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NOSE / SINUS

Isolation and culture of primary human nasal epithelial cells from anesthetized nasal epithelia

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Pages 296-299 | Received 30 Aug 2013, Accepted 17 Oct 2013, Published online: 23 Dec 2013

References

  • Jiao J, Wang H, Meng N, Zhang L. Different cilia response to adenosine triphosphate or benzalkonium chloride treatment in mouse nasal and tracheal culture. ORL J Otorhinolaryngol Relat Spec 2012;74:280–5.
  • Bleier BS, Mulligan RM, Schlosser RJ. Primary human sinonasal epithelial cell culture model for topical drug delivery in patients with chronic rhinosinusitis with nasal polyposis. J Pharm Pharmacol 2012;64:449–56.
  • Mallants R, Vlaeminck V, Jorissen M, Augustijns P. An improved primary human nasal cell culture for the simultaneous determination of transepithelial transport and ciliary beat frequency. J Pharm Pharmacol 2009;61:883–90.
  • Fulcher ML, Randell SH. Human nasal and tracheo-bronchial respiratory epithelial cell culture. Methods Mol Biol 2013;945:109–21.
  • Pifferi M, Montemurro F, Cangiotti AM, Ragazzo V, Di Cicco M, Vinci B, et al. Simplified cell culture method for the diagnosis of atypical primary ciliary dyskinesia. Thorax 2009;64:1077–81.
  • Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T. The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis. Clin Exp Allergy 2012;42:218–28.
  • Servetnyk Z, Krjukova J, Gaston B, Zaman K, Hjelte L, Roomans GM, et al. Activation of chloride transport in CF airway epithelial cell lines and primary CF nasal epithelial cells by S-nitrosoglutathione. Respir Res 2006;7:124.
  • Smith MW, Morton AW, Jones RT, Kahng MW, Resau JH. Methods for improving tissue culture of human tracheo-bronchial epithelium obtained at autopsy. Cytotechnology 1991;5:155–63.
  • Lane C, Burgess S, Kicic A, Knight D, Stick S. The use of non-bronchoscopic brushings to study the paediatric airway. Respir Res 2005;6:53.
  • Bridges MA, Walker DC, Davidson AG. Cystic fibrosis and control nasal epithelial cells harvested by a brushing procedure. In Vitro Cell Dev Biol 1991;27A:684–6.
  • Harris CM, Mendes F, Dragomir A, Doull IJ, Carvalho-Oliveira I, Bebok Z, et al. Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing. J Cyst Fibros 2004;3:43–8.
  • de Courcey F, Zholos AV, Atherton-Watson H, Williams MT, Canning P, Danahay HL, et al. Development of primary human nasal epithelial cell cultures for the study of cystic fibrosis pathophysiology. Am J Physiol Cell Physiol 2012;303:C1173–9.
  • Mosler K, Coraux C, Fragaki K, Zahm JM, Bajolet O, Bessaci-Kabouya K, et al. Feasibility of nasal epithelial brushing for the study of airway epithelial functions in CF infants. J Cyst Fibros 2008;7:44–53.
  • Olin JT, Burns K, Carson JL, Metjian H, Atkinson JJ, Davis SD, et al. Diagnostic yield of nasal scrape biopsies in primary ciliary dyskinesia: a multicenter experience. Pediatr Pulmonol 2011;46:483–8.
  • McDougall CM, Blaylock MG, Douglas JG, Brooker RJ, Helms PJ, Walsh GM. Nasal epithelial cells as surrogates for bronchial epithelial cells in airway inflammation studies. Am J Respir Cell Mol Biol 2008;39:560–8.
  • Romagnoli M, Vachier I, Vignola AM, Godard P, Bousquet J, Chanez P. Safety and cellular assessment of bronchial brushing in airway diseases. Respir Med 1999;93:461–6.
  • Rutland J, Griffin W, Cole PJ. An in vitro model for studying the effects of pharmacological agents on human ciliary beat frequency: effects of lignocaine. Br J Clin Pharmacol 1982;13:679–83.
  • Toskala E, Haataja J, Shirasaki H, Rautiainen M. Culture of cells harvested with nasal brushing: a method for evaluating ciliary function. Rhinology 2005;43:121–4.
  • Comer DM, Elborn JS, Ennis M. Comparison of nasal and bronchial epithelial cells obtained from patients with COPD. PLoS One 2012;17:e3292.

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