In vitro antimicrobial and anti-endotoxin action of Zingiber Officinale as auxiliary chemical and medicament combined to calcium hydroxide and chlorhexidine

References

• Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965;20:340–9.
   PubMedGoogle Scholar
• Nair PN, Sjogren U, Krey G, Kahnberg KE, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 1990;16:580–8.
   PubMed Web of Science ®Google Scholar
• Waltimo TM, Siren EK, Torkko HL, Olsen I, Haapasalo MP. Fungi in therapy-resistant apical periodontitis. Int Endod J 1997;30:96–101.
   PubMed Web of Science ®Google Scholar
• Siqueira JFJr, Rocas IN, Lopes HP. Patterns of microbial colonization in primary root canal infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:174–8.
   Web of Science ®Google Scholar
• Sunde PT, Olsen I, Debelian GJ, Tronstad L. Microbiota of periapical lesions refractory to endodontic therapy. J Endod 2002;28:304–10.
   PubMedGoogle Scholar
• Jacinto RC, Gomes BP, Shah HN, Ferraz CC, Zaia AA, Souza-Filho FJ. Quantification of endotoxins in necrotic root canals from symptomatic and asymptomatic teeth. J Med Microbiol 2005;54:777–83.
   Google Scholar
• Martinho FC, Gomes BP. Quantification of endotoxins and cultivable bacteria in root canal infection before and after chemomechanical preparation with 2.5% sodium hypochlorite. J Endod 2008;34:268–72.
   Google Scholar
• Oliveira LD, Jorge AO, Carvalho CA, Koga-Ito CY, Valera MC. In vitro effects of endodontic irrigants on endotoxins in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;104:135–42.
   Google Scholar
• Valera MC, da Rosa JA, Maekawa LE, de Oliveira LD, Carvalho CA, Koga-Ito CY, et al. Action of propolis and medications against Escherichia coli and endotoxin in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e70–4.
   Google Scholar
• Siqueira JFJr, Rocas IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97:85–94.
   PubMed Web of Science ®Google Scholar
• Gomes BP, Pinheiro ET, Jacinto RC, Zaia AA, Ferraz CC, Souza-Filho FJ. Microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction. J Endod 2008;34:537–40.
   PubMed Web of Science ®Google Scholar
• Gomes BP, Vianna ME, Sena NT, Zaia AA, Ferraz CC, de Souza Filho FJ. In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:544–50.
   Google Scholar
• Lantz RC, Chen GJ, Sarihan M, Solyom AM, Jolad SD, Timmermann BN. The effect of extracts from ginger rhizome on inflammatory mediator production. Phytomedicine 2007;14:123–8.
   PubMed Web of Science ®Google Scholar
• Park M, Bae J, Lee DS. Antibacterial activity of [10]-gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res 2008;22:1446–9.
   PubMed Web of Science ®Google Scholar
• Csako G, Elin RJ, Hochstein HD, Tsai CM. Physical and biological properties of U.S. standard endotoxin EC after exposure to ionizing radiation. Infect Immun 1983;41:190–6.
   Google Scholar
• Valera MC, Silva KC, Maekawa LE, Carvalho CA, Koga-Ito CY, Camargo CH, et al. Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals. J Appl Oral Sci 2009;17:555–9.
   Google Scholar
• Valera MC, Maekawa LE, de Oliveira LD, Jorge AO, Shygei E, Carvalho CA. In vitro antimicrobial activity of auxiary chemical substances and natural extracts on Candida albicans and Enterococcus faecalis in root canals. J Appl Oral Sci 2013;21:118–23.
   PubMed Web of Science ®Google Scholar
• Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, Jorge AO. Effect of Zingiber Officinale and Propolis on microorganisms and endotoxins in root canals. J Appl Oral Sci 2013;21:25–31.
   Google Scholar
• Valera MC, Maekawa LE, Chung A, Cardoso FGR, Oliveira LD, Oliveira CL, et al. The effect of sodium hypochlorite and ginger extract on microrganisms and endotoxins in endodontic treatment of infected root canals. Gen Dent 2014;62:25–9.
   Google Scholar
• Waltimo TM, Siren EK, Orstavik D, Haapasalo MP. Susceptibility of oral Candida species to calcium hydroxide in vitro. Int Endod J 1999;32:94–8.
   PubMed Web of Science ®Google Scholar
• Gondim JO, Avaca-Crusca JS, Valentini SR, Zanelli CF, Spolidorio DM, Giro EM. Effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against Porphyromonas gingivalis and Enterococcus faecalis. Int J Paediatr Dent 2012;22:116–24.
   Google Scholar
• Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002;35:221–8.
   PubMed Web of Science ®Google Scholar
• Martins AP, Salgueiro L, Goncalves MJ, da Cunha AP, Vila R, Canigueral S, et al. Essential oil composition and antimicrobial activity of three Zingiberaceae from S.Tome e Principe. Planta Med 2001;67:580–4.
   PubMed Web of Science ®Google Scholar
• Mascolo N, Jain R, Jain SC, Capasso F. Ethnopharmacologic investigation of ginger (Zingiber officinale). J Ethnopharmacol 1989;27:129–40.
   PubMed Web of Science ®Google Scholar
• Alzorekya N, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol 2003;80:7.
   Google Scholar
• Habsah M, Amran M, Mackeen MM, Lajis NH, Kikuzaki H, Nakatani N, et al. Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. J Ethnopharmacol 2000;72:403–10.
   PubMed Web of Science ®Google Scholar
• Vianna ME, Horz HP, Conrads G, Zaia AA, Souza-Filho FJ, Gomes BP. Effect of root canal procedures on endotoxins and endodontic pathogens. Oral Microbiol Immunol 2007;22:411–18.
   Google Scholar
• Kim M, Hamilton SE, Guddat LW, Overall CM. Plant collagenase: unique collagenolytic activity of cysteine proteases from ginger. Biochim Biophys Acta 2007;1770:1627–35.
   PubMed Web of Science ®Google Scholar
• Fouda AM, Berika MY. Evaluation of the effect of hydroalcoholic extract of Zingiber officinale rhizomes in rat collagen-induced arthritis. Basic Clin Pharmacol Toxicol 2009;104:262–71.
   PubMed Web of Science ®Google Scholar
• Silveira CF, Cunha RS, Fontana CE, de Martin AS, Gomes BP, Motta RH, et al. Assessment of the antibacterial activity of calcium hydroxide combined with chlorhexidine paste and other intracanal medications against bacterial pathogens. Eur J Dent 2011;5:1–7.
   Google Scholar
• Gomes BP, Montagner F, Berber VB, Zaia AA, Ferraz CC, de Almeida JF, et al. Antimicrobial action of intracanal medicaments on the external root surface. J Dent 2009;37:76–81.
   Google Scholar
• Siqueira JFJr, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J 1999;32:361–9.
   PubMed Web of Science ®Google Scholar