36
Views
0
CrossRef citations to date
0
Altmetric
Original Article

New Developments in Protein Isolation, Purification, and Characterization

Technology

Pages 71-84 | Published online: 11 Jun 2009

References

  • Scopes R K. Protein Purification, Principles and Practice 2nd ed. Springer-Velag, New York, Berlin, Heidelberg 1987; 21–71
  • MacManus J P, Hutnik C NL, Sykes B D. Characterization and site-specific mutagenesis of the calcium-binding protein on-comodulin produced by recombinant bacteria. J Biol Chem 1989; 264: 3470–3477
  • Torres A R, Krueger G G, Peterson E A. Purification of Gc-2 globulin from human serum by displacement chromatography: A model for the isolation of marker proteins identified by two-dimensional electrophoresis. Anal Biochem 1985; 144: 469–476
  • Dunn B E, Edberg S E, Torres A R. Purification of Escherichia coli alkaline phosphatase on an ion-exchange high-performance liquid chromatography column using carboxymethyl dextrans. Anal Biochem 1988; 168: 25–30
  • Torres A R, Peterson E A. Purification of monoclonal antibodies by complex-displacement chromatography on CM-cellulose. J. Chromatogr 1990; 499: 47–57
  • Sluyterman L AA, Elgersma O. Chromatofbcusing: isoelectric focusing on ion-exchange columns. I. General principles. J Chromatogr 1978; 150: 17–30
  • Sluyterman L AA, Elgersma O. Chromatofocusing: isoelectric focusing on ion-exchange columns. U. Experimental verification. J Chromatogr 1978; 150: 31–44
  • Coufalic A H, Feldmon M, Metal Platica. Use of chromatofocusing to distinguish estradiol receptor from ovarian-dependent and -independent rat mammary tumors. Cancer Res 1983; 43: 5325–5239
  • Hutchens T W, Gibbons W E, Besch P K. High-performance chromatofocusing and size-exclusion chromatography: separation of human uterine estrogen-binding proteins. J Chromatogr 1984; 297: 283–299
  • Porath J, Carlsson J, Olsson I. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 1975; 258: 598–599
  • Sulkowski E. Purification of proteins by IMAC. Trends Biotechnol 1985; 3: 1–7
  • Hemdan E S, Zhao Y-U, Sulkowski E. Surface topography of histidine residues: A facile probe by immobilized metal ion affinity chromatography. Proc Natl Acad Sci (USA) 1989; 86: 1811–1815
  • Sulkowski E. Immobilized metal ion affinity chromatography of proteins. Protein Purification: Micro or Macro, R Burger. Alan R Liss, New York 1987; 149–162
  • Andersson L, Sulkowski E, Porath J. Facile resolution of a-fetoproteins and serum albumin by immobilized metal ion affinity chromatography. Cancer Res 1987; 47: 3624–3626
  • Weselake R J, Chesney S L, Petkau A: Purification of human copper, zinc. Superoxide dismutase by copper chelate affinity chromatography. Anal Biochem 1986; 155: 193–197
  • Utsumi J, Yamamoto-Teraswa I. Elimination of contaminating Escherichi coli peptides in the purification of Escherichia coli-derived recombinant human interferon-β1, by zinc chelate chromatography. J Chromatogr 1989; 490: 193–197
  • Hammacher A, Hellman U, Johnsson A. A major part of PDGF purified from human platelets is a heterodimer of one A and one B chain. J Biol Chem 1988; 263: 16493–16498
  • Anderson L, Porath J. Isolation of phosphoproteins by immobilized metal (Fe3+) ion affinity chromatography. Anal Biochem 1986; 154: 250–254
  • Muszynska G, Andersson L, Porath J. Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. Biochemistry 1986; 25: 6850–6853
  • Chaga G, Andersson L, Ersson B. Purification of two muscle enzymes by chromatography on immobilized ferric ions. Biotech Appl Biochem 1989; 11: 424–431
  • Fatiadi J A. Affinity chromatography and metal chelate affinity chromatography. CRC Crit Rev Anal Chem 1987; 18: 1–44
  • Brocklehurst K, Carlsson J, Kierstan P J. Covalent chromatography. Preparation of fully active papain from dried papaya latex. Biochem J 1973; 133: 573–589
  • Rydén L, Eaker D. Identification of the thiol groups in human ceruloplasmin. Eur I Biochem 1983; 132: 241–247
  • Porath J, Maisano F, Belew M. Thiophilic adsorption–a new method for protein fractionation. FEBS Letters 1985; 185: 300–310
  • Porath I. Metal ion, hydrophobic, thiophilic and π-electron governed interactions and their application to salt-promoted protein adsorption chromatography. Biotech Prog 1987; 3: 14–21
  • Demiroglou A, Jennissen H P. Synthesis and properties of butylagaroses prepared by the tresylchloride method. Biol Chem Hoppe-Seyler 1987; 368: 739
  • Oscarsson S, Porath J. Protein chromatography with pyridine- and alkylthioether-based agarose adsorbents. J Chromatogr 1990; 499: 235–247
  • Carlson J, Jansson J-C, Sparrman M. Affinity chromatography. Protein Purification, J-C Jansson, L. Rydén. VCH Publishers, New York, Weinheim, FRG, CambridgeUK 1989; 275–329
  • Trayer I P. Affinity chromatography: The current status. Int J Biochem 1977; 8: 177–181
  • Trayer I P. Affinity chromatography. Techniques in the Life Sciences. Elsevier/North Holland, Amsterdam 1984; Vol. BS103: 1–55, Suppl
  • Hirai H. A collaborative clinical study of carcinoembryonic antigen in Japan. Cancer Res 1987; 37: 2267–2279
  • Secher D S, Burke O C. A monoclonal antibody for large scale purification of human leucocyte interferon. Nature 1980; 285: 446–458
  • Antonini E, Rossi-Fane M MR. Immobilizedhemoproteins. Meth Enzymol 1976; 44: 538–546
  • Chiancone E, Gottoni M. Use of immobilized subunits for the purification of oligomeric and self-associating proteins. Meth Enzymol 1987; 135: 484–491
  • Chiancone E, Winzor D J. Subunit exchange chromatography of self-associating proteins: a quantitative appraizal. Anal Biochem 1986; 158: 211–216
  • Wilchek M, Bayer E. A universal affinity column using avidin-biotin technology. Protein Recognition of Immobilized Ugands. 1987; Vol. 80: 83–92, UCLA Symposium on Molecular and Cellular Biology
  • Buckie J W, Cook G MW. Specific isolation of surface glycoproteins from intact cells by biotinylated concanavalin A and immobilized streptavidin. Anal Biochem 1984; 156: 463–472
  • Hnatowich D J, Virzi F, Ruschkovski M. Investigation of avidin and biotin for imaging applications. J Nucl Med 1987; 86: 1294–1302
  • Sessenfeld H M, Brewer S J. A polypeptide fusion designed for the purification of recombinant proteins. Bio/Technol 1984; 2: 76–81
  • Smith M C, Furman T C, Ingolia T D. Chelating peptide-immobilized metal ion affinity chromatography. J Biol Chem 1988; 263: 7211–7215
  • Hochuli E. Large scale purification of recombinant proteins. J Chromatogr 1988; 44: 293–302
  • Mosbach K. Novel approaches to affinity chromatography. Macromol Chem Macromol Symp 1988; 17: 325–333
  • Scopes R K. Quantitative studies of ion-exchange and affinity elu-tion chromatography of enzymes. Anal Biochem 1981; 114: 8–18
  • Davies J R, Scopes R K: Purification of some tricarboxylic cycle enzymes from beef heart using affinity elution chromatography. Anal Biochem 1981; 114: 19–27
  • Yon R J: Versatility of mixed-function adsorbents in biospecific protein desorption: accidental affinity and an improved purification of aspartic transcarbamoylase from wheat germ. Anal Biochem 1981; 113: 219–228
  • Albertsson P-Å, Birkenmeier G. Affinity separation of proteins in aqueous three-phase systems. Anal Biochem 1988; 174: 154–161
  • Tjerneld F, Johansson G, Joelsson M. Affinity, liquid-liquid extraction of lactate dehydrogenase in large scale. Biotechnol Bioeng 1987; 30: 809–816
  • Wuenschell G E, Naranjo E, Arnold F H. Aqueous two-phase metal affinity extraction of heme proteins. Bioproc Eng 1990; 5: 199–202
  • Crane L J, Henry M D. Design requirements for silica-based chromatographic matrixes. Protein Recognition of Immobilized Ugands. 1987; Vol. 80: 83–92, UCLA Symp Mol Cell Biol
  • Ugelstad J, Soderberg L, Berge A. Monodisperse polymer particles–a step forward for chromatography. Nature 1983; 303: 95–96
  • Cachia C, Flamion C-J, Schreiber J-P. Purification of core salt-extracted E. coU 30S ribosomal proteins by high-performance liquid chromatography under non-denaturating conditions. J Chromatogr 1990; 498: 417–422
  • Stillian J R, Pohl C. New latex-bonded pellicular ion-exchangers with multiphase-selectivity for high-performance chromatographic separations. J Chromatogr 1990; 499: 249–266
  • Unger K K, Jilge J, Kinker JN. Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. II. Performance of non-porous monodisperse 1.5 ¨m silica beads in the separation of proteins by reversed phase gradient elution high-performance chromatography. J Chromatogr 1986; 359: 61–72
  • Khalgatki K. Micropellicular stationary phases for rapid protein analysis by high-performance liquid chromatography. J Chromatogr 1990; 499: 267–278
  • Rubinstein M. Preparative high-performance liquid partition chromatography of proteins. Anal Biochem 1979; 98: 1–7
  • Jeppsson J O, Kallman I, Lindgren G. Hb-Linköping (β36 Pro→Thr): a new hemoglobin mutant characterized by reverse-phase high-performance liquid chromatography. J Chromatogr 1984; 297: 31–36
  • Lu X M, Figueroa A, Karger B L. Intrinsic fluorescence and HPLC measurement of the surface dynamics of lysozyme adsorbed on hydrophobic silica. J Am Chem Soc 1978–1979; 110, 1988
  • Porath J, Sundberg L, Fornstedt N. Salting out in amphiphilic gels as a new approach to hydrophobic adsorption. Nature 1973; 245: 465–466
  • Hjertien S, Yao K, Eriksson K-E. Gradient and isocratic high-performance hydrophobic interaction chromatography of proteins on agarose columns. J Chromatogr 1986; 359: 99–109
  • Fausnaugh J L, Pfannkoch E, Gupra S. High-performance hydrophobic interaction chromatography of proteins. Anal Biochem 1984; 137: 464–472
  • Melander W, Horvath C. Salt effects on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series. Arch Biochem Biophys 1977; 183: 200–215
  • Kato Y, Kitamura T, Hashimoto T. Preparative high-performance hydrophobic interaction chromatography of proteins on TSK gel phenyl-5PW. J Chromatogr 1985; 333: 202–210
  • Oakley B R, Kirsch D R, Morris N R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 1980; 105: 361–363
  • Bjellqvist B, Ek K, Righetti P G. Immobilized pH gradients for isoelectric focusing. I. Theory and methodology. Protides Biol Fluid 1982; 30: 599–602
  • Righetti P G, Ek K, Bjellqvist B. Polymerization kinetics of polyacrylamide gels containing immobilized gradients for isoelectric focusing. J Chromatogr 1984; 291: 31–42
  • Haff L A, Fägerstam L, Barry A R. Use of electrophoretic titration curves for predicting optimal chromatographic conditions for fast ion-exchange chromatography of proteins. J Chromatogr 1983; 266: 409–425
  • Horejsi V. Some theoretical aspects of affinity electrophoresis. J Chromatogr 1979; 178: 1–13
  • Horejsi V, Ticha M, Tichy P. Affinity electrophoresis: New simple and general methods of preparation of affinity gels. Anal Biochem 1982; 125: 358–369
  • Fries E, Hjerten S. Scanning of polyacrylamide gel electrophoresis columns for detection of unstained protein zones and for their localization relative to enzyme activities. Anal Biochem 1975; 64: 466–476
  • Laskey R A. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Meth Enzymol 1980; 65: 363–371
  • Gershoni J M, Palade G E. Protein blotting: Principles and applications. Anal Biochem 1983; 131: 1–15
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc Natl Acad Sci (USA) 1979; 76: 4350–4354
  • Yuen K CL, Johnson T K, Denell T E. A silver-staining technique for detecting minute quantities of protein on nitrocellulose paper: retention of antigenicity of stained proteins. Anal Biochem 1982; 126: 398–402
  • Wojtkowiak Z, Briggs R C, Hnilica L S. A sensitive method for staining proteins transferred to nitrocellulose sheets. Anal Biochem 1983; 129: 486–489
  • Reiser J, Wardale J. Immunological detection of specific proteins in total cell extracts by fractionation in gels and transfer to diazophenylthioether paper. Eur J Biochem 1981; 114: 569–575
  • Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987; 262: 10035–10038
  • Cardin F S, Witt K R, Jackson R L. Visualization of heparin-binding proteins by ligand blotting with l25I-heparin. Anal Biochem 1984; 137: 368–373
  • Gorelick F S, Conn J A, Freedman S D. Calmodulin-stimulated protein-kinase activity from rat pancreas. J Cell Biol 1983; 97: 1294–1298
  • Olmsted J B. Affinity purification of antibodies from diazotized paper blots of heterogeneous proteins samples. J Biol Chem 1955; 256: 1, 11957, 1981
  • Anderson N L, Taylor J, Scandora A E. The TYCHO system for computer analysis of two-dimensional gel electrophoresis patterns. Clin Chem 1981; 27: 1807–1820
  • Jackson P, Thompson R J. The immunodetection of brain proteins blotted onto nitrocellulose from fixed and stained two-dimensional polyacrylamide gels. Electrophoresis 1984; 5: 35–42
  • Geysen J, DeLoof A, Vandesande F. How to perform subsequent or double immunostaining of two different antigens on a single nitrocellulose blot within one day with an immunoperoxidase technique. Electrophoresis 1984; 5: 129–131
  • Jonsson G P, Hedin A B, Hakansson P L. Plasma desorption mass spectrometry of peptides and proteins adsorbed on nitrocellulose. Anal Chem 1986; 56: 1084–1087
  • Norrild B, Bjerrum O J, Vestergaard B F. Polypeptide analysis of individual immunoprecipitates from crossed immunoelectro-phoresis. Anal Biochem 1977; 81: 432–441
  • Lizana J, OlssonSavili I. T. Immunofixation-agarose isoelectric focusing as applied to the analysis of transferrin, alfa,-antitrypsin and Gc-globulin. Clin Chem 1982; 28: 1569
  • O'Farrell P H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975; 250: 4004–4021
  • Anderson N L, Anderson N G. High resolution two-dimensional electrophoresis of human plasma proteins. Proc Natl Acad Sci (USA) 1977; 74: 5421–5425
  • Anderson N G, Anderson N L. Analytical techniques for cell fractions. Anal Biochem 1978; 85: 331–354
  • Westermeier R, Poster W, Wester J. High resolution two-dimensional electrophoresis with isoelectric focusing in immobilized pH-gradients. J. Biochem Biophys Meth 1983; 8: 321–330
  • Neidhardt F C, Vaughn V, Phillips T A. Gene-protein index of Escherichia coli K 12. Microbiol Rev 1983; 47: 231–284
  • White M D, Ralston G B. Two-dimensional poly aery lamide gel electrophoresis of water-soluble membrane proteins. Electrophoresis 1981; 2: 240–246
  • Lester E P, Lemkin P F, Lowery J F. Human leukemias: A preliminary 2-D electrophoretic analysis. Electrophoresis 1982; 3: 364–375
  • Jones M I, Spragg S P. Analysis of fetal peptides in human amniotic fluid and in fetal fluids and tissues by two-dimensional electrophoresis. Electrophoresis 1983; 4: 291–297
  • Ozturk M, Motté P, Takahasi H. Identification and characterization of a Mr 50,000 adrenal protein in human hepatocellular carcinoma. Cancer Res 1989; 49: 6764–6773
  • Hjerten S: High-performance electrophoresis. Elimination of elec-troendosmosis and solute adsorption. J Chromatogr 1985; 347: 191–198
  • Liidi L, Gassmann E: Capillary zone electrophoresis for the analysis of peptides synthesized by recombinant DNA technology. Anal Chim Acta 1988; 213: 215–219
  • Olivares J A, Nguyen N T, Yonker C R. On-line mass spec-trometric detection for capillary zone electrophoresis. Anal Chem 1987; 59: 1230–1232
  • Terabe S, Otsuka K, Ichikawa K. Electrokinetic separations with micellar solutions and open-tubular capillariesa. Anal Chem 1984; 56: 111–113
  • Terabe S. Electrokinetic chromatography: an interface between electrophoresis and chromatography. Trends Anal Chem 1989; 8: 129–134
  • Compton S W, Brownlee R G. Capillary electrophoresis. Biotech-niques 1988; 6: 432–40
  • Kilar F, Hjerten S. Fast and high resolution analysis of human serum transferrin by high performance isoelectric focusing in capillaries. Electrophoresis 1989; 10: 23–29
  • Cohen A S, Karger B L. High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins. J Chromatogr 1987; 397: 409–417
  • Guttman A, Paulus A, Cohen A S. Use of complexing agents for selective separation in high-performance capillary electrophoresis. Chiral resolution via cyclodextrins incorporated with polyacrylamide gel columns. J Chromatogr 1988; 448: 41–53

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.