212
Views
5
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLE

Etiological diagnosis of bloodstream infections through a multiplex real-time polymerase chain reaction test in pediatric patients: a case series from a tertiary Italian hospital

, , , , , , , , , , & show all
Pages 73-79 | Received 05 Jun 2014, Accepted 16 Sep 2014, Published online: 26 Nov 2014

References

  • Goldstein B, Giroir B, Randolph A;International Consensus Conference on Pediatric Sepsis. International pediatric sepsis consensus conference: definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med 2005;6:2–8.
  • Lucignano B, Ranno S, Liesenfeld O, Pizzorno B, Putignani L, Bernaschi P, et al. Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol 2011;49:2252–8.
  • Mauro MV, Cavalcanti P, Perugini D, Noto A, Sperlì D, Giraldi C. Diagnostic utility of LightCycler SeptiFast and procalcitonin assays in the diagnosis of bloodstream infection in immunocompromised patients. Diagn Microbiol Infect Dis 2012;73:308–11.
  • Chang S-S, Hsieh W-H, Liu T-S, Lee SH, Wang CH, Chou HC, et al. Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis – a systemic review and meta-analysis. PloS One 2013;8:e62323.
  • De Pauw B, Walsh TJ, Donnelly JP, Stevens DA, Edwards JE, Calandra T, et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 2008;46:1813–21.
  • Nield LS, Kamat D. Fever without a focus. In: Kliegman RM, Stanton BF, St. Geme JW III, Schor NF, Behrman RE. Nelson Textbook of Pediatrics, 19th ed. Philadelphia: Elsevier Saunders. 2011. Chapter 170, pp. 896–901.
  • Freifeld AG, Bow EJ, Sepkowitz KA, Boeckh MJ, Ito JI, Mullen CA, et al. Clinical practice guideline for the use of antimicrobial agents in neutropenic patients with cancer: 2010 update by the Infectious Diseases Society of America. Clin Infect Dis 2011;52:e56–93.
  • Centers for Disease Control and Prevention (CDC). Examples of Clinical Definitions for Catheter-Related Infections. 2002. Available at: www.cdc.gov/mmwr/preview/mmwrhtml/rr5110a2.htm
  • Mermel LA, Allon M, Bouza E, Craven DE, Flynn P, O’Grady NP, et al. Clinical practice guidelines for the diagnosis and management of intravascular catheter-related infection: 2009 Update by the Infectious Diseases Society of America. Clin Infect Dis 2009;49:1–45.
  • Lamoth F, Jaton K, Prod'hom G, Senn L, Bille J, Calandra T, et al. Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 2010;48:3510–16.
  • Bell L, Hattangadi S. Microbiology and infectious diseases. In: Gunn VL, Nechyba C, editors. The Harriet Lane Handbook, 16th edn. Mosby. 2002. p 343.
  • Varani S, Stanzani M, Paolucci M, Melchionda F, Castellani G, Nardi L, et al. Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR. J Infect 2009;58:346–51.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.