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Articles

Morphology, phylogeny and toxin analysis of Pseudo-nitzschia pseudodelicatissima (Bacillariophyceae) isolated from the Thermaikos Gulf, Greece

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Pages 260-273 | Received 15 May 2009, Accepted 21 Oct 2009, Published online: 22 Apr 2019

REFERENCES

  • Adams N.G., Lesoing M. & Trainer V.L. 2000. Environmental conditions associated with domoic acid in razor clams on the Washington coast. Journal of Shellfish Research 19: 1007–1015.
  • Alamandox G.O., Ferreyra G.A., Schloss I.R., Dogliotti A.I., Rupolo V., Paparazzo F.E., Esteves J.L. & Ferrario M.E. 2008. Distribution and ecology of Pseudo-nitzschia species (Bacillariophyceae) in surface waters of the Weddell Sea (Antarctica). Polar Biology 31: 429–442.
  • Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25(17): 3389–3402.
  • Amato A. & Montresor M. 2008. Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudo-cryptic species within the P. pseudodelicatissima complex. Phycologia 47: 487–497.
  • Amato A., Kooistra W.H.C.F., Levialdi Ghiron J.H., Mann D.G., Pröschold T. & Montresor M. 2007. Reproductive isolation among sympatric cryptic species in marine diatoms. Protist 158: 193–207.
  • Andersen P. & Throndsen J. 2003. Estimating cell numbers. In: Manual on harmful marine microalgae (Ed. by G.M. Hallegraeff, D.M. Anderson & A.D. Cembella), pp. 99–129. Intergovernmental Oceanographic Commission (UNESCO), Paris.
  • Bargu S., Koray T. & Lundholm N. 2002. First report of Pseudo-nitzschia calliantha Lundholm, Moestrup & Hasle 2003, a new potentially toxic species from Turkish coasts. E.U. Journal of Fisheries and Aquatic Sciences 19: 479–483.
  • Bates S.S. 2000. Domoic-acid-producing diatoms: another genus added! Journal of Phycology 36: 978–983.
  • Bates S.S. & Trainer V.L. 2006. The ecology of harmful diatoms. In: Ecology of harmful algae (Ed. by E. Granéli & J. Turner), pp. 81–93. Springer-Verlag, Heidelberg.
  • Bates S.S., Bird C.J., De Freitas A.S.W., Foxall R., Gilgan M., Hanic L.A., Johnson G.R., McCulloch A.W., Odense P., Pocklington R., Quilliam M.A., Sim P.G., Smith J.C., Subba Rao D.V., Todd E.C.D., Walter J.A. & Wright J.L.C. 1989. Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada. Canadian Journal of Fisheries and Aquatic Sciences 46: 1203–1215.
  • Bates S.S., De Freitas A.S.W., Milley J.E., Pocklington R., Quilliam M.A., Smith J.C. & Worms J. 1991. Controls on domoic acid production by the diatom Nitzschia pungens f. multiseries in culture: nutrients and irradiance. Canadian Journal of Fisheries and Aquatic Sciences 48: 1136–1144.
  • Baugh K.A., Bush J.M., Bill B.D., Lefebvre K.A. & Trainer V.L. 2006. Estimates of specific toxicity in several Pseudo-nitzschia species from the Washington coast, based on culture and field studies. African Journal of Marine Sciences 28: 403–407.
  • Besiktepe S., Ryabushko L., Ediger D., Yilmaz D., Zenginer A., Ryabushko V. & Lee R. 2008. Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup & Hasle (Bacillariophyta) isolated from the Black Sea. Harmful Algae 7: 438–442.
  • Bill B.D., Lundholm N., Connell L., Baugh K.A. & Trainer V. 2005. Domoic acid in Pseudo-nitzschia cuspidata from Washington State coastal waters. 3rd Symposium on Harmful Algae in the US, Monterey, California, 77. [Abstract].
  • Caroppo C., Congestri R., Bracchini L. & Albertano P. 2005. On the presence of Pseudo-nitzschia calliantha Lundholm, Moestrup & Hasle and Pseudo-nitzschia delicatissima (Cleve) Heiden in the Southern Adriatic Sea (Mediterranean Sea, Italy). Journal of Plankton Research 27: 763–774.
  • Casteleyn G., Chepurnov V.A., Leliaert F., Mann D.G., Bates S.S., Lundholm N., Rhodes L., Sabbe K. & Vyverman W. 2008. Pseudo-nitzschia pungens (Bacillariophyceae): a cosmopolitan diatom species? Harmful Algae 7: 241–257.
  • Coleman A.W. 2000. The significance of a coincidence between evolutionary landmarks found in mating affinity and a DNA sequence. Protist 151: 1–9.
  • Cusack C.K., Bates S.S., Quilliam M.A., Patching J.W. & Raine R. 2002. Confirmation of domoic acid production by Pseudo-nitzschia australis (Bacillariophyceae) isolated from Irish waters. Journal of Phycology 38: 1106–1112.
  • D'alelio D., Amato A., Kooistra W.H.C.F., Procaccini G., Casotti R. & Montresor M. 2009. Internal transcribed spacer polymorphism in Pseudo-nitzschia multistriata (Bacillariophyceae) in the Gulf of Naples: recent divergence or intraspecific hybridization? Protist 160: 9–20.
  • Davidovich N.A. & Bates S.S. 1998. Sexual reproduction in the pennate diatoms Pseudo-nitzschia multiseries and P. pseudodelicatissima (Bacillariophyceae). Journal of Phycology 34: 126–137.
  • Douglas D.J. & Bates S.S. 1992. Production of domoic acid, a neurotoxic amino acid, by an axenic culture of the marine diatom Nitzschia pungens f. multiseries Hasle. Canadian Journal of Fisheries and Aquatic Sciences 49: 85–90.
  • Doyle J.J. & Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11–15.
  • Evans K.M., Bates S.S., Medlin L.K. & Hayes P.K. 2004. Microsatellite marker development and genetic variation in the toxic marine diatom Pseudo-nitzschia multiseries (Bacillariophyceae). Journal of Phycology 40: 911–920.
  • Fehling J., Davidson K., Bolch C.J. & Bates S.S. 2004a. Domoic acid production by Pseudo—nitzschia seriata (Bacillariophyceae) in Scottish waters. Journal of Phycology 40: 622–630.
  • Fehling J., Davisdon K., Bolch C.J. & Bates S.S. 2004b. Growth and domoic acid production by Pseudo-nitzschia seriata (Bacillariophyceae) under phosphate and silicate limitation. Journal of Phycology 40: 674–683.
  • Felsenstein J. 2005. PHYLIP (Phylogeny Inference Package) version 3.6. Department of Genome Sciences, University of Washington, Seattle. (Distributed by the author.)
  • Garrison D.L., Conrad S.M., Eilers P.P. & Waldron E.M. 1992. Confirmation of domoic acid production by Pseudo-nitzschia australis (Bacillariophyceae) cultures. Journal of Phycology 28: 604–607.
  • Guillard R.R.L. & Morton S.L. 2003. Culture methods. In: Manual on harmful marine microalgae (Ed. by G.M. Hallegraeff, D.M. Anderson & A.D. Cembella), pp. 77–97. Intergovernmental Oceanographic Commission (UNESCO), Paris.
  • Guindon S. & Gascuel O. 2003. A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology 52: 696–704.
  • Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  • Hallegraeff G.M. 1994. Species of the diatom genus Pseudo-nitzschia in Australian waters. Botanica Marina 37: 397–411.
  • Hasle G.R. 1965. Nitzschia and Fragilariopsis species studied in the light and electron microscopes. II. The group Pseudo-nitzschia. Skrifter Utgitt av Norske Videnskaps-Akademi I Oslo I Matematisk-Naturvideskapeling Klasse Ny Serie 18: 1–45.
  • Hasle G.R. 2002. Are most of the domoic acid producing species of the diatom genus Pseudo-nitzschia cosmopolites? Harmful Algae 1: 137–146.
  • Hasle G.R. & Fryxell G.A. 1970. Diatoms: cleaning and mounting for light and electron microscope. Transactions of the American Microsopical Society 89: 469–474.
  • Hasle G.R. & Syvertsen E.E. 1995. Marine diatoms. In: Identifying marine diatoms and dinoflagellates (Ed. by C.R. Tomas), pp. 5–385. Academic Press, San Diego.
  • Hasle G.R., Lange C.B. & Syvertsen E.E. 1996. A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters. Helgoländer Meeresuntersuchungen 50: 131–175.
  • Kaczmarska I., Legresley M.M., Martin J.L. & Ehrman J. 2005. Diversity of the diatom genus Pseudo-nitzschia Peragallo in the Quoddy Region of the Bay of Fundy, Canada. Harmful Algae 4: 1–19.
  • Kaniou-Grigoriadou I., Mouratidou T. & Katikou P. 2005. Investigation on the presence of domoic acid in Greek shellfish. Harmful Algae 4: 717–723.
  • Kimura M. 1981. Estimation of evolutionary distances between homologous nucleotide sequences. Proceedings of the National Academy of Sciences of the United States of America 78: 454–458.
  • Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thoompson J.D., Gibson T.J. & Higgins D.G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948.
  • Lenaers G., Maroteaux L., Michot B. & Herzog M. 1989. Dinoflagellates in evolution. A molecular phylogenetic analysis of large subunit ribosomal RNA. Journal of Molecular Evolution 29: 40–51.
  • Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., Jobb G., Förster W., Brettske I., Gerber S., Ginhart A.W., Gross O., Grumann S., Hermann S., Jost R., König A., Liss T., Lüssmann R., May M., Nonhoff B., Reichel B., Strehlow R., Stamatakis A., Stuckmann N., Vilbig A., Lenke M., Ludwig T., Bode A. & Schleifer K.H. 2004. ARB: a software environment for sequence data. Nucleic Acids Research 32: 1363–1371.
  • Lundholm N. & Moestrup Ø. 2002. The marine diatom Pseudonitzschia galaxiae sp. nov. (Bacillariophyceae): morphology and phylogenetic relationships. Phycologia 41: 594–605.
  • Lundholm N., Skov J., Pocklington R. & Moestrup O. 1994. Domoic acid, the toxic amino acid responsible for amnesic shellfish poisoning, now in Pseudonitzschia seriata (Bacillariophyceae) in Europe. Phycologia 33: 475–478.
  • Lundholm N., Skov J., Pocklington R. & Moestrup Ø. 1997. Studies on the marine planktonic diatom Pseudo-nitzschia. 2. Autecology of P. pseudodelicatissima based on isolates from Danish coastal waters. Phycologia 36: 381–388.
  • Lundholm N., Daugbjerg N. & Moestrup Ø. 2002. Phylogeny of the Bacillariaceae with emphasis on the genus Pseudo-nitzschia (Bacillariophyceae) based on partial LSU rDNA. European Journal of Phycology 37: 115–134.
  • Lundholm N., Moestrup Ø., Hasle G.R. & Hoef-Emden K. 2003. A study of the P. pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima? Journal of Phycology 39: 797–813.
  • Lundholm N., Moestrup Ø., Kotaki Y., Hoef-Emden K., Scholin C. & Miller P. 2006. Inter-and intraspecific variation of the Pseudo-nitzschia delicatissima complex (Bacillariophyceae) illustrated by rRNA probes, morphological data and phylogenetic analyses. Journal of Phycology 42: 464–481.
  • Mai J.C. & Coleman A.W. 1997. The internal transcribed spacer 2 exhibits a common secondary structure in green algae and flowering plants. Journal of Molecular Evolution 44: 258–271.
  • Martin J.L., Haya K., Burridge L.E. & Wildish D.J. 1990. Nitzschia pseudodelicatissima — a source of domoic acid in the Bay of Fundy, eastern Canada. Marine Ecology Progress Series 67: 177–182.
  • Mathews D.H., Disney M.D., Childs J.L., Schroeder S.J., Zuker M. & Turner D.H. 2004. Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure. Proceedings of the National Academy of Sciences of the United States of America 101: 7287–7292.
  • McDonald S.M., Sarno D. & Zingone A. 2007. Identifying Pseudo-nitzschia species in natural samples using genus-specific PCR primers and clone libraries. Harmful Algae 6: 849–860.
  • Moestrup Ø., Codd G.A., Elbrächter M., Faust M.A., Fraga S., Fukuyo Y., Cronberg G. & Halim Y. [Eds] (2009 onward). IOC-UNESCO taxonomic reference list of harmful microalgae. Available at: http://www.marinespecies.org/HAB. (14 September 2009).
  • Moschandreou K.K. & Nikolaidis G. 2010. The genus Pseudo-nitzschia (Bacillariophyceae) in Greek coastal waters. Botanica Marina 53: in press.
  • Müller T., Philippi N., Dandekar T., Schultz J. & Wolf M. 2007. Distinguishing species. RNA 13(9): 1469–1472.
  • Nikolaidis G., Koukaras K., Aligizaki K., Heracleous A., Kalopesa E., Moschandreou K., Tsolaki E. & Mantoudis A. 2005. Harmful microalgal episodes in Greek coastal waters. Journal of Biological Research Thessaloniki 3: 77–85.
  • Page R.D.M. 1996. TreeView: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12(4): 357–358.
  • Pan Y., Subba Rao D.V., Mann K.H., Brown R.G. & Pocklington R. 1996. Effect of silicate limitation on production of domoic acid, a neurotoxin, by the diatom Pseudo-nitzschia-multiseries. I. Batch culture studies. Marine Ecology Progress Series 131: 225–233.
  • Pan Y., Parsons M.L., Busman M., Moeller P.D.R., Dortch Q., Powell C.L. & Doucette G.J. 2001. Pseudo-nitzschia sp. cf. pseudodelicatissima — a confirmed producer of domoic acid from the northern Gulf of Mexico. Marine Ecology Progress Series 220: 83–92.
  • Parsons M.L., Scholin C.A., Miller P.E., Doucette G.J., Powell C.L., Fryxell G.A., Dortch Q. & Soniat T.M. 1999. Pseudo-nitzschia species (Bacillariophyceae) in Louisiana coastal waters: molecular probe field trials, genetic variability and domoic acid analyses. Journal of Phycology 35: 1368–1378.
  • Posada D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25(7): 1253–1256.
  • Priisholm K., Moestrup Ø. & Lundholm N. 2002. Taxonomic notes on the marine diatom genus Pseudo-nitzschia in the Andaman Sea, near the island of Phuket, Thailand, with a description of Pseudo-nitzschia micropora sp. nov. Diatom Research 17: 153–175.
  • Quijano-Scheggia S., Garces E., Sampedro N., Van Lenning K., Flo E., Andree K., Fortuño J.-M. & Camp J. 2008. Identification and characterisation of the dominant Pseudo-nitzschia species (Bacillariophyceae) along the NE Spanish coast (Catalonia, NW Mediterranean). Scientia Marina 72: 343–359.
  • Rhodes L., Scholin C. & Garthwaite I. 1998. Pseudo-nitzschia in New Zealand and the role of DNA probes and immunoassays in refining marine biotoxin monitoring programmes. Natural Toxins 6: 105–111.
  • Regulation 853/2004/EC. Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. Official Journal of the European Union L226: 22–82.
  • Ronquist F. & Huelsenbeck J.P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.
  • Sahraoui I., Sakka Hlaili A., Hadj Mabrouk H., Léger C. & Bates S.S. 2009. Blooms of the genus Pseudo-nitzschia H. Peragallo in Bizerte Lagoon (Tunisia, SW Mediterranean). Diatom Research 24: 175–190.
  • Schloss P.D. & Handelsman J. 2005. Introducing DOTUR, a computer program for defining operational taxonomic units and species richness. Applied and Environmental Microbiology 71: 1501–1506.
  • Scholin C.A., Villac M.C., Buck K.R., Krupp J.M., Powers D.A., Fryxell G.A. & Chavez F.P. 1994. Ribosomal DNA sequences discriminate among toxic and non-toxic Pseudo-nitzschia species. Natural Toxins 2: 152–165.
  • Schultz J., Maisel S., Gerlach D., Müller T. & Wolf M. 2005. A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. RNA 11: 361–364.
  • Siebert S. & Backofen R. 2005. MARNA: multiple alignment and consensus structure prediction of RNAs based on sequence structure comparisons. Bioinformatics 21(16): 3352–3359.
  • Sierra-Beltrán A.P., Cortés-Altamirano R., Gallo-Reynoso J.P., Licea-Duran S. & Égido-Villarreal, J. 2005. Is Pseudo-nitzschia pseudodelicatissima toxin the principal cause of sardines, dolphins, sea lions and pelicans mortality in 2004 in Mexico? Harmful Algae News 29: 6–8.
  • Spatharis S., Danielidis D.B. & Tsirtsis G. 2007. Recurrent Pseudo-nitzschia calliantha (Bacillariophyceae) and Alexandrium insuetum (Dinophyceae) winter blooms induced by agricultural runoff. Harmful Algae 6: 811–822.
  • Stehr C.M., Connell L., Baugh K.A., Bill B.D., Adams N.G. & Trainer V.L. 2002. Morphological, toxicological, and genetic differences among Pseudo-nitzschia (Bacillariophyceae) species in inland embayments and outer coastal waters of Washington State, USA. Journal of Phycology 38: 55–65.
  • Subba Rao D.V., Quilliam M.A. & Pocklington R. 1988. Domoic acid — a neurotoxic amino acid produced by the marine diatom Nitzschia pungens in culture. Canadian Journal of Fisheries and Aquatic Sciences 45: 2076–2079.
  • Trainer V.L., Cochlan W.P., Erickson A., Bill B.D., Cox F.H., Borchert J.A. & Lefebvre K.A. 2007. Recent domoic acid closures of shellfish harvest areas in Washington State inland waterways. Harmful Algae 6: 449–459.
  • Utermöhl H. 1958. Zur vervollkommung der quantitativen Phytoplankton Methodik. Mitteilungen der internationale Vereinigung für theoretische und angewandte Limnologie 9: 1–38.
  • Van Nues R.W., Rientjes J.M.J., Servaas A.M., Mollee E., Planta R.J., Venema J. & Raué H.A. 1995a. Evolutionarily conserved structural elements are critical for processing of internal transcribed spacer 2 from Saccharomyces cerevisiae precursor ribosomal RNA. Journal of Molecular Biology 250(1): 24–36.
  • Van Nues R.W., Venema J., Rientjes J.M., Dirks-Mulder A. & Raué H.A. 1995b. Processing of eukaryotic pre-rRNA: the role of the transcribed spacers. Biochemistry and Cell Biology 73: 789–801.
  • White T.J., Bruns T., Lee S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications (Ed. by M.A. Innis, D.H. Gelfand, J.J. Sninsky & T.J. White), pp. 315–322. Academic Press, London.
  • Wolf M., Friedrich J., Dandekar T. & Müller T. 2005. CBCAnalyzer: inferring phylogenies based on compensatory base changes in RNA secondary structures. In Silico Biology 5(3): 291–294.

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