Advances in Candida detection platforms for clinical and point-of-care applications

References

• De Hoog G, Guarro J, Gene J, et al. Atlas of clinical fungi Centraalbureau voor Schimmelcultures. Universitat Rovira i Virgili, Amer Society for Microbiology. 2000;164–174.
   Google Scholar
• Fridkin SK, Jarvis WR. Epidemiology of nosocomial fungal infections. Clin Microbiol Rev. 1996;9:499–511.
   PubMed Web of Science ®Google Scholar
• Guarro J, Gené J, Stchigel AM. Developments in fungal taxonomy. Clin Microbiol Rev. 1999;12:454–500.
   PubMed Web of Science ®Google Scholar
• Phaff HJ, Yeasts eLS, 2001;1–11.
   Google Scholar
• Kulp K. Handbook of Cereal Science and Technology, Revised and Expanded: CRC; 2000.
   Google Scholar
• Christian V, Shrivastava R, Shukla D, et al. Degradation of xenobiotic compounds by lignin-degrading white-rot fungi: enzymology and mechanisms involved. Indian J Exp Biol 2005;43:301.
   PubMedGoogle Scholar
• Fincham J. Transformation in fungi. Microbiol Rev. 1989;53:148–170.
   PubMedGoogle Scholar
• Hawkins KM, Smolke CD. Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiae. Nat Chem Biol. 2008;4:564–573.
   PubMed Web of Science ®Google Scholar
• Huang B, Guo J, Yi B, et al. Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae. Biotechnol Lett. 2008;30:1121–1137.
   PubMed Web of Science ®Google Scholar
• Joseph B, Ramteke PW, Thomas G. Cold active microbial lipases: some hot issues and recent developments. Biotechnol Adv. 2008;26:457–470.
   PubMed Web of Science ®Google Scholar
• Kendrick B. Fungi: ecological importance and impact on humans. eLS. 2001.
   Google Scholar
• Murray PR, Rosenthal KS, Pfaller MA. Opportunistic mycoses/candidiasis. Medical microbiology. 44. 7th ed. Philadelphia, PA: Elsevier Saunders; 2013. p. 6776–6783.
   Google Scholar
• Pappas PG. Opportunistic fungi: a view to the future. Am J Med Sci. 2010;340:253–257.
   PubMed Web of Science ®Google Scholar
• Michallet M, Ito JI. Approaches to the management of invasive fungal infections in hematologic malignancy and hematopoietic cell transplantation. J Clin Oncol. 2009;27:3398–3409.
   PubMed Web of Science ®Google Scholar
• Baddley JW, Stroud TP, Salzman D, et al. Invasive mold infections in allogeneic bone marrow transplant recipients. Clin Infect Dis. 2001;32:1319–1324.
   PubMed Web of Science ®Google Scholar
• Person AK, Kontoyiannis DP, Alexander BD. Fungal infections in transplant and oncology patients. Hematol Oncol Clin North Am. 2011;25:193–213.
   PubMed Web of Science ®Google Scholar
• Kaufman D. Fungal infections in neonates: update on prevention and treatment. Minerva Ginecol 2007;59:311–329.
   PubMedGoogle Scholar
• Smith JA, Kauffman CA. Recognition and prevention of nosocomial invasive fungal infections in the intensive care unit. Crit Care Med. 2010;38:S380–S387.
   Google Scholar
• Lionakis MS, Kontoyiannis DP. Glucocorticoids and invasive fungal infections. The Lancet. 2003;362:1828–1838.
   PubMed Web of Science ®Google Scholar
• Sullivan D, Coleman D. Candida dubliniensis: characteristics and identification. J Clin Microbiol. 1998;36:329–334.
   PubMed Web of Science ®Google Scholar
• Zilberberg MD, Shorr AF, Kollef MH. Secular trends in candidemia-related hospitalization in the United States, 2000-2005. Infect Control Hosp Epidemiol. 2008;29:978–980.
   PubMed Web of Science ®Google Scholar
• Ferwerda G, Meyer‐Wentrup F, Kullberg BJ, et al. Dectin-1 synergizes with TLR2 and TLR4 for cytokine production in human primary monocytes and macrophages. Cell Microbiol. 2008;10:2058–2066.
   PubMed Web of Science ®Google Scholar
• Neely LA, Audeh MJ, Blanco M, et al. Inventors; T2 Biosystems, Inc., assignee. NMR systems and methods for the rapid detection of analytes. United States patent US8409807 B2. 2012.
   Google Scholar
• Cheng IF, Chang HC, Hou D, et al. An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting. Biomicrofluidics. 2007;1:021503.
   PubMed Web of Science ®Google Scholar
• Naglik JR, Challacombe SJ, Hube B. Candida albicans secreted aspartyl proteinases in virulence and pathogenesis. Microbiol Mol Biol Rev. 2003;67:400–428.
   PubMed Web of Science ®Google Scholar
• Whiteway M, Oberholzer U. Candida morphogenesis and host-pathogen interactions. Curr Opin Microbiol. 2004;7:350–357.
   PubMed Web of Science ®Google Scholar
• Jarvis WR. Epidemiology of nosocomial fungal infections, with emphasis on Candida species. Clin Infect Dis. 1995;20:1526–1530.
   PubMed Web of Science ®Google Scholar
• Jarvis WR, Martone WJ. Predominant pathogens in hospital infections. J Antimicrob Chemother. 1992;29:19.
   PubMed Web of Science ®Google Scholar
• Richards MJ, Edwards JR, Culver DH, et al. Nosocomial infections in medical intensive care units in the United States. Crit Care Med. 1999;27:887.
   PubMed Web of Science ®Google Scholar
• Richards MJ, Edwards JR, Culver DH, et al. Nosocomial infections in combined medical-surgical intensive care units in the United States. Infect Control Hosp Epidemiol. 2000;21:510–515.
   PubMed Web of Science ®Google Scholar
• Edmond MB, Wallace SE, McClish DK, et al. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis. 1999;29:239–244.
   PubMed Web of Science ®Google Scholar
• Villamizar RA, Maroto A, Riusa FX, et al. Fast detection of Salmonella Infantis with carbon nanotube field effect transistors. Biosens Bioelectron 2008;24:279–283.
   PubMed Web of Science ®Google Scholar
• Hall KK, Lyman JA. Updated review of blood culture contamination. Clin Microbiol Rev. 2006;19:788–802.
   PubMed Web of Science ®Google Scholar
• Morgan J, Meltzer MI, Plikaytis BD, et al. Excess mortality, hospital stay, and cost due to candidemia: a case-control study using data from population-based candidemia surveillance. Infect Control. 2005;26:540–547.
   Google Scholar
• Wey SB, Mori M, Pfaller MA, et al. Hospital-acquired candidemia. The attributable mortality and excess length of stay. Arch Inter Med. 1988;148:2642
   PubMed Web of Science ®Google Scholar
• Fraser VJ, Jones M, Dunkel J, et al. Candidemia in a tertiary care hospital: epidemiology, risk factors, and predictors of mortality. Clin Infect Dis. 1992;15:414–421.
   PubMed Web of Science ®Google Scholar
• Leleu G, Aegerter P, Guidet B. Systemic candidiasis in intensive care units: a multicenter, matched-cohort study. J Critical Care. 2002;17:168–175.
   PubMed Web of Science ®Google Scholar
• Méan M, Marchetti O, Calandra T. Bench-to-bedside review: Candida infections in the intensive care unit. Crit Care. 2008;12:204.
   PubMed Web of Science ®Google Scholar
• Saghrouni F, Ben Abdeljelil J, Boukadida J, et al. Molecular methods for strain typing of Candida albicans: a review. J Appl Microbiol. 2013;114:1559–1574.
   PubMed Web of Science ®Google Scholar
• Jain N, Mathur P, Misra MC, et al. Rapid identification of yeast isolates from clinical specimens in critically Ill trauma ICU patients. J Lab Physicians. 2012;4:30.
   PubMedGoogle Scholar
• Zilberberg MD, Kollef MH, Arnold H, et al. Inappropriate empiric antifungal therapy for candidemia in the ICU and hospital resource utilization: a retrospective cohort study. BMC Infect Dis. 2010;10:150.
   PubMed Web of Science ®Google Scholar
• Rex JH, Pfaller MA, Barry AL, et al. Antifungal susceptibility testing of isolates from a randomized, multicenter trial of fluconazole versus amphotericin B as treatment of nonneutropenic patients with candidemia. NIAID Mycoses study group and the Candidemia study group. Antimicrob Agents Chemother. 1995;39:40–44.
   PubMed Web of Science ®Google Scholar
• Wingard JR, Merz WG, Rinaldi MG, et al. Association of Torulopsis glabrata infections with fluconazole prophylaxis in neutropenic bone marrow transplant patients. Antimicrob Agents Chemother. 1993;37:1847–1849.
   PubMed Web of Science ®Google Scholar
• Canuto MM, Rodero FG. Antifungal drug resistance to azoles and polyenes. Lancet Infect Dis. 2002;2:550–563.
   PubMed Web of Science ®Google Scholar
• Rangel-Frausto MS, Wiblin T, Blumberg HM, et al. National epidemiology of mycoses survey (NEMIS): variations in rates of bloodstream infections due to Candida species in seven surgical intensive care units and six neonatal intensive care units. Clin Infect Dis. 1999;29:253–258.
   PubMed Web of Science ®Google Scholar
• Pfaller MA, Diekema DJ, Jones RN, et al. International surveillance of bloodstream infections due to Candida species: frequency of occurrence and in vitro susceptibilities to fluconazole, ravuconazole, and voriconazole of isolates collected from 1997 through 1999 in the SENTRY antimicrobial surveillance program. J Clin Microbiol. 2001;39:3254–3259.
   PubMed Web of Science ®Google Scholar
• Agarwal S, Manchanda V, Verma N, et al. Yeast identification in routine clinical microbiology laboratory and its clinical relevance. Indian J Med Microbiol. 2012;29:172.
   Web of Science ®Google Scholar
• Larone DH. Medically important fungi: a guide to identification. 2nd ed. New York: Elsevier Science Publishing Co; 1987.
   Google Scholar
• Koneman EW, Roberts GD. Practical laboratory mycology. Baltimore, MD: Williams & Wilkins; 1985.
   Google Scholar
• Adams AEM, Stearns TIM. Fluorescence microscopy methods for yeast. Methods in cell biology, volume 31: Vesicular transport, Part A: Vesicular transport. 1989:357.
   Google Scholar
• Hageage GJ, Harrington BJ. Use of calcofluor white in clinical mycology. Lab Med. 1984;15:109–112.
   Google Scholar
• Perry JL, Miller GR. Quality control slide for potassium hydroxide and cellufluor fungal preparations. J Clin Microbiol. 1989;27:1411–1412.
   PubMed Web of Science ®Google Scholar
• Ruiz-Herrera J. Fungal cell wall: structure, synthesis, and assembly. Boca Raton, Florida: CRC press; 1991.
   Google Scholar
• Harrington BJ, Hageage GJ. Calcofluor white: a review of its uses and applications in clinical mycology and parasitology. Lab Med. 2003;34:361–367.
   Web of Science ®Google Scholar
• Harrington BJ, Williams DL. Rapid, presumptive identification of Torulopsis (Candida) Glabrata and Candida Krusei using Calcofluor White. Lab Med. 2007;38:227–231.
   Web of Science ®Google Scholar
• Baselski VS, Robison MK, Pifer LW, et al. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol. 1990;28:393–394.
   PubMed Web of Science ®Google Scholar
• Sautter RL, Kwee HG. Calcofluor white stain for fungi. Am J Clin Pathol. 1987;87:295.
   PubMed Web of Science ®Google Scholar
• Lischewski A, Kretschmar M, Hof H, et al. Detection and identification of Candida species in experimentally infected tissue and human blood by rRNA-specific fluorescent in situ hybridization. J Clin Microbiol. 1997;35:2943–2948.
   PubMed Web of Science ®Google Scholar
• Bisha B, Kim HJ, Brehm-Stecher BF. Improved DNA-FISH for cytometric detection of Candida spp. J Appl Microbiol. 2011;110:881–892.
   PubMed Web of Science ®Google Scholar
• Rigby S, Procop GW, Haase G, et al. Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles. J Clin Microbiol. 2002;40:2182–2186.
   PubMed Web of Science ®Google Scholar
• Trnovsky J, Merz W, Della-Latta P, et al. Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow. J Clin Microbiol. 2008;46:1537–1540.
   PubMed Web of Science ®Google Scholar
• Gherna M, Merz WG. Identification of Candida albicans and Candida glabrata within 1.5 hours directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol. J Clin Microbiol. 2009;47:247–248.
   PubMed Web of Science ®Google Scholar
• Borekci G, Ersoz G, Otag F, et al. Identification of Candida species from blood cultures with fluorescent in situ hybridization (FISH), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and conventional methods. Balkan Med J. 2010;27:183–191.
   Google Scholar
• Kempf VAJ, Trebesius K, Autenrieth IB. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J Clin Microbiol. 2000;38:830–838.
   PubMed Web of Science ®Google Scholar
• Rij KV. The yeasts. A taxonomic study. New York, NY: Elsevier Science Publishers BV; 1984.
   Google Scholar
• Espinel-Ingroff A, Stockman L, Roberts G, et al. Comparison of RapID yeast plus system with API 20C system for identification of common, new, and emerging yeast pathogens. J Clin Microbiol. 1998;36:883–886.
   PubMed Web of Science ®Google Scholar
• Davey KG, Chant PM, Downer CS, et al. Evaluation of the AUXACOLOR system, a new method of clinical yeast identification. J Clin Pathol. 1995;48:807–809.
   PubMed Web of Science ®Google Scholar
• El-Zaatari M, Pasarell L, McGinnis MR, et al. Evaluation of the updated Vitek yeast identification data base. J Clin Microbiol. 1990;28:1938–1941.
   PubMed Web of Science ®Google Scholar
• Fenn JP, Segal H, Barland B, et al. Comparison of updated Vitek yeast biochemical card and API 20C yeast identification systems. J Clin Microbiol.1994;32:1184–1187.
   PubMed Web of Science ®Google Scholar
• Campbell CK, Davey KG, Holmes AD, et al. Comparison of the API Candida system with the AUXACOLOR system for identification of common yeast pathogens. J Clin Microbiol. 1999;37:821–823.
   PubMed Web of Science ®Google Scholar
• Verweij PE, Breuker IM, Rijs AJ, et al. Comparative study of seven commercial yeast identification systems. J Clin Pathol. 1999;52:271–273.
   PubMed Web of Science ®Google Scholar
• Koehler AP, Chu KC, Houang ETS, et al. Simple, reliable, and cost-effective yeast identification scheme for the clinical laboratory. J Clin Microbiol. 1999;37:422–426.
   PubMed Web of Science ®Google Scholar
• Willinger B, Manafi M. Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species. Mycoses. 2002;42:61–65.
   Web of Science ®Google Scholar
• Adam HJ, Richardson SE, Roscoe M, et al. An implementation strategy for the use of chromogenic media in the rapid, presumptive identification of Candida species. Open Mycol J. 2010;4:33–38.
   Google Scholar
• Pfaller MA, Houston A, Coffmann S. Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol. 1996;34:58–61.
   PubMed Web of Science ®Google Scholar
• Sivakumar VG, Shankar P, Nalina K, et al. Use of CHROMagar in the differentiation of common species of Candida. Mycopathologia. 2009;167:47–49.
   PubMed Web of Science ®Google Scholar
• Sudbery P, Gow N, Berman J. The distinct morphogenic states of Candida albicans. Trends Microbiol. 2004;12:317–324.
   PubMed Web of Science ®Google Scholar
• Whiteway M, Bachewich C. Morphogenesis in Candida albicans*. Annu Rev Microbiol. 2007;61:529–553.
   PubMed Web of Science ®Google Scholar
• Kadosh D, Johnson AD. Induction of the Candida albicans filamentous growth program by relief of transcriptional repression: a genome-wide analysis. Mol Biol Cell. 2005;16:2903–2912.
   PubMed Web of Science ®Google Scholar
• Nakamoto S. Germ tube formation of Candida albicans in corn meal broth using the non-slip slide glass incubation method. Yonago Acta Medica. 1998;41:65–72.
   Google Scholar
• Sahand IH, Maza JL, Eraso E, et al. Evaluation of CHROM-Pal medium for the isolation and direct identification of Candida dubliniensis in primary cultures from the oral cavity. J Med Microbiol. 2009;58:1437–1442.
   PubMed Web of Science ®Google Scholar
• Horvath LL, Hospenthal DR, Murray CK, et al. Direct isolation of Candida spp. from blood cultures on the chromogenic medium CHROMagar Candida. J Clin Microbiol. 2003;41:2629–2632.
   PubMed Web of Science ®Google Scholar
• BacT/ALERT® MB, BioMerieux. [cited 2015 Dec 26]. Available from: http://www.biomerieux-usa.com/clinical/bact-alert-3d-healthcare.
   Google Scholar
• TREK Diagnostic System. [cited 2015 Feb 04]. Available from: http://www.trekds.com/products/versatrek/media.asp.
   Google Scholar
• João Inácio OFaIS-M. Efficient identification of clinically relevant Candida yeast species by use of an assay combining Panfungal loop-mediated isothermal DNA amplification with hybridization to species-specific oligonucleotide probes. J Clin Microbiol. 2008;46:713–720.
   PubMed Web of Science ®Google Scholar
• Clancy CJ, Nguyen ML, Cheng S, et al. Immunoglobulin G responses to a panel of Candida albicans antigens as accurate and early markers for the presence of systemic candidiasis. J Clin Microbiol. 2008;46:1647–1654.
   PubMed Web of Science ®Google Scholar
• Medical microbiology. 7th ed. Philadelphia, PA: Elsevier Saunders; 2013.
   Google Scholar
• Reiss E, De Repentigny L, Kuykendall R, et al. Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence. J Clin Microbiol. 1986;24:796–802.
   PubMed Web of Science ®Google Scholar
• Philip A, Odabasi Z, Matiuzzi G, et al. Syscan3, a kit for detection of anti-candida antibodies for diagnosis of invasive candidiasis. J Clin Microbiol. 2005;43:4834–4835.
   PubMed Web of Science ®Google Scholar
• Mitsutake K, Miyazaki T, Tashiro T, et al. Enolase antigen, mannan antigen, Cand-Tec antigen, and beta-glucan in patients with candidemia. J Clin Microbiol. 1996;34:1918–1921.
   PubMed Web of Science ®Google Scholar
• Stickle D, Kaufman L, Blumer SO, et al. Comparison of a newly developed latex agglutination test and an immunodiffusion test in the diagnosis of systemic candidiasis. Appl Microbiol. 1972;23:490–499.
   PubMedGoogle Scholar
• Marot-Leblond A, Beucher B, David S, et al. Development and evaluation of a rapid latex agglutination test using a monoclonal antibody to identify Candida dubliniensis colonies. J Clin Microbiol. 2006;44:138–142.
   PubMed Web of Science ®Google Scholar
• Fujita S, Hashimoto T. Detection of serum Candida antigens by enzyme-linked immunosorbent assay and a latex agglutination test with anti-Candida albicans and anti-Candida krusei antibodies. J Clin Microbiol. 1992;30:3132–3137.
   PubMed Web of Science ®Google Scholar
• Matthews R, Burnie J. Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol. 1988;26:459–463.
   PubMed Web of Science ®Google Scholar
• Manning-Zweerink M, Maloney C, Mitchell T, et al. Immunoblot analyses of Candida albicans-associated antigens and antibodies in human sera. J Clin Microbiol. 1986;23:46–52.
   PubMed Web of Science ®Google Scholar
• Kostiala I, Kostiala A, Larinkari U, et al. Antibodies against antigens of Candida albicans in patients with fungaemia and bacteraemia, studied by ELISA, precipitation, passive haemagglutination and immunofluorescence techniques. J Med Microbiol. 1981;14:483–492.
   PubMed Web of Science ®Google Scholar
• Fujita S, Matsubara F, Matsuda T. Enzyme-linked immunosorbent assay measurement of fluctuations in antibody titer and antigenemia in cancer patients with and without candidiasis. J Clin Microbiol. 1986;23:568–575.
   PubMed Web of Science ®Google Scholar
• Laín A, Elguezabal N, Brena S, et al. Diagnosis of invasive candidiasis by enzyme-linked immunosorbent assay using the N-terminal fragment of Candida albicans hyphal wall protein 1. BMC Microbiol. 2007;7:35.
   PubMed Web of Science ®Google Scholar
• Laín A, Moragues MD, Ruiz JCG, et al. Evaluation of a novel enzyme-linked immunosorbent assay to detect immunoglobulin G antibody to enolase for serodiagnosis of invasive candidiasis. Clin Vaccine Immunol. 2007;14:318–319.
   PubMed Web of Science ®Google Scholar
• Van Deventer A, Van Vliet H, Hop W, et al. Diagnostic value of anti-Candida enolase antibodies. J Clin Microbiol. 1994;32:17–23.
   PubMed Web of Science ®Google Scholar
• Pisa D, Ramos M, Molina S, et al. Evolution of antibody response and fungal antigens in the serum of a patient infected with Candida famata. J Med Microbiol. 2007;56:571–578.
   PubMed Web of Science ®Google Scholar
• Strockbine NA, Largen MT, Buckley HR. Production and characterization of three monoclonal antibodies to Candida albicans proteins. Infect Immun. 1984;43:1012–1018.
   PubMed Web of Science ®Google Scholar
• Ponton J, Marot-Leblond A, Ezkurra P, et al. Characterization of Candida albicans cell wall antigens with monoclonal antibodies. Infect Immun. 1993;61:4842–4847.
   PubMed Web of Science ®Google Scholar
• Schneider J, Moragues D, MartÃnez N, et al. Cross-reactivity between Candida albicans and human ovarian carcinoma as revealed by monoclonal antibodies PA10F and C6. Br J Cancer. 1998;77:1015.
   PubMed Web of Science ®Google Scholar
• Rodriguez MJ, Schneider J, Moragues MD, et al. Cross-reactivity between Candida albicans and oral squamous cell carcinoma revealed by monoclonal antibody C7. Anticancer Res. 2007;27:3639–3643.
   PubMed Web of Science ®Google Scholar
• Kaufman L, Kovacs JA, Reiss E. Clinical immunomycology. Manual of clinical laboratory immunology. Washington, DC: American Society for Microbiology; 1997:575–583.
   Google Scholar
• Marcilla A, Monteagudo C, Mormeneo S, et al. Monoclonal antibody 3H8: a useful tool in the diagnosis of candidiasis. Microbiology. 1999;145:695–701.
   PubMed Web of Science ®Google Scholar
• Yeo SF, Wong B. Current status of nonculture methods for diagnosis of invasive fungal infections. Clin Microbiol Rev. 2002;15:465–484.
   PubMed Web of Science ®Google Scholar
• Jarvensivu A, Hietanen J, Rautemaa R, et al. Candida yeasts in chronic periodontitis tissues and subgingival microbial biofilms in vivo. Oral Dis. 2004;10:106–112.
   PubMed Web of Science ®Google Scholar
• Bikandi J, San Millán R, Moragues MD, et al. Rapid identification of Candida dubliniensis by indirect immunofluorescence based on differential localization of antigens on C. dubliniensis blastospores and Candida albicans germ tubes. J Clin Microbiol. 1998;36:2428–2433.
   PubMed Web of Science ®Google Scholar
• Jarvensivu A, Rautemaa R, Sorsa T, et al. Specificity of the monoclonal antibody 3H8 in the immunohistochemical identification of Candida species. Oral Dis. 2006;12:428–433.
   PubMed Web of Science ®Google Scholar
• Chang CM, Chang WH, Wang CH, et al. Nucleic acid amplification using microfluidic systems. Lab Chip. 2013;13:1225–1242.
   PubMed Web of Science ®Google Scholar
• Simon D, Atkins IMC. Fungal molecular diagnostics: a mini review. J Appl Genet. 2004;45:3–15.
   PubMedGoogle Scholar
• Arvanitis M, Anagnostou T, Fuchs BB, et al. Molecular and nonmolecular diagnostic methods for invasive fungal infections. Clin Microbiol Rev. 2014;27:490–526.
   PubMed Web of Science ®Google Scholar
• Zourob M, Elwary S, Turner AP. Principles of bacterial detection: biosensors, recognition receptors and microsystems. New York (NY): Springer Science & Business Media; 2008.
   Google Scholar
• Campa D, Tavanti A, Gemignani F, et al. DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. J Clin Microbiol. 2008;46:909–915.
   PubMed Web of Science ®Google Scholar
• Spiess B, Seifarth W, Hummel M, et al. DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. J Clin Microbiol. 2007;45:3743–3753.
   PubMed Web of Science ®Google Scholar
• Leinberger DM, Schumacher U, Autenrieth IB, et al. Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses. J Clin Microbiol. 2005;43:4943–4953.
   PubMed Web of Science ®Google Scholar
• Reiss E, Tanaka K, Bruker G, et al. Molecular diagnosis and epidemiology of fungal infections. Med Mycol. 1997;36:249–257.
   Web of Science ®Google Scholar
• Mullis K, Faloona F, Scharf S, et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol. 1986;51:263–273.
   PubMedGoogle Scholar
• Kan VL. Polymerase chain reaction for the diagnosis of candidemia. J Infect Dis. 1993;168:779–783.
   PubMed Web of Science ®Google Scholar
• Jordan JA. PCR identification of four medically important Candida species by using a single primer pair. J Clin Microbiol. 1994;32:2962–2967.
   PubMed Web of Science ®Google Scholar
• Einsele H, Hebart H, Roller G, et al. Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol. 1997;35:1353–1360.
   PubMed Web of Science ®Google Scholar
• Burnie JP, Golband N, Matthews RC. Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis. Eur J Clin Microbiol Dis. 1997;16:346–350.
   PubMed Web of Science ®Google Scholar
• Widjojoatmodjo MN, Borst A, Schukkink RAF, et al. Nucleic acid sequence-based amplification (NASBA) detection of medically important Candida species. J Microbiol Meth. 1999;38:81–90.
   PubMed Web of Science ®Google Scholar
• Zhou X, Kong F, Sorrell TC, et al. Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification▿. J Clin Microbiol. 2008;46:2423–2427.
   PubMed Web of Science ®Google Scholar
• Prasad JK, Feller I, Thomson PD. A ten-year review of Candida sepsis and mortality in burn patients. Surgery. 1987;101:213–216.
   PubMed Web of Science ®Google Scholar
• Kurita H, Kamata T, Zhao C, et al. Usefulness of a commercial enzyme-linked immunosorbent assay kit for Candida mannan antigen for detecting Candida in oral rinse solutions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;107:531–534.
   PubMed Web of Science ®Google Scholar
• Lehmann LE, Hunfeld K-P, Emrich T, et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol. 2008;197:313–324.
   PubMed Web of Science ®Google Scholar
• Ilyas A, Asghar W, Allen PB, et al. Electrical detection of cancer biomarker using aptamers with nanogap break-junctions. Nanotechnology. 2012;23:275502.
   PubMed Web of Science ®Google Scholar
• Wan Y, Tan J, Asghar W, et al. Velocity effect on aptamer-based circulating tumor cell isolation in microfluidic devices. J Phys Chem B. 2011;115:13891–13896.
   PubMed Web of Science ®Google Scholar
• Pulcrano G, Iula DV, Vollaro A, et al. Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections. J Microbiol Methods. 2013;94:262–266.
   PubMed Web of Science ®Google Scholar
• Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507:181–189.
   PubMed Web of Science ®Google Scholar
• Asghar W, El Assal R, Shafiee H, et al. Preserving human cells for regenerative, reproductive, and transfusion medicine. Biotechnol J. 2014;9:895–903.
   PubMed Web of Science ®Google Scholar
• Asghar W, El Assal R, Shafiee H, et al. Engineering cancer microenvironments for in vitro 3-D tumor models. Mater Today. 2015;18:539–553.
   Web of Science ®Google Scholar
• Asghar W, Ramachandran PP, Adewumi A, et al. Rapid nanomanufacturing of metallic break junctions using focused ion beam scratching and electromigration. J Manufact Sci Eng. 2010;132:030911.
   Web of Science ®Google Scholar
• Asghar W, Velasco V, Kingsley JL, et al. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater. 2014;3:1671–1679.
   PubMed Web of Science ®Google Scholar
• Asghar W, Wan Y, Ilyas A, et al. Electrical fingerprinting, 3D profiling and detection of tumor cells with solid-state micropores. Lab on a Chip. 2012;12:2345–2352.
   PubMed Web of Science ®Google Scholar
• Ilyas A, Asghar W, Ahmed S, et al. Electrophysiological analysis of biopsy samples using elasticity as an inherent cell marker for cancer detection. Anal Methods. 2014;6:7166–7174.
   Web of Science ®Google Scholar
• Ilyas A, Asghar W, Kim YT, et al. Parallel recognition of cancer cells using an addressable array of solid-state micropores. Biosens Bioelectron. 2014;62:343–349.
   PubMed Web of Science ®Google Scholar
• Wang L, Asghar W, Demirci U, et al. Nanostructured substrates for isolation of circulating tumor cells. Nano today. 2013;8:374–387.
   Web of Science ®Google Scholar
• Asghar W, Islam M, Wadajkar AS, et al. PLGA micro-and nanoparticles loaded into gelatin scaffold for controlled drug release. IEEE Trans Nanotechnol. 2012;11:546–553.
   Web of Science ®Google Scholar
• Ramachandran A, Liu Y, Asghar W, et al. Characterization of DNA-nanopore interactions by molecular dynamics. Am J Biomed Sci. 2009;1:344–351.
   Google Scholar
• Vidyala SD, Asghar W, Iqbal SM. Porous organic nanolayers for coating of solid-state devices. J Nanobiotechnol. 2011;9:18.
   PubMed Web of Science ®Google Scholar
• Ilyas A, Islam M, Asghar W, et al. Salt-leaching synthesis of porous PLGA nanoparticles. IEEE Trans Nanotechnol. 2013;12:1082–1088.
   Web of Science ®Google Scholar
• Ilyas A, Asghar W, Billo J, et al. From molecular electronics to proteonics: break junctions for biomarker detection. Life Science Systems and Applications Workshop (LiSSA), 2011 IEEE/NIH; 2011: Bethesda, MD: IEEE.
   Google Scholar
• Sachse S, Straube E, Lehmann M, et al. Truncated human cytidylate-phosphatedeoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples. J Clin Microbiol. 2009;47:1050–1057.
   PubMed Web of Science ®Google Scholar
• Mylonakis E, Clancy CJ, Ostrosky-Zeichner L, et al. T2 magnetic resonance assay for the rapid diagnosis of candidemia in whole blood: a clinical trial. Clin Infect Dis. 2015;60:892–899.
   PubMed Web of Science ®Google Scholar
• Baeumner SJ. Microfluidic isolation of nucleic acids. Angew Chem Int Ed. 2014;53:2–16.
   Web of Science ®Google Scholar
• Cai D, Xiao M, Xu P, et al. An integrated microfluidic device utilizing dielectrophoresis and multiplex array PCR for point-of-care detection of pathogens. Lab Chip. 2014;14:3917–3924.
   PubMed Web of Science ®Google Scholar
• Boles DJ, Benton JL, Siew GJ, et al. Droplet-based pyrosequencing using digital microfluidics. Anal Chem. 2011;83:8439–8447.
   PubMed Web of Science ®Google Scholar
• Schell WA, Benton JL, Smith PB, et al. Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood. Eur J Microbiol Infect Dis. 2012;31:2237–2245.
   PubMed Web of Science ®Google Scholar
• Schumacher S, Nestler J, Otto T, et al. Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. Lab Chip. 2012;12:464–473.
   PubMed Web of Science ®Google Scholar
• Lee H, Sun E, Ham D, et al. Chip-NMR biosensor for detection and molecular analysis of cells. Nat Med. 2008;14:869–874.
   PubMed Web of Science ®Google Scholar
• Gervais L, de Rooij N, Delamarche E. Microfluidic chips for point-of-care immunodiagnostics. Adv Mater Weinheim. 2011;23:H151–HH76.
   PubMed Web of Science ®Google Scholar
• Shafiee H, Sano MB, Henslee EA, et al. Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP). Lab on a Chip. 2010;10:438–445.
   PubMed Web of Science ®Google Scholar
• Fujita SI, Takamura T, Nagahara M, et al. Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia. J Med Microbiol. 2006;55:537–543.
   PubMed Web of Science ®Google Scholar
• Sogawa K, Watanabe M, Sato K, et al. Use of the MALDI BioTyper system with MALDI-TOF mass spectrometry for rapid identification of microorganisms. Anal Bioanal Chem. 2011;400:1905–1911.
   PubMed Web of Science ®Google Scholar
• Yetisen AK, Akram MS, Lowe CR. Paper-based microfluidic point-of-care diagnostic devices. Lab Chip. 2013;13:2210.
   PubMed Web of Science ®Google Scholar
• Shafiee H, Asghar W, Inci F, et al. Paper and flexible substrates as materials for biosensing platforms to detect multiple biotargets. Scientific Rep. 2015;5:8719.
   PubMed Web of Science ®Google Scholar
• Sista R, Hua Z, Thwar P, et al. Development of a digital microfluidic platform for point of care testing. Lab Chip. 2008;8:2091–2104.
   PubMed Web of Science ®Google Scholar
• Eggimann P, Garbino J, Pittet D. Epidemiology of Candida species infections in critically ill non-immunosuppressed patients. Lancet Infect Dis. 2003;3:685–702.
   PubMed Web of Science ®Google Scholar
• Safavieh M, Kanakasabapathy MK, Tarlan F, et al. Emerging loop-mediated isothermal amplification-based microchip and microdevice technologies for nucleic acid detection. ACS Biomater Sci Eng. 2016;3:278–294.
   Web of Science ®Google Scholar
• Asghar W, Yuksekkaya M, Shafiee H, et al. Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications. Sci Rep. 2016;6:21163.
   PubMed Web of Science ®Google Scholar