52
Views
80
CrossRef citations to date
0
Altmetric
Research Article

32P-Postlabeung Methods for DNA Adduct Detection: Overview and Critical Evaluation

&
Pages 67-85 | Published online: 22 Sep 2008

References

  • Doll R., Peto R. The Causes of Cancer. Oxford University Press, Oxford 1981
  • Miller E. C., Miller J. A. Searches for the ultimate chemical carcinogens and their reactions with cellular macromolecules. Cancer 1981; 47: 2327–2345
  • Hemminki K. Nucleic acid adducts of chemical carcinogens and mutagens. Arch. Toxicol. 1983; 52: 249–285
  • Bishop J. M. The molecular genetics of cancer. Science 1987; 235: 305–311
  • Weinberg R. A. Tumor suppressor genes. Science 1991; 254: 1138–1146
  • Ames B. N. Identifying environmental chemicals causing mutations and cancer. Science 1979; 204: 587–593
  • DeSerres F. J., Ashby J. Evaluation of Short-Term Tests for Carcinogens: Report of the International Collaborative Program. Elsevier, New York 1981
  • Stich H. F., San R. H. C. Short-Term Tests for Chemical Carcinogens. Springer-Verlag, New York 1981
  • Shamsuddin A. K. M., Sinopoli N. T., Hemminki K., Boesch R. R., Harris C. C. Detection of benzo(a)pyrene:DNA adducts in human white blood cells. Cancer Res. 1985; 45: 66–68
  • Harris C. C., Vahakangas K., Newman M. J., Trivers G. E., Shamsuddin A. K. M., Sinopoli N. T., Mann D. L., Wright W. E. Detection fo benzo(a)pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in sera from coke oven workers. Proc. Natl. Acad. Sci. USA 1985; 82: 6672–6676
  • Everson R. B., Randerath E., Santella R. M., Cefalo R. C., Avitts T. A., Randerath K. Detection of smoking related covalent DNA adducts in human placenta. Science 1986; 231: 54–57
  • Perera F. P., Hemminki K., Young T. L., Brenner D., Kelly G., Santella R. M. Detection of polycyclic aromatic hydrocarbon DNA adducts in white blood cells of foundry workers. Cancer Res. 1988; 48: 2288–2291
  • Vahakangas K., Haugen A., Harris C. C. An applied synchronous spectrophotometric assay to study benzo(a)pyrene diol epoxide-DNA adducts. Carcinogenesis 1985; 6: 1109–1116
  • Weston A., Rowe M. L., Manchester D. K., Farmer P. B., Mann D. L., Harris C. C. Fluorescence and mass spectral evidence for the formation of benzo(a)pyrene antidiol epoxide-DNA and hemoglobin adducts in humans. Carcinogenesis 1989; 10: 251–257
  • Randerath E., Avitts T. A., Reddy M. V., Miller R. H., Everson R. B., Randerath K. Comparative 32P-analysis of cigarette smoke induced DNA damage in human tissues and mouse skin. Cancer Res. 1986; 46: 5869–5877
  • Randerath E., Randerath K. Monitoring tobacco smoke-induced DNA damage by 32P-postlabeling. Postlabelling Methods for the Detection of DNA Adducts. IARC. Sci. Publ. 124, International Agency for Research on Cancer, LyonFrance 1993; 305–314
  • Reddy M. V., Hemminki K., Randerath K. Postlabeling analysis of polycyclic aromatic hydrocarbon-DNA adducts in white blood cells of foundry workers. J. Toxicol. Environ. Health 1991; 34: 177–185
  • Hemminki K., Grzybowska E., Chorazy M., Twardowska-Saucha K., Sroczynski J. W., Putman K. L., Randerath K., Phillips D. H., Hewer A. Aromatic DNA adducts in white blood cells of coke workers. Int. Arch. Occup. Environ. Health 1990; 62: 467–470
  • Darcey D. J., Everson R. B., Putman K. L., Randerath K. DNA adducts and exposure to burning oil. The Lancet 1992; 339: 489
  • Randerath K., Reddy M. V., Gupta R. C. 32P-Labeling test for DNA damage. Proc. Natl. Acad. Sci. USA 1981; 78: 6126–6129
  • Gupta R. C., Reddy M. V., Randerath K. 32P-Postlabeling analysis of nonradioactive aromatic carcinogen-DNA adducts. Carcinogenesis 1982; 3: 1081–1092
  • Reddy M. V., Gupta R. C., Randerath E., Randerath K. 32P-Postlabeling test for covalent DNA binding of chemicals in vivo: Application to a variety of aromatic carcinogens and methylating agents. Carcinogenesis 1984; 5: 231–243
  • Reddy M. V., Randerath K. Nuclease PI-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis 1986; 7: 1543–1551
  • Randerath K., Randerath E. 32P-Postlabeling analysis of mutagen- and carcinogen-DNA adducts and age-related DNA modifications (I-compounds). Molecular Dosimetry and Human Cancer: Analytical, Epidemiological, and Social Considerations, J. D. Groop-man, P. L. Skipper. CRC Press, Boca Raton, FL 1991; 131–150
  • Randerath K., Reddy M. V., Disher R. M. Age- and tissue-related DNA modifications in untreated rats: Detection by 32P-postlabeling assay and possible significance for spontaneous tumor induction and aging. Carcinogenesis 1986; 7: 1615–1617
  • Li D., Randerath K. Association between diet and age-related DNA modifications (I-compounds) in rat liver and kidney. Cancer Res. 1990; 50: 3991–3996
  • Randerath E., Randerath K., Reddy R., Lucier G. W. Sexual dimorphism of the chromatographic profiles of I-compounds (endogenous deoxyribonucleic acid modifications) in rat liver. Endocrinology 1991; 129: 3093–3100
  • Randerath K., Putman K. L., Randerath E., Mason G., Keliey M., Safe S. Organ-specific effects of long-term feeding of 2,3,7,8-tetrachlorodibenzo-dioxin and 1,2,3,7,8-pentachlorodibenzo-p-dioxin on I-compounds in hepatic and renal DNA of female Sprague-Dawley rats. Carcinogenesis 1988; 9: 2285–2289
  • Li D., Chandar N., Lombardi B., Randerath K. Reduced accumulation of I-compounds in liver DNA of rats fed a choline-devoid diet. Carcinogenesis 1989; 10: 605–607
  • Nath R. G., Li D., Randerath K. Acute and long-term effects of carbon tetrachloride on DNA modifications (I-compounds) in male mouse liver. Chem.-Biol. Interactions 1990; 76: 343–357
  • Randerath E., Randerath K., Reddy R., Danna T. F., Rao M. S., Reddy J. K. Induction of rat liver DNA alterations by chronic administration of peroxisome proliferators as detected by 32P-postlabeling. Mutat. Res. 1991; 247: 65–76
  • Li D., Moorthy B., Chen S., Randerath K. Effects of cytochrome P450 inducers on I-compounds in rat liver and kidney DNA. Carcinogenesis 1992; 13: 1191–1198
  • Randerath E., Agrawal H. P., Weaver J. A., Bordelon C. B., Randerath K. 32P-Postlabeling analysis of DNA adducts persisting for up to 42 weeks in the skin, epidermis and dermis of mice treated topically with 7,12-dimethylbenz(a)anthracene. Carcinogenesis 1985; 6: 1117–1126
  • Gupta R. C. Enhanced sensitivity of 32P-postlabeling analysis of aromatic carcinogen-DNA adducts. Cancer Res. 1985; 45: 5656–5662
  • Randerath K. E., Randerath T. F., Danna van Golen K. L., Putman K. L. A new sensitive 32P-postlabeling assay based on the specific enzymatic conversion of bulky DNA lesions to radiolabeled dinucle-otides and nucleoside 5′-monophosphates. Carcinogenesis 1989; 10: 1231–1239
  • Dunn B. P., San R. H. C. HPLC enrichment of hydrophobic DNA-carcinogen adducts for enhanced sensitivity of 32P-postlabeling analysis. Carcinogenesis 1988; 9: 1055–1060
  • Wilson V. L., Basu A. K., Essigman J. M., Smith R. A., Harris C. C. O6-Alkyldeoxyguanosine detection by 32P-postlabeling and nucleotide chromatographic analysis. Cancer Res. 1988; 48: 2156–2161
  • Watson W. P., Crane A. E. HPLC-32P-postlabeling analysis of 1, N6-ethenodeoxyadenosine and 3,yV4-ethenodeoxycytidine. Mutagenesis 1989; 75–77
  • Steiner S., Crane A. E., Watson W. P. Molecular dosimetry of DNA adducts in C3H mice treated with glycidaldehyde. Carcinogenesis 1992; 13: 119–124
  • Beach A. C., Gupta R. C. Human biomonitoring and the 32P-postlabeling assay. Carcinogenesis 1992; 13: 1053–1074
  • Gupta R. C., Randerath K. Analysis of DNA adducts by 32P-postlabeling and thin-layer chromatography. DNA Repair, a Laboratory Manual of Research Procedures, E. C. Friedberg, P. C. Hanawalt. Dekker, New York 1988; Vol. 3: 399–418
  • Randerath K., Lu L. -J.W., Li D. A comparison between different types of covalent DNA modifications (I-compounds, persistent carcinogen adducts and 5-methylcytosine) in regenerating rat liver. Carcinogenesis 1988; 9: 1843–1848
  • Schurdak M. E., Randerath K. Tissue-specific DNA adduct formation in mice treated with the environmental carcinogen 1H-dibenzo(c, g)carbazole. Carcinogenesis 1985; 6: 1271–1274
  • Randerath K., Haglund R. E., Phillips D. H., Reddy M. V. 32P-Postlabeling analysis of DNA adducts formed in the livers of animals treated with safrole, estragole and other naturally-occurring alkenyl-benzenes. I. Adult female CD-I mice. Carcinogenesis 1984; 5: 1613–1622
  • Phillips D. H., Reddy M. V., Randerath K. 32P-Postlabeling analysis of DNA adducts formed in the livers of animals treated with safrole, estragole and other naturally-occurring alkenylbenzenes. II. Newborn male B6C3F1 mice. Carcinogenesis 1984; 5: 1623–1628
  • Randerath E., Agrawal H. P., Reddy M. V., Randerath K. Highly persistent polycyclic aromatic hydrocarbon-DNA adducts in mouse skin: Detection by 32P-postlabeling analysis. Cancer Lett. 1983; 20: 109–114
  • Wogan G. N., Gorelick N. J. Chemical and biochemical dosimetry of exposure to genotoxic chemicals. Environ. Health Perspect. 1985; 62: 5–18
  • Randerath K., Agrawal H. P., Randerath E. 12-O-Tetradecanoyl-phorbol-13-acetate-induced rapid loss of persistent 7,12-dimethylbenzol-(a)anthracene-DNA adducts in mouse epidermis and dermis. Cancer Lett. 1985; 27: 35–43
  • Ames B. N., Gold L. S. Endogenous mutagens and the causes of cancer and aging. Mutat. Res. 1991; 250: 3–16
  • Halliwell B., Aruoma O. I. DNA damage by oxygen-derived species. FEBS Lett. 1991; 281: 9–19
  • Cadet J., Odin F., Mouret J. -F., Polverelli M., Audic A., Gia-comoni P., Favier A., Richard M. -J. Chemical and biochemical postlabeling methods for singling out specific oxidative DNA lesions. Mutat. Res. 1992; 275: 343–354
  • Lu L. -J., Tasaka F., Hokanson J. A., Kohda K. Detection of 8-hydroxy-2′-deoxyguanosine in deoxyribonucleic acid by the 32P-postlabeling method. Chem. Pharm. Bull. 1991; 39: 1880–1882
  • Randerath K., Reddy R., Danna T. F., Watson W. P., Crane A. E., Randerath E. Formation of ribonucleotides in DNA modified by oxidative damage in vitro and in vivo. Characterization by 32P-postlabel-ing. Mutat. Res. 1992; 275: 355–366
  • Lutgerink J. T., deGraaf E., Hoebee B., Stavenuiter F. C., Westra J. G., Kriek E. Detection of 8-hydroxydeoxyguanine in small amounts of DNA by 32P-postlabeling. Anal. Biochem. 1992; 201: 127–133
  • Hegi M. E., Sagelsdorff P., Lutz W. L. Detection by 32P-postlabeling of thymidine glycol in gamma-irradiated DNA. Carcinogenesis 1989; 10: 43–47
  • Reddy M. V., Bleicher W. T., Blackburn G. R. 32P-Postlabeling detection of thymine glycols: Evaluation of adduct recoveries after enhancement with affinity chromatography, nuclease PI, nuclease SI, and polynucleotide kinase. Cancer Commun. 1991; 3: 109–117
  • Randerath K., Yang P. -F., Danna T. F., Reddy R., Watson W. P., Randerath E. Bulky adducts detected by 32P-postlabeling in DNA modified by oxidative damage in vitro. Comparison with rat lung I-compounds. Mutat. Res 1991; 250: 135–144
  • Carmichael P. L., Shé M. N., Phillips D. H. Detection and characterization by 32P-postlabeling of DNA adducts induced by a Fenton-type oxygen radical-generating system. Carcinogenesis 1992; 13: 1127–1135
  • Weinfeld M., Soderlind K. -J. 32P-Postlabeling detection of radiation-induced DNA damage: Identification and estimation of thymine glycols and phosphoglycolate termini. Biochemistry 1991; 30: 1091–1097
  • Everson R. B., Randerath E., Santella R. M., Avitts T. A., Weinstein I. B., Randerath K. Quantitative associations between DNA damage in human placenta and maternal smoking and birth weight. J. Natl. Cancer Inst. 1988; 80: 567–576
  • Randerath E., Mittal D., Randerath K. Tissue distribution of co-valent DNA damage in mice treated dermally with cigarette “tar”: Preference for lung and heart DNA. Carcinogenesis 1988; 9: 75–80
  • Phillips D. H., Hewer A., Martin C. N., Garner R. C., King M. M. Correlation of DNA adduct levels in human lung with cigarette smoking. Nature 1988; 336: 790–792
  • Randerath E., Miller R. H., Mittal D., Avitts T. A., Dunsford H. A., Randerath K. Covalent DNA damage in tissues of cigarette smokers as determined by 32P-postlabeling assay. J. Natl. Cancer Inst. 1989; 81: 341–347
  • United States Department of Health and Human Services. The Health Consequences of Smoking: Cancer. A Report of the Surgeon General. Office on Smoking and Health, Rockville, MD 1982
  • IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Tobacco Smoking. International Agency for Research on Cancer, LyonFrance 1985; Vol. 38
  • Bond J. A., Chen B. T., Griffith W. C., Mauderly J. L. Inhaled cigarette smoke induces the formation of DNA adducts in lungs of rats. Toxicol. Appl. Pharmacol. 1989; 99: 161–172
  • Gairola C. G., Gupta R. C. Cigarette smoke-induced DNA adducts in the respiratory and nonrespiratory tissues of rats. Environ. Mol. Mutagen. 1991; 17: 253–257
  • Randerath E. T, Danna F., Randerath K. DNA damage induced by cigarette smoke condensate in vitro as assayed by 32P-postlabeling. Comparison with cigarette smoke-associated DNA adduct profiles in vivo. Mutat. Res. 1992; 268: 139–153
  • Savela K., Hemminki K., Hewer A., Phillips D. H., Putman K. L., Randerath K. Interlaboratory comparison of the 32P-postlabeling assay for aromatic DNA adducts in white blood cells of iron foundry workers. Mutat. Res. 1989; 224: 485–492
  • Hemminki K., Grzybowska E., Chorazy M., Twardowska-Saucha K., Sroczynski J. W., Putman K. L., Randerath K., Phillips D. H., Hewer A., Santella R. M., Young T L., Perera F. P. DNA adducts in humans environmentally exposed to aromatic compounds in an industrial area of Poland. Carcinogenesis 1990; 11: 1229–1231
  • Randerath K., Li D., Randerath E. Age-related DNA modifications (1-compounds): Modulation by physiological and pathological processes. Mutat. Res. 1990; 238: 245–253
  • Li D., Chen S., Randerath K. Natural dietary ingredients (oats and alfalfa) induce covalent DNA modifications (I-compounds) in rat liver and kidney. Nutr. Cancer 1992; 17: 205–216
  • Li D., Randerath K. Modulation of DNA modification (I-compound) levels in rat liver and kidney by dietary carbohydrate, protein, fat, vitamin, and mineral content. Mutat. Res. 1992; 275: 47–56
  • Moorthy van Golen B. K.L., Randerath K. Sex-specific modulation of hepatic covalent DNA modifications (I-compounds) by the cytochrome P450 inducer, pregnenolone-16a-carbonitrile. Toxicol. Appl. Pharmacol. 1992; 113: 218–226
  • Li D., Xu D., Randerath K. Species and tissue specificities of I-compounds as contrasted with carcinogen adducts in liver, kidney and skin DNA of Sprague-Dawley rats, ICR mice and Syrian hamsters. Carcinogenesis 1990; 11: 2227–2232
  • Randerath K., Liehr J. G., Gladek A., Randerath E. Age-dependent covalent DNA alterations (I-compounds) in rodent tissues: Species, tissue and sex specificities. Mutat. Res. 1989; 219: 121–133
  • Randerath K., Putman K. L., Osterburg H. H., Johnson S. A., Morgan D. G., Finch C. E. Age-dependent increases of DNA adducts (I-compounds) in human and rat brain DNA. Mutat. Res. 1993; 295: 11–18
  • Randerath E., Hart R. W., Turturro A., Danna T. F., Reddy R., Randerath K. Effects of aging and caloric restriction on I-compounds in liver, kidney, and white blood cell DNA of male Brown-Norway rats. Mech. Ag. Devel. 1991; 58: 279–296
  • Randerath K., Hart R. W., Zhou G. -D., Reddy R., Danna T. F., Randerath E. Enhancement of age-related increases in DNA I-compound levels by calorie restriction: Comparison of male B-N and F-344 rats. Mutat. Res. 1993; 295: 31–46
  • Nath R. G., Randerath E., Randerath K. Short-term effects of the tumor promoting polychlorinated biphenyl mixture, Aroclor 1254, on 1-compounds in liver, kidney, and lung DNA of male Sprague-Dawley rats. Toxicology 1991; 68: 275–289
  • Li D., Xu D., Chandar N., Lombardi B., Randerath K. Persistent reduction of I-compound levels in liver DNA from male Fischer rats fed choline-devoid diet and in DNA of resulting neoplasms. Cancer Res. 1990; 50: 7577–7580
  • Randerath K., Randerath E., Danna T. F. Lack of I-compounds in DNA from a spectrum of Morris hepatomas. Carcinogenesis 1990; 11: 1041–1044
  • Li D., Chen S., Becker F. F., Randerath K. Specific reduction of I-compound levels in DNA from spontaneous hepatomas of 22–24 month old male C3H mice. Carcinogenesis 1991; 12: 2389–2391
  • LiRanderath D. K. Strain differences of I-compounds in relation to organ sites of spontaneous tumorigenesis and non-neoplastic renal disease in mice. Carcinogenesis 1990; 11: 251–255
  • Liehr J. G., Avitts T. A., Randerath E., Randerath K. Estrogen-induced endogenous DNA adduction: Possible mechanism of hormonal cancer. Proc. Natl. Acad. Sci. USA 1986; 83: 5301–5305
  • Nath R. G., Vulimiri S. V., Randerath K. Circadian rhythm of covalent modifications in liver DNA. Biochem. Biophys. Res. Com-mun. 1992; 189: 545–550
  • Randerath K., Zhou G. -D., Hart R. W., Turturro A., Randerath E. Biomarkers of aging: Correlation of DNA I-compound levels with median lifespan of calorically restricted and ad libitum fed rats and mice. Mutat. Res. 1994, in press

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.