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Research Article

Epitranscriptomic Analysis of m6A Methylome in Rats after Lumbosacral Nerve Root Avulsion

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Pages 589-600 | Received 16 Apr 2022, Accepted 28 Apr 2022, Published online: 16 May 2022

References

  • Sidhu JS , DhillonMK. Lumbosacral plexus avulsion with pelvic fractures. Injury22(2), 156–158 (1991).
  • Chin CH , ChewKC. Lumbosacral nerve root avulsion. Injury28(9–10), 674–678 (1997).
  • Gu HY , ChaiH , ZhangJYet al. Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion. Eur. J. Neurosci.19(8), 2123–2131 (2004).
  • Zhu L , ZhangF , YangD , ChenA. The effect of severing a normal S1 nerve root to use for reconstruction of an avulsed contralateral lumbosacral plexus: a pilot study. Bone Joint J.97-B(3), 358–365 (2015).
  • Liu J , YueY , HanDet al. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat. Chem. Biol.10(2), 93–95 (2014).
  • Schumann U , ShafikA , PreissT. METTL3 gains R/W access to the epitranscriptome. Mol. Cell62(3), 323–324 (2016).
  • Ping XL , SunBF , WangLet al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Res.24(2), 177–189 (2014).
  • Jia G , FuY , ZhaoXet al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO. Nat. Chem. Biol.7(12), 885–887 (2011).
  • Zheng G , DahlJA , NiuYet al. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Mol. Cell49(1), 18–29 (2013).
  • Wang X , LuZ , GomezAet al. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature505(7481), 117–120 (2014).
  • Dominissini D , Moshitch-MoshkovitzS , SchwartzSet al. Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature485(7397), 201–206 (2012).
  • Wang X , ZhaoBS , RoundtreeIAet al. N(6)-methyladenosine modulates messenger RNA translation efficiency. Cell161(6), 1388–1399 (2015).
  • Du K , ZhangZ , ZengZ , TangJ , LeeT , SunT. Distinct roles of Fto and Mettl3 in controlling development of the cerebral cortex through transcriptional and translational regulations. Cell Death Dis.12(7), 700 (2021).
  • Ma C , ChangM , LvHet al. RNA m(6)A methylation participates in regulation of postnatal development of the mouse cerebellum. Genome Biol.19(1), 68 (2018).
  • Yu J , ZhangY , MaHet al. Epitranscriptomic profiling of N6-methyladenosine-related RNA methylation in rat cerebral cortex following traumatic brain injury. Mol. Brain13(1), 11 (2020).
  • Zhang L , HaoD , MaPet al. Epitranscriptomic analysis of m6A methylome after peripheral nerve injury. Front. Genet.12, 686000 (2021).
  • Zhu L , HuangG , ShengJ , FuQ , ChenA. High-mobility group box 1 induces neuron autophagy in a rat spinal root avulsion model. Neuroscience315, 286–295 (2016).
  • Dominissini D , Moshitch-MoshkovitzS , Salmon-DivonM , AmariglioN , RechaviG. Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing. Nat. Protoc.8(1), 176–189 (2013).
  • Bolger AM , LohseM , UsadelB. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics30(15), 2114–2120 (2014).
  • Kim D , LangmeadB , SalzbergSL. HISAT: a fast spliced aligner with low memory requirements. Nat. Methods12(4), 357–360 (2015).
  • Cui X , ZhangL , MengJ , RaoMK , ChenY , HuangY. MeTDiff: a novel differential RNA methylation analysis for MeRIP-seq data. IEEE/ACM Trans. Comput. Biol. Bioinform.15(2), 526–534 (2018).
  • Yu G , WangLG , HeQY. ChIPseeker: an R/Bioconductor package for ChIP peak annotation, comparison and visualization. Bioinformatics31(14), 2382–2383 (2015).
  • Boccaletto P , MachnickaMA , PurtaEet al. MODOMICS: a database of RNA modification pathways. 2017 update. Nucleic Acids Res.46(D1), D303–D307 (2018).
  • Church C , MoirL , McMurrayFet al. Overexpression of Fto leads to increased food intake and results in obesity. Nat. Genet.42(12), 1086–1092 (2010).
  • Xiao S , ZengX , FanYet al. Gene polymorphism association with type 2 diabetes and related gene–gene and gene–environment interactions in a Uyghur population. Med. Sci. Monit.22, 474–487 (2016).
  • Wu Y , XieL , WangMet al. Mettl3-mediated m(6)A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis. Nat. Commun.9(1), 4772 (2018).
  • Chen XY , ZhangJ , ZhuJS. The role of m(6)A RNA methylation in human cancer. Mol. Cancer18(1), 103 (2019).
  • Wang H , HuX , HuangMet al. Mettl3-mediated mRNA m(6)A methylation promotes dendritic cell activation. Nat. Commun.10(1), 1898 (2019).
  • Wang Y , MaoJ , WangXet al. Genome-wide screening of altered m6A-tagged transcript profiles in the hippocampus after traumatic brain injury in mice. Epigenomics11(7), 805–819 (2019).
  • Xing L , CaiY , YangTet al. Epitranscriptomic m6A regulation following spinal cord injury. J. Neurosci. Res.99(3), 843–857 (2021).
  • Li Y , GuoX , SunLet al. N(6)-Methyladenosine demethylase FTO contributes to neuropathic pain by stabilizing G9a expression in primary sensory neurons. Adv. Sci. (Weinh.)7(13), 1902402 (2020).
  • Furlan JC , LiuY , DietrichWD3rd , NorenbergMD , FehlingsMG. Age as a determinant of inflammatory response and survival of glia and axons after human traumatic spinal cord injury. Exp. Neurol.332, 113401 (2020).
  • Tian R , WuB , FuC , GuoK. miR-137 prevents inflammatory response, oxidative stress, neuronal injury and cognitive impairment via blockade of Src-mediated MAPK signaling pathway in ischemic stroke. Aging (Albany NY)12(11), 10873–10895 (2020).
  • Li N , LimG , ChenLet al. Spinal expression of Hippo signaling components YAP and TAZ following peripheral nerve injury in rats. Brain Res.1535, 137–147 (2013).
  • Ponnusamy V , IpR , MohamedMet al. Neuronal let-7b-5p acts through the Hippo-YAP pathway in neonatal encephalopathy. Commun. Biol.4(1), 1143 (2021).
  • Pu J , WangJ , QinZet al. IGF2BP2 promotes liver cancer growth through an m6A-FEN1-dependent mechanism. Front. Oncol.10, 578816 (2020).

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