Advanced search
Publication Cover
Current Eye Research Volume 5, 1986 - Issue 1
Submit an article Journal homepage
12
Views
14
CrossRef citations to date
0
Altmetric
Original Article

A new HPLC method to determine glutathione-protein mixed disulfide

Abraham Spector Biochemistry and Molecular Biology Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032, USA
,
Guo-Ming Wang Biochemistry and Molecular Biology Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032, USA
&
Ruey-Ruey C. Huang Biochemistry and Molecular Biology Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032, USA
Pages 47-51 | Received 09 Sep 1985, Accepted 07 Nov 1985, Published online: 02 Jul 2009

References

  • Reed D. J., Babson J. R., Beatty P. W., Brodie A. E., Ellis W. W., Potter D. W. High performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfides and related thiols and disulfides. Anal. Biochem. 1980; 106: 55–62
  • Chaney W. L., Spector A. HPLC analysis of lens GSH and GSSG. Curr. Eye Res. 1984; 3: 345–350
  • Akerboom T. P.M., Sies H. Assay of glutathione, glutathione disulfide and glutathione mixed disulfides in biological samples. Methods in Enzymology 1981; 77: 373–382
  • Paz M. A., Henson E., Rombauer R., Abrash L., Blunenfeld U. O., Gallop P. M. α-amino alcohols as products of a reductive side reaction of denatured collagen with sodium borohydride. Biochemistry 1970; 9: 2123–2127
  • Crestfield A. M., Moore S., Stein W. H. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J. Biol. Chem. 1963; 238: 622–627
  • Anderson E. I., Spector A. The state of sulfhydryl groups in normal and cataractous human lens proteins. I. Nuclear region. Exp. Eye Res. 1978; 26: 404–417
  • Lou M. F., McKeller R., Chyan O. Quantitation of glutathione protein mixed disulfide in lens by ion exchange chromatography. Invest. Ophthalmol. & Vis. Sci. 1985; 26(Suppl.)303
  • Hirs C. H.W. Performic acid oxidation. Methods in Enzymology 1967; XI: 197–199
  • Creighton T. E. Disulfide bond formation in proteins. Methods in Enzymology 1984; 107: 305–329
  • Kesner L., Muntwyler E., Griffin G., Quaranta P. Determination of amino acids as DNP derivitives by automatic partition chromatography. Methods in Enzymology 1967; XI: 94–108
  • Spector A., Huang R. C., Wang G. The effect of H2-O2 on lens epithelial cell glutathione. 1985, submitted for publication

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.