Publication Cover
Xenobiotica
the fate of foreign compounds in biological systems
Volume 24, 1994 - Issue 2
93
Views
74
CrossRef citations to date
0
Altmetric
Original Article

Collagen gel immobilization: A useful cell culture technique for long-term metabolic studies on human hepatocytes

, , &
Pages 95-107 | Received 30 Nov 1993, Published online: 27 Aug 2009

References

  • Acosta D., Anuforo D. C., McMillin R., Soine W. H., Smith R. V. Comparison of cytochrome P-450 levels in adult rat liver, postnatal rat liver, and primary cultures of postnatal rat hepatocytes. Life Sciences 1979; 25: 1413–1418
  • Begue J. M., Guguen-Guillouzo C., Pasdeloup N., Guillouzo A. Prolonged maintenance of active cytochrome P-450 in adult rat hepatocytes co-cultured with another liver cell type. Hepatology 1984; 4: 839–842
  • Bissell D. M., Arenson D. M., Maher J. J., Roll F. J. Support of cultured hepatocytes by a laminin-rich gel. Journal of Clinical Investigation 1987; 79: 801–812
  • Blankson E. A., Chenery R. J., Paine A. J. Cytochrome P450 maintenance and diazepam metabolism in cultured rat hepatocytes. Biochemical Pharmacology 1991; 42: 1241–1245
  • Bolcsfoldi G., Johansson M., Andersson B., Moldeus P. Biotransformation of xenobiotics in isolated dog hepatocytes. Acta Pharmacologica et Toxicologica 1981; 48: 227–232
  • Chenery R. J., Ayrton A., Oldham H. G., Standring P., Norman S. J., Seddon T., Kirby R. Diazepam metabolism in cultured hepatocytes from rat, rabbit, dog, guinea pig, and man. Drug Metabolism and Disposition 1987; 15: 312–317
  • Dich J., Vind C., Grunnet N. Long-term culture of hepatocytes: effects of hormones on enzyme activities and metabolic capacity. Hepatology 1988; 8: 39–45
  • Dunn J. C. Y., Tompkins R. G., Yarmush M. L. Hepatocytes in collagen sandwich: evidence for transcriptional and translational regulation. Journal of Cell Biology 1992; 116: 1043–1053
  • Dunn J. C. Y., Yarmush M. L., Koebe H. G., Tompkins R. G. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB Journal 1989; 3: 174–177
  • Eckert K. G., Eyer P., Sonnenbichler J., Zetl I. Activation and detoxication of aminophenols. II. Synthesis and structural elucidation of various thiol addition products of 1,4-benzoquinoneimine and N-acetyl-1,4-benzoquinoneimine. Xenobiotica 1990; 20: 333–350
  • Elsdale T., Bard J. Collagen substrata for studies on cell behavior. Journal of Cell Biology 1972; 54: 626–637
  • Eyer P., Kampffmeyer H. G. Biotransformation of 4-dimethylaminophenol in the isolated perfused rat liver and in the rat. Biochemical Pharmacology 1978; 27: 2223–2228
  • Gómez-Lechón M. J., Montoya A., López P., Donato T., Larrauri A., Castell J. V. The potential use of cultured hepatocytes in predicting the hepatotoxicity of xenobiotics. Xenobiotica 1988; 18: 725–735
  • Grant M. H., Burke M. D., Hawksworth G. M., Duthie S. J., Engeset J., Petrie J. C. Human adult hepatocytes in primary monolayer culture. Biochemical Pharmacology 1987; 36: 2311–2316
  • Grant M. H., Duthie S. J., Gray A. G., Burke M. D. Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line. Biochemical Pharmacology 1988; 37: 4111–4116
  • Grant M. H., Hawksworth G. M. The activity of UDP-glucuronyltransferase, sulphotransferase and glutathione S-transferase in primary cultures of rat hepatocytes. Biochemical Pharmacology 1986; 35: 2979–2982
  • Guguen-Guillouzo C., Clement B., Baffet G., Beaumont C., Morel-Chany E., Glaise D., Guillouzo A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Experimental Cell Research 1983; 143: 47–54
  • Guguen-Guillouzo C., Gripon P., Vandenberghe Y., Lambelle F., Ratanasavanh D., Guillouzo A. Hepatotoxicity and molecular aspects of hepatocyte function in primary culture. Xenobiotica 1988; 18: 773–783
  • Guillouzo A., Beaune P., Gascoin M. N., Begue J. M., Campion J. P., Guengerich F. P., Guguen-Guillouzo C. Maintenance of cytochrome P-450 in cultured adult human hepatocytes. Biochemical Pharmacology 1985; 34: 2991–2995
  • Guzelian P. S., Li D., Schuetz E. G., Thomas P., Levin W., Mode A., Gustafsson J. A. Sex change in cytochrome P-450 phenotype by growth hormone treatment of adult rat heopatocytes maintained in a culture system on matrigel. Proceedings of the National Academy of Sciences, USA 1988; 85: 9783–9787
  • Hammond A. H., Fry J. R. Effect of serum-free medium on cytochrome P450-dependent metabolism and toxicity in rat cultured hepatocytes. Biochemical Pharmacology 1992; 44: 1461–1464
  • Hansch C., Leo A. Substituent Constants for Correlation Analysis in Chemistry and Biology. Wiley, New York 1979
  • Isom H. C., Secott T., Geogoff I., Woodworth C., Mummaw J. Maintenance of differential rat hepatocytes in primary culture. Proceedings of the National Academy of Sciences, USA 1985; 82: 3252–3256
  • Koebe H. G., Dunn J. C. Y., Toner M., Sterling L., Yarmush M. L., Tompkins R. G. A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration. Cryobiology 1990; 11: 576–584
  • Lake B. G., Paine A. J. The effect of hepatocyte culture conditions on cytochrome P-450 linked drug metabolising enzymes. Biochemical Pharmacology 1982; 31: 2141–2144
  • Lindsay C. K., Chenery R. J., Hawksworth G. M. Primary culture of rat hepatocytes in the presence of dimethyl sulphoxide. Biochemical Pharmacology 1991; 42: 17–25
  • Miyazaki M., Handa Y., Oda M., Yabe T., Miyano K., Sato J. Long-term survival of functional hepatocytes from adult rat in the presence of phenobarbital in primary culture. Experimental Cell Research 1985; 159: 176–190
  • Muakkassah-Kelly S. F., Bieri F., Waechter F., Bentley P., Stäubli W. Long-term maintenance of hepatocytes in primary culture in the presence of DMSO: further characterization and effect of Nafenopin, a peroxisome proliferator. Experimental Cell Research 1987; 171: 37–51
  • Nelson K. F., Acosta D. Long-term maintenance and induction of cytochrome P-450 in primary cultures of rat hepatocytes. Biochemical Pharmacology 1982; 31: 2211–2214
  • Paine A. J., Hockin L. J. Nutrient imbalance causes the loss of cytochrome P-450 in liver cell culture: formulation of culture media which maintain cytochrome P-450 at in vivo concentrations. Biochemical Pharmacology 1980; 29: 3215–3218
  • Paine A. J., Hockin L. J., Allen C. M. Long term maintenance and induction of cytochrome P-450 in rat liver cell culture. Biochemical Pharmacology 1982; 31: 1175–1178
  • Pertoft H., Rubin K., Kjellén L., Laurent T. C., Klingeborn B. The viability of cells grown or centrifuged in a new density gradient medium, PercollTM. Experimental Cell Research 1977; 110: 449–457
  • Reese J. A., Byard J. L. Isolation and culture of adult hepatocytes from liver biopsies. In vitro 1981; 17: 935–940
  • Reid L. M., Jefferson D. M. Culturing hepatocytes and other differentiated cells. Hepatology 1984; 4: 548–559
  • Richard B., Fabre G., De Sousa G., Fabre I., Rahmani R., Cano J. P. Interspecies variability in mitoxantrone metabolism using primary cultures of hepatocytes isolated from rat, rabbit and humans. Biochemical Pharmacology 1991; 41: 255–262
  • Rogiers V., Vandenberghe Y., Callaerts A., Verleye G., Cornet M., Mertens K., Sonk W., Vercruysse A. Phase I and phase II xenobiotic biotransformation in cultures and co-cultures of adult rat hepatocytes. Biochemical Pharmacology 1990; 40: 1701–1706
  • Rojkind M., Gatmaitan Z., Mackensen S., Giambrone M. A., Ponce P., Reid L. M. Connective tissue biomatrix: its isolation and utilisation for long-term cultures of normal rat hepatocytes. Journal of Cell Biology 1980; 87: 255–263
  • Ryan C. M., Carter E. A., Jenkins R. L., Sterling L. M., Yarmush M. L., Malt R. A., Tompkins R. G. Isolation and long-term culture of human hepatocytes. Surgery 1993; 113: 48–54
  • Saad B., Schawalder H., Maier P. Crude liver membrane fractions as substrate preserve liver-specific functions in long-term, serum-free rat hepatocyte cultures. In vitro 1993; 29A: 32–40
  • Schuetz E. G., Li D., Omiecinski C. J., Muller-Eberhard U., Kleinman H. K., Elswick B., Guzelian P. S. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. Journal of Cellular Physiology 1988; 134: 309–323
  • Soucek P., Gut I. Cytochromes P450 in rats: structures, functions, properties and relevant human forms. Xenobiotica 1992; 22: 83–103
  • Strom S. C., Jirtle R. L., Jones R. S., Novicki D. L., Rosenberg M. R., Novotny A., Irons G., McLain J. R., Michalopoulos G. Isolation, culture, and transplantation of human hepatocytes. Journal of the National Cancer Institute 1982; 68: 771–778
  • Suolinna E. M., Pitkäranta T. Effect of culture age on drug metabolizing enzymes and their induction in primary cultures of rat hepatocytes. Biochemical Pharmacology 1986; 35: 2241–2245
  • Tee L. B. G., Seddon T., Boobis A. R., Davies D. S. Drug metabolising activity of freshly isolated human hepatocytes. British Journal of Clinical Pharmacology 1985; 19: 279–294
  • Tong J. Z., De Lagausie P., Furlan V., Cresteil T., Bernard O., Alvarez F. Long-term culture of adult rat hepatocyte spheroids. Experimental Cell Research 1992; 200: 326–332
  • Vind C., Dich J., Grunnet N. The content and activity of cytochrome P450 in long-term culture of hepatocytes from male and female rats. Biochemical Pharmacology 1988; 37: 1371–1375
  • Wright M. C., Paine A. J. Evidence that the loss of rat liver cytochrome P450 in vitro is not solely associated with the use of collagenase, the loss of cell-cell contacts and/or the absence of an extracellular matrix. Biochemical Pharmacology 1992; 43: 237–243

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.