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Original Article

A simplified high-performance liquid chromatographic method for determination of vitamin D3, 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human serum

Pages 177-182 | Received 23 May 1991, Accepted 03 Oct 1991, Published online: 08 Jul 2009

References

  • Haussler M R, Brickman A S. Vitamin D: Metabolism, actions and disease states. Disorders of Mineral Metabolism. Vol. II: Calcium Physiology, F Bronner, J W Coburn. Academic Press, New York 1982; 359–431
  • Holmes R P, Kummerow F A. The relationship of adequate and excessive intake of vitamin D to health and disease. J Am Coll Nutr 1983; 2: 173–99
  • Aksnes L, Rødland O, Aarskog D. Serum levels of vitamin D, and 25-hydroxyvitamin D3 in elderly and young adults. Bone Mineral 1988; 3: 351–7
  • Holmberg I, Aksnes L, Berlin T, Lindbäck B, Zemgals J, Lindeke B. Absorption of a pharmacological dose of vitamin D3: Comparison of arachis oil and a medium chain triglyceride as lipid vehicle. Biopharm Drug Disp 1990; 11: 807–15
  • Bouillon R, van Kerkhove P, De Moor P. Measurement of 25-hydroxyvitamin D3 in serum. Clin Chem 1976; 22: 364–8
  • Aksnes L. An improved competitive proteinbinding assay for 25-hydroxy vitamin D. Scand J Clin Lab Invest 1978; 38: 677–86
  • Aksnes L. Quantitation of the main metabolites of vitamin D in a single serum sample. I. Extraction, separation and purification of metabolites. Clin Chim Acta 1980; 104: 133–46
  • Adams J S, Clemens T L, Holick M F. Sep-pak preparative chromatography for vitamin D and its metabolites. J Chromatogr Biomed Appl 1981; 226: 198–201
  • Dabek J T, Härkönen M, Wahlroos ö, Adlercreutz H. Assay for plasma 25-hydroxy-vitamin D2 and 25-hydroxyvitamin D, by “high-performance” liquid chromatography. Clin Chem 1981; 27: 1346–51
  • Aksnes L. Quantitation of the main metabolies of vitamin D in a single serum sample. II. Determination by UV-absorption and competitive protein binding assays. Clin Chim Acta 1980; 104: 147–59
  • Hummer L, Nilas L, Tjellesen L, Christiansen C. A selective and simplified radioimmunoassay of 25-dihydroxyvitamin D3. Scand J Clin Lab Invest 1984; 44: 163–7
  • Eisman J A, Shepard R M, DeLuca H F. Determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma using high-pressure liquid chromatography. Anal Biochem 1977; 80: 298–305
  • Bouillon R, Van Herck E, Jans I, Tan B K, Van Baelen H, DeMoor P. Two direct (nonchromatographic) assays for 25-hydroxyvitamin D. Clin Chem 1984; 30: 1731–6
  • Holis B W, Napoli J L. Improved radioimmunoassay for vitamin D and its use in assessing vitamin D status. Clin Chem 1985; 31: 1815–9
  • Chen T C, Turner A K, Holick M F. Methods for the determination of the circulating concentration of 25-hydroxyvitamin D. J Nutr Biochem 1990; 1: 315–9
  • Lindbäck B, Berlin T, Björkhem I. Evaluation of three commercial kits and one liquid-chromatographic method for determining concentrations of 25-hydroxyvitamin D, in serum. Clin Chem 1987; 33: 1226–7
  • Jones G. Assay of vitamin D2 and D3 and 25-hydroxyvitamin D2 and D3 in human plasma by high-performance liquid chromatography. Clin Chem 1978; 24: 287–93
  • Reinhart T A, Horst R L, Orf J W, Hollis B W. A microassay for 1,25-dihydroxyvitamin D not requiring high performance liquid chromatography: Application to clinical studies. J Clin Endocrinol Metab 1984; 58: 91–8
  • Aksnes L, Rødland O, Ødegaard O R, Bakke K J, Aarskog D. Serum levels of vitamin D metabolites in the elderly. Acta Endocrinol (Copenh) 1989; 121: 70–33
  • Markestad T, Halvorsen S, Seeger Halvorsen K, Aksnes L, Aarskog D. Plasma concentrations of vitamin D metabolites before and during treatment of vitamin D deficiency rickets in children. Acta Paediatr Scand 1984; 73: 225–31
  • Adams J S, Clemens T L, Parrish J A, Holick M F. Vitamin D synthesis and metabolism after ultraviolet irradiation of normal and vitamin D-dcficient subjects. New Engl J Med 1982; 306: 722–5

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