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Research Article

Cytotoxic effects of etephon and maleic hydrazide in Vero, Hep2, HepG2 cells

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Pages 459-465 | Received 19 Sep 2013, Accepted 13 Jan 2014, Published online: 04 Feb 2014

References

  • Almeida-Silva M, Caputo MM. (2012). Ripening and the use of ripeners for better sugarcane management. In: Marin FR, ed. Crop management – cases and tools for higher yield and sustainability. Rijeka, Croatia: InTech Publishing, 4
  • Butler M. (2004). Animal cell culture and technology. New York: Taylor & Francis, 32–33
  • Caprita A, Caprita R. (2005). Plant growth retardants for the treatment of vegetables used as raw materials for the food. J Agroaliment Process Technol 11:173–178
  • Cetinkaya MA, Baydan E. (2010). Investigation of in vitro effects of ethephon and chlorpyrifos, either alone or in combination, on rat intestinal muscle contraction. Interdiscip Toxicol 3:35–39
  • DTU (Technical University of Denmark). (2012). External Scientific Report submitted to EFSA. Available from: http://www.efsa.europa.eu/de/search/doc/269e.pdf. Accessed on November 11, 2013
  • EFSA (European Food Safety Authority). (2008). Scientific Report – Conclusion regarding the peer review of the pesticide risk assessment of the active substance ethephon. EFSA J 174:1–65
  • EFSA (European Food Safety Authority). (2009). Review of the existing maximum residue levels (MRLs) for ethephon on request of EFSA. EFSA J 7:1347–1391
  • EFSA (European Food Safety Authority). (2011). Review of the existing maximum residue levels (MRLs) for maleic hydrazide according to Article 12 of Regulation (EC) No 396/2005. EFSA J 9:2421 . doi:10.2903/j.efsa.2011.2421
  • EFSA (European Food Safety Authority). (2013). European Food Safety Authority. Scientific Opinion on the identification of pesticides to be included in cumulative assessment groups on the basis of their toxicological profile. EFSA J 11:3293
  • EPA (Environmental Protection Agency). (2001). Guidance Document on Using In Vitro Data to Estimate In Vivo Starting Doses for Acute Toxicity. NIH Publication No: 01–4500. Available from: http://www.epa.gov/hpv/pubs/general/nih2001b.pdf. Accessed on January 20, 2014
  • EPA (Environmental Protection Agency). (2006). Report of the Food Quality Protection Act (FQPA) Tolerance Reassessment and Risk Management Decision (TRED) for Ethephon. Publication No: EPA 738-R-05-005. Available from: www.epa.gov/oppsrrd1/REDs/ethephon_tred.pdf?. Accessed on January 20, 2014
  • Fotakis G, Timbrell JA. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 160:171–177
  • Haux JE, Quistad GB, Casida JE. (2000). Phosphobutyrylcholinesterase: phosphorylation of the esteratic site of butyrylcholinesterase by ethephon [(2-chloroethyl)phosphonic acid] dianion. Chem Res Toxicol 13:646–651
  • Haux JE, Lockridge O, Casida JE. (2002). Specificity of ethephon as a butyrylcholinesterase inhibitor and phosphorylating agent. Chem Res Toxicol 15:1527–1533
  • Horáková K, Sovcíková A, Seemannová Z, et al. (2001). Detection of drug-induced, superoxide-mediated cell damage and its prevention by antioxidants. Free Radic Biol Med 30:650–664
  • IPCS INCHEM. (1993). International Programme on Chemical Safety (IPCS). Ethephon. Available from: http://www.inchem.org/documents/jmpr/jmpmono/2002pr05.htm. Accessed on November 11, 2013
  • IPCS INCHEM. (2002). International Programme on Chemical Safety (IPCS). Pesticide residues in food - 2002 - Joint FAO/WHO Meeting on Pesticide Residues: Ethephon (addendum). Available from: http://www.inchem.org/documents/jmpr/jmpmono/2002pr05.htm. Accessed on November 11, 2013
  • Mamani Moreno C, Stadler T, da Silva AA, et al. (2012). Determination of maleic hydrazide residues in garlic bulbs by HPLC. Talanta 30:369–376
  • Marcano L, Carruyo I, Del Campo A, Montiel X. (2004). Cytotoxicity and mode of action of maleic hydrazide in root tips of Allium cepa L. Environ Res 94:221–226
  • Mays DL, Born GS, Christian JE, Liska BJ. (1968). Fate of C 14-maleic hydrazide in rats. J Agric Food Chem 16:356–357
  • Meschini R, Quaranta MT, Fiore M, et al. (1998). Chromosomal damage induced by maleic hydrazide in mammalian cells in vitro and in vivo. Mutat Res 204:645–648
  • Mosmann T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
  • Ozok N, Celik I. (2012). Effects of subacute and subchronic treatment of synthetic plant growth regulators on liver damage serum biomarkers tissue antioxidant defense systems and lipid peroxidation in rats. J Drug Metab Toxicol 3:124–134
  • Panemangalore M, Dowla HA, Byers ME. (1999). Occupational exposure to agricultural chemicals: effect on the activities of some enzymes in the blood of farm workers. Int Arch Occup Environ Health 72:84–88
  • Ribas G, Frenzilli G, Barale R, Marcos R. (1995). Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay. Mutat Res 344:41–54
  • Ribas G, Surralles J, Carbonell E, et al. (1996). Genotoxicity of the herbicides alachlor and maleic hydrazide in cultured human lymphocytes. Mutagenesis 11:221–227
  • Toxnet, United States National Library of Medicine Toxicology Data Network. (2004). Available from: http://toxnet.nlm.nih.gov/cgibin/sis/search/a?dbs+hsdb:@term+@DOCNO+1162. Accessed on August 7, 2012
  • Ulukaya E, Ozdikicioglu F, Oral AY, Demirci M. (2008). The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested. Toxicol In Vitro 22:232–239
  • US EPA. (2005). Report of the Food Quality Protection Act (FQPA) Tolerance Reassessment Progress and Risk Management Decision (TRED) for Maleic Hydrazide. Available from: http:www.epa.gov/oppsrrd1/REDs/maleic_hydrazide_tred.pdf. Accessed on September 19, 2012
  • US EPA. (2006). Report of the Food Quality Protection Act (FQPA) Tolerance Reassessment and Risk Management Decision (TRED) for Ethephon. Available from: http:www.epa.gov/oppsrrd1/REDs/ethephon_tred.pdf. Accessed on September 19, 2012
  • Yazar S, Baydan E. (2008). The subchronic toxic effects of plant growth promoters in mice. Vet J Ankara Univ 55:17–21

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