Advanced search
Publication Cover
Scandinavian Journal of Clinical and Laboratory Investigation Volume 38, 1978 - Issue 7
Submit an article Journal homepage
5
Views
6
CrossRef citations to date
0
Altmetric
Original Article

Primary structure of human intrinsic factor: progress report on cyanogen bromide fragmentation

Ebba Nexø Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400, Copenhagen; Department of Clinical Chemistry, ML-4051, Rigshospitalet, DK-2100, Copenhagen; The Danish Institute of Protein Chemistry, DK-2970, Hørsholm, Denmark
,
Henrik Olesen Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400, Copenhagen; Department of Clinical Chemistry, ML-4051, Rigshospitalet, DK-2100, Copenhagen; The Danish Institute of Protein Chemistry, DK-2970, Hørsholm, Denmark
,
Marianne Rye Hansen Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400, Copenhagen; Department of Clinical Chemistry, ML-4051, Rigshospitalet, DK-2100, Copenhagen; The Danish Institute of Protein Chemistry, DK-2970, Hørsholm, Denmark
,
Ditlef Bucher Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400, Copenhagen; Department of Clinical Chemistry, ML-4051, Rigshospitalet, DK-2100, Copenhagen; The Danish Institute of Protein Chemistry, DK-2970, Hørsholm, Denmark
&
Johannes Thomsen Department of Clinical Chemistry, Bispebjerg Hospital, DK-2400, Copenhagen; Department of Clinical Chemistry, ML-4051, Rigshospitalet, DK-2100, Copenhagen; The Danish Institute of Protein Chemistry, DK-2970, Hørsholm, Denmark
Pages 649-653 | Received 28 Feb 1978, Accepted 16 May 1978, Published online: 08 Jul 2009

References

  • Allen R. H., Mehlman C. S. Isolation of gastric vitamin B12-binding proteins using affinity chromatography. J. biol. Chem. 1973; 248: 3660
  • Brauer A. W., Margolies M. N., Haber E. The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides. Biochemistry 1975; 14: 3029
  • Christensen J. M., Hippe E., Olesen H., Rye M., Haber E., Lee L., Thomsen J. Purification of human intrinsic factor by affinity chromatography. Biochim. biophys. Acta 1973; 303: 319
  • Edman P., Begg G. A protein sequenator. Europ. J. Biochem. 1967; 1: 80
  • McFarlane A. S. Efficient trace-labelling of proteins with iodine. Nature 1958; 182: 53
  • Hugh T. E., Moore S. Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. J. biol. Chem. 1972; 247: 2828
  • Katz M., Mehlman C. S., Allen R. H. Isolation and characterization of an abnormal human intrinsic factor. J. clin. Invest. 1974; 53: 1274
  • Konigsberg W. Reduction of disulfide bonds in proteins with dithiothreitol. Methods in Enzymology, S. P. Colowick, N. O. Kaplan. Academic Press, New York 1972; Vol. XXV B.: 785
  • Kulbe K. D. Micropolyamide thin-layer chromatography of phenyithiohydantoin amino acids (PTH) at subnanomolar level. A rapid microtechnique for simultaneous multisample identification after automated Edman degradations. Analyt. Biochem. 1974; 59: 564
  • Mendez E., Lai C. Y. Regeneration of amino acids from thiazolinones formed in the Edman degradation. Analyt. Biochem. 1975; 68: 47
  • Moore S., Stein W. H. Chromatographic determination of amino acids by the use of automatic recording equipment. Methods in Enzymology, S. P. Colowick, N. O. Kaplan. Academic Press, New York 1963; Vol. VI: 819
  • Nexø E. A new principle in biospecific affinity chromatography used for purification of cobalamin-binding proteins. Biochim. biophys. Acta 1975; 379: 189
  • Olesen H., Nexo E., Lous P., Thomsen J., Bucher D. Amino terminal sequence of human intrinsic factor. Scand. J. clin. Lab. Invest. 1976; 36: 527
  • Pisano J. J. Analysis of amino acid phenylthio-hydantoins by gas chromatography and high performance liquid chromatography. Protein Sequence Determination, 2nd Ed., S. B. Needle-Man. Springer-Verlag, Berlin 1975; 280
  • Spencer R. L., Wold F. A new convenient method for estimation of total cystine-cysteine in proteins. Analyt. Biochem. 1969; 32: 185
  • Tarr G. E. A general procedure for the manual sequencing of small quantities of peptides. Analyt. Biochem. 1975; 63: 361
  • Visuri K., Gräsbeck R. Human intrinsic factor, isolation by improved conventional methods and properties of the preparation. Biochim. biophys. Acta 1973; 310: 508

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.