20
Views
28
CrossRef citations to date
0
Altmetric
Original Article

Properties of Isolated Nonciliated Bronchiolar Cells from Mouse Lung

, , &
Pages 553-573 | Received 29 Jan 1988, Accepted 07 Dec 1988, Published online: 02 Jul 2009

References

  • Clara M. Zur histobiologie des bronchalepithels. Z M Krosk Anat Forsch 1937; 41: 321–347
  • Kuhn C. The cells of the lungs and their organelles. The Biochemical Basis of Pulmonary Function, R G Crystal. Marcel Dekker, New York 1976; 3–48
  • Massaro G D, Paris M N, Thet L A. In vivo regulation of secretion of bronchiolar Clara cells in rats. J Clin Invest 1979; 63: 167–172
  • Massaro G D, Fischman C., Chiang M-J, Amado C., Massaro D. Regulation of secretion in Clara cells studied using the isolated perfused rat lung. J Clin Invest 1981; 67: 345–351
  • Boyd M R. Evidence for the Clara cell as a site of cytochrome P-450 dependent mixed function oxidase activity in lung. Nature (Lond) 1977; 269: 713–715
  • Devereux T R, Serabjit-Singh C J, Slaughter S R, Wolf C R, Philpot R., Fouts J R. Identification of cytochrome P-450 isozymes in nonciliated bronchiolar epithelial (Clara) and alveolar type II cells isolated from rabbit lung. Exp Lung Res 1981; 2: 221–230
  • Jones K G, Holland J F, Fouts J R. Benzo(a)pyrene hydrolase activity in enriched populations of Clara cells and alveolar type II cells from control and β-naphthoflavone-pretreated rats. CancerRes 1982; 42: 4658–4663
  • Devereux T R. Alveolar type II and Clara cells: Isolation and xenobiotic metabolism. Environ Health Perspect 1984; 56: 95–101
  • Evans M J, Cabral-Anderson L J, Freeman G. Role of the Clara cell in renewal of the bronchiolar epithelium. Lab Invest 1978; 38: 648–644
  • Haroldsen P E, Voelkel N F, Henson J E, Henson P M, Murphy R C. Metabolism of platelet-activating factor in isolated perfused rat lung. J Clin Invest 1987; 79: 1860–1867
  • Niden A H. Bronchiolar and large alveolar cell in pulmonary phospholipid metabolism. Science 1967; 158: 1323–1324
  • Smith P., Heath D., Moosaua H. The Clara cell. Thorax 1974; 29: 147–163
  • Petrik P., Collet A J. Quantitative electron microscopic autoradiography in vivo incorporation of 3H-choline, 3H-leucine, 3H-acetate and 3H-galactose in nonciliated bronchiolar (Clara) cells of mice. Am J Anat 1974; 139: 519–534
  • Kikkawa Y., Yoneda K. The type II epithelial cell of the lung. I. Method of isolation. Lab Invest 1974; 30: 76–84
  • Kikkawa Y., Yoneda K., Smith F., Packard B., Suzuki K. The type II epithelial cell of the lung. II. Chemical composition and phospholipid synthesis. Lab Invest 1975; 32: 295–302
  • Mason R J, Williams M C, Greenleaf R D, Clements J A. Isolation and properties of type II alveolar cells from rat lung. Am Rev Respir Dis 1977; 115: 1015–1026
  • Balis J U, Paterson J F, Paciga J E, Haller E., Shelley S A. Distribution and subcellu-lar localization of surfactant-associated glycoproteins in human lung. Lab Invest 1985; 52: 657–669
  • Walker S R, Williams M C, Benson B. Immunocytochemical localization of the major surfactant apoproteins in type II cells, Clara cells and alveolar macrophages of rat lung. J Histochem Cytochem 1986; 34: 1137–1148
  • Williams M C, Schenk D., Lewicki J., Benson B J, Hawgood S., Walker S R. Monoclonal antibodies against surfactant apoproteins label both alveolar type II cells and nonciliated bronchiolar cells in dog lung. J Cell Biol 1985; 101: 444a
  • Devereux T R, Fouts J R. Isolation and identification of Clara cells from rabbit lung. In Vitro 1980; 16: 958–968
  • Patton S E, Gilmore L B, Jetten A., Nettesheim P., Hook G ER. Biosynthesis and release of proteins by isolated pulmonary Clara cells. Exp Lung Res 1986; 11: 277–294
  • Massey T E, Geddes B A. Isolation of nonciliated bronchiolar epithelial (Clara) cells and alveolar type II cells from mouse lungs. Can J Physiol Pharmacol 1987; 65: 2368–2372
  • Walker S R, Hale S G, Malkinson A., Park S S, Mason R J. Isolation of non-ciliated bronchiolar epithelial cells from mouse lung. J Cell Biol 1986; 102: 207a
  • Phillips H J. Dye exclusion tests for cell viability. Tissue Culture Methods and Applications, P E Kruse, Jr, M K Patterson, Jr. Academic Press, New York, 406–408
  • Dobbs L G, Geppert E F, Williams M C, Greenleaf R D, Mason R J. Metabolic properties and ultrastructure of alveolar type II cells isolated from elastase. Biochim Biophys Am 1980; 618: 510–523
  • Johnson L V, Walsh M L, Bockus B J, Chen L B. Monitoring of relative mitochon-drial membrane potential in living cells by fluorescence microscopy. J Cell Biol 1981; 88: 526–535
  • Park S S, Fujino T., West D., Guengerich F P, Gelboin H V. Monoclonal antibodies that inhibit enzyme activity of 3 methylcholanthrene-induced cytochrome P-450. Cancer Res 1982; 42: 1798–1808
  • Park S S, Fujino T., Miller H., Guengerich F P, Gelboin H V. Monoclonal antibodies to phenobarbital induced rat liver cytochrome P-450. Biochem Pharmacol 1984; 33: 2071–2081
  • Bligh E G, Dyer W S. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37: 911–917
  • Fanestil D D, Barrows C H, Jr. Aging in the rotifer. J Gerontol 1965; 20: 462–469
  • Poorheis B J, Yazaki P J, Hostetler K Y. An improved two dimensional thin layer chromatography system for the separation of phosphatidylglycerol and its derivatives. J Lipid Res 1976; 17: 433–437
  • Dobbs L G, Mason R J, Williams M C, Benson B J, Sueishi K. Secretion of surfactant by primary cultures of alveolar type II cells isolated from rats. Biochim Biophys Acta 1982; 713: 118–127
  • Robinson P C, Voelker D R, Mason R J. Isolation and culture of human alveolar type II epithelial cells. Characterization of their phospholipid secretion. Am Rev Respir Dis 1984; 130: 1156–1160
  • Touchstone J C, Chen J C, Beaver K M. Improved separation of phospholipids in thin layer chromatography. Lipids 1980; 15: 61–62
  • Esko J D, Raetz C RH. Mutants of Chinese hamster ovary cells with altered membrane phospholipid composition. J Biol Chem 1980; 255: 4474–4480
  • Dobbs L G, Gonzalez R., Williams M C. An improved method for isolating type II cells in high yield and purity. Am Rev Respir Dis 1986; 134: 141–145
  • Plopper C G, Mariassy A T, Hill L H. Ultrastructure of the nonciliated bronchiolar epithelial (Clara) cell of mammalian lung. I. A comparison of rabbit, guinea pig, rat, hamster and mouse. Exp Lung Res 1980; 1: 139–154
  • Plopper C G, Mariassy A T, Hill L H. Ultrastructure of the nonciliated bronchiolar epithelial (Clara) cell of mammalian lung. II. A comparison of horse, steer, sheep, dog, and cat. Exp Lung Res 1980; 1: 155–169
  • Plopper C G, Hill L H, Mariassay A T. Ultrastructure of the nonciliated bronchiolar epithelial (Clara) cell of mammalian lung. III. A study of man with comparison of 15 mammalian species. Exp Lung Res 1980; 1: 171–180
  • Engel S. Comparative anatomy of respiratory tissue. Lung Structure. Charles C. Thomas, Springfield, IL 1962
  • Weibel E R. Morphometry of the human lung: The state of the art after two decades. Bull Eur Physiopathol Respir 1979; 15: 999–1013
  • Azzopardi A., Thurlbeck W M. The histochemistry of the non-ciliated bronchiolar epithelial cell. Am Rev Respir Dis 1969; 99: 516–525
  • Cutz E., Conen P E. Ultrastructure and cytochemistry of Clara cells. Am J Pathol 1971; 62: 127–134
  • Singh G., Katyal S L. An immunologic study of the secretory products of rat Clara cells. J Histochem Cytochem 1984; 32: 49–54
  • Singh G., Katyal S L, Gottron S A. Antigenic molecular and functional heterogeneity of Clara cell secretory proteins in the rat. Biochim Biophys Acta 1985; 829: 156–163
  • Singh G., Katyal S L, Wong-Chong M L. A quantitative assay for a Clara cell-specific protein and its application in the study of development of pulmonary airways in the rat. Pediatr Res 1986; 20: 802–805
  • Dobbs L G, Mason R J. Stimulation of secretion of disaturated phosphatidylcholine from isolated alveolar type II cells by 12-O-tetradecanoyl-13-phorbol acetate. Am Rev Respir Dis 1978; 118: 705–713
  • Dobbs L G, Mason R J. Pulmonary alveolar type II cells isolated from rats. Release of phosphatidylcholine in response to beta-adrenergic stimulation. J Clin Invest 1979; 63: 378–387

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.