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Scandinavian Journal of Clinical and Laboratory Investigation Volume 12, 1960 - Issue 2
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Original Article

Phosphatase Assay in Serum with P-Nitrophenyl Phosphate as Substrate

B. Laurent Chemical Central Laboratory, Sabbatsherg Hospital, Stockholm, Sweden
&
B. Norberg Chemical Central Laboratory, Sabbatsherg Hospital, Stockholm, Sweden
Pages 154-157 | Received 03 Oct 1959, Published online: 08 Jul 2009

References

  • Bessey O. A., Lowry O. H., Brock M. J. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J. Biol. Chem. 1946; 164: 521
  • Buch I., Buch H. An improved King and Armstrong method for the determination of phosphatase activity in blood serum. Acta med. Scandinav. 1939; 101: 211
  • Fishman W. H., Lerner F. A method for estimating serum acid phosphatase of prostatic origin. J. Biol. Chem. 1953; 200: 89
  • Hofman T. Studies on casein 2. The action of phosphatases on caseins and low-molecular-weight phosphates. Biochem. J. 1958; 69: 139
  • King E. J., Armstrong A. R. Convenient method for determining serum and bile phosphatase activity. Canad. M. A. J. 1934; 31: 376
  • Kilsheimer G. S., Axelrod B. Inhibition of prostatic acid phosphatase by a-hydroxycarb-oxylic acids. J. Biol. Chem. 1957; 227: 879
  • Jacobsson K. The determination of tartrate inhibited phosphatases in serum with p-nitro-phenyl phosphatase. Scandinav. J. Clin. & Lab. Investigation, To be published
  • Ohmori Y. Über die Phosphomonoesterase. Enzymologia 1937; 4: 217
  • Singer M. F., Fruton J. S. Some properties of beef splen phosphoamidase. J, Biol, Chem. 1957; 229: 111
  • Sundarajan T. A., Sarma P. S. Substrate specificity of phosphoprotein phosphatase from spleen. Biochem. J. 1959; 71: 537

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