Advanced search
Publication Cover
Nanomedicine Volume 12, 2017 - Issue 10
Submit an article Journal homepage
10,915
Views
0
CrossRef citations to date
0
Altmetric
Commentary

Quantifying Nanoparticle Cellular Uptake: Which Method is Best?

Barbara Drasler1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg1700, Switzerland
,
Dimitri Vanhecke1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg1700, Switzerland
,
Laura Rodriguez-Lorenzo1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg1700, Switzerland
,
Alke Petri-Fink1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg1700, Switzerland;2 Department of Chemistry, University of Fribourg, Chemin du Museé 9, Fribourg1700, Switzerland
&
Barbara Rothen-Rutishauser1 Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, Fribourg1700, SwitzerlandCorrespondence[email protected]
Pages 1095-1099 | Received 28 Feb 2017, Accepted 09 Mar 2017, Published online: 27 Apr 2017

References

  • Elsaesser A Taylor A De Yanes GS et al. Quantification of nanoparticle uptake by cells using microscopical and analytical techniques. Nanomedicine5 (9), 1447 – 1457 (2010).
  • Vanhecke D Rodriguez-Lorenzo L Clift MJD Blank F Petri-Fink A Rothen-Rutishauser B . Quantification of nanoparticles at the single-cell level: an overview about state-of-the-art techniques and their limitations. Nanomedicine9 (12), 1885 – 1900 (2014).
  • Collins AR Annangi B Rubio L et al. High throughput toxicity screening and intracellular detection of nanomaterials. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol.9 (1), doi:10.1002/wnan.1413 (2017) ( Epub ahead of print).
  • Rashkow JT Patel SC Tappero R Sitharaman B . Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population. J. R. Soc. Interface11 (94), doi:10.1098/rsif.2013.1152 (2014) (Epub ahead of print.)
  • Williams JH . The tyrany of numbers. In : Quantifying Measurement.Morgan & Claypool Publishers, CA, USA, 4433 – 4439 (2016).
  • Wilks SS . Some aspects of quantification in science. Isis52 (168), 135 – 142 (1961).
  • Tiwari G Tiwari R . Bioanalytical method validation: an updated review. Pharm. Meth.1 (1), 25 – 38 (2010).
  • Kim JA Aberg C Salvati A Dawson KA . Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population. Nat. Nano.7 (1), 62 – 68 (2012).
  • Jeynes JCG Jeynes C Merchant MJ Kirkby KJ . Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles. Analyst138 (23), 7070 – 7074 (2013).
  • Montaser A Golightly DW . Inductively Induced Coupled Plasmas in Analytic Atomic Spectra (2nd Edition).Wiley-Blackwell, NJ, USA, 1040 (1992).
  • Taylor H . Inductively Coupled Plasma-Mass Spectrometry.Academic Press, MA, USA, 294 (2000).
  • Krystek P Ulrich A Garcia CC Manohar S Ritsema R . Application of plasma spectrometry for the analysis of engineered nanoparticles in suspensions and products. J. Anal. Atomic Spectrometry26 (9), 1701 – 1721 (2011).
  • Gottstein C Wu GH Wong BJ Zasadzinski JA . Precise quantification of nanoparticle internalization. Acs. Nano.7 (6), 4933 – 4945 (2013).
  • Montano MD Olesik JW Barber AG Challis K Ranville JF . Single particle ICP-MS: advances toward routine analysis of nanomaterials. Anal. Bioanalyt. Chem.408 (19), 5053 – 5074 (2016).
  • Chen M He XX Wang KM He DG Yang XH Shi H . Inorganic fluorescent nanoprobes for cellular and subcellular imaging. Trac.-Trend. Anal. Chem.58120 – 129 (2014).
  • Zucker RM Massaro EJ Sanders KM Degn LL Boyes WK . Detection of TiO2 nanoparticles in cells by flow cytometry. Cytometry A77 (7), 677 – 685 (2010).
  • Shapero K Fenaroli F Lynch I Cottell DC Salvati A Dawson KA . Time and space resolved uptake study of silica nanoparticles by human cells. Mol. BioSystems7 (2), 371 – 378 (2011).
  • Waters JC . Accuracy and precision in quantitative fluorescence microscopy. J. Cell Biol.185 (7), 1135 – 1148 (2009).
  • Sterio DC . The unbiased estimation of number and sizes of arbitrary particles using the disector. J. Microsc.134 (Pt 2), 127 – 136 (1984).
  • Vanhecke D Studer D Ochs M . Stereology meets electron tomography: towards quantitative 3D electron microscopy. J. Struct. Biol.159 (3), 443 – 450 (2007).
  • Brandenberger C Ochs M Muhlfeld C . Assessing particle and fiber toxicology in the respiratory system: the stereology toolbox. Part. Fibre Toxicol.12, 1 – 15 (2015).
  • Gundersen HJG Jensen EB . The efficiency of systematic-sampling in stereology and its prediction. J. Microsc.147229 – 263 (1987).

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.