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Scandinavian Journal of Clinical and Laboratory Investigation Volume 44, 1984 - Issue 3
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Original Article

Technical Note: Extraction from bone powder: Collagen, calcium and phosphate

L. Tougaard Research Laboratory of Medical Department, C. Glostrup University Hospital, DK-2600, Glostrup, Denmark
,
N. Tøffner Clausen Research Laboratory of Medical Department, C. Glostrup University Hospital, DK-2600, Glostrup, Denmark
,
K. Grunnet Research Laboratory of Medical Department, C. Glostrup University Hospital, DK-2600, Glostrup, Denmark
&
J. Thode Medical Department, C. Herlev University Hospital, DK-2730, Herlev, Denmark
Pages 267-269 | Received 25 Nov 1983, Accepted 19 Dec 1983, Published online: 08 Jul 2009

References

  • Asthon B. A., Triffitt J. T., Herring G. M. Isolation and partial characterization of a glycoprotein from bovine cortical bone. Eur. J. Biochem. 1974; 45: 525
  • Cohen-Solal L., Glimcher M. J. Organic phosphorus distribution in chicken bone matrix. Ann. Biol. Anim. Biochem. Biophys. 1978; 18: 99
  • Herring G. M. The organic matrix of bone. The Biochemistry and Physiology of Bone, G. H. Bourne. Academic Press, New York 1972
  • Herring G. M., Ashton B. A., Chipperfiels A. R. The isolation of soluble proteins, glycoproteins and proteoglycans from bone. Prep. Biochem. 1974; 4: 179
  • Herring G. M. Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Calcif. Tissue Res. 1977; 24: 29
  • Kiviranta I., Tammi M., Lappalainen R., Kuusela T., Helminen H. J. The range of calcium extraction during EDTA decalcification from thin bone slices as assessed with atomic absorption spectrophotometry. Histochem. 1980; 68: 199
  • Kuboki Y., Shimokawa H., Ono T., Sasaki S. Detection of collagen degradation products in bone. Calcif. Tissue Res. 1973; 12: 303
  • Spector A. R., Glimcher M. J. The extraction and characterization of soluble anionic phospho-proteins from bone. Biochem. biophys. acta. 1972; 263: 593
  • Tougaard L. The degree of mineralization in bone tissue. The phosphorus/hydroxyproline ratio determined on small amounts of bone tissue. Scand. J. din. Lab. Invest. 1973; 23: 351

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