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Original Article

Detection of Bacteremia by Buffy Coat Smears

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Pages 215-217 | Published online: 08 Jul 2009

References

  • Humphrey A A. Use of the buffy layer in the rapid diagnosis of septicemia. Am J Clin Pathol 1944; 14: 358–362
  • Brooks G E, Pribble A H, Beaty H N. Early diagnosis of bacteremia by buffy coat examination. Arch Intern Med 1973; 132: 673–675
  • Mirret S, Lauer B A, Miller G A, Reller L B. Comparison of acridine orange, methylene blue, and Gram stains for blood cultures. J Clin Microbiol 1982; 15: 562–566
  • Kronvall G, Myhre E. Differential staining of bacteria in clinical specimens using acridine orange buffered at low pH. Acta Pathol Microbiol Scand (B) 1977; 85: 249–250
  • Tierney B M, Henry N K, Washington J A, II. Early detection of positive blood cultures by the acridine orange staining technique. J Clin Microbiol 1983; 18: 830–833
  • Reik H, Rubin S J. Evaluation of the buffy coat smear for rapid detection of bacteremia. JAMA 1981; 245: 357–359
  • Greenfield M D, Clausen C R, Smith A L. The use of acridine orange as a rapid method for the quantitation of bacteremia in laboratory animals. Infection 1985; 13: 137–139

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