12
Views
5
CrossRef citations to date
0
Altmetric
Original Article

A simple and rapid method for purification of rat haptoglobin for production of antiserum

, &
Pages 565-569 | Received 13 Mar 1991, Accepted 20 May 1991, Published online: 08 Jul 2009

References

  • Putnam FW. Haptoglobin. The Plasma Proteins, FW Putnam. Academic Press, New York 1975; Vol 2.: 1–50
  • Giblett E. Haptoglobin. Structure and Function of Plasma Proteins, AC Allison. Plenum Press, London 1974; Vol 1.: 55–72
  • Malchy B, Rorstad O, Dixon GH. The half-molecule of haptoglobin: studies on the product obtained by the selective cleavage of a haptoglobin disulphide. Can J Biochem 1973; 51: 265–73
  • Hanley J M, Haugen T H, Heath EC. Biosynthesis and processing of rat haptoglobin. J Biol Chem 1983; 258: 7858–69
  • Misumi Y, Tanaka Y, Ikehara Y. Biosynthesis, intracellular processing and secretion of haptoglobin in cultured rat hepatocytes. Biochem Biophys Res Commun 1983; 114: 729–36
  • Gianazza E, Arnaud P. Chromatography of plasma proteins on immobilized Cibacron Blue F3GA: mechanism of the molecular interaction. Biochem J 1982; 201: 129–36
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–85
  • Lowry O H, Rosebrough N J, Farr A L, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951; 193: 265–75
  • Nilsson LA. Immunoelectrophoresis. Handbook in Immunoprecipitation-in-Gel Techniques (Scand J Immunol, Vol. 17, Suppl. 10)., NH Axelsen. Blackwell Scientific Publications, Oxford 1983; 71–6
  • Grubb AO. Crossed Immunoelectrophoresis. Handbook in Immunopre-cipitation-in-Gel Techniques. (Scand J Immunol, Vol. 17, Suppl. 10)., NH Axelsen. Blackwell Scientific Publications, Oxford 1983; 113–24
  • Mancini G, Carbonara A, Heremans O. J.F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochem 1965; 2: 235–54
  • Rademacher B E, Steele WJ. A general method for the isolation of haptoglobin 1–1,2–1 and 2–2 from human plasma. Anal Biochem 1987; 160: 119–26
  • Subramanian S. Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes. Crit Rev Biochem 1984; 16: 169–204

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.