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Original Article

Cell Separation: A Review

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Pages 53-62 | Published online: 20 Aug 2009

References

  • Shortman K. Physical procedures for the separation of animal cells. Ann Rev Biophys Bioeng 1972; 1: 93–130
  • Pretlow T G, Weir E E, Zettergren J G. Problems connected with the separation of different kinds of cells. Int Rev Exp Pathol 1975; 14: 91–204
  • Harwood R. Cell separation by gradient centrifugation. Int Rev Cytol 1974; 38: 369–403
  • Shortman K. The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation. Aust J Exp Biol Med Sci 1968; 46: 375–96
  • Gorczynski R M, Miller R G, Phillips R A. Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll. Immunology 1970; 19: 817–29
  • Pertoft H, Laurent T C. Isopycnic separation of cells and organelles by centrifugation in modified silica gradients. Methods of cell separation, Vol 1, N Catsimpoolas. Plenum, New York 1977; 25–65
  • Munthe-Kaas A C, Seglen P O. The use of metrizamide as a gradient medium for isopycnic separation of rat liver cells. FEBS Lett 1974; 43: 252–6
  • Ford T C, Rickwood D. Formation of isotonic Nycodenz gradients for cell separations. Anal Biochem 1982; 124: 293–8
  • Tulp A, Kooi M W, Kipp J BA, et al. A separation chamber to sort cells, nuclei and chromosomes at moderate g forces. II. Studies on velocity sedimentation and equilibrium density gradient centrifugation of mammalian cells. Anal Biochem 1981; 117: 354–65
  • De Duve C. Tissue fractionation: past and present. J Cell Biol 1971; 50: 20d–55d
  • Shortman K. Separation methods for lymphocyte populations. Contemp Top Mol Immunol 1974; 3: 161–203
  • Currie G A, Hedley D W, Nyholm R E, Taylor S A. Contamination of mononuclear cell suspensions obtained from cancer patients b the Bdyum method. Br J Cancer 1978; 38: 555–6
  • Kumar R K, Lykke A WJ. Granulocyte contamination of separated blood mononuclear cells from spontaneously tumorous mice. Br J Cancer 1980; 42: 794–6
  • Gmelig-Meyling F, Waldmann T A. Separation of human blood monocytes and lymphocytes on a continuous Percoll gradient. J Immunol Methods 1980; 33: 1–9
  • Penttila l A, Keefe D E, Jenkin C R. A single-step method for the enrichment of murine peripheral blood eosinophils. J Immunol Methods 1982; 51: 119–23
  • Raghuprasad P K. A rapid simple method of basophil purification by density centrifugation on Percoll. J Immunol 1982; 129: 2128–33
  • Davies M DJ, Parrott D MV. Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut 1981; 22: 481–8
  • Enerbäck L, Svensson I. Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll. J Immunol Methods 1980; 39: 135–45
  • Dalet C, Fehlmann M, Debey P. Use of Percoll density gradient centrifugation for preparing isolated rat hepatocytes having long-term viability. Anal Biochem 1982; 122: 119–23
  • Knook D L, Blansjaar N, Sleyster E C. Isolation and characterisation of Kupffer and endothelial cells from the rat liver. Exp Cell Res 1977; 109: 317–29
  • Patnaik R, Mazumder A, Bhagavan B S, Nair P P. Characterisation of rat colonic epithelial cell populations. Cell Differ 1981; 10: 147–56
  • Vinay P, Gougoux A, Lemieux G. Isolation of a pure suspension of rat proximal tubules. Am J Physiol 1981; 241: F403–11
  • Brysk M M, Snider J M, Smith E B. Separation of newborn rat epidermal cells on discontinuous isokinetic gradients of Percoll. J Invest Dermatol 1981; 77: 205–9
  • Gale J S, Wakefield J SJ, Ford H C. Isolation of rat Leydig cells by density gradient centrifugation. J Endocrinol 1982; 92: 293–302
  • Huttner W B, Meyermann R, Neuhoff V, Althaus H H. Neurochemical and morphological studies of bulk isolated rat brain cells. II. Preparation of viable cerebral neurons which retain synaptic complexes. Brain Res 1979; 171: 225–37
  • Haley N J, Shio H, Fow ler S. Characterisation of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation. Lab Invest 1977; 37: 287–96
  • Sigdestad C P, Grdina D J. Density centrifugation of murine fibrosarcoma cells following in situ labelling with tritiated thymidine. Cell Tissue Kinet 1981; 14: 589–600
  • Bosslet K, Ruffman R, Altevogt P, Schirrmacher V. A rapid method for the isolation of metastasising tumour cells from internal organs with the help of isopycnic density gradient centrifugation in Percoll. Br J Cancer 1981; 44: 356–62
  • Kramer K J, Kan S C, Siddiqui W A. Concentration of Plasmodium falciparum-infected erythrocytes by density gradient centrifugation in Percoll. J Parasitol 1982; 68: 336–7
  • Miller R G. Separation of cells by velocity sedimentation. New techniques in biophysics and cell biology, Vol. 1, R H Pain, B J Smith. John Wiley & Sons, New York 1973; 87–112
  • Tse H, Dutton R W. Separation of helper and suppressor T lymphocytes on a Ficoll velocity sedimentation gradient. J Exp Med 1976; 143: 1199–210
  • Dullens H FJ, Van Basten C, De Weger R A, Den Otter W. Isolation of mast cells from murine peritoneal cell populations by velocity sedimentation at unit gravity. J Immunol Methods 1981; 40: 367–72
  • Nelson R L, Bauer G E. Isolation of osteoclasts by velocity sedimentation at unit gravity. Calif Tissue Res 1977; 22: 303–13
  • Morhenn V B, Starr E D, Terrell C, et al. Separation of human skin cells by velocity sedimentation into functionally distinct fractions. J Invest Dermatol 1982; 78: 319–22
  • Enoch P G, Hemstreet G P, Fine P R. Tissue disaggregation of human kidney cells followed by purification on isokinetic and isopycnic gradients. Invest Urol 1981; 18: 345–8
  • Janss D H, Hillman E A, Malan-Shibley L B, Ben T L. Methods for the isolation and culture of normal human breast epithelial cells. Methods Cell Biol 1980; 21: 107–35
  • Forsmann W G, Lichtenberger L M, Helmstaedter V, Ito S. Studies of isolated and enriched rat antral mucosa gastrin cells. Cell Tissue Res 1979; 200: 163–77
  • Hymer W C, Snyder J, Wilfinger W, et al. Characterisation of mammotrophs separated from the human pituitary gland. J Natl Cancer Inst 1976; 57: 995–1007
  • Cohen J, Woodhams P L, Balazs R. Preparation of viable astrocytes from the developing cerebellum. Brain Res 1979; 161: 503–14
  • De Mulder P HM, Wessels J MC, Rosenbrand D A, et al. Monocyte purification with counterflow centrifugation monitored by continuous flow cytometry. J Immunol Methods 1981; 47: 31–8
  • Knook D L, Seffelaar A M, De Leeuw A M. Fat-storing cells of the rat liver: their isolation and purification. Exp Cell Res 1982; 139: 468–71
  • Greenleaf R D, Mason R J, Williams M C. Isolation of alveolar type II cells by centrifugal elutriation. In Vitro 1979; 15: 673–84
  • Devereux T R, Fouts J R. Isolation of pulmonary cells and use in studies of xenobiotic metabolism. Methods Enzymol 1981; 77: 147–54
  • Hyde C L, Childs G, Wahl L M, et al. Preparation of gonadotroph-enriched cell populations from adult rat anterior pituitary cells by centrifugal elutriation. Endocrinology 1982; 111: 1421–3
  • Keng P C, Wheeler K T, Siemann D W, Lord E M. Direct synchronisation of cells from solid tumors by centrifugal elutriation. Exp Cell Res 1981; 134: 15–22
  • Shortman K. The separation of different cell classes from lymphoid organs. I. The use of glass bead columns to separate small lymphocytes, remove damaged cells and fractionate cell suspensions. Aust J Exp Biol Med Sci 1966; 44: 271–86
  • Steinkamp J A. Multiparameter analysis and sorting of mammalian cells. Methods of cell separation, Vol. 1., N Catsimpoolas. Plenum, New York 1977; 251–306
  • Pretlow T G, Pretlow T P. Cell electrophoresis. Int Rev Cytol 1979; 61: 85–128
  • Catsimpoolas N, Griffith A L. Preparative density gradient electrophoresis and velocity sedimentation at unit gravity of mammalian cells. Methods of cell separation, Vol. 1, N Catsimpoolas. Plenum, New York 1977; 1–24
  • Hannig K, Wirth H, Meyer B H, Zeiller K. Free-flow electrophoresis. I. Theoretical and experimental investigations of the influence of mechanical and electrokinetic variables on the efficiency of the method. Hoppe-Seyler's Z Physiol Chem 1975; 356: 1209–23
  • Zeiller K, Loser R, Pascher G, Hannig K. Free-flow electrophoresis. II. Analysis of the method with respect to preparative cell separation. Hoppe-Seyler's Z Physiol Chem 1975; 356: 1225–44
  • Boltz R C, Todd P, Hammerstedt R H, . Initial studies on the separation of cells by density gradient isoelectric focusing. Cell separation methods, H Bloemendal, et al. North Holland, Amsterdam 1977; 145–55
  • Mc Guire J K, Muller T Y, Tipps R W, et al. New experimental approaches to the isoelectric fractionation of cells. J Chromatogr 1980; 194: 323–33
  • Häyry P, Nordling S, Andersson L C. Charge fractionation of lymphoid cells. In vitro methods in cell-mediated and tumor immunity, B R Bloom, J R David. Academic Press, New York 1976; 309–18
  • Plastsoucas C D, Catsimpoolas N. Separation of rat T and B lymphocytes by density gradient electrophoresis. J Immunol Methods 1979; 26: 245–51
  • Heidrich H G, Dew M E. Homogeneous cell populations from rabbit kidney cortex: proximal, distal tubule and renin-active cell isolated by free-flow electrophoresis. J Cell Biol 1977; 74: 780–8
  • Walter H A. Partition of cells in two-polymer aqueous phases: a surface affinity method for cell separation. Methods of cell separation, Vol 1, N Catsimpoolas. Plenum, New York 1977; 307–54
  • Fisher D. The separation of cells and organelles by partitioning in two-polymer aqueous phases. Biochem J 1981; 196: 1–10
  • Matsumoto U, Shibusawa Y. Surface affinity chromatographic separation of blood cells. I. Separation of human and rabbit peripheral granulocytes, lymphocytes and erythrocytes using. polyethylene glycol-bonded column packings. J Chromatogr 1980; 187: 351–62
  • Malmström P, Nelson K, Jönsson A, et al. Separation of rat leucocytes by countercurrent distribution in aqueous two-phase systems. I. Characterisation of subpopulations of cells. Cell Immunol 1978; 37: 409–21
  • Devereux T R, Fouts J R. Isolation and identification of Clara cells from rabbit lung. In Vitro 1980; 16: 958–68
  • Pohl H A. Dielectrophoresis: applications to the characterisation and separation of cells. Methods of cell separation, Vol 1, N Catsimpoolas. Plenum, New York 1977; 67–169
  • Arndt-Jovin D, Jovin T M. Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977; 25: 585–9
  • Lydon M J, Keeler K D, Thomas D B. Vital DNA staining and cell sorting by flow microfluorometry. J Cell Physiol 1980; 102: 175–81
  • Jongkind J F, Verkerk A, Visser W J, Van Dongen J M. Isolation of autofluorescent “aged” human fibroblasts by flow sorting. Exp Cell Res 1982; 138: 409–17
  • Leary J F, Finkelstein J N, Notter R H, Shapiro D L. Isolation of type II pneumocytes by laser flow cytometry. Am Rev Resp Dis 1982; 125: 326–30
  • Melville D, Paul F, Roath S. Direct magnetic separation of red cells from whole blood. Nature 1975; 255: 706
  • Paul F, Roath S, Melville D, et al. Separation of malaria-infected erythrocytes from whole blood: use of selective high-gradient magnetic separation technique. Lancet 1981; 2: 70–1
  • Owen C S, Babu U M, Cohen S W, Maurer P H. Magnetic enrichment of antibody-secreting cells. J Immunol Methods 1982; 51: 171–81
  • Grinnell F. Cellular adhesiveness and extracellular substrata. Int Rev Cytol 1978; 53: 65–144
  • Shortman K, Williams N, Jackson H, et al. The separation of different cell classes from lymphoid organs. IV. The separation of lymphocytes from phagocytes on glass bead columns, and its effect on subpopulations of lymphocytes and antibody-forming cells. J Cell Biol 1971; 48: 566–79
  • Wood P R, Simes R J, Nelson D S. Activity of mouse macrophages purified by adherence to, and removal from, a plastic surface. J Immunol Methods 1979; 28: 117–24
  • Julius M H, Simpson E, Herzenberg S A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol 1973; 3: 645–9
  • Greaves M F, Janossy G, Curtis P. Purification of human T lymphocytes using nylon fibre columns. vitro methods in cell-mediated and tumour immunity, B R. Bloom, J R David. Academic Press, New York 1976; 217–29
  • Hansen C B, Gillespie G Y, Russell S W. Isolation of T lymphocytes from disaggregated tumors, with high purity and good percentage recovery. J Natl Cancer Inst 1977; 59: 273–5
  • Jerrels T R, Dean J H, Richardson G L, Herberman R B. Depletion of monocytes from human peripheral blood mononuclear leucocytes: comparison of the Sephadex G-10 column method with other commonly used techniques. J Immunol Methods 1980; 32: 11–29
  • Ren E C. Macrophage depletion using Cytodex. J Immunol Methods 1982; 49: 105–11
  • Sjögren H O, Nilsson K, Malmström P, Axelsson B. Column separation of monocytes by adherence to gelatin beads. J Immunol Methods 1983; 56: 285–94
  • Bradley L M, Chen Y U. Cell surface markers: peanut and soybean agglutinins. Selected methods in cellular immunology, B B Mishell, S M Shiigi. W.H. Freeman & Co., San Francisco 1980; 224–7
  • Reisner Y, Kapoor N, O'Reilly R J, Good R A. Allogeneic bone marrow transplantation using stem cells fractionated by lectins. VI. In vitro analysis of human and monkey bone marrow cells fractionated by sheep red blood cells and soybean agglutinin. Lancet 1980; 2: 1320–4
  • Hellström U, Hammarström S, Dillner M L, et al. Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to Sepharose beads. Scand J Immunol 1976; 5((Suppl 5))45–55
  • Pereira M EA, Kabat E A. A versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations. J Cell Biol 1979; 82: 185–94
  • Rutishauser U S, Edelman G M. Fractionation and manipulation of cells with chemically modified fibres and surfaces. Methods of cell separation, Vol. 1, N Catsimpoolas. Plenum, New York 1977; 193–228
  • Phillips S G, Kabat E A, Miller O J. Nylon fibre affinity selection of red blood cells and tissue culture cells on the basis of cell surface determinants. Exp Cell Res 1980; 127: 361–71
  • Boldt D H, Lyons R D. Fractionation of human lymphocytes with plant lectins. I. Structural and functional characteristics of lymphocyte subclasses isolated by an affinity technique using Lens culinaris lectin. Cell Immunol 1979; 43: 82–93
  • Nakamura T, Tanimoto K, Nakano K, Horiuchi Y. Isolation of human suppressor T cells by peanut agglutinin. Int Arch Allergy Appl Immunol 1982; 68: 338–41
  • Kronick P L. Magnetic microspheres in cell separation. Methods of cell separation, Vol. 3, N Catsimpoolas. Plenum, New York 1980; 115–39
  • Wahl S M, Rosenstreich D L, Oppenheim J J. Separation of human lymphocytes by E rosette sedimentation. In vitro methods in cell-mediated and tumor immunity, B R Bloom, J R David. Academic Press, New York 1976; 231–43
  • Bianco C, Patrick R, Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterisation. J Exp Med 1970; 132: 702–20
  • Parish C R, Hayward J A. The lymphocyte surface. II. Separation of Fc receptor, C′3 receptor and surface immunoglobulin-bearing lymphocytes. Proc Roy Soc Lond (Biol) 1974; 187: 65–81
  • Kronick P L, Campbell G L, Joseph K. Magnetic microspheres prepared by redox polymerisation used in a cell separation based on gangliosides. Science 1978; 200: 1074–6
  • Wigzell H. Enrichment or depletion of surface immunoglobulin-coated cells using anti-immunoglobulin antibodies and glass or plastic bead columns. In vitro methods in cell-mediated and tumor immunity, B R Bloom, J R David. Academic Press, New York 1976; 245–53
  • Platsoucas C D, Catsimpoolas N. Biological methods for the separation of lymphoid cells. Methods of cell separation, Vol. 3, N Catsimpoolas. Plenum, New York 1980; 157–200
  • Braun R, Teute H, Kirchner H, Munk K. Rapid separation of T cell subpopulations with monoclonal antibodies and affinity chromatography. J Immunol Methods 1982; 54: 251–8
  • Lewis G K, Kamin R. Cell surface markers: separation of T and B cells using plastic surfaces coated with anti-immunoglobulin antibodies (“panning”). Selected methods in cellular immunology, B B Mishell, S M Shiigi. W.H. Freeman & Co., San Francisco 1980; 227–34
  • Baran M M, Allen D M, Russell S R, et al. Cell sorting using a universally applicable affinity chromatography matrix: solid-phase anti-fluorescein isothiocyanate antibody. J Immunol Methods 1982; 53: 321–34
  • Clark N WT, Parkhouse R ME, Simmonds R G. Positive and negative selection of cells by hapten-modified antibodies. J Immunol Methods 1982; 51: 167–70
  • Basch R S, Berman J W, Lakow E. Cell separation using positive immunoselective techniques. J Immunol Methods 1983; 56: 269–80
  • Ghetie V, Mota G, Sjöquist J. Separation of cells by affinity chromatography on SpA-Sepharose 6MB. J Immunol Methods 1978; 21: 133–41
  • Parish C R, Kirov S M, Bowern N, Blanden R V. A one-step procedure for separating mouse T and B lymphocytes. Eur J Immunol 1974; 4: 808–15
  • Egeland T, Lea T. A rapid rosette technique for quantitation and separation of mononuclear Cell subsets using monoclonal antibodies. J Immunol Methods 1982; 55: 213–9
  • Wofsy L, Henry C, Cammisuli S. Hapten-sandwich labelling of cell surface antigens. ContempTop Mol Immunol 1978; 7: 215–37
  • Ghetie V, Stalenheim G, Sjöquist J. Cell separation by staphylococcal protein A-coated erythrocytes. Scand J Immunol 1975; 4: 471–7
  • Ljungstedt L, Ekman B, Sjöholm I. Detection and separation of lymphocytes with specific surface receptors by using microparticles. Biochem J 1978; 170: 161–5
  • Lindmark R, Larsson E, Nilsson K, Sjöquist J. Polyacrylamide microbeads with entrapped protein A from S. aureus for cell separation. Acta Pathol Microbiol Scand (C) 1982; 90: 117–22
  • Molday R S, Yen S PS, Rembaum A. Application of magnetic microspheres in labelling and separation of cells. Nature 1977; 268: 437–8
  • Antoine J, Ternynck T, Rodrigot M, Avrameas S. Lymphoid cell fractionation on magnetic polyacrylamide-agarose beads. Immunochemistry 1978; 15: 443–52
  • Widder K. J, Senyei A E, Ovadia H, Paterson P Y. Magnetic protein A microspheres: a rapid method for cell separation. Clin Immunol Immunopathol 1979; 14: 395–400
  • Rembaum A, Dreyer W J. Immunomicrospheres: reagents for cell labelling and separation. Science 1980; 208: 364–8
  • Smolka A JK, Margel S, Nerren B H, Rembaum A. Electrophoretic cell separation by means of microspheres. Biochim Biophys Acta 1979; 588: 246–55
  • Hansen E, Hannig K. Antigen-specific electrophoretic cell separation: isolation of human B and T lymphocyte subpopula-tions by free-flow electrophoresis after reactions with antibodies. J Immunol Methods 1982; 51: 197–208
  • Lakow E, Basch R S. Positive immunoselection using antibody-enzyme complexes. J Immunol Methods 1981; 44: 135–51
  • Arndt-Jovin D, Jovin T M. Automated cell sorting with flow systems. Ann Rev Biophys Bioeng 1978; 7: 527–58
  • Miller R G, Lalande M E, McCutcheon M J, et al. Review: usage of the flow cytometer-cell sorter. J Immunol Methods 1981; 47: 13–24
  • Loken M R, Stall A M. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods 1982; 50: R85–112
  • Bradley L M, North J, Swain S L, Chan E L. Functional separation by inactivation of proliferating cells. Selected methods in cellular immunology, B B Mishell, S M Shiigi. W.H. Freeman & Co., San Francisco 1980; 235–44
  • Lee K C. Adherence: carbonyl iron powder. Selected methods in cellular immunology, B B Mishell, S M Shiigi. W.H. Freeman & Co., San Francisco 1980; 179–81
  • Allison A C. Fluorescence microscopy of lymphocytes and mononuclear phagocytes and the use of silica to eliminate the latter. In vitro methods in cell-mediated and tumor immunity, B R Bloom, J R David. Academic Press, New York 1976; 395–404
  • Haas W, Von Boehmer H. Techniques for separation and selection of antigen-specific lymphocytes. Curr Top Microbiol Immunol 1978; 84: 1–120
  • Langdon W Y, Holt P G, Shellam G R. Antigen-pulsed macrophage monolayers as specific immunoabsorbents: selective absorption of murine T cells committed to soluble protein antigen. Immunology 1981; 43: 555–62

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