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Ultrastructural Pathology Volume 34, 2010 - Issue 4
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Article

Trypsin Digestion on Paraffin Sections Is a Useful Tool for Diagnosis of Alport Syndrome

Dr. Ying Song Jiang Department of Histopathology, Shinshu University School of Medicine, Matsumoto, Japan
&
Dr. Takashi Ehara Department of Histopathology, Shinshu University School of Medicine, Matsumoto, JapanCorrespondence[email protected]
Pages 199-206 | Received 10 Jan 2010, Accepted 23 Feb 2010, Published online: 01 Jul 2010

References

  • Hostikka SL, Eddy RL, Byers MG, Höyhtyä M, Shows TB, Tryggvason K. Identification of a distinct type IV collagen α chain with restricted kidney distribution and assignment of its gene to the locus of X chromosome-linked Alport syndrome. Proc Natl Acad Sci USA. 1990;87:1606–1610.
  • Kashtan CE, Michael AF. Perspectives in clinical nephrology: Alport syndrome. Kidney Int. 1996;50:1445–1463.
  • Mariyama M, Zheng K, Yang-Feng TL, Reeders ST. Colocalization of the genes for the alpha 3(IV) and alpha 4(IV) chains of type IV collagen to chromosome 2 bands q35-q37. Genomics. 1992;13:809–813.
  • Pirson Y. Making the diagnosis of Alport's syndrome. Kidney Int. 1999;56:760–775.
  • Kagawa M, Kishiro Y, Naito I, et al. Epitope–defined monoclonal antibodies against type IV collagen for diagnosis of Alport's syndrome. Nephrol Dial Transplant. 1997;12:1238–1241.
  • Yoshioka K, Hino S, Takemura T, et al. Type IV collagen α5 chain normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody. Am J Pathol. 1994;144:986–996.
  • Krenacs L, Krenacs T, Raffeld M. Antigen retrieval for immunohistochemical reactions in routinely processed. Methods Mol Biol. 1999;115:85–93.
  • Gregory MC, Terreros DA, Barker DF, Fain PN, Denison JC, Atkin CL. Alport syndrome–clinical phenotypes, incidence, and pathology. Contrib Nephrol. 1996;117:1–28.
  • Chowdhury AR, Ehara T, Higuchi M, Hora K, Shigematsu H. Immunohistochemical detection of immunoglobulins and complements in formaldehyde-fixed and paraffin-embedded renal biopsy tissues; an adjunct for diagnosis of glomerulonephritis. Nephrology (Carlton). 2005;10:298–304.
  • Huang SN, Minassian H, More JD. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest. 1976;35:383–390.
  • Choi YJ, Reiner L. Immunofluorescence of renal lesions in paraffin-embedded and fresh-frozen sections. Am J Clin Pathol. 1980;73:116–119.
  • Naito I, Ninomiya Y, Nomura S. Immunohistochemical diagnosis of Alport's syndrome in paraffin-embedded renal sections: antigen retrieval with autoclave heating. Med Electron Microsc. 2003;36:1–7.
  • Guan N, Yu LX, Wu GH, Xing Y, Ding J. Antigen retrieval with protease digestion applied in immunohistochemical diagnosis of Alport syndrome. Nephrol Dial Transplant. 2008;23:3509–3513.
  • Naito I, Kawai S, Nomura S, Sado Y, Osawa G. Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome. Japanese Alport Network. Kidney Int. 1996;50:304–311.
  • Mori Y, Yamaguchi H, Seyama Y et al. Antigen retrieval in glomerulonephritis in immunohistochemistry. Pathol Clin Med. 1998;12:1581–1584.
  • Szekeres G, Lutz Y, Le Tourneau A, Delaage M. Steroid hormone receptor immunostaining on paraffin sections with microwave heating and trypsin digestion. J. Histotechnol. 1994;17:321–324.
  • Lyon MF. X-chromosome inactivation and developmental patterns in mammals. Biol Rev Camb Philos Soc. 1972;47:1–35.
  • Rao VH, Lees GE, Kashtan CE, et al. Increased expression of MMP-2, MMP-9 (type IV collagenases/gelatinases), and MT-1 MMP in canine X-linked Alport syndrome (XLAS). Kidney Int. 2003;63:1736–1748.
  • Suzanne Meleg-Smith MD. Alport disease: a review of the diagnostic difficulties. Ultrastruct Pathol. 2001;25:193–200.

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