In Vivo Confocal Microscopy of the Ocular Surface: From Bench to Bedside

References

• Niederer RL, McGhee CN. Clinical in vivo confocal microscopy of the human cornea in health and disease. Prog Retin Eye Res 2010;29:30–58
   PubMed Web of Science ®Google Scholar
• Guthoff RF, Zhivov A, Stachs O. In vivo confocal microscopy, an inner vision of the cornea – a major review. Clin Experiment Ophthalmol 2009;37:100–117
   PubMed Web of Science ®Google Scholar
• McLaren JW, Nau CB, Patel SV, Bourne WM. Measuring corneal thickness with the ConfoScan 4 and Z-ring adapter. Eye Contact Lens 2007;33:185–190
   PubMedGoogle Scholar
• Thomas PA, Geraldine P. Infectious keratitis. CurrOpin Infect Dis 2007;20:129–141
   PubMed Web of Science ®Google Scholar
• Keay L, Edwards K, Naduvilath T, Taylor HR, Snibson GR, Forde K, et al. Microbial keratitis predisposing factors and morbidity. Ophthalmology 2006;113:109–116
   PubMed Web of Science ®Google Scholar
• Kumar RL, Cruzat A, Hamrah P. Current state of in vivo confocal microscopy in management of microbial keratitis. Semin Ophthalmol 2010;25:166-170
   PubMed Web of Science ®Google Scholar
• Matsumoto Y, Dogru M, Sato EA, Katono Y, Uchino Y, Shimmura S, et al. The application of in vivo confocal scanning laser microscopy in the management of acanthamoeba keratitis. Mol Vis 2007;13:1319–1326
   PubMed Web of Science ®Google Scholar
• Labbé A, Khammari C, Dupas B, Gabison E, Brasnu E, Labetoulle M, et al. Contribution of in vivo confocal microscopy to the diagnosis and management of infectious keratitis. Ocul Surf 2009;7:41–52
   PubMed Web of Science ®Google Scholar
• Claerhout I, Goegebuer A, Van Den Broecke C, Kestelyn P. Delay in diagnosis and outcome of acanthamoeba keratitis. Graefes Arch Clin Exp Ophthalmol 2004;242:648–653
   PubMed Web of Science ®Google Scholar
• Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R. Confirmation of confocal microscopy diagnosis of acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 2000;118:178–183
   PubMedGoogle Scholar
• Lee HJ, Alipour F, Cruzat A, Zheng L, Hamrah P. In vivo confocal microscopy in diagnosis and management of acanthamoeba keratitis improves patient outcome. Invest Ophthalmol Vis Sci 2010;51:ARVO E-Abstract No. 2891
   Google Scholar
• Chew SJ, Feuerman RW, Assouline M, Kaufman HE, Barron BA, Hill JM. Early diagnosis of infectious keratitis with in vivo real time confocal microscopy. CLAO J 1992;18:197–201
   PubMedGoogle Scholar
• Kaufman SC, Musch DC, Belin MW, Cohen EJ, Meisler DM, Reinhart WJ, et al. Confocal microscopy: a report by the American Academy of Ophthalmology. Ophthalmology 2004;111:396–406
   PubMed Web of Science ®Google Scholar
• Kanavi MR, Javadi M, Yazdani S, Mirdehghanm S. Sensitivity and specificity of confocal scan in the diagnosis of infectious keratitis. Cornea 2007;26:782–786
   PubMed Web of Science ®Google Scholar
• Tu EY, Joslin CE, Sugar J, Booton GC, Shoff ME, Fuerst PA. The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of acanthamoeba keratitis. Cornea 2008;27:764–772
   PubMed Web of Science ®Google Scholar
• Vaddavalli PK, Garg P, Sharma S, Sangwan VS, Rao GN, Thomas R. Role of confocal microscopy in the diagnosis of fungal and acanthamoeba keratitis. Ophthalmology 2011;118:29–35
   PubMed Web of Science ®Google Scholar
• Hau SC, Dart JK, Vesaluoma M, Parmar DN, Claerhout I, Bibi K, et al. Diagnostic accuracy of microbial keratitis with in vivo scanning laser confocal microscopy. Br J Ophthalmol 2010;94:982–987
   PubMed Web of Science ®Google Scholar
• Baniasadi N, Cruzat A, Witkin D, Stacey R, Jakobiec FA, Chodosh J, et al. In vivo confocal microscopy for Paecilomyces lilacinus and Candida parapsilosis fungal keratitis. Invest Ophthalmol Vis Sci 2011;52:ARVO E-Abstract No. 5856
   Google Scholar
• Kurbanyan K, Hoesl LM, Schrems WA, Hamrah P. Corneal nerve alterations in acute acanthamoeba and fungal keratitis: an in vivo confocal microscopy study. Eye 2012;26:126–132
   PubMedGoogle Scholar
• Cruzat A, Witkin D, Baniasadi N, Zheng L, Ciolino JB, Jurkunas UV, et al. Inflammation and the nervous system: the connection in the cornea in patients with infectious keratitis. Invest Ophthalmol Vis Sci 2011;52:5136–5143
   PubMed Web of Science ®Google Scholar
• Hollingsworth JG, Efron N, Tullo AB. A longitudinal case series investigating cellular changes to the transplanted cornea using confocal microscopy. Cont Lens Anterior Eye 2006;29:135–141
   PubMedGoogle Scholar
• Patel SV, McLaren JW, Hodge DO, Bourne WM. The effect of corneal light scatter on vision after penetrating keratoplasty. Am J Ophthalmol 2008;146:913–919
   PubMed Web of Science ®Google Scholar
• Patel SV, Erie JC, McLaren JW, Bourne WM. Keratocyte density and recovery of subbasal nerves after penetrating keratoplasty and in late endothelial failure. Arch Ophthalmol 2007;125:1693–1698
   PubMedGoogle Scholar
• Tan DTH, Mehta JS. Future directions in lamellar corneal transplantation. Cornea 2007;26:S21–S28
   PubMed Web of Science ®Google Scholar
• McLaren JW, Patel SV. Modeling the effect of forward scatter and aberrations on visual acuity after endothelial keratoplasty. Invest Ophthalmol Vis Sci 2012;53:5545–5551
   PubMedGoogle Scholar
• McLaren JW, Bourne WM, Patel SV. Standardization of corneal haze measurement in confocal microscopy. Invest Ophthalmol Vis Sci 2010;51:5610–5616
   PubMed Web of Science ®Google Scholar
• Baratz KH, McLaren JW, Maguire LJ, Patel SV. Corneal haze determined by confocal microscopy 2 years after Descemet stripping with endothelial keratoplasty for Fuchs corneal dystrophy. Arch Ophthalmol 2012;130:868–874
   PubMedGoogle Scholar
• Patel SV, Baratz KH, Hodge DO, Maguire LJ, McLaren JW. The effect of corneal light scatter on vision after Descemet stripping with endothelial keratoplasty. Arch Ophthalmol 2009;127:153–160
   PubMedGoogle Scholar
• Patel SV, McLaren JW. In vivo confocal microscopy of Fuchs endothelial dystrophy before and after endothelial keratoplasty. JAMA Ophthalmol 2013;131:611–618
   PubMedGoogle Scholar
• van der Meulen IJ, Patel SV, Lapid-Gortzak R, Nieuwendaal CP, McLaren JW, van den Berg TJ. Quality of vision in patients with Fuchs endothelial dystrophy and after Descemet stripping endothelial keratoplasty. Arch Ophthalmol 2011;129:1537–1542
   PubMedGoogle Scholar
• Ahuja Y, Baratz KH, McLaren JW, Bourne WM, Patel SV. Decreased corneal sensitivity and abnormal corneal nerves in Fuchs endothelial dystrophy. Cornea 2012;31:1257–1263
   PubMed Web of Science ®Google Scholar
• Al-Aqaba M, Alomar T, Lowe J, Dua HS. Corneal nerve aberrations in bullous keratopathy. Am J Ophthalmol 2011;151:840–849
   PubMedGoogle Scholar
• Patel SV, Baratz KH, Maguire LJ, Hodge DO, McLaren JW. Anterior corneal aberrations after Descemet stripping endothelial keratoplasty for Fuchs endothelial dystrophy. Ophthalmology 2012;119:1522–1529
   PubMed Web of Science ®Google Scholar
• Hecker LA, McLaren JW, Bachman LA, Patel SV. Anterior keratocyte depletion in Fuchs endothelial dystrophy. Arch Ophthalmol 2011;129:555–561
   PubMedGoogle Scholar
• Raecker ME, McLaren JW, Kittleson KM, Patel SV. Endothelial image quality after Descemet stripping with endothelial keratoplasty: a comparison of three microscopy techniques. Eye Contact Lens 2011;37:6–10
   PubMedGoogle Scholar
• Seery LS, Nau CB, McLaren JW, Baratz KH, Patel SV. Graft thickness, graft folds, and aberrations after Descemet stripping endothelial keratoplasty for Fuchs dystrophy. Am J Ophthalmol 2011;152:910–916
   PubMedGoogle Scholar
• Shortt AJ, Allan BD, Evans JR. Laser-assisted in-situ keratomileusis (LASIK) versus photorefractive keratectomy (PRK) for myopia. Cochrane Database Syst Rev 2013;1:CD005135
   PubMed Web of Science ®Google Scholar
• McLaren JW, Bourne WM, Patel SV. Stromal reflectance after photorefractive keratectomy: a paired comparison between epithelial removal by rotary brush and excimer laser-scrape. Invest Ophthalmol Vis Sci 2012;53:AVRO E-Abstract No. 1469
   Google Scholar
• Erie JC, Patel SV, McLaren JW, Hodge DO, Bourne WM. Corneal keratocyte deficits after photorefractive keratectomy and laser in situ keratomileusis. Am J Ophthalmol 2006;141:799–809
   PubMed Web of Science ®Google Scholar
• Calvo R, McLaren JW, Hodge DO, Bourne WM, Patel SV. Corneal aberrations and visual acuity after laser in situ keratomileusis: femtosecond laser versus mechanical microkeratome. Am J Ophthalmol 2010;149:785–793
   PubMedGoogle Scholar
• Erie JC, McLaren JW, Hodge DO, Bourne WM. Recovery of corneal subbasal nerve density after PRK and LASIK. Am J Ophthalmol 2005;140:1059–1064
   PubMed Web of Science ®Google Scholar
• Patel SV, McLaren JW, Kittleson KM, Bourne WM. Subbasal nerve density and corneal sensitivity after laser in situ keratomileusis: femtosecond laser versus mechanical microkeratome. Arch Ophthalmol 2010;128:1413–1419
   PubMedGoogle Scholar
• Klingler KN, McLaren JW, Bourne WM, Patel SV. Corneal endothelial cell changes 5 years after laser in situ keratomileusis: femtosecond laser versus mechanical microkeratome. J Cataract Refract Surg 2012;38:2125–2130
   PubMed Web of Science ®Google Scholar
• Patel SV, Bourne WM. Corneal endothelial cell loss 9 years after excimer laser keratorefractive surgery. Arch Ophthalmol 2009;127:1423–1427
   PubMedGoogle Scholar
• International Dry Eye WorkShop (DEWS). The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye Workshop (2007). Ocul Surf 2007;5:75–92
   PubMed Web of Science ®Google Scholar
• Nichols KK, Foulks GN, Bron AJ, Glasgow BJ, Dogru M, Tsubota K, et al. The international workshop on meibomian gland dysfunction: executive summary. Invest Ophthalmol Vis Sci 2011;52:1922–1929
   PubMed Web of Science ®Google Scholar
• Alhatem A, Cavalcanti B, Hamrah P. In vivo confocal microscopy in dry eye disease and related conditions. Semin Ophthalmol 2012;27:138–148
   PubMed Web of Science ®Google Scholar
• Villani E, Magnani F, Viola F, Santaniello A, Scorza R, Nucci P, et al. In vivo confocal evaluation of the ocular surface morpho-functional unit in dry eye. Optom Vis Sci 2013;90:576–586
   PubMed Web of Science ®Google Scholar
• Tuominen IS, Konttinen YT, Vesaluoma MH, Moilanen JA, Helintö M, Tervo TM. Corneal innervation and morphology in primary Sjögren’s syndrome. Invest Ophthalmol Vis Sci 2003;44:2545–2549
   PubMed Web of Science ®Google Scholar
• Villani E, Galimberti D, Viola F, Mapelli C, Ratiglia R. The cornea in Sjogren’s syndrome: an in vivo confocal study. Invest Ophthalmol Vis Sci 2007;48:2017–2022
   PubMed Web of Science ®Google Scholar
• Villani E, Galimberti D, Viola F, Mapelli C, Del Papa N, Ratiglia R. Corneal involvement in rheumatoid arthritis: an in vivo confocal study. Invest Ophthalmol Vis Sci 2008;49:560-564
   PubMed Web of Science ®Google Scholar
• Zhang X, Chen Q, Chen W, Cui L, Ma H, Lu F. Tear dynamics and corneal confocal microscopy of subjects with mild self-reported office dry eye. Ophthalmology 2011;118:902–907
   PubMed Web of Science ®Google Scholar
• Efron N. Contact lens-induced changes in the anterior eye as observed in vivo with the confocal microscope. Prog Retin Eye Res 2007;26:398–436
   PubMed Web of Science ®Google Scholar
• Villani E, Viola F, Sala R, Salvi M, Mapelli C, Currò N, et al. Corneal involvement in Graves’ orbitopathy: an in vivo confocal study. Invest Ophthalmol Vis Sci 2010;51:4574–4578
   PubMed Web of Science ®Google Scholar
• Benítez del Castillo JM, Acosta MC, Wassfi MA, Díaz-Valle D, Gegúndez JA, Fernandez C, et al. Relation between corneal innervation with confocal microscopy and corneal sensitivity with noncontact esthesiometry in patients with dry eye. Invest Ophthalmol Vis Sci 2007;48:173–181
   PubMed Web of Science ®Google Scholar
• Tuisku IS, Konttinen YT, Konttinen LM, Tervo TM. Alterations in corneal sensitivity and nerve morphology in patients with primary Sjögren’s syndrome. Exp Eye Res 2008;86:879–885
   PubMed Web of Science ®Google Scholar
• Hoşal BM, Ornek N, Zilelioğlu G, Elhan AH. Morphology of corneal nerves and corneal sensation in dry eye: a preliminary study. Eye 2005;19:1276–1279
   PubMed Web of Science ®Google Scholar
• Zhang M, Chen J, Luo L, Xiao Q, Sun M, Liu Z. Altered corneal nerves in aqueous tear deficiency viewed by in vivo confocal microscopy. Cornea 2005;24:818–824
   PubMed Web of Science ®Google Scholar
• Cruzat A, Pavan-Langston D, Hamrah P. In vivo confocal microscopy of corneal nerves—analysis and clinical correlation. Semin Ophthalmol 2010;25:171–177
   PubMed Web of Science ®Google Scholar
• Lin H, Li W, Dong N, Chen W, Liu J, Chen L, et al. Changes in corneal epithelial layer inflammatory cells in aqueous tear-deficient dry eye. Invest Ophthalmol Vis Sci 2010;51:122–128
   PubMed Web of Science ®Google Scholar
• Villani E, Galimberti D, Del Papa N, Nucci P, Ratiglia R. Inflammation in dry eye associated with rheumatoid arthritis: cytokine and in vivo confocal microscopy study. Innate Immun 2013;19:420–427
   PubMed Web of Science ®Google Scholar
• Matsumoto Y, Sato EA, Ibrahim OM, Dogru M, Tsubota K. The application of in vivo laser confocal microscopy to the diagnosis and evaluation of meibomian gland dysfunction. Mol Vis 2008;14:1263–1271
   PubMed Web of Science ®Google Scholar
• Ibrahim OM, Matsumoto Y, Dogru M, Adan ES, Wakamatsu TH, Goto T, et al. The efficacy, sensitivity, and specificity of in vivo laser confocal microscopy in the diagnosis of meibomian gland dysfunction. Ophthalmology 2010;117:665–672
   PubMed Web of Science ®Google Scholar
• Villani E, Beretta S, De Capitani M, Galimberti D, Viola F, Ratiglia R. In vivo confocal microscopy of meibomian glands in Sjögren’s syndrome. Invest Ophthalmol Vis Sci 2011;52:933–939
   PubMed Web of Science ®Google Scholar
• Villani E, Ceresara G, Beretta S, Magnani F, Viola F, Ratiglia R. In vivo confocal microscopy of meibomian glands in contact lens wearers. Invest Ophthalmol Vis Sci 2011;52:5215–5219
   PubMed Web of Science ®Google Scholar
• Matsumoto Y, Shigeno Y, Sato EA, Ibrahim OM, Saiki M, Negishi K, et al. The evaluation of the treatment response in obstructive meibomian gland disease by in vivo laser confocal microscopy. Graefes Arch Clin Exp Ophthalmol 2009;247:821–829
   PubMed Web of Science ®Google Scholar
• Ban Y, Ogawa Y, Ibrahim OM, Tatematsu Y, Kamoi M, Uchino M, et al. Morphologic evaluation of meibomian glands in chronic graft-versus-host disease using in vivo laser confocal microscopy. Mol Vis 2011;17:2533–2543
   PubMed Web of Science ®Google Scholar
• Villani E, Canton V, Magnani F, Viola F, Nucci P, Ratiglia R. The aging meibomian gland: an in vivo confocal study. Invest Ophthalmol Vis Sci 2013;54:4735–4740
   PubMed Web of Science ®Google Scholar
• Kojima T, Matsumoto Y, Dogru M, Tsubota K. The application of in vivo laser scanning confocal microscopy as a tool of conjunctival in vivo cytology in the diagnosis of dry eye ocular surface disease. Mol Vis 2010;16:2457–2464
   PubMed Web of Science ®Google Scholar
• Villani E, Beretta S, Galimberti D, Viola F, Ratiglia R. In vivo confocal microscopy of conjunctival roundish bright objects: young, older, and Sjögren subjects. Invest Ophthalmol Vis Sci 2011;52:4829–4832
   PubMed Web of Science ®Google Scholar
• Wakamatsu TH, Sato EA, Matsumoto Y, Ibrahim OM, Dogru M, Kaido M, et al. Conjunctival in vivo confocal scanning laser microscopy in patients with Sjögren syndrome. Invest Ophthalmol Vis Sci 2010;51:144–150
   PubMed Web of Science ®Google Scholar
• Hong J, Zhu W, Zhuang H, Xu J, Sun X, Le Q, et al. In vivo confocal microscopy of conjunctival goblet cells in patients with Sjögren’s syndrome dry eye. Br J Ophthalmol 2010;94:1454–1458
   PubMed Web of Science ®Google Scholar
• Sato EA, Matsumoto Y, Dogru M, Kaido M, Wakamatsu T, Ibrahim OM, et al. Lacrimal gland in Sjögren’s syndrome. Ophthalmology 2010;117:1055–1055
   PubMedGoogle Scholar
• Villani E, Mantelli F, Nucci P. In-vivo confocal microscopy of the ocular surface: ocular allergy and dry eye. Curr Opin Allergy Clin Immunol 2013;13:569--576
   Google Scholar
• Kenchegowda S, Bazan HE. Significance of lipid mediators in corneal injury and repair. J Lipid Res 2010;51:879–891
   PubMed Web of Science ®Google Scholar
• Oliveira-Soto L, Efron N. Morphology of corneal nerves using confocal microscopy. Cornea 2001;20:374–384
   PubMed Web of Science ®Google Scholar
• Patel DV, McGhee CN. In vivo confocal microscopy of human corneal nerves in health, in ocular and systemic disease, and following corneal surgery: a review. Br J Ophthalmol 2009;93:853–860
   PubMed Web of Science ®Google Scholar
• Benítez del Castillo JM, Wasfy MA, Fernandez C, Garcia-Sanchez J. An in vivo confocal masked study on corneal epithelium and subbasal nerves in patients with dry eye. Invest Ophthalmol Vis Sci 2004;45:3030–3035
   PubMed Web of Science ®Google Scholar
• Lee SJ, Jin KK, Kyung YS, Kim EK, Lee HK. Comparison of corneal nerve regeneration and sensitivity between LASIK and laser epithelial keratomileusis (LASEK). Am J Ophthalmol 2006;141:1009–1015
   PubMed Web of Science ®Google Scholar
• Lee BH, McLaren JW, Erie JC, Hodge DO, Bourne WM. Reinnervation in the cornea after LASIK. Invest Ophthalmol Vis Sci 2002;43:3660–3664
   PubMed Web of Science ®Google Scholar
• Kallinikos P, Berhanu M, O’Donnell C, Boulton AJ, Efron N, Malik RA. Corneal nerve tortuosity in diabetic patients with neuropathy. Invest Ophthalmol Vis Sci 2004;45:418–422
   PubMed Web of Science ®Google Scholar
• Stapleton F, Hayward KB, Bachand N, Trong PH, Teh DW, Deng KM, et al. Evaluation of corneal sensitivity to mechanical and chemical stimuli after LASIK: a pilot study. Eye Contact Lens 2006;32:88–93
   PubMedGoogle Scholar
• Stachs O, Zhivov A, Kraak R, Hovakimyan M, Wree A, Guthoff R. Structural-functional correlations of corneal innervation after LASIK and penetrating keratoplasty. J Refrac Surg 2010;26:159–167
   PubMed Web of Science ®Google Scholar
• Tuisku IS, Lindbohm N, Wilson SE. Dry eye and corneal sensitivity after high myopic LASIK. J Refract Surg 2007;23:338–342
   PubMed Web of Science ®Google Scholar
• Rao K, Leveque C, Pflugfelder SC. Corneal nerve regeneration in neurotrophic keratopathy following autologous plasma therapy. Br J Ophthalmol 2010;94:584–591
   PubMed Web of Science ®Google Scholar
• Marfurt CF, Ellis LC, Jones MA. Sensory and sympathetic nerve sprouting in the rat cornea following neonatal administration of capsaicin. Somatosens Mot Res 1993;10:377–398
   PubMed Web of Science ®Google Scholar
• Albers KM, Wright DE, Davis BM. Overexpression of nerve growth factor in epidermis of transgenic mice causes hypertrophy of the peripheral nervous system. J Neurosci 1994;14:1422–1432
   PubMed Web of Science ®Google Scholar
• Ambrósio R Jr, Tervo T, Wilson SE. LASIK-associated dry eye and neurotrophic epitheliopathy: pathophysiology and strategies for prevention and treatment. J Refract Surg 2008;24:396–407
   PubMed Web of Science ®Google Scholar
• Rosenthal P, Baran I, Jacobs DS. Corneal pain without stain. Is it real? Ocul Surf 2009;7:28–40
   PubMed Web of Science ®Google Scholar
• Nettune GR, Pflugfelder SC. Post-LASIK tear dysfunction and dysesthesia. Ocular Surf 2010;8:135–145
   PubMed Web of Science ®Google Scholar
• Benitez-del-Castillo JM, del Rio T, Iradier T, Hernández JL, Castillo A, Garcia-Sanchez J. Decrease in tear secretion and corneal sensitivity after laser in situ keratomileusis. Cornea 2001;20:30–32
   PubMed Web of Science ®Google Scholar
• Battat L, Macri A, Dursun D, Pflugfelder SC. Effects of laser in situ keratomileusis on tear production, clearance, and the ocular surface. Ophthalmology 2001;108:1230–1235
   PubMed Web of Science ®Google Scholar
• Gallar J, Acosta MC, Moilanen JA, Holopainen JM, Belmonte C, Tervo TM. Recovery of corneal sensitivity to mechanical and chemical stimulation after laser in situ keratomileusis. J Refract Surg 2004;20:229–235
   PubMed Web of Science ®Google Scholar
• Mimura T, Amano S, Fukuoka S, Honda N, Arita R, Ochiai M, et al. In vivo confocal microscopy of hereditary sensory and autonomic neuropathy. Curr Eye Res 2008;33:940–945
   PubMed Web of Science ®Google Scholar
• Lalive PH, Truffert A, Magistris MR, Landis T, Dosso A. Peripheral autoimmune neuropathy assessed using corneal in vivo confocal microscopy. Arch Neurol 2009;66:403–405
   PubMed Web of Science ®Google Scholar
• Ferrari G, Gemignani F, Macaluso C. Chemotherapy-associated peripheral sensory neuropathy assessed using in vivo corneal confocal microscopy. Arch Neurol 2010;67:364–365
   PubMedGoogle Scholar
• American Diabetes Association. Data from the 2011 National Diabetes Fact Sheet (released 26 January 2011). Available at http://www.diabetes.org/diabetes-basics/diabetes-statistics/?loc=DropDownDB-stats [last accessed 15 July 2013]
   Google Scholar
• Abbott CA, Vileikyte L, Williamson S, Carrington AL, Boulton AJ. Multicenter study of the incidence of and predictive risk factors for diabetic neuropathic foot ulceration. Diabetes Care 1998;21:1071–1075
   PubMed Web of Science ®Google Scholar
• Tavakoli M, Quattrini C, Abbott CA, Kallinikos P, Marshall A, Finnigan J, et al. Corneal confocal microscopy: a novel noninvasive test to diagnose and stratify the severity of human diabetic neuropathy. Diabetes Care 2010;33:1792–1797
   PubMed Web of Science ®Google Scholar
• Rosenberg ME, Tervo TM, Immonen IJ, Müller LJ, Grönhagen-Riska C, Vesaluoma MH. Corneal structure and sensitivity in type 1 diabetes mellitus. Invest Ophthalmol Vis Sci 2000;41:2915–2921
   PubMed Web of Science ®Google Scholar
• Mocan MC, Durukan I, Irkec M, Orhan M. Morphologic alterations of both the stromal and subbasal nerves in the corneas of patients with diabetes. Cornea 2006;25:769–773
   PubMed Web of Science ®Google Scholar
• Ziegler D, Zhivov A, Allgeier S, Winter K, Papanas N, Ziegler I, et al. Early detection of nerve fiber loss by corneal confocal microscopy and skin biopsy in recently diagnosed type 2 diabetic subjects. Diabetologia 2012;55:A44
   Google Scholar
• Petropoulos IN, Alam U, Fadavi H, Asghar O, Green P, Ponirakis G, et al. Corneal nerve loss detected with corneal confocal microscopy is symmetrical and related to the severity of diabetic polyneuropathy. Diabetes Care 2013. [Epub ahead of print]
   Google Scholar
• Ahmed A, Bril V, Orszag A, Paulson J, Yeung E, Ngo M, et al. Detection of diabetic sensorimotor polyneuropathy by corneal confocal microscopy in type 1 diabetes: a concurrent validity study. Diabetes Care 2012;35:821–828
   PubMed Web of Science ®Google Scholar
• Pritchard N, Edwards K, Shahidi AM, Sampson GP, Russell AW, Malik RA, et al. Corneal markers of diabetic neuropathy. Ocul Surf 2011;9:17–28
   PubMed Web of Science ®Google Scholar
• Papanas N, Ziegler D. Corneal confocal microscopy: a new technique for early detection of diabetic neuropathy. Curr Diab Rep 2013;13:488–499
   PubMed Web of Science ®Google Scholar
• Labbe A, Dupas B, Hamard P, Baudouin C. In vivo confocal microscopy study of blebs after filtering surgery. Ophthalmology 2005;112:1979
   PubMed Web of Science ®Google Scholar
• Guthoff R, Klink T, Schlunck G, Grehn F. In vivo confocal microscopy of failing and functioning filtering blebs: results and clinical correlations. J Glaucoma 2006;15:552–558
   PubMed Web of Science ®Google Scholar
• Messmer EM, Zapp DM, Mackert MJ, Thiel M, Kampik A. In vivo confocal microscopy of filtering blebs after trabeculectomy. Arch Ophthalmol 2006;124:1095–1103
   PubMedGoogle Scholar
• Amar N, Labbe A, Hamard P, Dupas B, Baudouin C. Filtering blebs and aqueous pathway an immunocytological and in vivo confocal microscopy study. Ophthalmology 2008;115:1154–1161.e4
   PubMedGoogle Scholar
• Martone G, Frezzotti P, Tosi GM, Traversi C, Mittica V, Malandrini A, et al. An in vivo confocal microscopy analysis of effects of topical antiglaucoma therapy with preservative on corneal innervation and morphology. Am J Ophthalmol 2009;147:725–735
   PubMed Web of Science ®Google Scholar
• Baratz KH, Nau CB, Winter EJ, McLaren JW, Hodge DO, Herman DC, et al. Effects of glaucoma medications on corneal endothelium, keratocytes, and subbasal nerves among participants in the ocular hypertension treatment study. Cornea 2006;25:1046–1052
   PubMed Web of Science ®Google Scholar
• Labbe A, Alalwani H, Van Went C, Brasnu E, Georgescu D, Baudouin C. The relationship between subbasal nerve morphology and corneal sensation in ocular surface disease. Invest Ophthalmol Vis Sci 2012;53:4926–4931
   PubMed Web of Science ®Google Scholar
• Liang H, Brignole-Baudouin F, Riancho L, Baudouin C. Reduced in vivo ocular surface toxicity with polyquad-preserved travoprost versus benzalkonium-preserved travoprost or latanoprost ophthalmic solutions. Ophthalmic Res 2012;48:89–101
   PubMed Web of Science ®Google Scholar
• Labbe A, Pauly A, Liang H, Brignole-Baudouin F, Martin C, Warnet JM, et al. Comparison of toxicological profiles of benzalkonium chloride and polyquaternium-1: an experimental study. J Ocul Pharmacol Ther 2006;22:267–278
   PubMed Web of Science ®Google Scholar
• Liang H, Baudouin C, Labbe A, Riancho L, Brignole-Baudouin F. Conjunctiva-associated lymphoid tissue (CALT) reactions to antiglaucoma prostaglandins with or without BAK-preservative in rabbit acute toxicity study. PLoS One 2012;7:e33913
   PubMed Web of Science ®Google Scholar
• Ladage PM, Petroll WM, Jester JV, Fisher S, Bergmanson JP, Cavanagh HD. Spherical indentations of human and rabbit corneal epithelium following extended contact lens wear. CLAO J 2002;28:177–180
   PubMedGoogle Scholar
• Craig JP, Sherwin T, Grupcheva CN, McGhee CN. An evaluation of mucin balls associated with high-DK silicone-hydrogel contact lens wear. Adv Exp Med Biol 2002;506:917–923
   PubMedGoogle Scholar
• Millar TJ, Papas EB, Ozkan J, Jalbert I, Ball M. Clinical appearance and microscopic analysis of mucin balls associated with contact lens wear. Cornea 2003;22:740–745
   PubMed Web of Science ®Google Scholar
• Ladage PM, Yamamoto K, Ren DH, Li L, Jester JV, Petroll WM. Effects of rigid and soft contact lens daily wear on corneal epithelium, tear lactate dehydrogenase, and bacterial binding to exfoliated epithelial cells. Ophthalmology 2001;108:1279–1288
   PubMed Web of Science ®Google Scholar
• Imayasu M, Moriyama T, Ichijima H, Ohashi J, Petroll WM, Jester JV, et al. The effects of daily wear of rigid gas permeable contact lenses treated with contact lens care solutions containing preservatives on the rabbit cornea. CLAO J 1994;20:183–188
   PubMedGoogle Scholar
• Ren DH, Petroll WM, Jester JV, Ho-Fan J, Cavanagh HD. The relationship between contact lens oxygen permeability and binding of Pseudomonas aeruginosa to human corneal epithelial cells after overnight and extended wear. CLAO J 1999;25:80–100
   PubMedGoogle Scholar
• Kalogeropoulos G, Chang S, Bolton T, Jalbert I. The effects of short-term lens wear and eye rubbing on the corneal epithelium. Eye Contact Lens 2009;35:255–259
   PubMedGoogle Scholar
• Jalbert I, Sweeney DF, Stapleton F. The effect of long-term wear of soft lenses of low and high oxygen transmissibility on the corneal epithelium. Eye 2009;23:1282–1287
   PubMed Web of Science ®Google Scholar
• Bansal AK, Mustonen RK, McDonald MB. High resolution in vivo scanning confocal microscopy of the cornea in long term contact lens wear. Invest Ophthalmol Vis Sci 1997;38:ARVO Abstract S138
   Google Scholar
• Wu T, Ahmed A, Bril V, Orszag A, Ng E, Nwe P, et al. Variables associated with corneal confocal microscopy parameters in healthy volunteers: implications for diabetic neuropathy screening. Diabet Med 2012;29:e297–e303
   PubMed Web of Science ®Google Scholar
• Oliveira-Soto L, Efron N. Morphology of corneal nerves in soft contact lens wear. A comparative study using confocal microscopy. Ophthal Physiol Opt 2003;23:163–174
   PubMedGoogle Scholar
• Zhivov A, Stave J, Vollmar B, Guthoff R. In vivo confocal microscopic evaluation of langerhans cell density and distribution in the corneal epithelium of healthy volunteers and contact lens wearers. Cornea 2007;26:47–54
   PubMed Web of Science ®Google Scholar
• Sindt CW, Grout TK, Critser DB, Kern JR, Meadows DL. Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study. Clin Ophthalmol 2012;6:511–519
   PubMedGoogle Scholar
• Efron N, Mutalib HA, Perez-Gomez I, Koh HH. Confocal microscopic observations of the human cornea following overnight contact lens wear. Clin Exp Optom 2002;85:149–155
   PubMedGoogle Scholar
• Patel SV, McLaren JW, Hodge DO, Bourne WM. Confocal microscopy in vivo in corneas of long-term contact lens wearers. Invest Ophthalmol Vis Sci 2002;43:995–1003
   PubMed Web of Science ®Google Scholar
• Jalbert I, Stapleton F. Effect of lens wear on corneal stroma: preliminary findings. Aust N Z J Ophthalmol 1999;27:211–213
   PubMedGoogle Scholar
• Kallinikos P, Morgan PB, Efron N. Assessment of stromal keratocytes and tear film inflammatory mediators during extended wear of contact lenses. Cornea 2006;25:1–10
   PubMed Web of Science ®Google Scholar
• Weed KH, MacEwen CJ, Cox A, McGhee CN. Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy. Eye 2007;21:614–623
   PubMed Web of Science ®Google Scholar
• Zhong X, Chen X, Xie RZ, Yang J, Li S, Yang X, et al. Differences between overnight and long-term wear of orthokeratology contact lenses in corneal contour, thickness, and cell density. Cornea 2009;28:271–279
   PubMed Web of Science ®Google Scholar
• Yagmur M, Okay O, Sizmaz S, Unal I, Yar K. In vivo confocal microscopy: corneal changes of hydrogel contact lens wearers. Int Ophthalmol 2011;31:377–383
   PubMedGoogle Scholar
• Ohta K, Shimamura I, Shiraishi A, Ohashi Y. Confocal microscopic observations of stromal keratocytes in soft and rigid contact lens wearers. Cornea 2012;31:66–73
   PubMedGoogle Scholar
• Kallinikos P, Efron N. On the etiology of keratocyte loss during contact lens wear. Invest Ophthalmol Vis Sci 2004;45:3011–3020
   PubMed Web of Science ®Google Scholar
• Böhnke M, Masters BR. Long term contact lens wear induces a corneal degeneration with microdot deposits in the corneal stroma. Ophthalmology 1997;104:1887–1896
   PubMed Web of Science ®Google Scholar
• Trittibach P, Cadez R, Eschmann R, Sarra GM, Boehnke M, Frueh BE. Determination of microdot stromal degenerations within corneas of long-term contact lens wearers by confocal microscopy. Eye Contact Lens 2004;30:127–131
   PubMedGoogle Scholar
• Bastion ML, Mohamad MH. Study of the factors associated with the presence of white dots in the corneas of regular soft contact lens users from an Asian country. Eye Contact Lens 2006;32:223–227
   PubMedGoogle Scholar
• Efron N, Mutalib HA. Confocal microscopy observations of the cornea in response to contact lens wear. Die Kontaktlinse 2001;35:4–16
   Google Scholar
• Ma X, He L, He D, Xu J. Chloroquine keratopathy of rheumatoid arthritis patients detected by in vivo confocal microscopy. Curr Eye Res 2012;37:293–299
   PubMed Web of Science ®Google Scholar
• Sbeity Z, Palmiero PM, Tello C, Liebmann JM, Ritch R. Non-contact in vivo confocal scanning laser microscopy in exfoliation syndrome, exfoliation syndrome suspect and normal eyes. Acta Ophthalmol 2011;89:241–247
   PubMed Web of Science ®Google Scholar
• Mocan MC, Eldem B, Irkec M. In vivo confocal microscopic findings of two siblings with Maroteaux-Lamy syndrome. Cornea 2007;26:90–93
   PubMedGoogle Scholar
• Efron N. Endothelial blebs. Contact lens complications, 3rd edn. Edinburgh: Elsevier-Saunders; 2012
   Google Scholar
• Nagel S, Wiegand W, Thaer AA, Geyer OC. Light scattering study of the cornea in contact lens patients. In vivo studies using confocal slit scanning microscopy. Ophthalmologe 1996;93:252–256
   PubMedGoogle Scholar
• Rong B, Yan XM. Changes of corneal limbus in long-term soft contact lens wearers by using laser confocal microscope. Zhonghua Yan Ke Za Zhi 2007;43:514–518
   PubMedGoogle Scholar
• Nguyen TH, Dudek LT, Krisciunas TC, Matiaco P, Planck SR, Mathers WD, et al. In vivo confocal microscopy: increased conjunctival or episcleral leukocyte adhesion in patients who wear contact lenses with lower oxygen permeability (Dk) values. Cornea 2004;23:695–700
   PubMed Web of Science ®Google Scholar
• Sutphin JE, Kantor AL, Mathers WD, Mehaffey MG. Evaluation of infectious crystalline keratitis with confocal microscopy in a case series. Cornea 1997;16:21–26
   PubMedGoogle Scholar
• Su PY, Hu FR, Chen YM, Han JH, Chen WL. Dendritiform cells found in central cornea by in-vivo confocal microscopy in a patient with mixed bacterial keratitis. Ocul Immunol Inflamm 2006;14:241–244
   PubMed Web of Science ®Google Scholar
• Efron N, Morgan PB, Makrynioti D. Chronic morbidity of corneal infiltrative events associated with contact lens wear. Cornea 2007;26:793–799
   PubMed Web of Science ®Google Scholar
• Efron N, Al-Dossari M, Pritchard N. Confocal microscopy of the bulbar conjunctiva in contact lens wear. Cornea 2010;29:43–52
   PubMed Web of Science ®Google Scholar
• Arita R, Itoh K, Inoue K, Kuchiba A, Yamaguchi T, Amano S. Contact lens wear is associated with decrease of meibomian glands. Ophthalmology 2009;116:379–384
   PubMed Web of Science ®Google Scholar
• Zhivov A, Stachs O, Stave J, Guthoff RF. In vivo three-dimensional confocal laser scanning microscopy of corneal surface and epithelium. Br J Ophthalmol 2009;93:667–672
   PubMedGoogle Scholar
• Zhivov A, Blum M, Guthoff R, Stachs O. Real-time mapping of the subepithelial nerve plexus by in vivo confocal laser scanning microscopy. Br J Ophthalmol 2010;94:1133–1135
   PubMed Web of Science ®Google Scholar
• Edwards K, Pritchard N, Gosschalk K, Sampson GP, Russell A, Malik RA, et al. Wide-field assessment of the human corneal subbasal nerve plexus in diabetic neuropathy using a novel mapping technique. Cornea 2012;31:1078–1082
   PubMed Web of Science ®Google Scholar
• Petroll WM, Cavanagh HD, Jester JV. Three-dimensional imaging of corneal cells using in vivo confocal microscopy. J Microsc 1993;170:213–219
   PubMed Web of Science ®Google Scholar
• Allgeier S, Zhivov A, Eberle F, Koehler B, Maier S, Bretthauer G, et al. Image reconstruction of the subbasal nerve plexus with in vivo confocal microscopy. Invest Ophthalmol Vis Sci 2011;52:5022–5028
   PubMed Web of Science ®Google Scholar
• Petroll WM, Weaver M, Vaidya S, McCulley JP, Cavanagh HD. Quantitative 3-dimensional corneal imaging in vivo using a modified HRT-RCM confocal microscope. Cornea 2013;32:e36–e43
   PubMedGoogle Scholar
• Zhivov A, Winter K, Peschel S, Guthoff RF, Stachs O, Harder V, et al. [Quantitative analysis of corneal subbasal nerve plexus with in vivo confocal laser scanning microscopy]. Klin Monbl Augenheilkd 2011;228:1067–1072
   PubMed Web of Science ®Google Scholar
• Dabbah MA, Graham J, Petropoulos IN, Tavakoli M, Malik RA. Automatic analysis of diabetic peripheral neuropathy using multi-scale quantitative morphology of nerve fibres in corneal confocal microscopy imaging. Med Image Anal 2011;15:738–747
   PubMed Web of Science ®Google Scholar
• Dabbah MA, Graham J, Petropoulos I, Tavakoli M, Malik RA. Dual-model automatic detection of nerve-fibres in corneal confocal microscopy images. Med Image Comput Comput Assist Interv 2010;13:300–307
   PubMedGoogle Scholar
• Holmes TJ, Pellegrini M, Miller C, Epplin-Zapf T, Larkin S, Luccarelli S, et al. Automated software analysis of corneal micrographs for peripheral neuropathy. Invest Ophthalmol Vis Sci 2010;51:4480–4491
   PubMed Web of Science ®Google Scholar
• Lemp MA, Dilly PN, Boyde A. Tandem-scanning (confocal) microscopy of the full-thickness cornea. Cornea 1985–1986;4:205–209
   PubMedGoogle Scholar