ROCK inhibition enhances aggrecan deposition and suppresses matrix metalloproteinase-3 production in human articular chondrocytes

References

• Heinegård D, Saxne T. The role of the cartilage matrix in osteoarthritis. Nat Rev Rheumatol 2011;7:50–6
   PubMed Web of Science ®Google Scholar
• van den Berg WB. Osteoarthritis year 2010 in review: pathomechanisms. Osteoarthritis Cartilage 2011;19:338–41
   PubMed Web of Science ®Google Scholar
• Shiomi T, Lemaître V, D’Armiento J, Okada Y. Matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs in non-neoplastic diseases. Pathol Int 2010;60:477–96
   PubMed Web of Science ®Google Scholar
• Baillat D, Bègue A, Stéhelin D, Aumercier M. ETS-1 transcription factor binds cooperatively to the palindromic head to head ETS-binding sites of the stromelysin-1 promoter by counteracting autoinhibition. J Biol Chem 2002;277:29386–98
   PubMed Web of Science ®Google Scholar
• Bond M, Baker AH, Newby AC. Nuclear factor κB activity is essential for matrix metalloproteinase-1 and -3 upregulation in rabbit dermal fibroblasts. Biochem Biophys Res Commun 1999;264:561–7
   PubMed Web of Science ®Google Scholar
• Sun HB. CITED2 mechanoregulation of matrix metalloproteinases. Ann N Y Acad Sci 2010;1192:429–36
   PubMed Web of Science ®Google Scholar
• Lin Z, Fitzgerald JB, Xu J, Willers C, Wood D, Grodzinsky AJ, Zheng MH. Gene expression profiles of human chondrocytes during passaged monolayer cultivation. J Orthop Res 2008;26:1230–7
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Asahara H. Histone acetylation influences the activity of Sox9-related transcriptional complex. Acta Med Okayama 2010;64:351–7
   PubMed Web of Science ®Google Scholar
• Han Y, Lefebvre V. L-Sox5 and Sox6 drive expression of the aggrecan gene in cartilage by securing binding of Sox9 to a far-upstream enhancer. Mol Cell Biol 2008;28:4999–5013
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Tsuda M, Taniguchi N, Tajima Y, Asahara H. Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment. J Biol Chem 2005;280:8343–50
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Ozaki T, Asahara H. Smad3 activates the Sox9-dependent transcription on chromatin. Int J Biochem Cell Biol 2009;41:1198–204
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Shukunami C, Amemiya-Kudo M, Shimano H, Ozaki T. Scleraxis and E47 cooperatively regulate the Sox9-dependent transcription. Int J Biochem Cell Biol 2010;42:148–56
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H. Sox9 and p300 cooperatively regulate chromatin-mediated transcription. J Biol Chem 2005;280:35203–8
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Ozaki T. Epigenetic regulation in chondrogenesis. Acta Med Okayama 2010;64:155–61
   PubMed Web of Science ®Google Scholar
• Bonaventure J, Kadhom N, Cohen-Solal L, Ng KH, Bourguignon J, Lasselin C, Freisinger P. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res 1994;212:97–104
   PubMed Web of Science ®Google Scholar
• Stokes DG, Liu G, Dharmavaram R, Hawkins D, Piera-Velazquez S, Jimenez SA. Regulation of type-II collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves SRY-type high-mobility-group box (SOX) transcription factors. Biochem J 2001;360:461–70
   PubMed Web of Science ®Google Scholar
• Takata N, Furumatsu T, Abe N, Naruse K, Ozaki T. Comparison between loose fragment chondrocytes and condyle fibrochondrocytes in cellular proliferation and redifferentiation. J Orthop Sci 2011;16:589–97
   PubMed Web of Science ®Google Scholar
• Sakata K, Furumatsu T, Miyazawa S, Okada Y, Fujii M, Ozaki T. Comparison between normal and loose fragment chondrocytes in proliferation and redifferentiation potential. Int Orthop 2013;37:159–65
   PubMed Web of Science ®Google Scholar
• Matsumoto E, Furumatsu T, Kanazawa T, Tamura M, Ozaki T. ROCK inhibitor prevents the dedifferentiation of human articular chondrocytes. Biochem Biophys Res Commun 2012;420:124–9
   PubMed Web of Science ®Google Scholar
• Nobes CD, Hall A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995;81:53–62
   PubMed Web of Science ®Google Scholar
• Date H, Furumatsu T, Sakoma Y, Yoshida A, Hayashi Y, Abe N, Ozaki T. GDF-5/7 and bFGF activate integrin α2-mediated cellular migration in rabbit ligament fibroblasts. J Orthop Res 2010;28:225–31
   PubMed Web of Science ®Google Scholar
• Saiga K, Furumatsu F, Yoshida A, Masuda S, Takihira S, Abe N, Ozaki T. Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury. Biochem Biophys Res Commun 2010;402:329–34
   PubMed Web of Science ®Google Scholar
• Miyake Y, Furumatsu T, Kubota S, Kawata K, Ozaki T, Takigawa M. Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells. Biochem Biophys Res Commun 2011;409:247–52
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Kanazawa T, Yokoyama Y, Abe N, Ozaki T. Inner meniscus cells maintain higher chondrogenic phenotype compared with outer meniscus cells. Connect Tissue Res 2011;52:459–65
   PubMed Web of Science ®Google Scholar
• Tetsunaga T, Furumatsu T, Abe N, Nishida K, Naruse K, Ozaki T. Mechanical stretch stimulates integrin αVβ3-mediated collagen expression in human anterior cruciate ligament cells. J Biomech 2009;42:2097–103
   PubMed Web of Science ®Google Scholar
• Kanazawa T, Furumatsu T, Hachioji M, Oohashi T, Ninomiya Y, Ozaki T. Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells. J Orthop Res 2012;30:468–74
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Hachioji M, Saiga K, Takata N, Yokoyama Y, Ozaki T. Anterior cruciate ligament-derived cells have high chondrogenic potential. Biochem Biophys Res Commun 2010;391:1142–7
   PubMed Web of Science ®Google Scholar
• Fujii M, Furumatsu T, Yokoyama Y, Kanazawa T, Kajiki Y, Abe N, Ozaki T. Chondromodulin-I derived from the inner meniscus prevents endothelial cell proliferation. J Orthop Res 2013;31:538–43
   PubMed Web of Science ®Google Scholar
• Furumatsu T, Matsumoto E, Kanazawa T, Fujii M, Lu Z, Kajiki R, Ozaki T. Tensile strain increases expression of CCN2 and COL2A1 by activating TGF-β-Smad2/3 pathway in chondrocytic cells. J Biomech 2013;46:1508–15
   PubMed Web of Science ®Google Scholar
• Woods A, Wang G, Beier F. RhoA/ROCK signaling regulates Sox9 expression and actin organization during chondrogenesis. J Biol Chem 2005;280:11626–34
   PubMed Web of Science ®Google Scholar
• Woods A, Beier F. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem 2006;281:13134–40
   PubMed Web of Science ®Google Scholar
• Haudenschild DR, Chen J, Pang N, Lotz MK, D'Lima DD. Rho kinase-dependent activation of SOX9 in chondrocytes. Arthritis Rheum 2010;62:191–200
   PubMed Web of Science ®Google Scholar
• Kumar D, Lassar AB. The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol Cell Biol 2009;29:4262–73
   PubMed Web of Science ®Google Scholar
• Appleton CT, Usmani SE, Mort JS, Beier F. Rho/ROCK and MEK/ERK activation by transforming growth factor-α induces articular cartilage degradation. Lab Invest 2010;90:20–30
   PubMed Web of Science ®Google Scholar
• Das RH, Jahr H, Verhaar JA, van der Linden JC, van Osch GJ, Weinans H. In vitro expansion affects the response of chondrocytes to mechanical stimulation. Osteoarthritis Cartilage 2008;16:385–91
   PubMed Web of Science ®Google Scholar
• Peng H, Tan L, Osaki M, Zhan Y, Ijiri K, Tsuchimochi K, Otero M, Wang H, Choy BK, Grall FT, Gu X, Libermann TA, Oettgen P, Goldring MB. ESE-1 is a potent repressor of type II collagen gene (COL2A1) transcription in human chondrocytes. J Cell Physiol 2008;215:562–73
   PubMed Web of Science ®Google Scholar
• Takeshita N, Yoshimi E, Hatori C, Kumakura F, Seki N, Shimizu Y. Alleviating effects of AS1892802, a Rho kinase inhibitor, on osteoarthritic disorders in rodents. J Pharmacol Sci 2011;115:481–9
   PubMed Web of Science ®Google Scholar