Precision cut lung slices as an efficient tool for in vitro lung physio-pharmacotoxicology studies

References

• Abdull Razis AF, Bagatta M, De Nicola GR, Iori R, Ioannides C. (2011). Up-regulation of cytochrome P450 and phase II enzyme systems in rat precision-cut rat lung slices by the intact glucosinolates, glucoraphanin and glucoerucin. Lung Cancer 71:298–305.
   PubMedGoogle Scholar
• Bach PH, Vickers AEM, Fisher R, Baumann A, Brittebo E, Carlile DJ, Kosher HJ, Lake BG, Salmon F, Sawyer TW, Skibinski G. (1996). The use of tissue slices for pharmacotoxicology studies – the report and recommendations of ECVAM workshop 20. ATLA Altern Lab Anim 24:893–923.
   Web of Science ®Google Scholar
• Baker RR. (1999). Smoke chemistry. In: Davis DL, Nielsen MT, eds. Tobacco Production, Chemistry and Technology. Oxford: Blackwell Science Ltd., 398–439.
   Google Scholar
• Balls M, Goldberg AM, Fentem JH, Broadhead CL, Burch RL, Festing MF, Frazier JM, Hendriksen CF, Jennings M, van der Kamp MD, Morton DB, Rowan AN, Russell C, Russell WM, Spielmann H, Stephens ML, Stokes WS, Straughan DW, Yager JD, Zurlo J, van Zutphen BF. (1995). The three Rs: the way forward: the report and recommendations of ECVAM Workshop 11. Altern Lab Anim 23:838–866.
   PubMed Web of Science ®Google Scholar
• Bergner A, Kellner J, Kemp da Silva A, Fischer R, Gamarra F, Huber RM. (2006). Bronchial hyperreactivity is correlated with increased baseline airway tone. Eur J Med Res 11:77–84.
   PubMedGoogle Scholar
• Bergner A, Sanderson MJ. (2002). Acetylcholine-induced calcium signaling and contraction of airway smooth muscle cells in lung slices. J Gen Physiol 119:187–198.
   PubMed Web of Science ®Google Scholar
• Bion A, Fal M, Gouriou F, Le Prieur E, Dionnet F, Morin JP. (2002). Biphasic culture of rat lung slices for pharmacotoxicological evaluation of complex atmospheres. Cell Biol Toxicol 18:301–314.
   PubMed Web of Science ®Google Scholar
• Churg A, Zhou S, Wang X, Wang R, Wright JL. (2009). The role of interleukin-1beta in murine cigarette smoke-induced emphysema and small airway remodeling. Am J Respir Cell Mol Biol 40:482–490.
   PubMed Web of Science ®Google Scholar
• Cooper PR, McParland BE, Mitchell HW, Noble PB, Politi AZ, Ressmeyer AR, West AR. (2009). Airway mechanics and methods used to visualize smooth muscle dynamics in vitro. Pulm Pharmacol Ther 22:398–406.
   PubMedGoogle Scholar
• Dandurand RJ, Wang CG, Phillips NC, Eidelman DH. (1993). Responsiveness of individual airways to methacholine in adult rat lung explants. J Appl Physiol 75:364–372.
   PubMedGoogle Scholar
• Dassow C, Wiechert L, Martin C, Schumann S, Müller-Newen G, Pack O, Guttmann J, Wall WA, Uhlig S. (2010). Biaxial distension of precision-cut lung slices. J Appl Physiol 108:713–721.
   PubMedGoogle Scholar
• Davis JM. (1967). The structure of guinea-pig lung maintained in organ culture. Br J Exp Pathol 48:371–378.
   PubMed Web of Science ®Google Scholar
• De Kanter R, De Jager MH, Draaisma AL, Jurva JU, Olinga P, Meijer DK, Groothuis GM. (2002). Drug-metabolizing activity of human and rat liver, lung, kidney and intestine slices. Xenobiotica 32:349–362.
   PubMed Web of Science ®Google Scholar
• De Kanter R, Monshouver M, Draaisma AL, De Jager MH, de Graaf IA, Proost JH, Meijer DK, Groothuis GM. (2004). Prediction of whole-body metabolic clearance of drugsthrough the combined use of slices from rat liver, lung, kidney, small intestine and colon. Xenobiotica 34:229–241.
   PubMed Web of Science ®Google Scholar
• Delmotte P Sanderson MJ. (2006). Ciliary beat is maintained at maximal rate in the small airways of lung slices. Am J Respir Cell Mol Biol 35:110–117.
   PubMedGoogle Scholar
• Fall M, Bion A, Keravec V, Ciss M, Diouf A, Dionnet F, Morin JP. (2008). Toxicological study of emissions resulting from a diesel and a gazoline engine using an organotypic culture of lung slice. Dakar Med 53:52–60.
   PubMedGoogle Scholar
• Fall M, Guerbet M, Park B, Gouriou F, Dionnet F, Morin JP. (2007). Evaluation of cerium oxide and cerium oxide based fuel additive safety on organotypic cultures of lung slices. Nanotoxicol 1:227–234.
   Web of Science ®Google Scholar
• Fisher RL, Shaughnessy RP, Jenkins PM, Austin ML, Roth GL, Gandolfi AJ, Brendel K. (1995). Dynamic organ-culture is superior to multiwell plate culture for maintaining precision-cut tissue-slices – optimization of tissue slice culture. Toxicol Methods 5:99–113.
   Web of Science ®Google Scholar
• Fisher RL, Smith MS, Hasal SJ, Hasal KS, Gandolfi AJ, Brendel K. (1994). The use of human lung slices in toxicology. Hum Exp Toxicol 13:466–471.
   PubMed Web of Science ®Google Scholar
• Freeman BA, Crapo JD. (1981). Hyperoxia increases oxygen radical production in rat lungs and lung mitochondria. J Biol Chem 256:10986–10992.
   PubMed Web of Science ®Google Scholar
• Georis B, Cardenas A, Buchet JP, Lauwerys R. (1990). Inorganic arsenic methylation by rat tissue slices. Toxicology 63:73–84.
   PubMed Web of Science ®Google Scholar
• Graaf IA, Groothuis GM, Olinga P. (2007). Precision-cut tissue slices as a tool to predict metabolism of novel drugs. Expert Opin Drug Metab Toxicol 3:879–898.
   PubMed Web of Science ®Google Scholar
• Guerrero RR, Rounds DE, Booher J. (1977). An improved organ culture method for adult mammalian lung. In Vitro 13:517–524.
   PubMedGoogle Scholar
• Hackney JD, Linn WS, Bell KA, Buckley RD. (1979). Health effects of air pollution: controlled studies in humans. In: Lee SD, Mudd JB, eds. Assessing Toxic Effects of Environmental Pollutants. Ann Arbor, MI: Ann Arbor Science Publishers Inc., 42–67.
   Google Scholar
• Hanlon N, Coldham N, Sauer MJ, Ioannides C. (2009). Modulation of rat pulmonary carcinogen-metabolising enzyme systems by the isothiocyanates erucin and sulforaphane. Chem Biol Interact 177:115–120.
   PubMedGoogle Scholar
• Harrigan JA, McGarrigle BP, Sutter TR, Olson JR. (2006). Tissue specific induction of cytochrome P450 (CYP) 1A1 and 1B1 in rat liver and lung following in vitro (tissue slice) and in vivo exposure to benzo(a)pyrene. Toxicol In Vitro 20:426–438.
   PubMed Web of Science ®Google Scholar
• Harrigan JA, Vezina CM, McGarrigle BP, Ersing N, Box HC, Maccubbin AE, Olson JR. (2004). DNA adduct formation in precision-cut rat liver and lung slices exposed to benzo[a]pyrene. Toxicol Sci 77:307–314.
   PubMed Web of Science ®Google Scholar
• Harris DT, Sakiestewa D, Robledo RF, Witten M. (1997). Protection from JP-8 jet fuel induced immunotoxicity by administration of aerosolized substance P. Toxicol Ind Health 13:571–588.
   PubMed Web of Science ®Google Scholar
• Hart NA, Van Der Plaats A, Faber A, Leuvenink HG, Olinga P, Wiersema-Buist J, Verkerke GJ, Rakhorst G, Ploeg RJ. (2005). Oxygenation during hypothermic rat liver preservation:an in vitro slice study to demonstrate beneficial or toxic oxygenation effects. Liver Transplant 11:1403–1411.
   PubMedGoogle Scholar
• Hasson V, Morin JP, Preterre D, Keravec V, Farin D, Dionnet F, Bion-Robin A, Meyer M. (2009). Exhaust toxicological profiles from direct injection engine with and without particulate filter regeneration during NEDC cycling. SAE Technical paper 2009-PFL-1214.
   Google Scholar
• Hays AM, Lantz RC, Witten ML. (2003). Correlation between in vivo and in vitro pulmonary responses to jet propulsion fuel-8 using precision-cut lung slices and a dynamic organ culture system. Toxicol Pathol 31:200–207.
   PubMed Web of Science ®Google Scholar
• Held HD, Martin C, Uhlig S. (1999). Characterization of airway and vascular responses in murine lungs. Br J Pharmacol 126:1191–1199.
   PubMed Web of Science ®Google Scholar
• Kasper M, Seidel D, Knels L, Morishima N, Neisser A, Bramke S, Koslowski R. (2004). Early signs of lung fibrosis after in vitro treatment of rat lung slices with CdCl2 and TGF-beta1. Histochem Cell Biol 121:131–140.
   PubMed Web of Science ®Google Scholar
• Kaur-Knudsen D, Nordestgaard BG, Tybjaerg-Hansen A, Bojesen SE. (2009). CYP1B1 genotype and risk of cardiovascular disease, pulmonary disease, and cancer in 50 000 individuals. Pharmacogenet Genom 19:685–694.
   PubMed Web of Science ®Google Scholar
• Kellner J, Gamarra F, Welsch U, Jörres RA, Huber RM, Bergner A. (2007). IL-13Ralpha2 reverses the effects of IL-13 and IL-4 on bronchial reactivity and acetylcholine-induced Ca+ signaling. Int Arch Allergy Immunol 142:199–210.
   PubMed Web of Science ®Google Scholar
• Kida H, Yoshida M, Hoshino S, Inoue K, Yano Y, Yanagita M, Kumagai T, Osaki T, Tachibana I, Saeki Y, Kawase I. (2005). Protective effect of IL-6 on alveolar epithelial cell death induced by hydrogen peroxide. Am J Physiol Lung Cell Mol Physiol 288:L342–L349.
   PubMed Web of Science ®Google Scholar
• Kinnard WV, Tuder R, Papst P, Fisher JH. (1994). Regulation of alveolar type II cell differentiation and proliferation in adult rat lung explants. Am J Respir Cell Mol Biol 11:416–425.
   PubMedGoogle Scholar
• Kurosawa H, Wang CG, Dandurand RJ, King M, Eidelman DH. (1995). Mucociliary function in the mouse measured in explanted lung tissue. J Appl Physiol 79:41–46.
   PubMedGoogle Scholar
• Lake BG, Meredith C, Scott MP, Renwick AB, Price RJ. (2003). Use of cultured precision-cut rat lung slices to study the in vitro induction of pulmonary cytochrome P450 forms. Xenobiotica 33:691–702.
   PubMed Web of Science ®Google Scholar
• Langford AM, Hobbs MJ, Upshall DG, Blain PG, Williams FM. (1996). The effect of sulphur mustard on glutathione levels in rat lung slices and the influence of treatment with arylthiols and cysteine esters. Hum Exp Toxicol 15:619–624.
   PubMed Web of Science ®Google Scholar
• Le Prieur E, Vaz E, Bion A, Dionnet F, Morin JP. (2000). Toxicity of diesel engine exhausts in an in vitro model of lung slices in biphasic organotypic culture: induction of a proinflammatory and apoptotic response. Arch Toxicol 74:460–466.
   PubMed Web of Science ®Google Scholar
• Liberati TA, Randle MR, Toth LA. (2010). In vitro lung slices: a powerful approach for assessment of lung pathophysiology. Expert Rev Mol Diagn 10:501–508.
   PubMed Web of Science ®Google Scholar
• Lin JC, Roy JP, Verreault J, Talbot S, Côté F, Couture R, Morin A. (2012). An ex vivo approach to the differential parenchymal responses induced by cigarette whole smoke and its vapor phase. Toxicology 293:125–131.
   PubMedGoogle Scholar
• Lin CJ, Yang PC, Hsu MT, Yew FH, Liu TY, Shun CT, Tyan SW, Lee TC. (1998). Induction of pulmonary fibrosis in organ-cultured rat lung by cadmium chloride and transforming growth factor-beta1. Toxicology 127:157–166.
   PubMed Web of Science ®Google Scholar
• Madhu C, Rix P, Small D, Yuan YD, Tang-Liu DD. (1997). The use of precision-cut rat lung slices for studying PGF(2a) metabolism. Environ Toxicol Pharmacol 3:251–256.
   PubMed Web of Science ®Google Scholar
• Martin C, Uhlig S, Ullrich V. (2001). Cytokine-induced bronchoconstriction in precision-cut lung slices is dependent upon cyclooxygenase-2 and thromboxane receptor activation. Am J Respir Cell Mol Biol 24:139–145.
   PubMed Web of Science ®Google Scholar
• McBride S, Rannie D, Harrison DJ. (2000). Gene transfer to adult human lung tissue ex vivo. Gene Ther 7:675–678.
   PubMedGoogle Scholar
• Monteil C, Guerbet M, Le Prieur E, Morin JP, Jouany JM, P Fillastre J. (1999a). Characterization of precision-cut rat lung slices in a biphasic gas/liquid exposure system: Effect of O2. Toxicol In Vitro 13:467–473.
   PubMedGoogle Scholar
• Monteil C, Le Prieur E, Buisson S, Morin JP, Guerbet M, Jouany JM. (1999b). Acrolein toxicity: comparative in vitro study with lung slices and pneumocytes type II cell line from rats. Toxicology 133:129–138.
   PubMed Web of Science ®Google Scholar
• Moreno L, Perez-Vizcaino F, Harrington L, Faro R, Sturton G, Barnes PJ, Mitchell JA. (2006). Pharmacology of airways and vessels in lung slices in situ: role of endogenous dilator hormones. Respir Res 7:111.
   PubMedGoogle Scholar
• Morin JP, Fouquet F, Monteil C, Le Prieur E, Vaz E, Dionnet F. (1999). Development of a new in vitro system for continuous in vitro exposure of lung tissue to complex atmospheres: application to diesel exhaust toxicology. Cell Biol Toxicol 15:143–152.
   PubMed Web of Science ®Google Scholar
• Morin JP, Gouriou F, Preterre D, Bobbia M, Delmas V.(2009). Evaluation de l’exposition aux polluantsatmosphériques des conducteurs de véhicules automobiles par la mise en œuvre de mesures dynamiques dans l’habitacle du véhicule. Archives des Maladies Professionnelles et de l’Environnement 70:184–192.
   Google Scholar
• Morin JP, Hasson V, Fall M, Papaioanou E, Preterre D, Gouriou F, Keravec V, Konstandopoulos A, Dionnet F. (2008). Prevalidation of in vitro continuous flow exposure systems as alternatives to in vivo inhalation safety evaluation experimentations: outcome from MAAPHRI-PCRD5 research program. Exp Toxicol Pathol 60:195–205.
   PubMed Web of Science ®Google Scholar
• Morin JP, Keravec V, Preterre D, Dionnet F. (2011). Toxic impacts of emissions from small 50cc engine run under EC47 driving cycle: a comparison between 2-stroke and 4-stroke engines and lube oil quality and ethanol additivation SAE Technical paper: 2011-11ICE-0143.
   Google Scholar
• Morin JP, Rumigny JF, Bion A, Dionnet F. (2002). Isoflavones protect against diesel engine exhaust injury in organotypic culture of lung tissue. Environ Toxicol Pharmacol 12:213–220.
   PubMed Web of Science ®Google Scholar
• Mustafa MG, DeLucia AJ, York GK, Arth C, Cross CE. (1973). Ozone interaction with rodent lung. II. Effects on oxygen consumption of mitochondria. J Lab Clin Med 82:357–365.
   PubMed Web of Science ®Google Scholar
• Mustafa MG, Cross CE. (1974). Effects of short-term ozone exposure on lung mitochondrial oxidative and energy metabolism. Arch Biochem Biophys 162:585–594.
   PubMed Web of Science ®Google Scholar
• Mustafa MG, Hacker AD, Ospital JJ, Hussain MZ, Lee SD. (1977). Biochemical effects ofenvironmental oxidant pollutants in animal lungs. In: Lee SD, ed. Biochemical Effects of Environmental Pollutants. Ann Arbor, MI: Ann Arbor Science Publishers, Inc., 59.
   Google Scholar
• Olinga P, Groen K, Hof IH, De Kanter R, Koster HJ, Leeman WR, Rutten AA, Van Twillert K, Groothuis GM. (1997). Comparison of five incubation systems for rat liver slices using functional and viability parameters. J Pharmacol Toxicol Methods 38:59–69.
   PubMed Web of Science ®Google Scholar
• Olinga P, Merema M, Hof IH, de Jong KP, Slooff MJ, Meijer DK, Groothuis GM. (1998). Effect of human liver source on the functionality of isolated hepatocytes andliver slices. Drug Metab Dispos 6:5–11.
   Google Scholar
• Perez-Zoghbi JF, Bai Y, Sanderson MJ. (2010). Nitric oxide induces airway smooth muscle cell relaxation by decreasing the frequency of agonist-induced Ca2+ oscillations. J Gen Physiol 135:247–259.
   PubMed Web of Science ®Google Scholar
• Parrish AR, Gandolfi AJ, Brendel K. (1995). Precision-cut tissue slices: applications in pharmacology and toxicology. Life Sci 57:1887–1901.
   PubMed Web of Science ®Google Scholar
• Price RJ, Ball SE, Renwick AB, Barton PT, Beamand JA, Lake BG. (1998). Use of precision-cut rat liver slices for studies of xenobiotic metabolism and toxicity: comparison of the Krumdieck and Brendel tissue slicers. Xenobiotica 28:361–371.
   PubMed Web of Science ®Google Scholar
• Price RJ, Renwick AB, Beamand JA, Esclangon F, Wield PT, Walters DG, Lake BG. (1995). Comparison of the metabolism of 7-ethoxycoumarin and coumarin in precision-cut rat liver and lung slices. Food Chem Toxicol 33:233–237.
   PubMed Web of Science ®Google Scholar
• Price RJ, Walters DG, Hoff C, Mistry H, Renwick AB, Wield PT, Beamand JA, Lake BG. (1996). Metabolism of [ring-U-14C] agaritine by precision-cut rat, mouse and human liver and lung slices. Food Chem Toxicol 34:603–609.
   PubMed Web of Science ®Google Scholar
• Punyadarsaniya D, Liang CH, Winter C, Petersen H, Rautenschlein S, Hennig-Pauka I, Schwegmann-Wessels C, Wu CY, Wong CH, Herrler G. (2011). Infection of differentiated porcine airway epithelial cells by influenza virus: differential susceptibility to infection by porcine and avian viruses. PLoS ONE 6:e28429.
   PubMed Web of Science ®Google Scholar
• Pushparajah DS, Umachandran M, Plant KE, Plant N, Ioannides C. (2007). Evaluation of the precision-cut liver and lung slice systems for the study of induction of CYP1, epoxide hydrolase and glutathione S-transferase activities. Toxicology 231:68–80.
   PubMed Web of Science ®Google Scholar
• Rausch SM, Martin C, Bornemann PB, Uhlig S, Wall WA. (2011). Material model of lung parenchyma based on living precision-cut lung slice testing. J Mech Behav Biomed Mater 4:583–592.
   PubMedGoogle Scholar
• Ressmeyer AR, Larsson AK, Vollmer E, Dahlèn SE, Uhlig S, Martin C. (2006). Characterisation of guinea pig precision-cut lung slices: comparison with human tissues. Eur Respir J 28:603–611.
   PubMed Web of Science ®Google Scholar
• Rieg AD, Rossaint R, Uhlig S, Martin C. (2011). Cardiovascular agents affect the tone of pulmonary arteries and veins in precision-cut lung slices. PLoS ONE 6:e29698.
   PubMed Web of Science ®Google Scholar
• Russel WMS, Burch RL. (1959). The Principles of Humane Experimental Technique, Methuen, London, UK. Facsimile edition (1992). Potters Bar, Herts, UK: UFAW, 238.
   Google Scholar
• Saunders NA, McGeachie JK, Ilett KF, Minchin RF. (1989). Localization of spermidine uptake in rabbit lung slices. Am J Physiol 257:C579–C587.
   Google Scholar
• Saunders NA, Rigby PJ, Ilett KF, Minchin RF. (1988). Autoradiographic localization of putrescine uptake to type II pneumocytes of rabbit lung slices. Lab Invest 59:380–386.
   PubMedGoogle Scholar
• Sawyer TW, Wilde PE, Rice P, Weiss MT. (1995). Toxicity of sulphur mustard in adult rat lung organ culture. Toxicology 100:39–49.
   PubMedGoogle Scholar
• Schlepütz M, Uhlig S, Martin C. (2011). Electric field stimulation of precision-cut lung slices. J Appl Physiol 110:545–554.
   PubMedGoogle Scholar
• Shapiro PS, Casty FE, Stirewalt WS, Leslie KO, Absher MP, Evans JN. (1994). Oxygen-induced changes in protein synthesis and cell proliferation in cultured lung slices. Am J Physiol 267:L720–L727.
   Google Scholar
• Shi W, Eidelman DH, Michel RP. (1997). Differential relaxant responses of pulmonary arteries and veins in lung explants of guinea pigs. J Appl Physiol 83:1476–1481.
   PubMedGoogle Scholar
• Siminski JT, Kavanagh TJ, Chi E, Raghu G. (1992). Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions. Am J Physiol 262:L105–L110.
   PubMed Web of Science ®Google Scholar
• Springer J, Fischer A. (2003). Substance P-induced pulmonary vascular remodelling in precision cut lung slices. Eur Respir J 22:596–601.
   PubMedGoogle Scholar
• Streck E, Jörres RA, Huber RM, Bergner A. (2010). Effects of cigarette smoke extract and nicotine on bronchial tone and acetylcholine-induced airway contraction in mouse lung slices. J Investig Allergol Clin Immunol 20:324–330.
   PubMedGoogle Scholar
• Switalla S, Knebel J, Ritter D, Krug N, Braun A, Sewald K. (2010). Effects of acute in vitro exposure of murine precision-cut lung slices to gaseous nitrogen dioxide and ozone in an air-liquid interface (ALI) culture. Toxicol Lett 196:117–124.
   PubMedGoogle Scholar
• Trowell OA. (1959). The culture of mature organs in a synthetic medium. Exp Cell Res 16:118–147.
   PubMed Web of Science ®Google Scholar
• Umachandran M, Howarth J, Ioannides C. (2004). Metabolic and structural viability of precision-cut rat lung slices in culture. Xenobiotica 34:771–780.
   PubMed Web of Science ®Google Scholar
• Umachandran M, Ioannides C. (2006). Stability of cytochromes P450 and phase II conjugation systems in precision-cut rat lung slices cultured up to 72 h. Toxicology 224:14–21.
   PubMed Web of Science ®Google Scholar
• Vallan C, Friis RR, Burri PH. (1995). Release of a mitogenic factor by adult rat lung slices in culture. Exp Lung Res 21:469–487.
   PubMed Web of Science ®Google Scholar
• Vickers AE, Jimenez RM, Spaans MC, Pflimlin V, Fisher RL, Brendel K. (1997). Human and rat lung biotransformation of cyclosporin A and its derivatives using slices and bronchial epithelial cells. Drug Metab Dispos 25:873–880.
   PubMed Web of Science ®Google Scholar
• Vietmeier J, Niedorf F, Bäumer W, Martin C, Deegen E, Ohnesorge B, Kietzmann M. (2007). Reactivity of equine airways–a study on precision-cut lung slices. Vet Res Commun 31:611–619.
   PubMed Web of Science ®Google Scholar
• Wang I, Politi AZ, Tania N, Bai Y, Sanderson MJ, Sneyd J. (2008). A mathematical model of airway and pulmonary arteriole smooth muscle. Biophys J 94:2053–2064.
   PubMed Web of Science ®Google Scholar
• Warren JS, Barton PA. (1992). In vitro analysis of pulmonary inflammation using rat lung organ cultures. Exp Lung Res 18:55–67.
   PubMed Web of Science ®Google Scholar
• Weinhold PA, Burkel WE, Fischer TV, Kahn RH. (1979). Adult rat lung in organ culture: maintenance of histotypic structure and ability to synthesize phospholipid. In Vitro 15:1023–1031.
   PubMedGoogle Scholar
• Wohlsen A, Martin C, Vollmer E, Branscheid D, Magnussen H, Becker WM, Lepp U, Uhlig S. (2003). The early allergic response in small airways of human precision-cut lung slices. Eur Respir J 21:1024–1032.
   PubMed Web of Science ®Google Scholar
• Williams MC. (1977). Conversion of lamellar body membranes into tubular myelin in alveoli of fetal rat lungs. J Cell Biol 72:260–277.
   PubMed Web of Science ®Google Scholar
• Wiseman H. (2000). The therapeutic potential of phytoestrogens. Expert Opin Investig Drugs 9:1829–1840.
   PubMed Web of Science ®Google Scholar
• Wright JL, Churg A. (2008). Short-term exposure to cigarette smoke induces endothelial dysfunction in small intrapulmonary arteries: analysis using guinea pig precision cut lung slices. J Appl Physiol 104:1462–1469.
   PubMed Web of Science ®Google Scholar