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Review Article

Harnessing biodiesel-producing microbes: from genetic engineering of lipase to metabolic engineering of fatty acid biosynthetic pathway

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Pages 26-36 | Received 16 Mar 2015, Accepted 09 Sep 2015, Published online: 02 Nov 2015

References

  • Adachi D, Hama S, Nakashima K, et al. (2013a). Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B. Bioresour Technol, 135, 410–16
  • Adachi D, Hama S, Numata T, et al. (2011). Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. Bioresour Technol, 102, 6723–9
  • Adachi D, Koh FH, Hama S, et al. (2013b). A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: characterization and application to enzymatic biodiesel production. Enzyme Microb Technol, 52, 331–5
  • Ageitos JM, Vallejo JA, Veiga-Crespo P, Villa TG. (2011). Oily yeasts as oleaginous cell factories. Appl Microbiol Biotechnol, 90, 1219–27
  • Beopoulos A, Cescut J, Haddouche R, et al. (2009). Yarrowia lipolytica as a model for bio-oil production. Prog Lipid Res, 48, 375–87
  • Bokinsky G, Peralta-Yahya PP, George A, et al. (2011). Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci USA, 108, 19949–54
  • Christopher LP, Kumar H, Zambare VP. (2014). Enzymatic biodiesel: challenges and opportunities. Appl Energy, 119, 497–520
  • Colin VL, Rodríguez A, Cristóbal HA. (2011). The role of synthetic biology in the design of microbial cell factories for biofuel production. J Biomed Biotechnol, 2011, 1–9
  • Duan YK, Zhu Z, Cai K, et al. (2011). De novo biosynthesis of biodiesel by Escherichia coli in optimized fed-batch cultivation. PLoS One, 6, e20265
  • Fan X, Niehus X, Sandoval G. (2012). Lipases as biocatalyst for biodiesel production. Methods Mol Biol, 861, 471–83
  • Fan L, Liu J, Nie K, et al. (2013). Synthesis of medium chain length fatty acid ethyl esters in engineered Escherichia coli using endogenously produced medium chain fatty acids. Enzyme Microb Technol, 53, 128–33
  • Fernandez-Lafuente R. (2010). Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst. J Mol Catal B Enzym, 62, 197–212
  • Fjerbaek L, Christensen KV, Norddahl B. (2009). A review of the current state of biodiesel production using enzymatic transesterification. Biotechnol Bioeng, 102, 1298–315
  • Fukuda H, Hama S, Tamalampudi S, Noda H. (2008). Whole-cell biocatalysts for biodiesel fuel production. Trends Biotechnol, 26, 668–73
  • Gao B, Su EZ, Lin JP, et al. (2009). Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production. J Biotechnol, 139, 169–75
  • Gog A, Roman M, Tosa M, et al. (2012). Biodiesel production using enzymatic transesterification – current state and perspectives. Renew Energy, 39, 10–16
  • Guan FF, Peng P, Wang GL, et al. (2010). Combination of two lipases more efficiently catalyzes methanolysis of soybean oil for biodiesel production in aqueous medium. Process Biochem, 45, 1677–82
  • Hama S, Numata T, Tamalampudi S, et al. (2009). Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters. J Mol Catal B Enzym, 58, 93–7
  • Hama S, Tamalampudi S, Suzuki Y, et al. (2008). Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. Appl Microbiol Biotechnol, 81, 637–45
  • Hernández-Martín E, Otero C. (2008). Different enzyme requirements for the synthesis of biodiesel: Novozym 435 and Lipozyme TLIM. Bioresour Technol, 99, 277–86
  • Howard TP, Middelhaufe S, Moore K, et al. (2010). Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts. J Mol Catal B Enzym, 66, 101–4
  • Huang DF, Han SY, Han ZL, Lin Y. (2012). Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system. Biochem Eng J, 63, 10–14
  • Hwang HT, Qi F, Yuan C, et al. (2014). Lipase-catalyzed process for biodiesel production: protein engineering and lipase production. Biotechnol Bioeng, 111, 639–53
  • Jeon E, Lee S, Lee S, et al. (2012). Improved production of long-chain fatty acid in Escherichia coli by an engineering elongation cycle during fatty acid synthesis (FAS) through genetic manipulation. J Microbiol Biotechnol, 22, 990–9
  • Jeon E, Lee S, Won JI, et al. (2011). Development of Escherichia coli MG1655 strains to produce long chain fatty acids by engineering fatty acid synthesis (FAS) metabolism. Enzyme Microb Technol, 49, 44–51
  • Jin Z, Han SY, Zhang L, et al. (2013). Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media. Bioresour Technol, 130, 102–9
  • Kalscheuer R, Luftmann H, Steinbüchel A. (2004). Synthesis of novel lipids in Saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase. Appl Environ Microbiol, 70, 7119–25
  • Kalscheuer R, Stölting T, Steinbüchel A. (2006). Microdiesel: Escherichia coli engineered for fuel production. Microbiology, 152, 2529–36
  • Kim S, Song JK, Kim HK. (2013). Cell surface display of Staphylococcus haemolyticus L62 lipase in Escherichia coli and its application as a whole cell biocatalyst for biodiesel production. J Mol Catal B Enzym, 97, 54–61
  • Lam MK, Lee KT, Mohamed AR. (2010). Homogeneous, heterogeneous and enzymatic catalysis for transesterification of high free fatty acid oil (waste cooking oil) to biodiesel: a review. Biotechnol Adv, 28, 500–18
  • Lee JW, Na D, Park JM, et al. (2012). Systems metabolic engineering of microorganisms for natural and non-natural chemicals. Nat Chem Biol, 8, 536–46
  • Lee R, Smirnoff N, Aves SJ, Love J. (2013). Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli. Proc Natl Acad Sci USA, 110, 7636–41
  • Lennen RM, Pfleger BF. (2012). Engineering Escherichia coli to synthesize free fatty acids. Trends Biotechnol, 30, 659–67
  • Li Z, Li X, Wang Y, et al. (2011). Expression and characterization of recombinant Rhizopus oryzae lipase for enzymatic biodiesel production. Bioresour Technol, 102, 9810–13
  • Li AT, Ngo Thao PN, et al. (2012). Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresour Technol, 114, 725–9
  • Liu JF, Nie KL, Fan LH, et al. (2013). Increased production of FAEEs for biodiesel with lipase enhanced Saccharomyces cerevisiae. Process Biochem, 48, 1212–15
  • Lubertozzi D, Keasling JD. (2009). Developing Aspergillus as a host for heterologous expression. Biotechnol Adv, 27, 53–75
  • Matsumoto T, Fukuda H, Ueda M, et al. (2002). Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. Appl Environ Microbiol, 68, 4517–22
  • Matsumoto T, Takahashi S, Kaieda M, et al. (2001). Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production. Appl Microbiol Biotechnol, 57, 515–20
  • Narwal SK, Gupta R. (2013). Biodiesel production by transesterification using immobilized lipase. Biotechnol Lett, 35, 479–90
  • Nawabi P, Bauer S, Kyrpides N, Lykidis A. (2011). Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase. Appl Environ Microbiol, 77, 8052–61
  • Patrick SM, Fazenda ML, McNeil B, Harvey LM. (2005). Heterologous protein production using the Pichia pastoris expression system. Yeast, 22, 249–70
  • Peralta-Yahya PP, Zhang F, del Cardayre SB, Keasling JD. (2012). Microbial engineering for the production of advanced biofuels. Nature, 488, 320–8
  • Rebecca ML, Max AK, Kritika K, et al. (2011). Membrane stresses induced by overproduction of free fatty acids in Escherichia coli. Appl Environ Microbiol, 77, 8114–28
  • Rodrigues RC, Pessela BCC, Volpato G, et al. (2010). Two step ethanolysis: a simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from Thermomyces lanuginosus. Process Biochem, 45, 1268–73
  • Shi S, Valle-Rodríguez OJ, Khoomrung S, et al. (2012). Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production. Biotechnol Biofuels, 5, 7–16
  • Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J. (2011). Prospects for microbial biodiesel production. Biotechnol J, 6, 277–85
  • Steen EJ, Kang Y, Bokinsky G, et al. (2010). Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature, 463, 559–62
  • Stergiou PY, Foukis A, Filippou M, et al. (2013). Advances in lipase-catalyzed esterification reactions. Biotechnol Adv, 31, 1846–59
  • Sun T, Du W, Liu DH. (2008). Prospective and impacts of whole cell mediated alcoholysis of renewable oils for biodiesel production. Biofuel Bioprod Bioref, 3, 633–9
  • Takaya T, Koda R, Adachi D, et al. (2011). Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae. Appl Microbiol Biotechnol, 90, 1171–7
  • Tan TW, Lu JK, Nie KL, et al. (2010). Biodiesel production with immobilized lipase: a review. Biotechnol Adv, 28, 628–34
  • Torella JP, Ford TJ, Kim SN, et al. (2013). Tailored fatty acid synthesis via dynamic control of fatty acid elongation. Proc Natl Acad Sci USA, 110, 11290–5
  • Valle-Rodríguez JO, Shi S, Siewers V, Nielsen J. (2014). Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways. Appl Energy, 115, 226–32
  • Wang B, Lin H, Zhan J, et al. (2012). Biodiesel synthesis by a one-step method in a genetically engineered Escherichia coli using rice straw hydrolysate and restaurant oil wastes as raw materials. J Appl Microbiol, 113, 531–40
  • Xu P, Gu Q, Wang W, et al. (2013). Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun, 4, 1409–16
  • Xu P, Wang W, Li L, et al. (2014). Design and kinetic analysis of a hybrid promoter-regulator system for malonyl-CoA sensing in Escherichia coli. ACS Chem Biol, 9, 451–458
  • Yan JY, Du L, Zheng XL, Li SY. (2014a). Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts. Biotechnol Biofuels, 7, 55–62
  • Yan JY, Gui XH, Wang GL, Yan YJ. (2012a). Improving stability and activity of cross-linked enzyme aggregates based on polyethylenimine in hydrolysis of fish oil for enrichment of polyunsaturated fatty acids. Appl Biochem Biotechnol, 166, 925–32
  • Yan JY, Li LF, Tang QL, Jiang MZ. (2010). Preparation of a crosslinked bioimprinted lipase for enrichment of polyunsaturated fatty acids from fish processing waste. Appl Biochem Biotechnol, 162, 757–65
  • Yan JY, Li AT, Xu Y, et al. (2012b). Efficient production of biodiesel from waste grease: one-pot esterification and transesterification with tandem lipases. Bioresour Technol, 123, 332–7
  • Yan JY, Liu SX, Hu J, et al. (2011a). Enzymatic enrichment of polyunsaturated fatty acids using novel lipase preparations modified by combination of immobilization and fish oil treatment. Bioresour Technol, 102, 7154–8
  • Yan YJ, Xu L, Dai M. (2012c). A synergetic whole-cell biocatalyst for biodiesel production. RSC Adv, 2, 6170–3
  • Yan JY, Yan YJ, Liu SX, et al. (2011b). Preparation of cross-linked lipase-coated micro-crystals for biodiesel production from waste cooking oil. Bioresour Technol, 102, 4755–8
  • Yan JY, Zheng XL, Li SY. (2014b). A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a Thermomyces lanuginosus lipase: preparation, characterization and application in biodiesel production. Bioresour Technol, 151, 43–8
  • Yang L, Zhu Z, Wang WH, Lu XF. (2013). Microbial recycling of glycerol to biodiesel. Bioresour Technol, 150, 1–8
  • Yu KO, Jung J, Kim SW, et al. (2012). Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase. Biotechnol Bioeng, 109, 110–15
  • Zhang F, Carothers JM, Keasling JD. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nat Biotechnol, 30, 354–9

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