Publication Cover
Hemoglobin
international journal for hemoglobin research
Volume 39, 2015 - Issue 3
210
Views
7
CrossRef citations to date
0
Altmetric
Original Article

Identification of Hb Constant Spring (HBA2: c.427T > C) by an Automated High Performance Liquid Chromatography Method

, &
Pages 190-195 | Received 03 Feb 2015, Accepted 25 Feb 2015, Published online: 27 May 2015

References

  • Gibbons R, Higgs DR, Olivieri NF, Wood WG. The thalassemia intermedia. In: Weatherall DJ, Clegg JB, Eds. The Thalassaemia Syndromes, 3rd ed. London, UK: Blackwell Scientific Publication. 1981:563–571
  • Clegg JB, Weatherall DJ, Milner PF. Hemoglobin Constant Spring, a chain determination. Nature. 1971;234(5328):334–337
  • Pootakul P, Winichagoon P, Fucharoen S, et al. Homozygous Hemoglobin Constant Spring: A need for revision concept. Hum Genet. 1981;59(3):250–255
  • Wilairat P. Local problem, global implication. Science Asia. 2007;33(Suppl 1):81–83
  • Bartlett RC. Rapid cellulose acetate electrophoresis. II. Qualitative and quantitative hemoglobin fractionation. Clin Chem. 1963;9:325–329
  • Schmidt RM, Holland S. Standardization in abnormal hemoglobin detection. An evaluation of hemoglobin electrophoresis kits. Clin Chem. 1974;20(5):591–594
  • Tan GB, Aw TC, Dunstan RA, Lee SH. Evaluation of high performance liquid chromatography for routine estimation of Haemoglobins A2 and F. J Clin Pathol. 1993;46(9):852–856
  • Fucharoen S, Winichagoon P, Wisedpanichkij R, et al. Prenatal and postnatal diagnosis of thalassemias and hemoglobinopathies by HPLC. Clin Chem. 1998;44(4):740–748
  • Clarke GM, Higgins TN. Laboratory investigation of hemoglobinopathies and thalassemias: Review and update. Clin Chem. 2000;46(8B):1284–1290
  • Winichagoon P, Sriphanich R, Sae-Ngow B, et al. Application of automated HPLC in prenatal diagnosis of thalassemia. Lab Hematol. 2002;8(1):29–35
  • Technicon H*3 RTX™ system user’s manual. Bayer, New York, NY, USA
  • VARIANT™ β-Thalassemia Short Program instruction manual. Bio-Rad Laboratory Diagnostics Groups, Hercules, CA, USA
  • Baysal E, Huisman THJ. Detection of common deletional α-thalassemia-2 determinants by PCR. Am J Hematol. 1994;46(3):208–213
  • Chen TP, Lin SF, Chang JG, et al. Molecular characterization of Hb H disease by polymerase chain reaction. Acta Haematol. 1993;90(4):177–181
  • Fucharoen S, Fucharoen G, Fukumaki Y. Simple non radioactive method for detecting Haemoglobin Constant Spring gene. Lancet. 1990;335(8704):1527
  • Thongglairoam V, Winichagoon P, Fucharoen S, et al. Hemoglobin Constant Spring in Bangkok: Molecular screening by selective enzymatic amplification of the α2-globin gene. Am J Hematol. 1991;38(4):277–280
  • Singsanan S, Fucharoen G, Savongsy O, et al. Molecular characterization and origins of Hb Constant Spring and Hb Paksé in Southeast Asian populations. Ann Hematol. 2007;86(9):665–669
  • Viprakasit V, Tanphaichitr VS, Pung-Amritt P, et al. Clinical phenotypes and molecular characterization of Hb H-Paksé disease. Haematologica. 2002;87(2):117–125
  • Sanchaisuriya K, Fucharoen G, Fucharoen S. Hb Paksé [(α2) codon 142 (TAA→TAT or Term→Tyr)] in Thai patients with EABart’s disease and Hb H disease. Hemoglobin. 2002;26(3):227–235
  • Fucharoen S, Sanchaisuriya K, Fucharoen G, et al. Interaction of Hemoglobin E and several forms of α-thalassemia in Cambodian families. Haematologica. 2003;88(10):1092–1098
  • Liao C, Zhou JY, Xie XM, et al. Detection of Hb Constant Spring by a capillary electrophoresis method. Hemoglobin. 2010;34(2):175–178
  • Waneesorn J, Panyasai S, Kongthai K, et al. Comparison between capillary electrophoresis and high performance liquid chromatography for detection and quantification of Hb Constant Spring [Hb CS; α142, Term→Gln (TAA > CAA in α2]. Hemoglobin. 2011;35(4):338–345
  • Hunt DM, Higgs DR, Winichagoon P, et al. Haemoglobin Constant Spring has an unstable α chain messenger RNA. Br J Haematol. 1982;51(3):405–413
  • Weiss I, Liebhaber SA. Destabilization of α-globin Constant Spring mRNA is mediated by translation into the 3′ non coding region. Blood. 1990;76(Suppl):abstract 80a

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.