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Current Eye Research Volume 7, 1988 - Issue 2
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Original Article

Preparation of overlapping peptides of bovine retinal S-antigen and their localization by immunoblotting with peptide-specific antibodies

Steven P. Fling Department of Microbiology, University of Minnesota, Box 493 UMHC, Minneapolis, MN, 55455, USA
,
Volker Knospe Department of Ophthalmology, University of Minnesota, Box 493 UMHC, Minneapolis, MN, 55455, USA
&
Dale S. Gregerson Department of Microbiology, University of Minnesota, Box 493 UMHC, Minneapolis, MN, 55455, USA; Department of Ophthalmology, University of Minnesota, Box 493 UMHC, Minneapolis, MN, 55455, USA
Pages 191-199 | Received 19 Oct 1987, Accepted 21 Dec 1987, Published online: 02 Jul 2009

References

  • Gregerson D. S., Putterman G. J. Preparation, isolation, and immunochemical studies of the cyanogen bromide peptides from a retinal photoreceptor cell autoantigen, S-antigen. J. Immunol. 1984; 133: 843–848
  • Gregerson D. S., Fling S. P., Wohlhueter R. M. Characterization of immunologically active cyanogen bromide peptide fragments of bovine and human retinal S-antigen. Exp. Eye Res. 1986a; 43: 803–818
  • Gregerson D. S., Obritsch W. F., Fling S. P., Cameron J. D. S-antigen-specific rat T cell lines recognize peptide fragments of S-antigen and mediate experimental autoimmune uveoretinitis and pinealitis. J. Immunol. 1986b; 136: 2875–2882
  • Gregerson D. S., Obritsch W. F., Fling S. P. Identification of a uveitogenic cyanogen bromide peptide fragment of bovine retinal S-antigen and preparation of a uveitogenic, peptide-specific T celi line. Eur. J. Immunol 1987; 17: 405–411
  • Mahoney W. C., Smith P. K., Hermodson M. A. Fragmentation of proteins with o-iodosobenzoic acid: chemical mechanism and identification of o-iodoxybenzoic acid as a reactive contaminant that modifies tyrosyl residues. Biochemistry 1981; 20: 443–448
  • Landon M. Cleavage at aspartyl-prolyl bonds. Meth. Enzym. 1977; 47: 145–149
  • Knospe V., Fling S. P., Gregerson D. S. Assignment of several epitopes to cyanogen bromide peptides of bovine retinal S-antigen by immunoblotting with peptide-specific antibodies. Curr. Eye Res. 1988; 7: 181–189
  • Wistow G. J., Katial A., Craft C., Shinohara T. Sequence analysis of bovine retinal S-antigen: Relationships with a-transducin and G-proteins. FEBS Lett. 1986; 196: 23–28
  • Yamaki K., Takahashi Y., Sakuragi S., Matsubara K. Molecular cloning of the S-antigen cDNA from bovine retina. Bioc. Biophy. Res. Comm. 1987; 142: 904–910
  • Donoso L. A., Merryman C. F., Sery T. W., Shinohara T., Dietzschold B., Smith A., Kalsow C. M. S-antigen: characterization of a pathogenic epitope which mediates experimental autoimmune uveitis and pinealitis in Lewis rats. Curr. Eye Res. 1987; 6: 1151–1159
  • Donoso L. A., Merryman C. F., Shinohara T., Dietzshold B., Wistow G., Craft C, Morley W., Henry R. T. S-antigen: identification of the MAbA9-C6 monoclonal antibody binding site and the uveitopathogenic sites. Curr. Eye Res. 1986; 5: 995–1004

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