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Articles

Applicability of the MTT assay for measuring viability of cyanobacteria and algae, specifically for Microcystis aeruginosa (Chroococcales, Cyanobacteria)

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Pages 593-599 | Received 31 Jan 2007, Accepted 21 May 2007, Published online: 22 Apr 2019

REFERENCES

  • Agusti S. & Sanchez M.C. 2002. Cell viability in natural phytoplankton communities quantified by a membrane permeability probe. Limnology and Oceanography 47(3): 818–828.
  • Bernhard D., Schwaiger W., Crazzolara R., Tinhofer I., Kofler R. & Csordas A. 2003. Enhanced MTT-reducing activity under growth inhibition by resveratrol in CEM-C7H2 lymphocytic leukemia cells. Cancer Letters 195: 193–199.
  • Berridge M.V., Herst P.M. & Tan A.S. 2005. Tetrazolium dyes as tools in cell biology: new sights into their cellular reduction. Biotechnology Annual Review 11: 127–152.
  • Bidle K.D. & Falkowski P.G. 2004. Cell death in planktonic photosynthetic microorganisms. Nature Reviews Microbiology 2: 643–655.
  • Breeuwer P. & Abee T. 2000. Assessment of viability of microorganisms employing fluorescence techniques. International Journal of Food Microbiology 55: 193–200.
  • Brussaard C.P.D., Marie D., Thyrhaug R. & Bratbak G. 2001. Flow cytometric analysis of phytoplankton viability following viral infection. Aquatic Microbial Ecology 26: 157–166.
  • Capasso J.M., Cossio B.R., Berl T., Rivard C.J. & Jimenez C. 2003. A colorimetric assay for determination of cell viability in algal cultures. Biomolecular Engineering 20: 133–138.
  • Dorsey J., Yentsch C.M., Mayo S. & Mckenna C. 1989. Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry 10: 622–628.
  • Gaboriau F., Cheron M., Petit C. & Boland J. 1997. Heatinduced superaggregation of amphotericin B reduces its in vitro toxicity: a new way to improve its therapeutic index. Antimicrobial Agents and Chemotherapy 41(11): 2345–2351.
  • Jochem F.J. 1999. Dark survival strategies in marine phytoplankton assessed by cytometric measurement of metabolic activity with fluorescein diacetate. Marine Biology 135: 721–728.
  • Kairo S.K., Bedwell J., Tyler P.C., Carter A. & Corbel M.J. 1999. Development of a tetrazolium salt assay for rapid determination of viability of BCG vaccines. Vaccine 17: 2423–2428.
  • Keer J.T. & Birch L. 2003. Molecular methods for the assessment of bacterial viability. Journal of Microbiological Methods 53: 175–183.
  • Lee D.Y. & Rhee G.Y. 1997. Kinetics of cell death in the cyanobacterium Anabaena flos-aquae and the production of dissolved organic carbon. Journal of Phycology 33: 991–998.
  • Markelova A.G., Vladimirova M.G. & Kuptsova E.S. 2000. A comparison of cytochemical Methods for the rapid evaluation of microalgal viability. Russian Journal of Plant Physiology 47(6): 815–819.
  • Pouneva I. 1997. Evaluation of algal culture viability and physiological state by fluorescent microscopic methods. Bulgarian Journal of Plant Physiology 23(1–2): 67–76.
  • Sauer J., Schreiber U., Schmid R., Völker U. & Forchhammer K. 2001. Nitrogen starvation-induced chlorosis in Synechococcus PCC 7942: low level photosynthesis as a mechanism of long-term survival. Plant Physiology 126: 233–243.
  • Sieuwerts A.M., Klijn J.G., Peters H.A. & Foekens J.A. 1995. The MTT tetrazolium salt assay scrutinized: how to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, iC50-values and cell survival. European Journal of Clinical Chemistry and Clinical Biochemistry 33(11): 813–823.
  • Stentelaire C., Antoine N., Cabrol C., Feron G. & Durand A. 2001. Development of a rapid and highly sensitive biochemical method for the measurement of fungal spore viability: an alternative to the CFu method. Enzyme and Microbial Technology 29: 560–566.
  • Twentyman P.R. & Luscombe M. 1987. A study of some variables in a tetrazolium dye based assay for cell growth and chemosensitivity. British Journal of Cancer 56(3): 279–285.
  • Vardi A., Berman-Frank I., Rozenberg T., Hadas O., Kaplan A. & Levine A. 1999. Programmed cell death of dinoflagellate Peridinium gatunense is mediated by Co2 limitation and oxidative stress. Current Biology 9: 1061–1064.
  • Zhang Z.D. & Cox G. 1996. MTT assay overestimates human airway smooth muscle cell number in culture. Biochemistry & Molecular Biology International 38(3): 431–436.

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