17
Views
16
CrossRef citations to date
0
Altmetric
Original Article

Diagnostic Immunoelectron Microscopy in Surgical Pathology: Assessment of Various Tissue Fixation and Processing Protocols

, , , &
Pages 547-556 | Received 05 Jan 1993, Accepted 05 Jan 1993, Published online: 10 Jul 2009

References

  • Fanning J C, White J F, Polewski R, Cleary E G. Immunoelectron microscopic localization of elastic tissue components in archival tissue samples. J Microsc. 1991; 162: 355–367
  • Carlemalm E, Garavito R M, Villiger W. Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc. 1982; 126: 123–143
  • Taatjes D J, Arendash-Durand B, von Turkovich M, Trainer T D. HMB-45 antibody demonstrates melanosome-specificity by immuno-electron microscopy. Arch Pathol Lab Med. 1993; 117: 264–268
  • Roth J, Bendayan M, Carlemalm E, Villiger W, Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981; 29: 663–671
  • Roth J, Bendayan M, Orci L. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978; 26: 1074–1081
  • Roth J. The protein A-gold (pAg) technique. A qualitative and quantitative approach for antigen localization on thin sections. Techniques in Immunocytochemistry, G R Bullock, P Petrusz. Academic Press, London 1982; vol 1: 108–133
  • Roth J, Taatjes D J, Warhol M J. Prevention of non-specific interactions of gold-labeled reagents on tissue sections. Histochemistry. 1989; 92: 47–56
  • Frens G. Controlled nucleation for the regulation of particle size in monodisperse gold suspensions. Nature Phys Sci. 1973; 241: 20–22
  • Scopsi L, Migliari M, Vergani B, Lombardi L. The use of fixed-frozen tissue in routine ultra-structural evaluation and immunogold labeling. Ultrastruct Pathol. 1992; 16: 351–362
  • Deftos L, Chromogranin J. A. Its role in endocrine function and as an endocrine and nouroendocrine tumor marker. Endocrine Rev. 1991; 12: 181–187
  • Wiedenmann B, Huttner W B. Synaptophysin and chromogranins/secretogranins. Widespread constituents of distinct types of neuroendocrine vesicles and new tools in tumor diagnosis. Virchows Arch [B]. 1989; 58: 95–121
  • Varndell I M, Lloyd R C, Wilson B S, Polack J M. Ultrastructural localization of chromogranin. A potential marker for the electron microscopical recognition of endocrine cell secretory granules. Histochem. J. 1985; 17: 981–992
  • Hearn S A. Electron microscopic localization of chromogranin A in osmium-fixed neuroendocrine cells with a protein-A gold technique. J Histochem Cytochem. 1987; 35: 795–801
  • Abrahamsson P A, Falkmer S, Fält K, Grimelius L. The course of neuroendocrine differentiation in prostatic carcinomas. An immunohistochemical study testing chromogranin A as an “endocrine marker”?. Pathol Res Pract. 1989; 185: 373–380
  • Tötsch M, Müller L C, Hittmair A, Ofner D, Gibbs A R, Schmid K W. Immunohistochemical demonstration of chromogranins A and B in neuroendocrine tumors of the lung. Hum Pathol. 1992; 23: 312–316
  • Wick M R, Berg L C, Hertz M I. Large cell carcinoma of the lung with neuroendocrine differentiation. A comparison with large cell “undifferentiated” pulmonary tumors. Am J Clin Pathol. 1992; 97: 796–805
  • Staren E D, Gould V E, Warren W H, Wool N L, Bines S, Baker J, Bonomi P, Roseman D L, Economou S G. Neuroendocrine carcinomas of the colon and rectum. A clinicopathologic evaluation. Surgery. 1988; 104: 1080–1089
  • Scopsi L, Andreola S, Pilotti S, Testori A, Baldini M T, Leoni F, Lombardi L, Hutton J C, Shimizu F, Rosa P, Huttner W B, Rilke F. Argyrophilia and granin (chromogranin/secretogranin) expression in female breast carcinomas. Their relationship to survival and other disease parameters. Am J Surg Pathol. 1992; 16: 561–576
  • Graziano S L, Mazid R, Newman N, Tatum A, Oler A, Mortimer J A, Gullo J J, GiFino S M, Scalzo A J. The use of neuroendocrine immunoperoxidase markers to predict chemotherapy response in patients with non-small-cell lung cancer. J Clin Oncol. 1989; 7: 1398–1406

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.