2
Views
29
CrossRef citations to date
0
Altmetric
Original Article

The human lens intrinsic membrane protein MP70 (Cx50) gene: clonal analysis and chromosome mapping

, &
Pages 215-221 | Received 27 Sep 1994, Accepted 16 Nov 1994, Published online: 02 Jul 2009

References

  • Kistler J., Bullivant S. Structural and molecular biology of the eye lens membranes. Crit. Rev. Biochem. Mol. Biol. 1989; 24: 151–181
  • Zampighi G. A., Hall J. E., Ehring G. R., Simon S. A. The structural organization and protein composition of lens fiber junctions. J. Cell Biol. 1989; 108: 2255–2275
  • Gorin M. B., Yancey S. B., Cline J., Revel J. P., Horwitz J. The major intrinsic protein (MIP) of the bovine lens fiber membrane: Characterization and structure based on cDNA cloning. Cell 1984; 39: 49–59
  • Gutekunst K. A., Rao G. N., Church R. L. Molecular cloning and complete nucleotide sequence of the cDNA encoding a bovine lens intrinsic membrane protein (MP19). Curr. Eye Res. 1990; 9: 955–961
  • Pisano M. M., Chepelinsky A. B. Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens. Genomics 1991; 11: 981–990
  • Church R. L., Wang J. H. The human lens fiber-cell intrinsic membrane protein MP19 gene: isolation and sequence analysis. Curr. Eye Res. 1993; 12: 1057–1065
  • Sparkes R. S., Mohandas T., Heinzmann C., Gorin M. B., Horwitz J., Law M. L., Jones C. A., Bateman J. B. The gene for the major intrinsic protein (MIP) of the ocular lens is assigned to human chromosome 12cen-q14. Invest. Ophthalmol. Vis. Sci. 1986; 27: 1351–1354
  • Lieuallen K., Christensen M., Brandriff B., Church R., Wang J. H., Lennon G. Assignment of the human lens fiber cell MP 19 gene (LIM2) to chromosome 19q13.4, and adjacent to ETFB. Somatic Cell Mol. Genet. 1994; 20: 67–69
  • Hsieh C. L., Kumar N. M., Gilula N. B., Francke U. Distribution of genes for gap junction membrane channel proteins on human and mouse chromosomes. Somat. Cell. Mol. Genet. 1991; 17: 91–200
  • Kistler J., Christie D., Bullivant S. Homologies between gap junction proteins in lens, heart and liver. Nature 1988; 331: 721–723
  • Rao G. N., Gutekunst K. A., Church R. L. Bovine lens membrane proteins: MP70, MP64, and MP38 are products of the same gene. Ophthal. Res. 1990; 22: 166–172
  • White T. W., Bruzzone R., Goodenough D. A., Paul D. L. Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. Mol. Biol. Cell. 1992; 3: 711–720
  • Donaldson P., Kistler J. Reconstitution of channels from preparations enriched in lens gap junction protein MP70. J Membr. Biol. 1992; 129: 155–165
  • Paul D. L., Ebihara L., Takemoto L. J., Swenson K. I., Goodenough D. A. Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. J. Cell Biol. 1991; 115: 1077–1089
  • Gruijters W. T. M., Kistler J., Bullivant S., Goodenough D. A. Immunolocalization of MP70 in lens fiber 16–17 nm intercellular junctions. J. Cell Biol. 1987; 104: 565–572
  • Hatae T., Iida H., Kuraoka A., Shibata Y. Cytoplasmic surface ultrastructures of gap junctions in bovine lens fibers. Invest. Ophthalmol. Vis. Sci. 1993; 34: 2164–2173
  • Tenbroek E., Arneson M., Jarvis L., Louis C. The distribution of the fiber cell intrinsic membrane proteins MP20 and connexin46 in the bovine lens. J. Cell-Sci. 1992; 103: 245–257
  • Church R. L., Wang J. H. Assignment of the lens intrinsic membrane protein MP 19 structural gene to human chromosome 19. Current Eye Res. 1992; 11: 421–424
  • Church R. L., Wang J. H., Steele E. Human lens intrinsic membrane protein MP70: Clonal analysis and chromosome mapping. Invest. Ophthal. Vis. Sci. 1994; 35: 2115
  • Church R. L., Wang J. H. The human lens fiber-cell intrinsic membrane protein MP 19 gene: Isolation and sequence analysis. Curr. Eye Res. 1993; 12: 1057–1065
  • Hsiao K. A fast and simple procedure for sequencing double stranded DNA with Sequenase. Nucleic Acids Res. 1991; 19: 2787
  • Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 1982; 157: 105–132
  • Gamier J., Osguthorpe D. J., Robson B. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 1978; 120: 97–120
  • Eisenberg D., Weiss R. M., Terwilliger T. C. The helical hydrophobic moment: a measure of the amphiphilicity of a helix. Nature 1982; 299: 371–374
  • Zimmer D. B., Greem C. R., Evans W. H., Gilula N. B. Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures. J. Biol. Chem. 1987; 262: 7751–7763
  • Van Eldik L. J., Hertzberg E. L., Berdan R. C., Gilula N. B. Interaction of calmodulin and other calcium-modulated proteins with mammalian and arthropod junctional membrane proteins. Biochem. Biophys. Res. Comm. 1985; 126: 825–832
  • Edelman A. M., Blumenthal D. K., Krebs E. G. Protein serine/threonine kinases. Ann. Rev. Biochem. 1987; 56: 567–613
  • Andersen A. S., Pettersson A. F., Kjeldsen T. B. A fast and simple technique for sequencing plasmid DNA with Sequenase using heat denaturation. BioTechniques 1992; 13: 678–679
  • Carlock L. R., Smith D., Wasmuth J. J. Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4. Somatic Cell Mol. Genet. 1986; 12: 163–174

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.