372
Views
23
CrossRef citations to date
0
Altmetric
Commentary

Quantitative analysis of fluorescence lifetime imaging made easy

&
Pages 7-11 | Received 17 Dec 2007, Published online: 07 Sep 2010

References

  • Caiolfa , VR , Zamai , M , Malengo , G , Andolfo , A , Madsen , CD , Sutin , J , Digman , MA , Gratton , E , Blasi , F and Sidenius , N . 2007 . “Monomer dimer dynamics and distribution of GPI‐anchored uPAR are determined by cell surface protein assemblies.” . J. Cell Biol. , 179 : 1067 – 1082 .
  • Clayton , AH , Hanley , QS and Verveer , PJ . 2004 . “Graphical representation and multicomponent analysis of single‐frequency fluorescence lifetime imaging microscopy data.” . J. Microsc. , 213 : 1 – 5 .
  • Colyer , RA , Lee , C and Gratton , E . 2007 . “A novel fluorescence lifetime imaging system that optimizes photon efficiency.” . Microsc. Res. Tech. ,
  • Digman , M , Caiolfa , VR , Zamai , M and Gratton , E . 2007 . “The Phasor approach to fluorescence lifetime imaging analysis.” . Biophys. J. , 94 : L14 – 6 .
  • Esposito , A , Gerritsen , HC , Lustenberger , F , Oggier , T and Wouters , FS . 2006 . “Innovating lifetime microscopy: a compact and simple tool for the life sciences, screening and diagnostics.” . J. Biomed. Opt. , 11 : 34016
  • Esposito , A , Gerritsen , HC and Wouters , FS . 2005 . “Fluorescence lifetime heterogeneity resolution in the frequency‐domain by Lifetime Moments Analysis (LiMA).” . Biophys. J. , 89 : 4286 – 4299 .
  • Esposito , A , Gerritsen , HC and Wouters , FS . 2007 . “Optimizing frequency‐domain fluorescence lifetime sensing for high‐throughput applications: photon‐economy and acquisition speed.” . J. Opt. Soc. Am. A , 10 : 3261 – 3273 .
  • Forde , TS and Hanley , QS . 2006 . “Spectrally resolved frequency domain analysis of multi‐fluorophore systems undergoing energy transfer.” . Appl. Spectrosc. , 60 : 1442 – 1452 .
  • Hanley , QS and Clayton , AH . 2005 . “AB‐plot assisted determination of fluorophore mixtures in a fluorescence lifetime microscope using spectra or quenchers.” . J. Microsc. , 218 : 62 – 67 .
  • Jameson , DM , Gratton , E and Hall , RD . 1984 . “The measurement and analysis of heterogeneous emissions by multifrequency phase and modulation fluorometry.” . Appl. Spectrosc. Rev. , 20 : 55 – 106 .
  • Lakowicz , JR and Balter , A . 1982 . “Theory of phase‐modulation fluorescence spectroscopy for excited‐state processes.” . Biophys. Chem. , 16 : 99 – 115 .
  • Pelet , S , Previte , MJ , Laiho , LH and So , PT . 2004 . “A fast global fitting algorithm for fluorescence lifetime imaging microscopy based on image segmentation.” . Biophys. J. , 87 : 2807 – 2817 .
  • Redford , GI and Clegg , RM . 2005 . “Polar plot representation for frequency‐domain analysis of fluorescence lifetimes.” . J. Fluoresc. , 15 : 805 – 815 .
  • Verveer , PJ and Bastiaens , PI . 2003 . “Evaluation of global analysis algorithms for single frequency fluorescence lifetime imaging microscopy data.” . J. Microsc. , 209 : 1 – 7 .
  • Wouters , FS , Verveer , PJ and Bastiaens , PI . 2001 . “Imaging biochemistry inside cells.” . Trends Cell Biol. , 11 : 203 – 211 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.