The applications of buckminsterfullerene C₆₀ and derivatives in orthopaedic research

References

• Aschberger K, Johnston HJ, Stone V, Aitken RJ, Tran CL, Hankin SM, Peters SA, Christensen FM. Review of fullerene toxicity and exposure – appraisal of a human health risk assessment, based on open literature. Regul Toxicol Pharmacol 2010;58:455–73
   PubMed Web of Science ®Google Scholar
• Kroto HW, Heath JR, Obrien SC, Curl RF, Smalley RE. C-60 – Buckminsterfullerene. Nature 1985;318:162–3
   Web of Science ®Google Scholar
• Satoh M, Takayanagi I. Pharmacological studies on fullerene (C60), a novel carbon allotrope, and its derivatives. J Pharmacol Sci 2006;100:513–18
   PubMed Web of Science ®Google Scholar
• Krusic PJ, Wasserman E, Keizer PN, Morton JR, Preston KF. Radical reactions of c60. Science 1991;254:1183–5
   PubMed Web of Science ®Google Scholar
• Nielsen GD, Roursgaard M, Jensen KA, Poulsen SS, Larsen ST. In vivo biology and toxicology of fullerenes and their derivatives. Basic Clin Pharmacol Toxicol 2008;103:197–208
   PubMed Web of Science ®Google Scholar
• Bosi S, Da Ros T, Spalluto G, Prato M. Fullerene derivatives: an attractive tool for biological applications. Euro J Med Chem 2003;38:913–23
   PubMed Web of Science ®Google Scholar
• Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 2012;24:981–90
   PubMed Web of Science ®Google Scholar
• Perry G, Raina AK, Nunomura A, Wataya T, Sayre LM, Smith MA. How important is oxidative damage? Lessons from Alzheimer's disease. Free Radic Biol Med 2000;28:831–4
   PubMed Web of Science ®Google Scholar
• Ding Z, Liu S, Wang X, Khaidakov M, Dai Y, Mehta JL. Oxidant stress in mitochondrial DNA damage, autophagy and inflammation in atherosclerosis. Sci Rep 2013;3:1077 . doi: 10.1038/srep01077
   PubMedGoogle Scholar
• Andersen JK. Oxidative stress in neurodegeneration: cause or consequence? Nat Med 2004;10 Suppl:S18–25
   PubMed Web of Science ®Google Scholar
• Trachootham D, Alexandre J, Huang P. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 2009;8:579–91
   PubMed Web of Science ®Google Scholar
• Yudoh K, Shishido K, Murayama H, Yano M, Matsubayashi K, Takada H, Nakamura H, Masuko K, Kato T, Nishioka K. Water-soluble C60 fullerene prevents degeneration of articular cartilage in osteoarthritis via down-regulation of chondrocyte catabolic activity and inhibition of cartilage degeneration during disease development. Arthritis Rheum 2007;56:3307–18
   PubMed Web of Science ®Google Scholar
• Yudoh K, Karasawa R, Masuko K, Kato T. Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis. Int J Nanomed 2009;4:233–9
   PubMed Web of Science ®Google Scholar
• Bakry R, Vallant RM, Najam-ul-Haq M, Rainer M, Szabo Z, Huck CW, Bonn GK. Medicinal applications of fullerenes. Int J Nanomed 2007;2:639–49
   PubMed Web of Science ®Google Scholar
• Nakamura E, Isobe H. Functionalized fullerenes in water. The first 10 years of their chemistry, biology, and nanoscience. Acc Chem Res 2003;36:807–15
   PubMed Web of Science ®Google Scholar
• Xiao L, Aoshima H, Saitoh Y, Miwa N. Highly hydroxylated fullerene localizes at the cytoskeleton and inhibits oxidative stress in adipocytes and a subcutaneous adipose-tissue equivalent. Free Radic Biol Med 2011;51:1376–89
   PubMed Web of Science ®Google Scholar
• Chistyakov VA, Smirnova YO, Prazdnova EV, Soldatov AV. Possible mechanisms of fullerene C60 antioxidant action. BioMed Res Int 2013;2013:821498
   PubMed Web of Science ®Google Scholar
• Andrievsky GV, Bruskov VI, Tykhomyrov AA, Gudkov SV. Peculiarities of the antioxidant and radioprotective effects of hydrated C60 fullerene nanostuctures in vitro and in vivo. Free Radic Biol Med 2009;47:786–93
   PubMed Web of Science ®Google Scholar
• Roursgaard M, Poulsen SS, Kepley CL, Hammer M, Nielsen GD, Larsen ST. Polyhydroxylated C60 fullerene (fullerenol) attenuates neutrophilic lung inflammation in mice. Basic Clin Pharmacol Toxicol 2008;103:386–8
   PubMed Web of Science ®Google Scholar
• Huang ST, Liao JS, Fang HW, Lin CM. Synthesis and anti-inflammation evaluation of new C60 fulleropyrrolidines bearing biologically active xanthine. Bioorg Med Chem Lett 2008;18:99–103
   PubMed Web of Science ®Google Scholar
• Huang ST, Ho CS, Lin CM, Fang HW, Peng YX. Development and biological evaluation of C(60) fulleropyrrolidine-thalidomide dyad as a new anti-inflammation agent. Bioorg Med Chem 2008;16:8619–26
   PubMed Web of Science ®Google Scholar
• Makarova EG, Gordon RY, Podolski IY. Fullerene C60 prevents neurotoxicity induced by intrahippocampal microinjection of amyloid-beta peptide. J Nanosci Nanotechnol 2012;12:119–26
   PubMed Web of Science ®Google Scholar
• Dugan LL, Gabrielsen JK, Yu SP, Lin TS, Choi DW. Buckminsterfullerenol free radical scavengers reduce excitotoxic and apoptotic death of cultured cortical neurons. Neurobiol Disease 1996;3:129–35
   PubMed Web of Science ®Google Scholar
• Dugan LL, Turetsky DM, Du C, Lobner D, Wheeler M, Almli CR, Shen CK, Luh TY, Choi DW, Lin TS. Carboxyfullerenes as neuroprotective agents. Proc Natl Acad Sci USA 1997;94:9434–9
   PubMed Web of Science ®Google Scholar
• Lin AM, Fang SF, Lin SZ, Chou CK, Luh TY, Ho LT. Local carboxyfullerene protects cortical infarction in rat brain. Neurosci Res 2002;43:317–21
   PubMed Web of Science ®Google Scholar
• Lin AM, Chyi BY, Wang SD, Yu HH, Kanakamma PP, Luh TY, Chou CK, Ho LT. Carboxyfullerene prevents iron-induced oxidative stress in rat brain. J Neurochem 1999;72:1634–40
   PubMed Web of Science ®Google Scholar
• Gharbi N, Pressac M, Hadchouel M, Szwarc H, Wilson SR, Moussa F. [60]fullerene is a powerful antioxidant in vivo with no acute or subacute toxicity. Nano Lett 2005;5:2578–85
   PubMed Web of Science ®Google Scholar
• Srdjenovic B, Milic-Torres V, Grujic N, Stankov K, Djordjevic A, Vasovic V. Antioxidant properties of fullerenol C60(OH)24 in rat kidneys, testes, and lungs treated with doxorubicin. Toxicol Mech Meth 2010;20:298–305
   PubMed Web of Science ®Google Scholar
• Chueh SC, Lai MK, Lee MS, Chiang LY, Ho TI, Chen SC. Decrease of free radical level in organ perfusate by a novel water-soluble carbon-sixty, hexa(sulfobutyl)fullerenes. Transplant Proc 1999;31:1976–7
   PubMed Web of Science ®Google Scholar
• Hu Z, Zhang C, Tang P, Li C, Yao Y, Sun S, Zhang L, Huang Y. Protection of cells from nitric oxide-mediated apoptotic death by glutathione C(6)(0) derivative. Cell Biol Int 2012;36:677–81
   PubMed Web of Science ®Google Scholar
• Huang YL, Shen CK, Luh TY, Yang HC, Hwang KC, Chou CK. Blockage of apoptotic signaling of transforming growth factor-beta in human hepatoma cells by carboxyfullerene. Euro J Biochem/FEBS 1998;254:38–43
   PubMedGoogle Scholar
• Chien CT, Lee PH, Chen CF, Ma MC, Lai MK, Hsu SM. De novo demonstration and co-localization of free-radical production and apoptosis formation in rat kidney subjected to ischemia/reperfusion. J Am Soc Nephrol: JASN 2001;12:973–82
   PubMed Web of Science ®Google Scholar
• Straface E, Natalini B, Monti D, Franceschi C, Schettini G, Bisaglia M, Fumelli C, Pincelli C, Pellicciari R, Malorni W. C3-fullero-tris-methanodicarboxylic acid protects epithelial cells from radiation-induced anoikia by influencing cell adhesion ability. FEBS Lett 1999;454:335–40
   PubMed Web of Science ®Google Scholar
• Bisaglia M, Natalini B, Pellicciari R, Straface E, Malorni W, Monti D, Franceschi C, Schettini G. C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. J Neurochem 2000;74:1197–204
   PubMed Web of Science ®Google Scholar
• Zhao Q, Li Y, Xu J, Liu R, Li W. Radioprotection by fullerenols of Stylonychia mytilus exposed to gamma-rays. Int J Radiat Biol 2005;81:169–75
   PubMed Web of Science ®Google Scholar
• Theriot CA, Casey RC, Moore VC, Mitchell L, Reynolds JO, Burgoyne M, Partha R, Huff JL, Conyers JL, Jeevarajan A, Wu H. Dendro[C(60)]fullerene DF-1 provides radioprotection to radiosensitive mammalian cells. Radiat Environ Biophys 2010;49:437–45
   PubMed Web of Science ®Google Scholar
• Trajkovic S, Dobric S, Djordjevic A, Dragojevic-Simic V, Milovanovic Z. Radioprotective efficiency of fullerenol in irradiated mice. Mater Sci Forum 2005;494:549–54
   Google Scholar
• Trajkovic S, Dobric S, Jacevic V, Dragojevic-Simic V, Milovanovic Z, Dordevic A. Tissue-protective effects of fullerenol C60(OH)24 and amifostine in irradiated rats. Colloids Surf B, Biointerfaces 2007;58:39–43
   PubMed Web of Science ®Google Scholar
• Meng X, Li B, Chen Z, Yao L, Zhao D, Yang X, He M, Yu Q. Inhibition of a thermophilic deoxyribonucleic acid polymerase by fullerene derivatives. J Enzyme Inhib Med Chem 2007;22:293–6
   PubMed Web of Science ®Google Scholar
• Wolff DJ, Papoiu AD, Mialkowski K, Richardson CF, Schuster DI, Wilson SR. Inhibition of nitric oxide synthase isoforms by tris-malonyl-C(60)-fullerene adducts. Arch Biochem Biophys 2000;378:216–23
   PubMed Web of Science ®Google Scholar
• Ueng TH, Kang JJ, Wang HW, Cheng YW, Chiang LY. Suppression of microsomal cytochrome P450-dependent monooxygenases and mitochondrial oxidative phosphorylation by fullerenol, a polyhydroxylated fullerene C60. Toxicol Lett 1997;93:29–37
   PubMed Web of Science ®Google Scholar
• Liao F, Saitoh Y, Miwa N. Anticancer effects of fullerene [C60] included in polyethylene glycol combined with visible light irradiation through ROS generation and DNA fragmentation on fibrosarcoma cells with scarce cytotoxicity to normal fibroblasts. Oncol Res 2011;19:203–16
   PubMed Web of Science ®Google Scholar
• Mroz P, Pawlak A, Satti M, Lee H, Wharton T, Gali H, Sarna T, Hamblin MR. Functionalized fullerenes mediate photodynamic killing of cancer cells: Type I versus Type II photochemical mechanism. Free Radic Biol Med 2007;43:711–19
   PubMed Web of Science ®Google Scholar
• Hu Z, Zhang C, Huang Y, Sun S, Guan W, Yao Y. Photodynamic anticancer activities of water-soluble C(60) derivatives and their biological consequences in a HeLa cell line. Chem-Biol Interact 2012;195:86–94
   PubMed Web of Science ®Google Scholar
• Brunet L, Lyon DY, Hotze EM, Alvarez PJ, Wiesner MR. Comparative photoactivity and antibacterial properties of C60 fullerenes and titanium dioxide nanoparticles. Environ Sci Technol 2009;43:4355–60
   PubMed Web of Science ®Google Scholar
• Lee I, Mackeyev Y, Cho M, Li D, Kim JH, Wilson LJ, Alvarez PJ. Photochemical and antimicrobial properties of novel C60 derivatives in aqueous systems. Environ Sci Technol 2009;43:6604–10
   PubMed Web of Science ®Google Scholar
• Kasermann F, Kempf C. Buckminsterfullerene and photodynamic inactivation of viruses. Rev Med Virol 1998;8:143–51
   PubMed Web of Science ®Google Scholar
• Rud Y, Buchatskyy L, Prylutskyy Y, Marchenko O, Senenko A, Schutze C, Ritter U. Using C60 fullerenes for photodynamic inactivation of mosquito iridescent viruses. J Enzyme Inhib Med Chem 2012;27:614–17
   PubMed Web of Science ®Google Scholar
• Zakharian TY, Seryshev A, Sitharaman B, Gilbert BE, Knight V, Wilson LJ. A fullerene-paclitaxel chemotherapeutic: synthesis, characterization, and study of biological activity in tissue culture. J Am Chem Soc 2005;127:12508–9
   PubMed Web of Science ®Google Scholar
• Shi J, Zhang H, Wang L, Li L, Wang H, Wang Z, Li Z, Chen C, Hou L, Zhang C, Zhang Z. PEI-derivatized fullerene drug delivery using folate as a homing device targeting to tumor. Biomaterials 2013;34:251–61
   PubMed Web of Science ®Google Scholar
• Sitharaman B, Zakharian TY, Saraf A, Misra P, Ashcroft J, Pan S, Pham QP, Mikos AG, Wilson LJ, Engler DA. Water-soluble fullerene (C60) derivatives as nonviral gene-delivery vectors. Mol Pharm 2008;5:567–78
   PubMed Web of Science ®Google Scholar
• Isobe H, Nakanishi W, Tomita N, Jinno S, Okayama H, Nakamura E. Nonviral gene delivery by tetraamino fullerene. Mol Pharm 2006;3:124–34
   PubMed Web of Science ®Google Scholar
• Anderson SA, Lee KK, Frank JA. Gadolinium-fullerenol as a paramagnetic contrast agent for cellular imaging. Invest Radiol 2006;41:332–8
   PubMed Web of Science ®Google Scholar
• Sitharaman B, Tran LA, Pham QP, Bolskar RD, Muthupillai R, Flamm SD, Mikos AG, Wilson LJ. Gadofullerenes as nanoscale magnetic labels for cellular MRI. Contrast Media Mol Imaging 2007;2:139–46
   PubMed Web of Science ®Google Scholar
• Cagle DW, Kennel SJ, Mirzadeh S, Alford JM, Wilson LJ. In vivo studies of fullerene-based materials using endohedral metallofullerene radiotracers. Proc Natl Acad Sci USA 1999;96:5182–7
   PubMed Web of Science ®Google Scholar
• Qingnuan L, Yan X, Xiaodong Z, Ruili L, Qieqie D, Xiaoguang S, Shaoliang C, Wenxin L. Preparation of (99m)Tc-C(60)(OH)(x) and its biodistribution studies. Nucl Med Biol 2002;29:707–10
   PubMed Web of Science ®Google Scholar
• Tsuchiya T, Yamakoshi YN, Miyata N. A novel promoting action of fullerene C60 on the chondrogenesis in rat embryonic limb bud cell culture system. Biochem Biophys Res Commun 1995;206:885–94
   PubMed Web of Science ®Google Scholar
• Horwitz AL, Dorfman A. Subcellular sites for synthesis of chondromucoprotein of cartilage. J Cell Biol 1968;38:358–68
   PubMed Web of Science ®Google Scholar
• Hungerbuhler H, Guldi DM, Asmus KD. Incorporation of C-60 into Artificial Lipid-Membranes. J Am Chem Soc 1993;115:3386–7
   Web of Science ®Google Scholar
• Nevo Z, Dorfman A. Stimulation of chondromucoprotein synthesis in chondrocytes by extracellular chondromucoprotein. Proc Natl Acad Sci USA 1972;69:2069–72
   PubMed Web of Science ®Google Scholar
• Thannickal VJ, Fanburg BL. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 2000;279:L1005–28
   PubMed Web of Science ®Google Scholar
• Bonizzi G, Piette J, Merville MP, Bours V. Cell type-specific role for reactive oxygen species in nuclear factor-kappaB activation by interleukin-1. Biochem Pharmacol 2000;59:7–11
   PubMed Web of Science ®Google Scholar
• Williams MS, Kwon J. T cell receptor stimulation, reactive oxygen species, and cell signaling. Free Radic Biol Med 2004;37:1144–51
   PubMed Web of Science ®Google Scholar
• Droge W. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47–95
   PubMed Web of Science ®Google Scholar
• Miura K, Kamiya S, Sasaki N. C60 molecular bearings. Phys Rev Lett 2003;90:055509
   Google Scholar
• Mikami T, Ichiseki T, Kaneuji A, Ueda Y, Sugimori T, Fukui K, Matsumoto T. Prevention of steroid-induced osteonecrosis by intravenous administration of vitamin E in a rabbit model. J Orthop Sci 2010;15:674–7
   PubMed Web of Science ®Google Scholar
• Liu H, Yang X, Zhang Y, Dighe A, Li X, Cui Q. Fullerol antagonizes dexamethasone-induced oxidative stress and adipogenesis while enhancing osteogenesis in a cloned bone marrow mesenchymal stem cell. J Orthop Res 2012;30:1051–7
   PubMed Web of Science ®Google Scholar
• Cho SW, Sun HJ, Yang JY, Jung JY, An JH, Cho HY, Choi HJ, Kim SW, Kim SY, Kim D, Shin CS. Transplantation of mesenchymal stem cells overexpressing RANK-Fc or CXCR4 prevents bone loss in ovariectomized mice. Mol Ther 2009;17:1979–87
   Google Scholar
• Wilson ALMaLJ. Functionalization of C60 with diphosphonate groups: a route to bone-vectored fullerenes. J Chem Soc Perkin Trans 2 2002;6:1173–6
   Google Scholar
• Gonzalez KA, Wilson LJ, Wu W, Nancollas GH. Synthesis and in vitro characterization of a tissue-selective fullerene: vectoring C(60)(OH)(16)AMBP to mineralized bone. Bioorg Med Chem 2002;10:1991–7
   PubMed Web of Science ®Google Scholar
• Freemont AJ. The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. Rheumatol (Oxford) 2009;48:5–10
   PubMed Web of Science ®Google Scholar
• Risbud MV, Albert TJ, Guttapalli A, Vresilovic EJ, Hillibrand AS, Vaccaro AR, Shapiro IM. Differentiation of mesenchymal stem cells towards a nucleus pulposus-like phenotype in vitro: implications for cell-based transplantation therapy. Spine (Phila Pa 1976) 2004;29:2627–32
   PubMed Web of Science ®Google Scholar
• Andersson GB. Epidemiological features of chronic low-back pain. Lancet 1999;354:581–5
   PubMed Web of Science ®Google Scholar
• Deyo RA, Weinstein JN. Low back pain. New Eng J Med 2001;344:363–70
   PubMed Web of Science ®Google Scholar
• Nasto LA, Robinson AR, Ngo K, Clauson CL, Dong Q, St Croix C, Sowa G, Pola E, Robbins PD, Kang J, Niedernhofer LJ, Wipf P, Vo NV. Mitochondrial-derived reactive oxygen species (ROS) play a causal role in aging-related intervertebral disc degeneration. J Orthop Res 2013;31:1150--7
   PubMed Web of Science ®Google Scholar
• Ahn SH, Cho YW, Ahn MW, Jang SH, Sohn YK, Kim HS. mRNA expression of cytokines and chemokines in herniated lumbar intervertebral discs. Spine (Phila Pa 1976) 2002;27:911–17
   PubMed Web of Science ®Google Scholar
• Le Maitre CL, Hoyland JA, Freemont AJ. Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1β and TNFα expression profile. Arthritis Res Ther 2007;9:R77 . doi:10.1186/ar2275
   PubMed Web of Science ®Google Scholar
• Bachmeier BE, Nerlich AG, Weiler C, Paesold G, Jochum M, Boos N. Analysis of tissue distribution of TNF-alpha, TNF-alpha-receptors, and the activating TNF-alpha-converting enzyme suggests activation of the TNF-alpha system in the aging intervertebral disc. Ann New York Acad Sci 2007;1096:44–54
   PubMed Web of Science ®Google Scholar
• Moore RJ. The vertebral endplate: disc degeneration, disc regeneration. Euro Spine J 2006;15 Suppl 3:S333–7
   PubMed Web of Science ®Google Scholar
• Modic MT, Masaryk TJ, Ross JS, Carter JR. Imaging of degenerative disk disease. Radiology 1988;168:177–86
   PubMed Web of Science ®Google Scholar
• Wang Y, Battie MC, Boyd SK, Videman T. The osseous endplates in lumbar vertebrae: thickness, bone mineral density and their associations with age and disk degeneration. Bone 2011;48:804–9
   PubMed Web of Science ®Google Scholar
• de Roos A, Kressel H, Spritzer C, Dalinka M. MR imaging of marrow changes adjacent to end plates in degenerative lumbar disk disease. AJR Am J Roentgenol 1987;149:531–4
   PubMed Web of Science ®Google Scholar
• Wang GJ, Sweet DE, Reger SI, Thompson RC. Fat-cell changes as a mechanism of avascular necrosis of the femoral head in cortisone-treated rabbits. J Bone Joint Surg Am 1977;59:729–35
   PubMed Web of Science ®Google Scholar
• Drescher W, Li H, Qvesel D, Jensen SD, Flo C, Hansen ES, Bunger C. Vertebral blood flow and bone mineral density during long-term corticosteroid treatment: an experimental study in immature pigs. Spine (Phila Pa 1976) 2000;25:3021–5
   PubMed Web of Science ®Google Scholar
• Liu Q, Jin L, Shen FH, Balian G, Li XJ. Fullerol nanoparticles suppress inflammatory response and adipogenesis of vertebral bone marrow stromal cells-a potential novel treatment for intervertebral disc degeneration. Spine J 2013;13:1571--80
   PubMed Web of Science ®Google Scholar
• Yamago S, Tokuyama H, Nakamura E, Kikuchi K, Kananishi S, Sueki K, Nakahara H, Enomoto S, Ambe F. In vivo biological behavior of a water-miscible fullerene: 14C labeling, absorption, distribution, excretion and acute toxicity. Chem Biol 1995;2:385–9
   PubMed Web of Science ®Google Scholar
• Lee M, Kim BJ, Lim EJ, Back SK, Lee JH, Yu SW, Hong SH, Kim JH, Lee SH, Jung WW, Sul D, Na HS. Complete Freund's adjuvant-induced intervertebral discitis as an animal model for discogenic low back pain. Anesth Analg 2009;109:1287–96
   PubMed Web of Science ®Google Scholar
• Moon HJ, Kim JH, Lee HS, Chotai S, Kang JD, Suh JK, Park YK. Annulus fibrosus cells interact with neuron-like cells to modulate production of growth factors and cytokines in symptomatic disc degeneration. Spine (Phila Pa 1976) 2012;37:2–9
   PubMed Web of Science ®Google Scholar
• Hadjipavlou AG, Simmons JW, Yang JP, Bi LX, Simmons DJ, Necessary JT. Torsional injury resulting in disc degeneration in the rabbit: II. Associative changes in dorsal root ganglion and spinal cord neurotransmitter production. J Spinal Disord 1998;11:318–21
   Google Scholar
• Liu Q, Jin L, Mahon BH, Chordia MD, Shen FH, Li X. A novel treatment of neuroinflammation against low back pain by soluble fullerol nanoparticles. Spine (Phila Pa 1976) 2013;38:1443--51
   PubMed Web of Science ®Google Scholar
• Huang ST, Ho CS, Lin CM, Fang HW, Peng YX. Development and biological evaluation of C(60) fulleropyrrolidine-thalidomide dyad as a new anti-inflammation agent. Bioorg Med Chem 2008;16:8619–26
   PubMed Web of Science ®Google Scholar
• Roberts JE, Wielgus AR, Boyes WK, Andley U, Chignell CF. Phototoxicity and cytotoxicity of fullerol in human lens epithelial cells. Toxicol Appl Pharmacol 2008;228:49–58
   PubMed Web of Science ®Google Scholar
• Henry TB, Petersen EJ, Compton RN. Aqueous fullerene aggregates (nC60) generate minimal reactive oxygen species and are of low toxicity in fish: a revision of previous reports. Curr Opin Biotechnol 2011;22:533–7
   PubMed Web of Science ®Google Scholar
• Oberdorster E. Manufactured nanomaterials (fullerenes, C60) induce oxidative stress in the brain of juvenile largemouth bass. Environ Health Perspect 2004;112:1058–62
   PubMed Web of Science ®Google Scholar
• Sayes CM, Gobin AM, Ausman KD, Mendez J, West JL, Colvin VL. Nano-C60 cytotoxicity is due to lipid peroxidation. Biomaterials 2005;26:7587–95
   PubMed Web of Science ®Google Scholar
• Zhang B, Cho M, Fortner JD, Lee J, Huang CH, Hughes JB, Kim JH. Delineating oxidative processes of aqueous C60 preparations: role of THF peroxide. Environ Sci Technol 2009;43:108–13
   PubMed Web of Science ®Google Scholar
• Sayes CM, Fortner JD, Guo W, Lyon D, Boyd AM, Ausman KD, Tao YJ, Sitharaman B, Wilson LJ, Hughes JB, West JL, Colvin VL. The differential cytotoxicity of water-soluble fullerenes. Nano Lett 2004;4:1881–7
   Web of Science ®Google Scholar
• Yamawaki H, Iwai N. Cytotoxicity of water-soluble fullerene in vascular endothelial cells. Am J Physiol Cell Physiol 2006;290:C1495–502
   PubMed Web of Science ®Google Scholar
• Mori T, Takada H, Ito S, Matsubayashi K, Miwa N, Sawaguchi T. Preclinical studies on safety of fullerene upon acute oral administration and evaluation for no mutagenesis. Toxicology 2006;225:48–54
   PubMed Web of Science ®Google Scholar
• Nelson MA, Domann FE, Bowden GT, Hooser SB, Fernando Q, Carter DE. Effects of acute and subchronic exposure of topically applied fullerene extracts on the mouse skin. Toxicol Industrial Health 1993;9:623–30
   PubMed Web of Science ®Google Scholar
• Cray J, Jr Henderson SE, Smith DM, Kinsella CR Jr, Bykowski M, Cooper GM, Almarza AJ, Losee JE. BMP-2-regenerated calvarial bone: a biomechanical appraisal in a large animal model. Ann Plastic Surgery 2013 . [Epub ahead of print]
   Web of Science ®Google Scholar
• Struewer J, Cronlein M, Ziring E, Schwarting T, Kratz M, Ruchholtz S, Frangen TM. Effect of bone morphogenetic protein-2 on tendon-bone integration in an in vitro cell culture. Orthopedics 2013;36:e200–6
   PubMed Web of Science ®Google Scholar
• Cordonnier T, Langonne A, Sohier J, Layrolle P, Rosset P, Sensebe L, Deschaseaux F. Consistent osteoblastic differentiation of human mesenchymal stem cells with bone morphogenetic protein 4 and low serum. Tissue Eng Part C, Meth 2011;17:249–59
   PubMed Web of Science ®Google Scholar
• Shiozaki Y, Kitajima T, Mazaki T, Yoshida A, Tanaka M, Umezawa A, Nakamura M, Yoshida Y, Ito Y, Ozaki T, Matsukawa A. Enhanced in vivo osteogenesis by nanocarrier-fused bone morphogenetic protein-4. Int J Nanomed 2013;8:1349–60
   PubMed Web of Science ®Google Scholar
• Vukicevic S, Grgurevic L. BMP-6 and mesenchymal stem cell differentiation. Cytokine Growth Factor Rev 2009;20:441–8
   PubMed Web of Science ®Google Scholar
• Grasser WA, Orlic I, Borovecki F, Riccardi KA, Simic P, Vukicevic S, Paralkar VM. BMP-6 exerts its osteoinductive effect through activation of IGF-I and EGF pathways. Int Orthop 2007;31:759–65
   PubMed Web of Science ®Google Scholar
• Feng G, Wan Y, Balian G, Laurencin CT, Li X. Adenovirus-mediated expression of growth and differentiation factor-5 promotes chondrogenesis of adipose stem cells. Growth Factors 2008;26:132–42
   PubMed Web of Science ®Google Scholar
• Cui M, Wan Y, Anderson DG, Shen FH, Leo BM, Laurencin CT, Balian G, Li X. Mouse growth and differentiation factor-5 protein and DNA therapy potentiates intervertebral disc cell aggregation and chondrogenic gene expression. Spine J 2008;8:287–95
   PubMed Web of Science ®Google Scholar
• Thompson JP, Oegema TR Jr, Bradford DS. Stimulation of mature canine intervertebral disc by growth factors. Spine (Phila Pa 1976) 1991;16:253–60
   PubMed Web of Science ®Google Scholar
• Gruber HE, Fisher EC Jr, Desai B, Stasky AA, Hoelscher G, Hanley EN Jr. Human intervertebral disc cells from the annulus: three-dimensional culture in agarose or alginate and responsiveness to TGF-beta1. Exp Cell Res 1997;235:13–21
   PubMed Web of Science ®Google Scholar
• Matsunaga S, Nagano S, Onishi T, Morimoto N, Suzuki S, Komiya S. Age-related changes in expression of transforming growth factor-beta and receptors in cells of intervertebral discs. J Neurosurg 2003;98:63–7
   PubMed Web of Science ®Google Scholar
• Specchia N, Pagnotta A, Toesca A, Greco F. Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine. Euro Spine J 2002;11:145–51
   PubMed Web of Science ®Google Scholar
• Okuda S, Myoui A, Ariga K, Nakase T, Yonenobu K, Yoshikawa H. Mechanisms of age-related decline in insulin-like growth factor-I dependent proteoglycan synthesis in rat intervertebral disc cells. Spine (Phila Pa 1976) 2001;26:2421–6
   PubMed Web of Science ®Google Scholar
• Osada R, Ohshima H, Ishihara H, Yudoh K, Sakai K, Matsui H, Tsuji H. Autocrine/paracrine mechanism of insulin-like growth factor-1 secretion, and the effect of insulin-like growth factor-1 on proteoglycan synthesis in bovine intervertebral discs. J Orthop Res 1996;14:690–9
   PubMed Web of Science ®Google Scholar
• Gruber HE, Norton HJ, Hanley EN Jr. Anti-apoptotic effects of IGF-1 and PDGF on human intervertebral disc cells in vitro. Spine (Phila Pa 1976) 2000;25:2153–7
   PubMed Web of Science ®Google Scholar
• Bobacz K, Gruber R, Soleiman A, Graninger WB, Luyten FP, Erlacher L. Cartilage-derived morphogenetic protein-1 and -2 are endogenously expressed in healthy and osteoarthritic human articular chondrocytes and stimulate matrix synthesis. Osteoarthritis Cartilage 2002;10:394–401
   PubMed Web of Science ®Google Scholar
• Yang J, Alemany LB, Driver J, Hartgerink JD, Barron AR. Fullerene-derivatized amino acids: synthesis, characterization, antioxidant properties, and solid-phase peptide synthesis. Chemistry 2007;13:2530–45
   PubMed Web of Science ®Google Scholar
• Da Ros T, Bergamin M, Vazquez E, Spalluto G, Baiti B, Moro S, Boutorine A, Prato M. Synthesis and molecular modeling studies of fullerene-5,6,7-trimethoxyindole-oligonucleotide conjugates as possible probes for study of photochemical reactions in DNA triple helices. Eur J Org Chem 2002;3:405–13
   Google Scholar
• Imahori H, Fukuzumi S. Porphyrin- and fullerene-based molecular photovoltaic devices. Adv Funct Mater 2004;14:525–36
   Web of Science ®Google Scholar
• de la Torre MDL, Rodrigues AGP, Tome AC, Silva AMS, Cavaleiro JAS. [60]Fullerene-flavonoid dyads. Tetrahedron 2004;60:3581–92
   Web of Science ®Google Scholar
• Shi J, Votruba AR, Farokhzad OC, Langer R. Nanotechnology in drug delivery and tissue engineering: from discovery to applications. Nano Lett 2010;10:3223–30
   PubMed Web of Science ®Google Scholar