318
Views
13
CrossRef citations to date
0
Altmetric
Rapid Communication

Impact of Pseudomonas aeruginosa quorum sensing on cellular wound healing responses in vitro

, &
Pages 615-619 | Received 14 Nov 2011, Accepted 22 Dec 2011, Published online: 19 Feb 2012

References

  • Gjodsbol K, Christensen JJ, Karlsmark T, Jorgensen B, Klein BM, Krogfelt KA. Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J 2006;3: 225–31.
  • Bjarnsholt T, Kirketerp-Moller K, Jensen PO, Madsen KG, Phipps R, Krogfelt K, . Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen 2008; 16:2–10.
  • James GA, Swogger E, Wolcott R, Pulcini E, Secor P, Sestrich J, . Biofilms in chronic wounds. Wound Repair Regen 2008;16:37–44.
  • Alhede M, Bjarnsholt T, Jensen PO, Phipps RK, Moser C, Christophersen L, . Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes. Microbiology 2009;155(Pt 11): 3500–8.
  • Bjarnsholt T, Givskov M. The role of quorum sensing in the pathogenicity of the cunning aggressor Pseudomonas aeruginosa. Anal Bioanal Chem 2007;387:409–14.
  • Rumbaugh KP, Griswold JA, Iglewski BH, Hamood AN. Contribution of quorum sensing to the virulence of Pseudomonas aeruginosa in burn wound infections. Infect Immun 1999;67:5854–62.
  • Andersen AS, Jorgensen B, Bjarnsholt T, Johansen H, Karlsmark T, Givskov M, Krogfelt KA. Quorum sensing regulated virulence factors in Pseudomonas aeruginosa are toxic to Lucilia sericata maggots. Microbiology 2010; 156(Pt 2):400–7.
  • Nakagami G, Sanada H, Sugama J, Morohoshi T, Ikeda T, Ohta Y. Detection of Pseudomonas aeruginosa quorum sensing signals in an infected ischemic wound: an experimental study in rats. Wound Repair Regen 2008;16: 30–6.
  • Hentzer M, Wu H, Andersen JB, Riedel K, Rasmussen TB, Bagge N, . Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO J 2003;22: 3803–15.
  • Davies DG, Parsek MR, Pearson JP, Iglewski BH, Costerton JW, Greenberg EP. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 1998;280: 295–8.
  • [Hoiby N, Krogh JH, Moser C, Song Z, Ciofu O, Kharazmi A. Pseudomonas aeruginosa and the in vitro and in vivo biofilm mode of growth. Microbes Infect 2001;3: 23–35.
  • Wu H, Song Z, Hentzer M, Andersen JB, Heydorn A, Mathee K, . Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa. Microbiology 2000;146(Pt 10):2481–93.
  • Agren MS, Werthen M. The extracellular matrix in wound healing: a closer look at therapeutics for chronic wounds. Int J Low Extrem Wounds 2007;6:82–97.
  • Steffensen B, Hakkinen L, Larjava H. Proteolytic events of wound-healing—coordinated interactions among matrix metalloproteinases (MMPs), integrins, and extracellular matrix molecules. Crit Rev Oral Biol Med 2001;12: 373–98.
  • Stephens P, Wall IB, Wilson MJ, Hill KE, Davies CE, Hill CM, . Anaerobic cocci populating the deep tissues of chronic wounds impair cellular wound healing responses in vitro. Br J Dermatol 2003;148:456–66.
  • Bjarnsholt T, Jensen PO, Burmolle M, Hentzer M, Haagensen JA, Hougen HP, . Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent. Microbiology 2005;151:373–83.
  • Pearson JP, van Delden C, Iglewski BH. Active efflux and diffusion are involved in transport of Pseudomonas aeruginosa cell-to-cell signals. J Bacteriol 1999;181: 1203–10.
  • Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007;2:329–33.
  • Geback T, Schulz MM, Koumoutsakos P, Detmar M. TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays. Biotechniques 2009;46:265–74.
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55–63.
  • Wolcott RD, Rhoads DD. A study of biofilm-based wound management in subjects with critical limb ischaemia. J Wound Care 2008;17:145–8, 150–2, 154–5.
  • de Bentzmann S, Polette M, Zahm JM, Hinnrasky J, Kileztky C, Bajolet O, . Pseudomonas aeruginosa virulence factors delay airway epithelial wound repair by altering the actin cytoskeleton and inducing overactivation of epithelial matrix metalloproteinase-2. Lab Invest 2000;80:209–19.
  • Jacobsen JN, Andersen AS, Sonnested MK, Laursen IA, Jorgensen B, Krogfelt KA. Investigating the humoral immune response in chronic venous leg ulcer patients colonised with Pseudomonas aeruginosa. Int Wound J 2011;8:33–43.
  • Andersen A, Hill KE, Stephens P, Thomas DW, Jorgensen B, Krogfelt KA. Bacterial profiling using skin grafting, standard culture and molecular bacteriological methods. J Wound Care 2007;16:171–5.
  • Fazli M, Bjarnsholt T, Kirketerp-Moller K, Jorgensen B, Andersen AS, Krogfelt K, . Non-random distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds. J Clin Microbiol 2009;47:4084–9.
  • Kirketerp-Moller K, Jensen PO, Fazli M, Madsen KG, Pedersen J, Moser C, . Distribution, organization, and ecology of bacteria in chronic wounds. J Clin Microbiol 2008;46:2717–22.
  • Mustoe TA, O'Shaughnessy K, Kloeters O. Chronic wound pathogenesis and current treatment strategies: a unifying hypothesis. Plast Reconstr Surg 2006;117:35S–41S.
  • Medina A, Scott PG, Ghahary A, Tredget EE. Pathophysiology of chronic nonhealing wounds. J Burn Care Rehabil 2005;26:306–19.
  • Grinnell F, Zhu M. Fibronectin degradation in chronic wounds depends on the relative levels of elastase, alpha1-proteinase inhibitor, and alpha2-macroglobulin. J Invest Dermatol 1996;106:335–41.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.