Advanced search
Publication Cover
Scandinavian Journal of Gastroenterology Volume 32, 1997 - Issue 4
Submit an article Journal homepage
17
Views
2
CrossRef citations to date
0
Altmetric
Original Article

Variations in Pigment and Carbohydrate Content of Gallbladder Bile Affect Accurate Quantitation of Total Protein When Using the Fluorescamine Method

S. Ginanni Corradini Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
G. Yamashita Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
H. Nuutinen Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
A. Chernosky Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
C. Williams Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
L. Hays Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
M. L. Shiffman Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
R. M. Walsh Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
J. Svanvik Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
P. Della Guardia Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
,
L. Capocaccia Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
&
R. T. Holzbach Gastrointestinal Research Unit, Depts. of Gastroenterology and General Surgery, Cleveland Clinic Foundation, Cleveland, Ohio; Dept. of Gastroenterology, Virginia Commonwealth University, Richmond, Virginia, USA; Dept. of Surgery, University of Linköping, Linköping, Sweden; Dept. of Clinical Medicine, Division of Gastroenterology, La Sapienza University, Rome, Italy
 show all
Pages 340-349 | Received 28 Jul 1996, Accepted 31 Dec 1996, Published online: 08 Jul 2009

References

  • Ostrow JD. Absorption by the gallbladder of bile salts, sulfobromophthalein, and iodipamide. J Lab Clin Med 1969; 74: 482–94
  • Ostrow JD. Absorption of organic compounds by the injured gallbladder. J Lab Clin Med 1971; 78: 255–64
  • Svanvik J, Allen B, Pellegrini C, Bernhoft R, Way L. Variations in concentrating function of the gallbladder in the conscious monkey. Gastroenterology 1984; 86: 919–25
  • Svanvik J. Role of gallbladder in modifying hepatic bile composition. Hepatic transport and bile secretion physiology and pathophysiology, N Tavoloni, PD Berk. Raven Press, New York 1994; 607–18
  • Holan KR, Holzbach RT, Hermann RE, Cooperman AM, Claffey WJ. Nucleation time: a key factor in the pathogenesis of cholesterol gallstone disease. Gastroenterology 1979; 77: 611–7
  • Sahlin S, Danielsson A, Angelin B, Reihnér E, Henriksson R, Einarsson K. Mucin in gall bladder bile of gall stone patients: influence of treatment with chenodeoxycholic acid and ursodeoxycholic acid. Gut 1988; 29: 1506–10
  • Gallinger S, Harvey PRC, Petrunka CN, Ilson RG, Strasberg SM. Biliary proteins and the nucleation defect in cholesterol cholelithiasis. Gastroenterology 1987; 92: 867–75
  • Strasberg SM, Toth JL, Gallinger S, Harvey PRC. High protein and total lipid concentration are associated with reduced metastability of bile in an early stage of cholesterol gallstone formation. Gastroenterology 1990; 98: 739–46
  • Chijiiwa K, Hirota I, Noshiro H. High vesicular cholesterol and protein in bile are associated with formation of cholesterol but not pigment gallstones. Dig Dis Sci 1993; 38: 161–6
  • Swobodnik W, Wenk H, Janowitz P, Hagert N, Kratzer W, Berghold J. Total biliary protein, mucus glycoproteins, cyclic-AMP, and apolipoproteins in the gallbladder bile of patients with cholesterol stones and stone-free controls. Scand J Gastroenterol 1991; 26: 771–8
  • Harvey PRC, Upadhya GA, Toth JL, Strasberg SM. Fluorometric assay of protein in native human bile. Clin Chim Acta 1989; 183: 147–54
  • Tera H. Stratification of human gallbladder bile in vivo. Acta Chir Scand 1960; 256: 1–85, Suppl
  • Strasberg SM, Harvey PRC, Hofmann AF. Bile sampling, processing and analysis in clinical studies. Hepatology 1990; 12: 176, S–82S.
  • Turley SD, Dietschy JM. Re-evaluation of the 3α-hydroxysteroid dehydrogenase assay for total bile acids in bile. J Lipid Res 1978; 19: 924–8
  • Takayama M, Itoh S, Nagasaki T, Tanimizu I. A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta 1977; 79: 93–8
  • Gurantz D, Laker MF, Hofmann AF. Enzymatic measurement of choline-containing phospholipids in bile. J Lipid Res 1981; 22: 373–6
  • Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. J Clin Chem 1974; 20: 470–5
  • Yamazaki K, Powers SP, LaRusso NF. Biliary proteins: assessment of quantitative techniques and comparison in gallstone and nongallstone subjects. J Lipid Res 1988; 29: 1055–63
  • Ginanni Corradini S, Nuutinen H, Schwarzendrube J, Williams C, Chernosky A, Chandler K. Protein concentration in gallbladder bile of cholesterol gallstone patients and controls: comparative results with the use of different quantitative methods [abstract]. Gastroenterology 1993; 104: 907, A.
  • Busch N, Tokumo H, Cottle A, Mehraban F, Holzbach RT. Problems in the measurement of biliary proteins with the fluorescamine assay [abstract]. Hepatology 1988; 8: 1371, A.
  • Harlow E, Lane D. Antibodies a laboratory manual. Cold Spring Harbor Laboratory, New York 1988
  • Mantle M, Allen A. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain. Biochem Soc Trans 1978; 6: 607–9
  • Neder J, Wasserman W, Kutner MH. Applied linear statistical models. 3rd ed. Irwin Pub Co, Homewood (IL) 1990
  • Woolson RF. Statistical methods for the analysis of biomedical data. John Wiley & Sons, New York 1987
  • Wessel D, Flügge UI. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 1984; 138: 141–3
  • Ostrow JD. Absorption of bile pigments by the gall bladder. J Clin Invest 1967; 46: 2035–52
  • Shiffman ML, Shamburek RD, Schwartz CC, Sugerman HJ, Kellum JM, Moore EW. Gallbladder mucin, arachidonic acid, and bile lipids in patients who develop gallstones during weight reduction. Gastroenterology 1993; 105: 1200–8
  • Wong K, Ekberg O, Laufer I, Malet PF, Arger P. Nonvisualized gallbladder on oral cholecystography: implications for lithotripsy. Gastrointest Radiol 1990; 15: 126–8
  • Rome Group for the Epidemiology and Prevention of Cholelithiasis. Radiologic appearance of gallstones and its relationship with biliary symptoms and awareness of having gallstones. Observations during epidemiology studies. Dig Dis Sci 1987; 32: 349–53
  • Marzio L, Innocenti P, Genovesi N, Di Felice F, Napolitano AM, Constantini R. Role of oral cholecystography, realtime ultrasound, and CT in evaluation of gallstones and gallbladder function. Gastrointest Radiol 1992; 17: 257–61

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.