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Xenobiotica
the fate of foreign compounds in biological systems
Volume 22, 1992 - Issue 8
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Research Article

Hep G2 cell line as a human model for sulphate conjugation of drugs

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Pages 973-982 | Received 01 May 1991, Accepted 01 Feb 1992, Published online: 22 Sep 2008

References

  • Anderson R. J., Weinshilboum R. M. Phenolsulphotransferase in human tissue: radiochemical enzymatic assay and biochemical properties. Clinica Chimica Acta 1980; 103: 79–90
  • Baranczyk-Kuzma A., Garren J. A., Hidalgo I. J., Borchardt R. T. Substrate specificity and some properties of phenol sulfotransferase from intestinal Caco-2 cells. Life Sciences 1991; 49: 1197–1206
  • Campbell N. R. C., Van Loon J. A., Weinshilboum R. M. Human liver phenolsulfo-transferase: assay conditions, biochemical properties and partial purification of isoenzymes of the thermostable form. Biochemical Pharmacology 1987a; 36: 1435–1446
  • Campbell N. R. C., Van Loon J. A., Sundaham R. S., Ames M. M., Hansch C., Weinshilboum R. Human and rat liver phenol sulfotransferase: structure-activity relationships for phenolic substrates. Molecular Pharmacology 1987b; 32: 813–819
  • Dawson J. R., Adams D. J., Wolf C. R. Induction of drug metabolizing enzymes in human liver cell line Hep G2. FEBS Letters 1985; 183: 219–222
  • Doostdar H., Demoz A., Burke M. D., Melvin W. T., Grant M. H. Variation in drug-metabolizing enzyme activities during the growth of human Hep G2 hepatoma cells. Xenobiotica 1990; 20: 435–441
  • Falany C. N. Molecular enzymology of human liver cytosolic sulfotransferases. Trends in Pharmacological Sciences 1991; 12: 255–259
  • Fischer V., Wiebel F. J. Metabolism of fluperlapine by cytochrome P450-dependent and flavin-dependent monooxygenases in continuous cultures of rat and human cells. Biochemical Pharmacology 1990; 39: 1327–1333
  • Foldes A., Meek J. L. Rat brain phenolsulfotransferase—partial purification and some properties. Biochimica Biophysica Acta 1973; 327: 365–374
  • Grant M. H., Burke M. D., Hawksworth G. M., Duthie S. J., Engeset J., Petrie J. C. Human adult hepatocytes in primary monolayer culture—maintenance of mixed function oxidase and conjugation pathways of drug metabolism. Biochemical Pharmacology 1987; 36: 2311–2316
  • Grant H. M., Duthie S. J., Gray A. G., Burke M. D. Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line. Biochemical Pharmacology 1988; 37: 4111–4116
  • Grant M. H., Hawksworth G. M. The activity of UDP-glucuronyltransferase, sulfo-transferase and glutathione-S-transferase in primary cultures of rat hepatocytes. Biochemical Pharmacology 1986; 35: 2979–2982
  • Kienzl E., Eichinger K. Preparation of dopamine 3-O-sulphate and dopamine 4-O-sulphate as reference substances and high-performance liquid chromatographic trace determination. Journal of Chromatography 1988; 430: 263–269
  • Liu M. C., Yu S., Suiko M. Tyramine-O-sulfate, in addition to tyrosine-O-sulfate, is produced and secreted by Hep G2 human hepatoma cells, but not by 3Y1 rat embryo fibroblasts. Biochemistry International. 1990; 21: 815–821
  • Lowry O. H., Rosebrough N. J., Farr A., Randall R. J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 1951; 193: 265–275
  • Mulder G. J. Sulfation of Drugs and Related Compounds. CRC Press, Boca Raton, FLUSA 1981
  • Mulder G. J., Jakoby W. B. Sulfation. Conjugation Reactions in Drug Metabolism: An Integrated Approach. Substrates, Co-substrates, Enzymes and Their Interactions In Vivo and In Vitro, G. J. Mulder. Taylor & Francis, London 1990; 107–161
  • Nimura N., Ogura H., Kinoshita T. Reversed-phase liquid chromatographic resolution of amino acid enantiomers by derivatization with 2,3,4,6-tetra-O-β-D-glucopyranosyl isothio-cyanate. Journal of Chromatography 1980; 202: 375–379
  • Oatis J. E., Jr., Russell M. P., Knapp D. R., Walle T. Ring-hydroxylated propranolol: synthesis and β-receptor antagonist and vasodilating activities of the seven isomers. Journal of Medicinal Chemistry 1981; 24: 309–314
  • Oatis J. E., Jr., Walle T., Daniell H. B., Gaffney T. E., Knapp D. R. Synthesis of 4′-hydroxypropranolol sulfate, a major non-β-blocking propranolol metabolite in man. Journal of Medicinal Chemistry 1985; 28: 822–824
  • Patel D. K., Shockcor J. P., Chang S. Y., Sigel C. W., Huber B. E. Metabolism of a novel antitumor agent, crisnatol, by a human hepatoma cell line, Hep G2, and hepatic microsomes. Biochemical Pharmacology 1991; 42: 337–346
  • Rein G., Glover V., Sandler M. Multiple forms of phenolsulphotransferase in human tissues. Selective inhibition by dichloronitrophenol. Biochemical Pharmacology 1982; 31: 1893–1897
  • Reiter C., Mwaluko G., Dunnette J., Van Loon J., Weinshilboum R. Thermolabile and thermostable human platelet phenol sulfotransferase. Substrate specificity and physical separation. Naunyn-Schmiedeberg's Archives of Pharmacology 1983; 324: 140–147
  • Sassa S., Sugita O., Galbraith R. A., Kappas A. Drug metabolism by the human hepatoma cell, Hep G2. Biochemical and Biophysical Research Communications 1987; 143: 52–57
  • Steiner A., Walle U. K., Walle T. Induction of propranolol metabolism in the Hep G2 human hepatoma cell line. Journal of Pharmacy and Pharmacology 1992, accepted for publication
  • Sundaram R. S., Szumlanski C., Otterness D., Van Loon J. A., Weinshilboum R. M. Human intestinal phenol sulfotransferase: assay conditions, activity levels and partial purification of the thermolabile form. Drug Metabolism and Disposition 1989; 17: 255–264
  • Walle T., Walle U. K. Stereoselective sulphate conjugation of racemic terbutaline by human liver cytosol. British Journal of Clinical Pharmacology 1990; 30: 127–133
  • Walle T., Walle U. K. Stereoselective sulfate conjugation of racemic 4-hydroxypropranolol by human and rat liver cytosol. Drug Metabolism and Disposition 1991a; 19: 448–453
  • Walle T., Walle U. K. Stereoselective sulfate conjugation of 4-hydroxypropranolol and terbutaline by the human liver phenolsulfotransferases. Drug Metabolism and Disposition 1992; 20: 333–336
  • Walle U. K., Walle T. Stereoselective sulfate conjugation of 4-hydroxypropranolol in humans: comparison of platelet and hepatic activity. Drug Metabolism and Disposition 1991b; 19: 838–840
  • Weinshilboum R. M. Phenol sulfotransferase in humans: properties, regulation, and function. Federation Proceedings 1986; 45: 2223–2228
  • Whittemore R. M., Pearce L. B., Roth J. A. Purification and kinetic characterization of a dopamine-sulfating form of phenol sulfotransferase from human brain. Biochemistry 1985; 24: 2477–2482
  • Young W. F., Okazaki H., Laws E. R., Jr., Weinshilboum R. M. Human brain phenol sulfotransferase: biochemical properties and regional localization. Journal of Neurochemistry 1984; 43: 706–715

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