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Scandinavian Journal of Clinical and Laboratory Investigation Volume 12, 1960 - Issue 2
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Original Article

Microdetermination of Protein by Determining the Protein Bound Copper of the Biuret Complex with Oxalyldihydrazide

M. Kekki Wihuri Research Institute, Helsinki, Finland
&
P. Siltanen Wihuri Research Institute, Helsinki, Finland
Pages 235-238 | Received 14 Dec 1959, Published online: 08 Jul 2009

References

  • Gaa J. Micro biuret method for protein determination. Determination of total protein in cerebrospinal fluid. Scandinav. J. Clin. & Lab. Investigation 1953; 5: 218
  • Gran G. The use of oxalyldihydrazide in a new reaction for the spectrophotometric microdeter-mination of copper. An. Chim. Acta 1956; 14: 150
  • Kekki M. Microdetermination of amino nitrogen as copper complexes. A modification for plasma and urine. Scandinav. J. Clin. & Lab. Investigation 1959; 11: 311
  • Mehl J. The biuret reaction of proteins in the presence of ethylene glycol. J. Biol. Chem. 1945; 157: 173
  • Nilsson G. Pri oksalilhidrazido (I). Scienca Revuo 1952; 3: 151
  • Nilsson G. Über eine neue Farbreaktion des Oxaldihydrazids. Ztschr. f. anal. Chem. 1956; 153: 11
  • Siltanen P., Kekki M. Determination of protein by the biuret reaction using cupric hydroxide suspension reagent. Scandinav. J. Clin. & Lab. Investigation 1960; 12: 228
  • Stark G. R., Dawson C. R. Spectrophotometric microdetermination of copper in copper oxidases using oxalyldihydrazide. Anal. Chem. 1958; 30: 191
  • Stiff H. New colorimetric method for the determination of serum protein. J. Biol. Chem. 1949; 177: 179
  • Turkington R. W., Tracy F. M. Spectrophotometric determination of ultrainicro amounts of copper with 1,5-diphenyl-carbohydrazide. Anal. Chem. 1958; 30: 1699

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