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Scandinavian Journal of Clinical and Laboratory Investigation Volume 41, 1981 - Issue 2
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Original Article

A sensitive and simplified receptor assay for 1,25(OH)2 vitamin D

J. T. Dabek Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
,
M. Härkönen Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
&
H. Adlercreutz Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
Pages 151-158 | Received 19 May 1980, Accepted 09 Dec 1980, Published online: 08 Jul 2009

References

  • Björkhem I., Holmberg I., Kristiansen T., Pedersen J. I. Assay of 1,25-dihydroxyvitamin D3 by isotope dilution-mass fragmentography. Clin. Chem 1979; 25: 584
  • Brumbaugh P. F., Haussler D. H., Bressler R., Haussler M.R. Radioreceutor assay for 1α,25-dihydroxyvitamin D3. Science 1974; 183: 1089
  • Brumbaugh P. F., Haussler D. H., Bursa K. M., Haussler M. R. Filter assay for 1α,25-dihydroxy-vitamin D3. Utilization of the hormones target tissue chromatin receptor. Biochemistry 1974; 11: 4091
  • Caldas A. E., Gray R. W., Lemann I. The simultaneous measurement of vitamin D metabolites in plasma: studies in healthy adults and in patients with calcium nephrolithiosis. J. Lab. clin. Med 1970; 91: 840
  • Clemens T. L., Hendy G. N., Papapoulos S. E., Fraher L. J., Care A. D., O'Riordan J. L.H. Measurement of 1,25-dihydroxycholecalciferol in man by radioimmunoassay. Clin. Endocr 1979; 11: 225
  • Dabek J. T. Plasma profile of hydroxylated vitamin D metabolites: methods and results in normals for spring-winter in Southern Finland. Ann. Clin. Res 1980; 12: 17
  • Dabek J. T., Härkönen M., Adlercreutz H. Application of the law of mass action to design and performance of receptor assays for 1,25(OH)2 vitamin D. Scand. J. clin. Lab. Invest 1981, In press
  • Eisman J. A., Hamstra A. J., Kream B. E., De Luca F. A sensitive, precise and convenient method for determination of 1,25-dihydroxy-vitamin D in human plasma. Arch. Biochem. Biophys 1976; 176: 235
  • Lambert P. W., Syverson B. J., Arnaud C. D., Spelsberg T. C. Isolation and quantitation of endogenous vitamin D and its physiologically important metabolites in human plasma by high pressure liquid chromatography. J. Steroid Biochem 1977; 8: 929
  • Lund B., Lund B., Sørensen O. H. Measurement of circulating 1,25-dihydroxyvitamin D in man. Changes in serum concentrations during treatment with 1α-hydroxycholecalciferol. Acta endocrinol. Kbh 1979; 91: 338
  • Stern P. H., Hamstra A. J., De Luca H. F., Bell N. H. A bioassay capable of measuring 1 picogram of 1,25-dihydroxyvitamin D3. J. clin. Endocr. Metab 1978; 46: 891
  • Taylor C. M., Hann J., St. John J., Wallace J.E., Mawer E.B. 1,25-Dihydroxycholecalciferol in human serum and its relationship with other metabolites of vitamin D-3. Clin. chim. Acta 1979; 96: 1

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