intestinal epithelial cells and macrophages-mechanisms and targets. Front Immunol. 2020;11:605. doi:10.3389/fimmu.2020.00605
  • Kim HJ, Nam YR, Nam JH. Flos magnoliae inhibits chloride secretion via ANO1 inhibition in Calu-3 cells. Am J Chin Med. 2018;46(5):1079–1092. doi:10.1142/S0192415X18500568
  • Green JP, Swanton T, Morris LV, et al. LRRC8A is essential for hypotonicity-, but not for DAMP-induced NLRP3 inflammasome activation. Elife. 2020;9. doi:10.7554/eLife.59704
  • Osei-Owusu J, Yang J, del Carmen Vitery M, et al. Molecular biology and physiology of volume-regulated anion channel (VRAC). Curr Top Membr. 2018;81:177–203.
  • Peng JM, Lin S-H, Yu M-C, et al. CLIC1 recruits PIP5K1A/C to induce cell-matrix adhesions for tumor metastasis. J Clin Invest. 2021;131(1). doi:10.1172/JCI133525
  • Cabrita I, Benedetto R, Wanitchakool P, et al. TMEM16A mediates mucus production in human airway epithelial cells. Am J Respir Cell Mol Biol. 2021;64(1):50–58.
  • Mertens TC, Hiemstra PS, Taube C. Azithromycin differentially affects the IL-13-induced expression profile in human bronchial epithelial cells. Pulm Pharmacol Ther. 2016;39:14–20. doi:10.1016/j.pupt.2016.05.005
  • Qin Y, Jiang Y, Sheikh AS, et al. Interleukin-13 stimulates MUC5AC expression via a STAT6-TMEM16A-ERK1/2 pathway in human airway epithelial cells. Int Immunopharmacol. 2016;40:106–114. doi:10.1016/j.intimp.2016.08.033
  • Bergsbaken T, Fink SL, Cookson BT. Pyroptosis: host cell death and inflammation. Nat Rev Microbiol. 2009;7(2):99–109. doi:10.1038/nrmicro2070
  • Zhang L, Xing R, Huang Z, et al. Inhibition of synovial macrophage pyroptosis alleviates synovitis and fibrosis in knee osteoarthritis. Mediators Inflamm. 2019;2019:2165918. doi:10.1155/2019/2165918
  • An S, Hu H, Li Y, et al. Pyroptosis plays a role in osteoarthritis. Aging Dis. 2020;11(5):1146–1157. doi:10.14336/AD.2019.1127
  • Borgonio Cuadra VM, González-Huerta NC, Romero-Córdoba S, et al. Altered expression of circulating microRNA in plasma of patients with primary osteoarthritis and in silico analysis of their pathways. PLoS One. 2014;9(6):e97690. doi:10.1371/journal.pone.0097690
  • Zhao LR, Xing RL, Wang PM, et al. NLRP1 and NLRP3 inflammasomes mediate LPS/ATPinduced pyroptosis in knee osteoarthritis. Mol Med Rep. 2018;17(4):5463–5469.
  • Domingo-Fernandez R, Coll RC, Kearney J, et al. The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1beta transcription and activate the NLRP3 inflammasome. J Biol Chem. 2017;292(29):12077–12087. doi:10.1074/jbc.M117.797126
  • Zhao K, An R, Xiang Q, et al. Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration. Cell Prolif. 2021;54(1):e12941. doi:10.1111/cpr.12941
  • Culemann S, Grüneboom A, Nicolás-Ávila JÁ, et al. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature. 2019;572(7771):670–675.
  • Qian J, Fu P, Li S, et al. miR-107 affects cartilage matrix degradation in the pathogenesis of knee osteoarthritis by regulating caspase-1. J Orthop Surg Res. 2021;16(1):40. doi:10.1186/s13018-020-02121-7
  • Zhang L, Qiu J, Shi J, et al. MicroRNA-140-5p represses chondrocyte pyroptosis and relieves cartilage injury in osteoarthritis by inhibiting cathepsin B/Nod-like receptor protein 3. Bioengineered. 2021;12(2):9949–9964.
  • Xu H, Xu B, Yokota S-I. BMSC-derived exosomes ameliorate osteoarthritis by inhibiting pyroptosis of cartilage via delivering miR-326 targeting HDAC3 and STAT1//NF-kappaB p65 to chondrocytes. Mediators Inflamm. 2021;2021:9972805. doi:10.1155/2021/9972805
  • Castelblanco M, Lugrin J, Ehirchiou D, et al. Hydrogen sulfide inhibits NLRP3 inflammasome activation and reduces cytokine production both in vitro and in a mouse model of inflammation. J Biol Chem. 2018;293(7):2546–2557. doi:10.1074/jbc.M117.806869
  • Wei Z, Zhan X, Ding K, et al. Dihydrotanshinone I specifically inhibits NLRP3 inflammasome activation and protects against septic shock in vivo. Front Pharmacol. 2021;12:750815.
  • Sarbadhikary P, George BP, Abrahamse H. Inhibitory role of berberine, an isoquinoline alkaloid, on NLRP3 inflammasome activation for the treatment of inflammatory diseases. Molecules. 2021;26(20):6238. doi:10.3390/molecules26206238
  • Liu Q, Wu Z, Hu D, et al. Low dose of indomethacin and Hedgehog signaling inhibitor administration synergistically attenuates cartilage damage in osteoarthritis by controlling chondrocytes pyroptosis. Gene. 2019;712:143959. doi:10.1016/j.gene.2019.143959
  • Zu Y, Mu Y, Li Q, et al. Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis. J Orthop Surg Res. 2019;14(1):307. doi:10.1186/s13018-019-1307-6
  • Hu J, Zhou J, Wu J, et al. Loganin ameliorates cartilage degeneration and osteoarthritis development in an osteoarthritis mouse model through inhibition of NF-kappaB activity and pyroptosis in chondrocytes. J Ethnopharmacol. 2020;247:112261. doi:10.1016/j.jep.2019.112261
  • Kumagai K, Toyoda F, Staunton CA, et al. Activation of a chondrocyte volume-sensitive Cl(-) conductance prior to macroscopic cartilage lesion formation in the rabbit knee anterior cruciate ligament transection osteoarthritis model. Osteoarthr Cartil. 2016;24(10):1786–1794. doi:10.1016/j.joca.2016.05.019
  • Zhou C, Tang X, Xu J, et al. Opening of the CLC-3 chloride channel induced by dihydroartemisinin contributed to early apoptotic events in human poorly differentiated nasopharyngeal carcinoma cells. J Cell Biochem. 2018;119(11):9560–9572. doi:10.1002/jcb.27274
  • Huang L, Li Y-J, Li -P-P, et al. Aggravated intestinal apoptosis by ClC-3 deletion is lethal to mice endotoxemia. Cell Biol Int. 2018;42(10):1445–1453. doi:10.1002/cbin.11025
  • Fan F, Liu T, Wang X, et al. ClC-3 expression and its association with hyperglycemia induced HT22 hippocampal neuronal cell apoptosis. J Diabetes Res. 2016;2016:2984380. doi:10.1155/2016/2984380
  • Bi M, Hong S, Ma LJ, et al. Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells. Iran J Basic Med Sci. 2016;19(7):705–711.
  • Deng Z, Li W, Alahdal M, et al. Overexpression of ClC-3 chloride channel in chondrosarcoma: an in vivo immunohistochemical study with tissue microarray. Med Sci Monit. 2019;25:5044–5053. doi:10.12659/MSM.917382
  • Driban JB, Hootman JM, Sitler MR, et al. Is participation in certain sports associated with knee osteoarthritis? a systematic review. J Athl Train. 2017;52(6):497–506. doi:10.4085/1062-6050-50.2.08
  • Zeighami A, Dumas R, Aissaoui R. Knee loading in OA subjects is correlated to flexion and adduction moments and to contact point locations. Sci Rep. 2021;11(1):8594. doi:10.1038/s41598-021-87978-2
  • Miller RH, Krupenevich RL. Medial knee cartilage is unlikely to withstand a lifetime of running without positive adaptation: a theoretical biomechanical model of failure phenomena. PeerJ. 2020;8:e9676. doi:10.7717/peerj.9676
  • Bennell KL, Bowles K-A, Wang Y, et al. Higher dynamic medial knee load predicts greater cartilage loss over 12 months in medial knee osteoarthritis. Ann Rheum Dis. 2011;70(10):1770–1774. doi:10.1136/ard.2010.147082
  • Knowles N, Whittier DE, Besler BA, et al. Proximal tibia bone stiffness and strength in HR-pQCT- and QCT-based finite element models. Ann Biomed Eng. 2021;49(9):2389–2398. doi:10.1007/s10439-021-02789-w
  • Wang H, Wang R, Wang Z, et al. ClC-3 chloride channel functions as a mechanically sensitive channel in osteoblasts. Biochem Cell Biol. 2015;93(6):558–565. doi:10.1139/bcb-2015-0018
  • Harasztosi C, Gummer AW, Acsády L. The chloride-channel blocker 9-anthracenecarboxylic acid reduces the nonlinear capacitance of prestin-associated charge movement. Eur J Neurosci. 2016;43(8):1062–1074. doi:10.1111/ejn.13209
  • Li Z, Huang Z, Zhang H, et al. Moderate-intensity exercise alleviates pyroptosis by promoting autophagy in osteoarthritis via the P2X7/AMPK/mTOR axis. Cell Death Discov. 2021;7(1):346. doi:10.1038/s41420-021-00746-z